Rroxscaffold_3G00237150

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003807
Physical Location & Seq
Forward (+)
24736840 .. 24743132
6293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237150.1

Sequence Viewer

Length: 1218 bp
ATGGCTTTTCGTGGCGGTGCAACGCGTTCCCTTGGTTCAATGCTGTGTAGTTATAGATCGTCAACTACAGCTACTGCTGCTAATGGCCGAGGTTTTTCTACCTGTGTCTCAAACAAAAGAGCTGTTTTTTCTCAAGTCACAGAGACCCAAACCTCGATTCCTTTGAGACTTGCCAATCCCCAATGTGGGTCTGCTCTCTTTCTTCTTAGGACGTTGAGCGTGGGAGCATCGTCGTCTCCTGACCAAATGAGTCTTATAAAGCAACTCAGGCAGAGAACCAGTGCTCCTATCAAAGATGTCAAGGCTGCTCTCATTGATTGCAATTGGGAAATCGAGGCTGCACTGAAGGAATTAAGGAGAAGAGGGAAGGTTTTGGCATCAAAAAAGTCATCAAGAATGGCTTCTGAGGGTTTGCTTGCACTGGCTCAGAACGAGAGCAGGGCTGCCATTATCGAACTCAACTGTGAGACTGACTTTGTTGCTAGAAATGAAATCTTTCAATACTTGGCATCAGCTTTGGCAAAGCAAGCTTTGCTGGTTGAAGGCTCTTCTCAACAGGTTCCTGGGGTCATTCCCATTGCCTCGGAACATTTAGAGGACTTGAAGTTAAATCTTGAACATCCAAAAATTAGTGGAGAAACAACTGTTCAGAATGCCATGACGGAAGTGGCTGCAGTAATGGGGGAAAATATTAAACTTAGAAGGGGATTTGTGATGCCTACAGCTTCACATGGTGTTGTTTCCACGTATCTCCATGCAAGCCCCCAACCAGGTTTGGGTCGCATTGCTGGAATTTTATCTCTCAAAGTAGAGGACAAGAGTGCTCCGTTGGATGCCATCCAGCGCGTTGGGTCAGAATTGGCAATGCATGTGGTGGCTGCAAAGCCTTTATTTTTAACAAAGGAACATGTTTCTTCTGATGCATTAGAGAGTGAACGGGAAATTCTCAAGTCTCAGGCTGAAAGTACGGGCAAGTCTCAGATGGCCATAGACAAAATAGTAGAAGGTCGTTTGCGTAAATACTACGAGGAAGTTGTTCTTATGGAGCAGAAGTTTATTGTGGACGATTCTGTAAATGTAAAGACACTATTAGATAAGCTATCTAAGGAAGTGGGATCACCTGTGAAGATTGAGAGCTTTTTCAGGATGGAAGTTGGAGAAGGAATTCAAAGGCTTGAAGCATCCAATACATCTGAAAATTTGGCTGAGGCCGCTTGA

Protein Analysis

405

Amino Acids

43.88

Weight (kDa)

