Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009385

Physical Location & Seq

Genomic Coordinates Reverse (-)

37512580 .. 37524600

Gene Structure 12021 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00112380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGAATGTTATTGCTGCTCATGTAGGGGAGATGTCCATCGAGACGGGGATTACCATCGAGCGGTTCATGTCTGGATTTTCGCCGAAAGCACGCAAGAACTGCTTATCCAGAAACGTGCTGACTTCAAGGATTCTTTTGCCGGCTTGTGGGACATCTCCAGTGCTGGACACATATCCGCCGGTGATTCGTCGCTTATCACTGCCCGGTCCTGACCAATTTAGTTATAGTGTCGTTTTAGTAGTCTCGCTGCGTTGGAGGGAGCTTGAAGAGGAGCTAGGTATAATACTTCCAAAAGATGCATTTGAAATGATCTTCGTATTTCTTCAAGAGTGTGTAATAAATGATGGAAAATTCATCAATAATGAATTCAATGATGTATACTTGGTTACAACTCTAGATCCTATCCCTCTGGAGGCATTTACTCTTCAGGAAACAGAAGTCTCTGCTGTTAAATATATTTCTTATGAGGAGTATAAAAGCTTACTAGCTAAAGAAGATCCCGAGTACGTACCCTATGATGTAAATGGGCAGTATGGTCAATTGTTTGACATTATTGCACGGAGGTACAAGGAGAACACTACAGCTCGAAGTTTGTCACTGCAAAAGCAACTCCAACGTTATGCTCCTGTTACCCTCAATCCAGAGTTGACAGGGCTCACAGATGCAGATAAGGAGGCTCTTGTTATACTTGTTAAGGCTGCAACTATTTTGGATGAGATCTTTCACCTGCAGGTGTGGTACAGTAATCCAGCTCTAAGAGATTGGTTGAAGGAGCATGCAGATGCATCACAGATGGACAACTTGAAATGGATGTATTATTTAATTAATAAGAGTCCATGGTCGTGCCTTGATGAAAATGAGGCATTTTTGACAACTGCAGACTCAGCTGTACAGTTGCTTCCTGAAGCAACGAAGCCTGTTACTGGTTGGAAGGGACTTGAATATAGAGCTGCATTTCCTATGCTAAAACCACCGGGGGCAAACTTCTATCCTCCAGACATGGACAAAACGGAATTTGAATTTTGGAAGAGTAGTCTAACAGTGGACCAACAAGAAGATGCAACAGGCTTTTTCAATGTTATTAAAAGACACAGTGAATCTAGTCTAGATTCCACTTTAAATGGTAATACATTTGGAAGCAAAAACCATTCTGTGGGTTCGACTCCTGACTTGTACACTATCCCTTTCTCCCAAGAGTATAACACTTTCCTCACACAAGCTGCTGAATTATTGCATAAAGCAGGTGACTTGACCAGTTCACCTAGTTTGAAGAGGTTGCTTCACAGCAAGGGCGATGCTTTCCTATCAAATGATTACTATGACTCAGATATAGCCTGGATGGAGTTGGACTCTAAGCTGGATGTCACTATAGGTCCATACGAAACATATGAAGATGCACTGTTCGGGTATAAGGCTACATTTGAAGCATTTATTGGAGTTCGGGATGACAAAGCAACGGCTCAGCTAAAACTTTTTGGTGATAACTTGCAGTTTTTGGAGCAAAATCTCCCACTGGACGATGTCTACAAGTCCAAGGAAGTGATTTCTGCTCCTATTCGAGTTATCGAGCTTCTATATAATGCCGGAGATGTAAAGGGCCCTCAAACTGTTGCTTTCAATCTACCAAATGATGAGCGCATTGTAAAAGATCGAGGAACATCTATGGTCATGCTTAAGAATATCTCAGAGGCCAAGTTCAAACATATTCTTCTGCCTATAGCTGATGTTTGTATTACAAAGGAACAACAAGAACTTGTAGATTTTGAATCCTTCTTCACTCACACAATTTGCCATGAATGCTGTCATGGGATTGGACCTCATACCATAACACTTCCAAGTGGTAGAAAGTCTACAGTGAGATTGGAACTGCAAGAACTCCACTCAGCTTTGGAAGAAGCAAAAGCTGATATAGTTGGCCTTTGGGCACTGAAATTCCTAATCCACAAGGAACTGCTCCCGAAGAGCTTATTGAAATCCATGTATGTTTCTTTTCTTGCTGGATGCTTCCGCTCAGTTCGTTTCGGTCTTAAGGAAGCTCATGGAAAAGGACAAGCATTGCAGTTTAACTGGTTGTACAAGCAAGGAGCCTTTATTTTGAATCCTGATGAAACATTTTCTGTTGACTTTGATAAGGTTGAAGGTGCGGTTGAGAGCCTGAGTAGGGAGATACTTACCATACAAGCAAAAGGTGACAAAGAGGCTGCAAAATTGCTTCTTCAGAAACACTGCACAATGACAGACCCATTAAAAGTTGCTTTGCAACGATTAGAAAAAGTTCAGGTACCGGTGGATATAGTTCCCAGATTTCCGGTGGCTGAGAATATATTGATTCAAAATAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008239 GO:0016787 GO:0019538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NUDIX Peptidase_M49

Protein Analysis

780

Amino Acids

88.08

Weight (kDa)

5.15

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M49	PF03571	399 - 605	1.2e-08	Peptidase family M49

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79690 AT1G79690
fragaria_vesca	FvH4_6g28160 FvH4_6g28160 FvH4_6g28160 FvH4_6g28160 FvH4_6g28160 FvH4_6g28160
malus_domestica	MD17G1227300.v1.1
prunus_persica	Prupe.3G104700_v2.0.a1
pyrus_communis	pycom17g23060
rosa_chinensis	RchiOBHm_Chr2g0132121
rosa_laevigata	RLG00000019229
rosa_multiflora	Rmu_sc0002005.1_g000003 Rmu_sc0009538.1_g000018 Rmu_sc0033552.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112380
rosa_rugosa	Rorug02G0302400
rosa_samantha	Rh2AG355500 Rh2BG361600 Rh2CG339000
rosa_wichuraiana	Rw2G028860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	721, 735, 1232
Acc36I	ACCTGC	3 cut(s)	721, 735, 1232
Acc65I	GGTACC	1 cut(s)	2281
AccB1I	GGYRCC	1 cut(s)	2281
AccB7I	CCANNNNNTGG	1 cut(s)	1153
AccBSI	CCGCTC	2 cut(s)	61, 2010
AccI	GTMKAC	3 cut(s)	378, 1524, 1850
AciI	CCGC	4 cut(s)	61, 176, 2008, 2144
AclI	AACGTT	1 cut(s)	616
AclWI	GGATC	2 cut(s)	392, 491
AcsI	RAATTY	5 cut(s)	350, 365, 1013, 1019, 1931
AcuI	CTGAAG	3 cut(s)	410, 924, 2201
AfaI	GTAC	8 cut(s)	506, 510, 566, 740, 891, 1175, 2075, 2283
AfiI	CCNNNNNNNGG	6 cut(s)	60, 146, 412, 923, 1153, 2161
AflII	CTTAAG	2 cut(s)	1673, 2027
AgeI	ACCGGT	1 cut(s)	2284
AjnI	CCWGG	1 cut(s)	1334
AjuI	GAANNNNNNNTTGG	2 cut(s)	1416, 1448
AloI	GAACNNNNNNTCC	2 cut(s)	89, 121
Alw26I	GTCTC	3 cut(s)	35, 247, 445
AlwI	GGATC	2 cut(s)	392, 491
Ama87I	CYCGRG	1 cut(s)	500
AoxI	GGCC	3 cut(s)	1597, 1689, 1915
ApaI	GGGCCC	1 cut(s)	1601
ApeKI	GCWGC	6 cut(s)	14, 247, 698, 950, 1220, 2201
ApoI	RAATTY	5 cut(s)	350, 365, 1013, 1019, 1931
AseI	ATTAAT	1 cut(s)	825
AsiGI	ACCGGT	1 cut(s)	2284
Asp700I	GAANNNNTTC	1 cut(s)	2274
Asp718I	GGTACC	1 cut(s)	2281
