Rroxscaffold_3G00275640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011234
Physical Location & Seq
Reverse (-)
67025226 .. 67029245
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275640.1

Sequence Viewer

Length: 1074 bp
ATGTCCGCTGCCTCATCAGCTCTGACACTCCGCCTCCCCTTCGCCTCCTCAATCACCACCACCACCACCACCACCGCCACTAAGCTCATTTATCCGATATGCAGCTCACGTCGCTCTATAAGCAGTTTGAGGCTTGTGGGTTGCGCCCGTGCTGCCATTTCAAGCCAAGCGCAAGGTACATTTGATCCTGAACTCCGTTTAGTACTTGAACTTGCCACTGATTCTGAGTTATATGAGATTGAAAGAATCCTTTTTGGCCCTAGCTACTTCAGCCCTTTGCTGAAATCCATAATGAGCAGAACCAACGTCGACTATGCAATGATTGAAGAGGATCTTGAGGAGCGAGATCAGTTTTTATCAGCATTAGAGTCGCGATTCTTATTCCTCGCAGCGGATGCTCGGTCCACAATAAGGGGTTGGAGGCCATCATATAGGAATGTCTTGCTTGCAGTTAGAAAAGAGTTGAACATTCCTTGCTCAAGCAAATTATCAACTGAAGACCTTGAAGCAGAGATTTTCCTTCATCTGTTGCGAGACTACTCAAGGCATGTTGAAGAATCAGGAAGTTTCGAGGGCTTGCTAGAATTCTCTGAGCCGTCAGATGCTGAAAACAGTCTTGAACTTGGACTCAGTCAATGGAAGGTGCAGGCCCTCACTGCTTTAAAAGTTGGGGCAGCGGAACTCCGATCAATGTTTTTAAAGGGTGGCGGTATCCTTACTCTGGCAAAGATCTATCAATTGTTAGCTAGGAAGTTATCTGGGAAGGTGTTCTTAGAAGCTGCCAACTATCAACTGAAAAGGGATGTTGTTAGAAAGGGTGGACAATTAGCAGCTATTAATCTAGAATCCAGAGTGGCCTTGCTAGCTGCAAAACAAGGTTTTGCAGGGGCTACATCAAGATATCTTGGCTTGAGAAGCATGATGTCGTTACTTGGCCCAGTGCTTTGGGGAACATTTCTGGCGGATGTAGTCATTCAAATGCTAGGAACCGATTATGCAAGAATTTTGCGAGCAATTTATGCTTTTGCACAGATACGCATCACTCGCACTTATGGGTCACCGGTTGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

39.46

Weight (kDa)