8.48

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF_TS PF00889 148 - 387 1.5e-55 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccII CGCG 2 cut(s) 25, 846
AciI CCGC 2 cut(s) 15, 1212
AclWI GGATC 1 cut(s) 1123
AcoI YGGCCR 2 cut(s) 85, 984
AcsI RAATTY 4 cut(s) 792, 942, 1164, 1198
AcuI CTGAAG 1 cut(s) 365
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 1 cut(s) 967
AfiI CCNNNNNNNGG 4 cut(s) 185, 186, 770, 776
AflIII ACRYGT 2 cut(s) 23, 907
AgsI TTSAA 7 cut(s) 39, 500, 542, 604, 617, 1169, 1178
AjnI CCWGG 2 cut(s) 562, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 7 cut(s) 71, 122, 515, 530, 725, 1099, 1137
AluI AGCT 7 cut(s) 71, 122, 515, 530, 725, 1099, 1137
Alw21I GWGCWC 2 cut(s) 286, 826
Alw26I GTCTC 7 cut(s) 112, 137, 160, 240, 461, 957, 981
AlwI GGATC 1 cut(s) 1123
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 3 cut(s) 85, 984, 1209
ApeKI GCWGC 6 cut(s) 77, 305, 338, 443, 671, 878
ApoI RAATTY 4 cut(s) 792, 942, 1164, 1198
Asp700I GAANNNNTTC 4 cut(s) 400, 495, 1035, 1164
AspLEI GCGC 1 cut(s) 846
AsuHPI GGTGA 1 cut(s) 1110
BalI TGGCCA 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 286, 826
BbvCI CCTCAGC 1 cut(s) 1206
BbvI GCAGC 6 cut(s) 64, 292, 325, 430, 658, 865
BccI CCATC 3 cut(s) 845, 976, 1141
BciT130I CCWGG 2 cut(s) 564, 771
BcoDI GTCTC 7 cut(s) 112, 137, 160, 240, 461, 957, 981
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 3 cut(s) 66, 672, 720
BisI GCNGC 7 cut(s) 78, 306, 339, 444, 672, 879, 1212
BlsI GCNGC 7 cut(s) 79, 307, 340, 445, 673, 880, 1213
Bme1390I CCNGG 2 cut(s) 564, 771
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 2 cut(s) 564, 771
BmsI GCATC 7 cut(s) 236, 386, 518, 705, 823, 910, 1190
Bpu10I CCTNAGC 1 cut(s) 1206
BpuEI CTTGAG 2 cut(s) 117, 932
BsaAI YACGTR 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 4 cut(s) 31, 88, 563, 582
BsaXI ACNNNNNCTCC 4 cut(s) 268, 298, 1037, 1067
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 186, 770, 776
Bse1I ACTGG 2 cut(s) 279, 426
Bse3DI GCAATG 3 cut(s) 576, 783, 870
BseBI CCWGG 2 cut(s) 564, 771
BseDI CCNNGG 4 cut(s) 31, 88, 563, 582
BseGI GGATG 5 cut(s) 619, 837, 838, 1152, 1181
BseLI CCNNNNNNNGG 4 cut(s) 185, 186, 770, 776
BseMI GCAATG 3 cut(s) 576, 783, 870
BseMII CTCAG 6 cut(s) 280, 396, 440, 968, 992, 1197
BseNI ACTGG 2 cut(s) 279, 426
BseXI GCAGC 6 cut(s) 64, 292, 325, 430, 658, 865
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 25, 846
BshFI GGCC 3 cut(s) 87, 986, 1211
BsiHKAI GWGCWC 2 cut(s) 286, 826
BslI CCNNNNNNNGG 4 cut(s) 185, 186, 770, 776
BsmAI GTCTC 7 cut(s) 112, 137, 160, 240, 461, 957, 981
BsmBI CGTCTC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 3 cut(s) 87, 986, 1211
Bso31I GGTCTC 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 286, 826
Bsp143I GATC 2 cut(s) 56, 1115
BspACI CCGC 2 cut(s) 15, 1212
BspANI GGCC 3 cut(s) 87, 986, 1211
BspCNI CTCAG 6 cut(s) 279, 397, 439, 967, 991, 1198
BspFNI CGCG 2 cut(s) 25, 846
BspLI GGNNCC 1 cut(s) 561
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 1 cut(s) 1123
BspQI GCTCTTC 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 137
BsrDI GCAATG 3 cut(s) 576, 783, 870
BsrI ACTGG 2 cut(s) 279, 426