AspLEI	GCGC	1 cut(s)	1638
AspS9I	GGNCC	6 cut(s)	206, 1045, 1373, 1597, 1598, 1814
AsuC2I	CCSGG	2 cut(s)	204, 975
AsuHPI	GGTGA	6 cut(s)	193, 716, 1251, 1256, 1490, 2201
AvaI	CYCGRG	1 cut(s)	500
AvaII	GGWCC	4 cut(s)	206, 1045, 1373, 1814
BaeGI	GKGCMC	2 cut(s)	1601, 1927
BanI	GGYRCC	1 cut(s)	2281
BanII	GRGCYC	2 cut(s)	657, 1601
BarI	GAAGNNNNNNTAC	2 cut(s)	882, 914
BbvI	GCAGC	5 cut(s)	234, 685, 937, 1207, 2188
BccI	CCATC	5 cut(s)	44, 62, 338, 787, 1333
BceAI	ACGGC	1 cut(s)	1473
BciT130I	CCWGG	1 cut(s)	1336
BcnI	CCSGG	2 cut(s)	204, 975
BcoDI	GTCTC	3 cut(s)	35, 247, 445
BfaI	CTAG	6 cut(s)	275, 395, 485, 1101, 1106, 1263
BfmI	CTRYAG	6 cut(s)	579, 728, 876, 1368, 1716, 1851
BfrI	CTTAAG	2 cut(s)	1673, 2027
BfuAI	ACCTGC	3 cut(s)	721, 735, 1232
BglII	AGATCT	1 cut(s)	717
BisI	GCNGC	6 cut(s)	15, 248, 699, 951, 1221, 2202
BlpI	GCTNAGC	1 cut(s)	1461
BlsI	GCNGC	6 cut(s)	16, 249, 700, 952, 1222, 2203
Bme1390I	CCNGG	3 cut(s)	204, 975, 1336
Bme18I	GGWCC	4 cut(s)	206, 1045, 1373, 1814
BmeT110I	CYCGRG	1 cut(s)	500
BmgT120I	GGNCC	6 cut(s)	206, 1045, 1373, 1597, 1598, 1814
BmiI	GGNNCC	3 cut(s)	1599, 2086, 2283
BmrFI	CCNGG	3 cut(s)	204, 975, 1336
BmsI	GCATC	8 cut(s)	286, 652, 772, 794, 1048, 1285, 1384, 1991
BplI	GAGNNNNNCTC	2 cut(s)	1334, 1366
BpmI	CTGGAG	3 cut(s)	141, 431, 978
Bpu1102I	GCTNAGC	1 cut(s)	1461
BpuMI	CCSGG	2 cut(s)	204, 975
BsaAI	YACGTR	1 cut(s)	508
BsaBI	GATNNNNATC	2 cut(s)	35, 53
BsaJI	CCNNGG	3 cut(s)	836, 974, 1533
BsaWI	WCCGGW	2 cut(s)	2284, 2308
Bsc4I	CCNNNNNNNGG	6 cut(s)	60, 146, 412, 923, 1153, 2161
Bse118I	RCCGGY	3 cut(s)	139, 178, 2284
Bse1I	ACTGG	5 cut(s)	158, 928, 1254, 1518, 2072
Bse3DI	GCAATG	1 cut(s)	2054
Bse8I	GATNNNNATC	2 cut(s)	35, 53
BseBI	CCWGG	1 cut(s)	1336
BseDI	CCNNGG	3 cut(s)	836, 974, 1533
BseGI	GGATG	6 cut(s)	718, 816, 1344, 1366, 1450, 2006
BseJI	GATNNNNATC	2 cut(s)	35, 53
BseLI	CCNNNNNNNGG	6 cut(s)	60, 146, 412, 923, 1153, 2161
BseMI	GCAATG	1 cut(s)	2054
BseMII	CTCAG	8 cut(s)	897, 1338, 1475, 1698, 1896, 2025, 2147, 2307
BseNI	ACTGG	5 cut(s)	158, 928, 1254, 1518, 2072
BseRI	GAGGAG	2 cut(s)	284, 482
BseSI	GKGCMC	2 cut(s)	1601, 1927
BseXI	GCAGC	5 cut(s)	234, 685, 937, 1207, 2188
BsgI	GTGCAG	1 cut(s)	2212
BshFI	GGCC	3 cut(s)	1599, 1691, 1917
BshNI	GGYRCC	1 cut(s)	2281
BshTI	ACCGGT	1 cut(s)	2284
BsiHKCI	CYCGRG	1 cut(s)	500
BsiSI	CCGG	7 cut(s)	140, 179, 204, 974, 1584, 2285, 2309
BslFI	GGGAC	2 cut(s)	163, 948
BslI	CCNNNNNNNGG	6 cut(s)	60, 146, 412, 923, 1153, 2161
BsmAI	GTCTC	3 cut(s)	35, 247, 445
BsmBI	CGTCTC	1 cut(s)	35
BsmFI	GGGAC	2 cut(s)	163, 948
BsmI	GAATGC	1 cut(s)	1802
BsnI	GGCC	3 cut(s)	1599, 1691, 1917
BsoBI	CYCGRG	1 cut(s)	500
Bsp120I	GGGCCC	1 cut(s)	1597
Bsp1286I	GDGCHC	3 cut(s)	657, 1601, 1927
Bsp1407I	TGTACA	3 cut(s)	889, 1173, 2073
Bsp143I	GATC	5 cut(s)	309, 397, 496, 717, 1648
Bsp1720I	GCTNAGC	1 cut(s)	1461
Bsp19I	CCATGG	1 cut(s)	836
BspACI	CCGC	4 cut(s)	61, 176, 2008, 2144
BspANI	GGCC	3 cut(s)	1599, 1691, 1917
BspCNI	CTCAG	8 cut(s)	896, 1337, 1474, 1697, 1895, 2024, 2148, 2308
BspLI	GGNNCC	3 cut(s)	1599, 2086, 2283
BspMAI	CTGCAG	2 cut(s)	732, 880
BspMI	ACCTGC	3 cut(s)	721, 735, 1232
BspPI	GGATC	2 cut(s)	392, 491
BspQI	GCTCTTC	1 cut(s)	1955
BspT107I	GGYRCC	1 cut(s)	2281
BspTI	CTTAAG	2 cut(s)	1673, 2027
BsrBI	CCGCTC	2 cut(s)	61, 2010
BsrDI	GCAATG	1 cut(s)	2054
BsrFI	RCCGGY	3 cut(s)	139, 178, 2284
BsrGI	TGTACA	3 cut(s)	889, 1173, 2073
BsrI	ACTGG	5 cut(s)	158, 928, 1254, 1518, 2072
BssAI	RCCGGY	3 cut(s)	139, 178, 2284
BssECI	CCNNGG	3 cut(s)	836, 974, 1533
BssMI	GATC	5 cut(s)	309, 397, 496, 717, 1648
BssNAI	GTATAC	1 cut(s)	379
BssT1I	CCWWGG	2 cut(s)	836, 1533
Bst1107I	GTATAC	1 cut(s)	379
Bst2UI	CCWGG	1 cut(s)	1336
Bst4CI	ACNGT	7 cut(s)	743, 894, 1042, 1094, 1401, 1609, 1855
Bst6I	CTCTTC	5 cut(s)	261, 429, 1022, 1265, 1955
BstAFI	CTTAAG	2 cut(s)	1673, 2027
BstAPI	GCANNNNNTGC	1 cut(s)	99
BstAUI	TGTACA	3 cut(s)	889, 1173, 2073
BstBAI	YACGTR	1 cut(s)	508
BstC8I	GCNNGC	3 cut(s)	91, 141, 777
BstDSI	CCRYGG	1 cut(s)	836
BstF5I	GGATG	6 cut(s)	718, 816, 1344, 1366, 1450, 2006
BstHHI	GCGC	1 cut(s)	1638
BstKTI	GATC	5 cut(s)	312, 400, 499, 720, 1651
BstMAI	GTCTC	3 cut(s)	35, 247, 445
BstMBI	GATC	5 cut(s)	309, 397, 496, 717, 1648
BstMWI	GCNNNNNNNGC	3 cut(s)	99, 884, 1797
BstNI	CCWGG	1 cut(s)	1336
BstNSI	RCATGY	1 cut(s)	779
BstSCI	CCNGG	3 cut(s)	202, 973, 1334
BstSFI	CTRYAG	6 cut(s)	579, 728, 876, 1368, 1716, 1851
BstSLI	GKGCMC	2 cut(s)	1601, 1927
BstSNI	TACGTA	1 cut(s)	508
BstV1I	GCAGC	5 cut(s)	234, 685, 937, 1207, 2188
BstX2I	RGATCY	3 cut(s)	397, 496, 717
BstYI	RGATCY	3 cut(s)	397, 496, 717
BstZ17I	GTATAC	1 cut(s)	379
BsuRI	GGCC	3 cut(s)	1599, 1691, 1917
BtgI	CCRYGG	1 cut(s)	836