BssECI CCNNGG 4 cut(s) 31, 88, 563, 582
BssMI GATC 2 cut(s) 56, 1115
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 564, 771
Bst4CI ACNGT 2 cut(s) 464, 646
Bst6I CTCTTC 2 cut(s) 355, 553
BstAPI GCANNNNNTGC 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 747
BstC8I GCNNGC 3 cut(s) 417, 528, 760
BstDEI CTNAG 9 cut(s) 206, 266, 405, 426, 698, 954, 978, 1104, 1206
BstF5I GGATG 5 cut(s) 619, 837, 838, 1152, 1181
BstFNI CGCG 2 cut(s) 25, 846
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 2 cut(s) 59, 1118
BstMAI GTCTC 7 cut(s) 112, 137, 160, 240, 461, 957, 981
BstMBI GATC 2 cut(s) 56, 1115
BstMWI GCNNNNNNNGC 5 cut(s) 77, 268, 527, 532, 1211
BstNI CCWGG 2 cut(s) 564, 771
BstNSI RCATGY 2 cut(s) 872, 911
BstSCI CCNGG 2 cut(s) 562, 769
BstSFI CTRYAG 3 cut(s) 66, 672, 720
BstUI CGCG 2 cut(s) 25, 846
BstV1I GCAGC 6 cut(s) 64, 292, 325, 430, 658, 865
BstXI CCANNNNNNTGG 1 cut(s) 848
BsuRI GGCC 3 cut(s) 87, 986, 1211
BtsCI GGATG 5 cut(s) 619, 837, 838, 1152, 1181
BtsIMutI CAGTG 3 cut(s) 286, 341, 419
Cac8I GCNNGC 3 cut(s) 417, 528, 760
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 846
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 1 cut(s) 966
CspCI CAANNNNNGTGG 2 cut(s) 852, 887
CviAII CATG 5 cut(s) 658, 731, 755, 869, 908
CviQI GTAC 1 cut(s) 966
DdeI CTNAG 9 cut(s) 206, 266, 405, 426, 698, 954, 978, 1104, 1206
DpnI GATC 2 cut(s) 58, 1117
DpnII GATC 2 cut(s) 56, 1115
DraIII CACNNNGTG 1 cut(s) 734
EaeI YGGCCR 2 cut(s) 85, 984
Eam1104I CTCTTC 2 cut(s) 355, 553
EarI CTCTTC 2 cut(s) 355, 553
Eco130I CCWWGG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 1164
EcoRII CCWGG 2 cut(s) 562, 769
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 2 cut(s) 870, 925
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 5 cut(s) 661, 734, 758, 872, 911
FaiI YATR 9 cut(s) 54, 257, 659, 732, 756, 870, 909, 989, 1043
FalI AAGNNNNNCTT 4 cut(s) 385, 417, 1023, 1055
FatI CATG 5 cut(s) 657, 730, 754, 868, 907
Fnu4HI GCNGC 7 cut(s) 78, 306, 339, 444, 672, 879, 1212
FokI GGATG 5 cut(s) 606, 824, 845, 1159, 1168
Fsp4HI GCNGC 7 cut(s) 78, 306, 339, 444, 672, 879, 1212
FspBI CTAG 1 cut(s) 483
GlaI GCGC 1 cut(s) 845
GluI GCNGC 7 cut(s) 78, 306, 339, 444, 672, 879, 1212
HaeIII GGCC 3 cut(s) 87, 986, 1211
HhaI GCGC 1 cut(s) 846
Hin1II CATG 5 cut(s) 661, 734, 758, 872, 911
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 3 cut(s) 157, 250, 1067
HphI GGTGA 1 cut(s) 1110
Hpy166II GTNNAC 3 cut(s) 63, 935, 1063
Hpy188I TCNGA 8 cut(s) 406, 429, 586, 651, 856, 919, 981, 1195
Hpy188III TCNNGA 4 cut(s) 239, 393, 614, 1144
Hpy8I GTNNAC 3 cut(s) 63, 935, 1063
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 6 cut(s) 340, 361, 536, 696, 998, 1154
HpyCH4III ACNGT 2 cut(s) 464, 646
HpyCH4IV ACGT 2 cut(s) 212, 746
HpyCH4V TGCA 9 cut(s) 20, 321, 341, 419, 674, 758, 868, 881, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 268, 527, 532, 1211
HpyF3I CTNAG 9 cut(s) 206, 266, 405, 426, 698, 954, 978, 1104, 1206
HpySE526I ACGT 2 cut(s) 212, 746
Hsp92II CATG 5 cut(s) 661, 734, 758, 872, 911
HspAI GCGC 1 cut(s) 844
Kzo9I GATC 2 cut(s) 56, 1115
LguI GCTCTTC 1 cut(s) 553
LmnI GCTCC 4 cut(s) 224, 289, 829, 1045