BtgZI	GCGATG	1 cut(s)	1308
BtsCI	GGATG	6 cut(s)	718, 816, 1344, 1366, 1450, 2006
BtsI	GCAGTG	3 cut(s)	197, 596, 2224
BveI	ACCTGC	3 cut(s)	721, 735, 1232
Cac8I	GCNNGC	3 cut(s)	91, 141, 777
CfoI	GCGC	1 cut(s)	1638
Cfr10I	RCCGGY	3 cut(s)	139, 178, 2284
Cfr13I	GGNCC	6 cut(s)	206, 1045, 1373, 1597, 1598, 1814
Csp6I	GTAC	8 cut(s)	505, 509, 565, 739, 890, 1174, 2074, 2282
CspAI	ACCGGT	1 cut(s)	2284
CviQI	GTAC	8 cut(s)	505, 509, 565, 739, 890, 1174, 2074, 2282
DpnI	GATC	5 cut(s)	311, 399, 498, 719, 1650
DpnII	GATC	5 cut(s)	309, 397, 496, 717, 1648
DraI	TTTAAA	1 cut(s)	1119
Eam1104I	CTCTTC	5 cut(s)	261, 429, 1022, 1265, 1955
EarI	CTCTTC	5 cut(s)	261, 429, 1022, 1265, 1955
EciI	GGCGGA	1 cut(s)	165
Eco105I	TACGTA	1 cut(s)	508
Eco130I	CCWWGG	2 cut(s)	836, 1533
Eco24I	GRGCYC	2 cut(s)	657, 1601
Eco47I	GGWCC	4 cut(s)	206, 1045, 1373, 1814
Eco57I	CTGAAG	3 cut(s)	410, 924, 2201
Eco88I	CYCGRG	1 cut(s)	500
EcoO109I	RGGNCCY	2 cut(s)	1597, 1598
EcoRI	GAATTC	1 cut(s)	365
EcoRII	CCWGG	1 cut(s)	1334
EcoT14I	CCWWGG	2 cut(s)	836, 1533
EcoT22I	ATGCAT	2 cut(s)	301, 787
EcoT38I	GRGCYC	2 cut(s)	657, 1601
ErhI	CCWWGG	2 cut(s)	836, 1533
Esp3I	CGTCTC	1 cut(s)	35
FalI	AAGNNNNNCTT	2 cut(s)	86, 118
FaqI	GGGAC	2 cut(s)	163, 948
FauNDI	CATATG	1 cut(s)	1387
FblI	GTMKAC	3 cut(s)	378, 1524, 1850
Fnu4HI	GCNGC	6 cut(s)	15, 248, 699, 951, 1221, 2202
FokI	GGATG	6 cut(s)	725, 823, 1351, 1373, 1457, 2013
FriOI	GRGCYC	2 cut(s)	657, 1601
Fsp4HI	GCNGC	6 cut(s)	15, 248, 699, 951, 1221, 2202
FspBI	CTAG	6 cut(s)	275, 395, 485, 1101, 1106, 1263
GlaI	GCGC	1 cut(s)	1637
GluI	GCNGC	6 cut(s)	15, 248, 699, 951, 1221, 2202
GsuI	CTGGAG	3 cut(s)	141, 431, 978
HaeIII	GGCC	3 cut(s)	1599, 1691, 1917
HapII	CCGG	7 cut(s)	140, 179, 204, 974, 1584, 2285, 2309
HhaI	GCGC	1 cut(s)	1638
Hin6I	GCGC	1 cut(s)	1636
HinP1I	GCGC	1 cut(s)	1636
HincII	GTYRAC	2 cut(s)	648, 2122
HindII	GTYRAC	2 cut(s)	648, 2122
HindIII	AAGCTT	1 cut(s)	478
HpaII	CCGG	7 cut(s)	140, 179, 204, 974, 1584, 2285, 2309
HphI	GGTGA	6 cut(s)	193, 716, 1251, 1256, 1490, 2201
Hpy166II	GTNNAC	8 cut(s)	379, 648, 1045, 1176, 1259, 1525, 1851, 2122
Hpy188I	TCNGA	3 cut(s)	1327, 1687, 2220
Hpy8I	GTNNAC	8 cut(s)	379, 648, 1045, 1176, 1259, 1525, 1851, 2122
Hpy99I	CGWCG	1 cut(s)	192
HpyAV	CCTTC	4 cut(s)	763, 925, 1780, 2132
HpyCH4III	ACNGT	7 cut(s)	743, 894, 1042, 1094, 1401, 1609, 1855
HpyCH4IV	ACGT	3 cut(s)	114, 507, 616
HpyF10VI	GCNNNNNNNGC	3 cut(s)	99, 884, 1797
HpySE526I	ACGT	3 cut(s)	114, 507, 616
HspAI	GCGC	1 cut(s)	1636
KpnI	GGTACC	1 cut(s)	2285
KroI	GCCGGC	1 cut(s)	139
KroNI	GCCGGC	1 cut(s)	141
Kzo9I	GATC	5 cut(s)	309, 397, 496, 717, 1648
LguI	GCTCTTC	1 cut(s)	1955
LmnI	GCTCC	8 cut(s)	259, 271, 628, 772, 1498, 1555, 1959, 2084
Lsp1109I	GCAGC	5 cut(s)	234, 685, 937, 1207, 2188
LweI	GCATC	8 cut(s)	286, 652, 772, 794, 1048, 1285, 1384, 1991
MaeI	CTAG	6 cut(s)	275, 395, 485, 1101, 1106, 1263
MaeII	ACGT	3 cut(s)	114, 507, 616
MaeIII	GTNAC	7 cut(s)	385, 594, 628, 919, 1244, 1363, 2189
MalI	GATC	5 cut(s)	311, 399, 498, 719, 1650
MbiI	CCGCTC	2 cut(s)	61, 2010
MboI	GATC	5 cut(s)	309, 397, 496, 717, 1648
MfeI	CAATTG	1 cut(s)	539
MflI	RGATCY	3 cut(s)	397, 496, 717
MhlI	GDGCHC	3 cut(s)	657, 1601, 1927
MlyI	GAGTC	5 cut(s)	841, 875, 1156, 1316, 1343
MmeI	TCCRAC	4 cut(s)	233, 637, 908, 1326
Mph1103I	ATGCAT	2 cut(s)	301, 787
MroNI	GCCGGC	1 cut(s)	139
MroXI	GAANNNNTTC	1 cut(s)	2274
MslI	CAYNNNNRTG	2 cut(s)	780, 841
MspA1I	CMGCKG	1 cut(s)	887
MspCI	CTTAAG	2 cut(s)	1673, 2027
MspI	CCGG	7 cut(s)	140, 179, 204, 974, 1584, 2285, 2309
MspR9I	CCNGG	3 cut(s)	204, 975, 1336
MunI	CAATTG	1 cut(s)	539
Mva1269I	GAATGC	1 cut(s)	1802
MvaI	CCWGG	1 cut(s)	1336
MwoI	GCNNNNNNNGC	3 cut(s)	99, 884, 1797
NaeI	GCCGGC	1 cut(s)	141
NciI	CCSGG	2 cut(s)	204, 975
NcoI	CCATGG	1 cut(s)	836
NdeI	CATATG	1 cut(s)	1387
NdeII	GATC	5 cut(s)	309, 397, 496, 717, 1648
NgoMIV	GCCGGC	1 cut(s)	139
NlaIV	GGNNCC	3 cut(s)	1599, 2086, 2283
NmuCI	GTSAC	4 cut(s)	594, 1244, 1363, 2189
NsiI	ATGCAT	2 cut(s)	301, 787
NspI	RCATGY	1 cut(s)	779
PacI	TTAATTAA	1 cut(s)	825
PaeI	GCATGC	1 cut(s)	779
PaqCI	CACCTGC	3 cut(s)	721, 735, 1232
PciSI	GCTCTTC	1 cut(s)	1955
PctI	GAATGC	1 cut(s)	1802
PdiI	GCCGGC	1 cut(s)	141
PdmI	GAANNNNTTC	1 cut(s)	2274
PfeI	GAWTC	7 cut(s)	130, 184, 1097, 1109, 1766, 2098, 2329
PflFI	GACNNNGTC	1 cut(s)	1520
PflMI	CCANNNNNTGG	1 cut(s)	1153
PinAI	ACCGGT	1 cut(s)	2284
PkrI	GCNGC	6 cut(s)	16, 249, 700, 952, 1222, 2203
PleI	GAGTC	5 cut(s)	840, 875, 1156, 1316, 1343
PpsI	GAGTC	5 cut(s)	840, 875, 1156, 1316, 1343
Ppu21I	YACGTR	1 cut(s)	508
PshBI	ATTAAT	1 cut(s)	825
Psp1406I	AACGTT	1 cut(s)	616
Psp6I	CCWGG	1 cut(s)	1334
PspGI	CCWGG	1 cut(s)	1334
PspN4I	GGNNCC	3 cut(s)	1599, 2086, 2283