Lsp1109I GCAGC 6 cut(s) 64, 292, 325, 430, 658, 865
LweI GCATC 7 cut(s) 236, 386, 518, 705, 823, 910, 1190
MabI ACCWGGT 1 cut(s) 769
MaeI CTAG 1 cut(s) 483
MaeII ACGT 2 cut(s) 212, 746
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 58, 1117
MboI GATC 2 cut(s) 56, 1115
MboII GAAGA 5 cut(s) 194, 372, 540, 906, 1138
MfeI CAATTG 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 286, 826
MlsI TGGCCA 1 cut(s) 986
MluCI AATT 8 cut(s) 322, 350, 627, 792, 857, 942, 1164, 1198
MluI ACGCGT 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 986
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 2 cut(s) 810, 1135
Mox20I TGGCCA 1 cut(s) 986
Mph1103I ATGCAT 2 cut(s) 870, 925
MroXI GAANNNNTTC 4 cut(s) 400, 495, 1035, 1164
MscI TGGCCA 1 cut(s) 986
MseI TTAA 4 cut(s) 353, 608, 693, 896
Msp20I TGGCCA 1 cut(s) 986
MspR9I CCNGG 2 cut(s) 564, 771
MunI CAATTG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 2 cut(s) 564, 771
MvnI CGCG 2 cut(s) 25, 846
MwoI GCNNNNNNNGC 5 cut(s) 77, 268, 527, 532, 1211
NdeII GATC 2 cut(s) 56, 1115
NlaIII CATG 5 cut(s) 661, 734, 758, 872, 911
NlaIV GGNNCC 1 cut(s) 561
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 2 cut(s) 870, 925
NspI RCATGY 2 cut(s) 872, 911
PciI ACATGT 1 cut(s) 907
PciSI GCTCTTC 1 cut(s) 553
PctI GAATGC 1 cut(s) 658
PdmI GAANNNNTTC 4 cut(s) 400, 495, 1035, 1164
PfeI GAWTC 2 cut(s) 157, 1067
PkrI GCNGC 7 cut(s) 79, 307, 340, 445, 673, 880, 1213
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
Ppu21I YACGTR 1 cut(s) 747
PscI ACATGT 1 cut(s) 907
PsiI TTATAA 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 562, 769
PspGI CCWGG 2 cut(s) 562, 769
PspN4I GGNNCC 1 cut(s) 561
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 967
RsaNI GTAC 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 4 cut(s) 353, 608, 693, 896
SatI GCNGC 7 cut(s) 78, 306, 339, 444, 672, 879, 1212
Sau3AI GATC 2 cut(s) 56, 1115
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 564, 771
SduI GDGCHC 2 cut(s) 286, 826
SexAI ACCWGGT 1 cut(s) 769
SfaNI GCATC 7 cut(s) 236, 386, 518, 705, 823, 910, 1190
SfcI CTRYAG 3 cut(s) 66, 672, 720
SmlI CTYRAG 2 cut(s) 132, 947
SmoI CTYRAG 2 cut(s) 132, 947
Sse9I AATT 8 cut(s) 322, 350, 627, 792, 857, 942, 1164, 1198
SsiI CCGC 2 cut(s) 15, 1212
SspI AATATT 1 cut(s) 691
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 562, 769
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 464, 646
TaiI ACGT 2 cut(s) 215, 749
TaqI TCGA 3 cut(s) 155, 333, 453
TasI AATT 8 cut(s) 322, 350, 627, 792, 857, 942, 1164, 1198
TauI GCSGC 1 cut(s) 1214
TfiI GAWTC 2 cut(s) 157, 1067
Tru1I TTAA 4 cut(s) 353, 608, 693, 896
Tru9I TTAA 4 cut(s) 353, 608, 693, 896
TscAI CASTG 3 cut(s) 286, 348, 426
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 6 cut(s) 77, 305, 338, 443, 671, 878
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 504
TspGWI ACGGA 2 cut(s) 677, 816
TspRI CASTG 3 cut(s) 286, 348, 426
XapI RAATTY 4 cut(s) 792, 942, 1164, 1198
XceI RCATGY 2 cut(s) 872, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XmnI GAANNNNTTC 4 cut(s) 400, 495, 1035, 1164
XspI CTAG 1 cut(s) 483
Zsp2I ATGCAT 2 cut(s) 870, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.