PspOMI	GGGCCC	1 cut(s)	1597
PspPI	GGNCC	6 cut(s)	206, 1045, 1373, 1597, 1598, 1814
PstI	CTGCAG	2 cut(s)	732, 880
PsuI	RGATCY	3 cut(s)	397, 496, 717
PsyI	GACNNNGTC	1 cut(s)	1520
PvuII	CAGCTG	1 cut(s)	887
RsaI	GTAC	8 cut(s)	506, 510, 566, 740, 891, 1175, 2075, 2283
RsaNI	GTAC	8 cut(s)	505, 509, 565, 739, 890, 1174, 2074, 2282
RseI	CAYNNNNRTG	2 cut(s)	780, 841
SapI	GCTCTTC	1 cut(s)	1955
SatI	GCNGC	6 cut(s)	15, 248, 699, 951, 1221, 2202
Sau3AI	GATC	5 cut(s)	309, 397, 496, 717, 1648
Sau96I	GGNCC	6 cut(s)	206, 1045, 1373, 1597, 1598, 1814
SbfI	CCTGCAGG	1 cut(s)	732
SchI	GAGTC	5 cut(s)	841, 875, 1156, 1316, 1343
ScrFI	CCNGG	3 cut(s)	204, 975, 1336
SdaI	CCTGCAGG	1 cut(s)	732
SduI	GDGCHC	3 cut(s)	657, 1601, 1927
SfaNI	GCATC	8 cut(s)	286, 652, 772, 794, 1048, 1285, 1384, 1991
SfcI	CTRYAG	6 cut(s)	579, 728, 876, 1368, 1716, 1851
SgrAI	CRCCGGYG	1 cut(s)	178
SinI	GGWCC	4 cut(s)	206, 1045, 1373, 1814
SmiMI	CAYNNNNRTG	2 cut(s)	780, 841
SmlI	CTYRAG	2 cut(s)	1673, 2027
SmoI	CTYRAG	2 cut(s)	1673, 2027
SnaBI	TACGTA	1 cut(s)	508
SphI	GCATGC	1 cut(s)	779
Sse8387I	CCTGCAGG	1 cut(s)	732
SsiI	CCGC	4 cut(s)	61, 176, 2008, 2144
SspMI	CTAG	6 cut(s)	275, 395, 485, 1101, 1106, 1263
StyD4I	CCNGG	3 cut(s)	202, 973, 1334
StyI	CCWWGG	2 cut(s)	836, 1533
TaaI	ACNGT	7 cut(s)	743, 894, 1042, 1094, 1401, 1609, 1855
TaiI	ACGT	3 cut(s)	117, 510, 619
TaqI	TCGA	7 cut(s)	39, 57, 586, 1160, 1558, 1566, 1651
TaqII	GACCGA	1 cut(s)	2012
TatI	WGTACW	3 cut(s)	889, 1173, 2073
TfiI	GAWTC	7 cut(s)	130, 184, 1097, 1109, 1766, 2098, 2329
TseFI	GTSAC	4 cut(s)	594, 1244, 1363, 2189
TseI	GCWGC	6 cut(s)	14, 247, 698, 950, 1220, 2201
Tsp45I	GTSAC	4 cut(s)	594, 1244, 1363, 2189
TspDTI	ATGAA	8 cut(s)	17, 55, 343, 378, 867, 1404, 1809, 2121
TspGWI	ACGGA	2 cut(s)	574, 1025
Tth111I	GACNNNGTC	1 cut(s)	1520
Van91I	CCANNNNNTGG	1 cut(s)	1153
Vha464I	CTTAAG	2 cut(s)	1673, 2027
VpaK11BI	GGWCC	4 cut(s)	206, 1045, 1373, 1814
VspI	ATTAAT	1 cut(s)	825
XapI	RAATTY	5 cut(s)	350, 365, 1013, 1019, 1931
XbaI	TCTAGA	2 cut(s)	394, 1105
XceI	RCATGY	1 cut(s)	779
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2308
XmiI	GTMKAC	3 cut(s)	378, 1524, 1850
XmnI	GAANNNNTTC	1 cut(s)	2274
XspI	CTAG	6 cut(s)	275, 395, 485, 1101, 1106, 1263
Zsp2I	ATGCAT	2 cut(s)	301, 787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.