8.66

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73470 AT1G73470 AT1G73470
fragaria_vesca FvH4_5g17910 FvH4_5g17910
malus_domestica MD06G1234700.v1.1 MD14G1241900.v1.1
prunus_persica Prupe.5G239500_v2.0.a1 Prupe.5G239500_v2.0.a1
pyrus_communis pycom06g20940
rosa_chinensis RchiOBHm_Chr7g0177701
rosa_laevigata RLG00000005513
rosa_multiflora Rmu_sc0004786.1_g000002
rosa_roxburghii Rroxscaffold_3G00275640
rosa_rugosa Rorug06G0408400
rosa_samantha Rh7AG008100 Rh7BG008100 Rh7CG008500 Rh7DG008200
rosa_wichuraiana Rw7G000680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 373
AciI CCGC 7 cut(s) 6, 31, 75, 392, 677, 708, 962
AclWI GGATC 2 cut(s) 179, 339
AcsI RAATTY 2 cut(s) 584, 1002
AcuI CTGAAG 2 cut(s) 253, 516
AfaI GTAC 2 cut(s) 178, 204
AfiI CCNNNNNNNGG 3 cut(s) 391, 411, 721
AgeI ACCGGT 1 cut(s) 1060
AgsI TTSAA 9 cut(s) 162, 209, 242, 326, 466, 506, 554, 620, 977
AjiI CACGTC 1 cut(s) 110
AluBI AGCT 8 cut(s) 20, 85, 105, 264, 746, 779, 833, 866
AluI AGCT 8 cut(s) 20, 85, 105, 264, 746, 779, 833, 866
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 2 cut(s) 179, 339
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 5 cut(s) 256, 422, 648, 855, 934
ApeKI GCWGC 8 cut(s) 8, 102, 152, 389, 674, 779, 830, 866
ApoI RAATTY 2 cut(s) 584, 1002
AseI ATTAAT 1 cut(s) 837
AsiGI ACCGGT 1 cut(s) 1060
Asp700I GAANNNNTTC 1 cut(s) 767
AspLEI GCGC 2 cut(s) 146, 172
AspS9I GGNCC 4 cut(s) 257, 402, 649, 935
AsuHPI GGTGA 2 cut(s) 46, 1050
AsuNHI GCTAGC 1 cut(s) 862
AvaII GGWCC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 7 cut(s) 114, 139, 401, 686, 766, 842, 853
BccI CCATC 1 cut(s) 433
BceAI ACGGC 1 cut(s) 580
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciVI GTATCC 1 cut(s) 722
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 6 cut(s) 261, 581, 747, 842, 863, 983
BfuI GTATCC 1 cut(s) 722
BglII AGATCT 1 cut(s) 729
BisI GCNGC 8 cut(s) 9, 103, 153, 390, 675, 780, 831, 867
BlsI GCNGC 8 cut(s) 10, 104, 154, 391, 676, 781, 832, 868
BmcAI AGTACT 1 cut(s) 204
Bme18I GGWCC 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 110
BmgT120I GGNCC 4 cut(s) 257, 402, 649, 935
BmiI GGNNCC 1 cut(s) 988
BmrI ACTGGG 1 cut(s) 932
BmsI GCATC 3 cut(s) 385, 592, 1047
BmtI GCTAGC 1 cut(s) 866
BmuI ACTGGG 1 cut(s) 932
BpiI GAAGAC 1 cut(s) 504
BpuEI CTTGAG 4 cut(s) 356, 463, 526, 931
BsaBI GATNNNNATC 1 cut(s) 1037
BsaWI WCCGGW 1 cut(s) 1060
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 411, 721
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 1037
BseGI GGATG 3 cut(s) 400, 808, 970
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 3 cut(s) 391, 411, 721
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 216, 582, 643
BseNI ACTGG 1 cut(s) 938
BseRI GAGGAG 2 cut(s) 37, 353
BseXI GCAGC 7 cut(s) 114, 139, 401, 686, 766, 842, 853
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 5 cut(s) 258, 424, 650, 857, 936
BshTI ACCGGT 1 cut(s) 1060
BsiSI CCGG 1 cut(s) 1061
BslI CCNNNNNNNGG 3 cut(s) 391, 411, 721
BsmAI GTCTC 1 cut(s) 528
BsnI GGCC 5 cut(s) 258, 424, 650, 857, 936
Bsp143I GATC 5 cut(s) 184, 331, 346, 686, 729
Bsp68I TCGCGA 1 cut(s) 373
BspACI CCGC 7 cut(s) 6, 31, 75, 392, 677, 708, 962
BspANI GGCC 5 cut(s) 258, 424, 650, 857, 936
BspCNI CTCAG 3 cut(s) 217, 583, 642
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 988
BspOI GCTAGC 1 cut(s) 866
BspPI GGATC 2 cut(s) 179, 339
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 1060
BsrI ACTGG 1 cut(s) 938
BssAI RCCGGY 1 cut(s) 1060
BssMI GATC 5 cut(s) 184, 331, 346, 686, 729
Bst4CI ACNGT 1 cut(s) 614
Bst6I CTCTTC 1 cut(s) 321
BstAPI GCANNNNNTGC 2 cut(s) 395, 1019
BstC8I GCNNGC 5 cut(s) 447, 578, 648, 864, 1011
BstDEI CTNAG 5 cut(s) 81, 225, 591, 629, 772
BstEII GGTNACC 1 cut(s) 1056
BstF5I GGATG 3 cut(s) 400, 808, 970
BstFNI CGCG 1 cut(s) 373
BstHHI GCGC 2 cut(s) 146, 172
BstKTI GATC 5 cut(s) 187, 334, 349, 689, 732
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 5 cut(s) 184, 331, 346, 686, 729
BstNSI RCATGY 1 cut(s) 551
BstPI GGTNACC 1 cut(s) 1056
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 7 cut(s) 114, 139, 401, 686, 766, 842, 853
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 331, 729
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 331, 729
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 5 cut(s) 258, 424, 650, 857, 936
BtrI CACGTC 1 cut(s) 110
BtsCI GGATG 3 cut(s) 400, 808, 970
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 216, 654, 945
BtuMI TCGCGA 1 cut(s) 373
Cac8I GCNNGC 5 cut(s) 447, 578, 648, 864, 1011
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 2 cut(s) 146, 172
Cfr10I RCCGGY 1 cut(s) 1060
Cfr13I GGNCC 4 cut(s) 257, 402, 649, 935
Csp6I GTAC 2 cut(s) 177, 203
CspAI ACCGGT 1 cut(s) 1060
CviAII CATG 2 cut(s) 548, 919
CviQI GTAC 2 cut(s) 177, 203
DdeI CTNAG 5 cut(s) 81, 225, 591, 629, 772
DpnI GATC 5 cut(s) 186, 333, 348, 688, 731
DpnII GATC 5 cut(s) 184, 331, 346, 686, 729
DraI TTTAAA 2 cut(s) 663, 699
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 2 cut(s) 20, 977
Eco32I GATATC 1 cut(s) 902
Eco47I GGWCC 1 cut(s) 402
Eco57I CTGAAG 2 cut(s) 253, 516
Eco91I GGTNACC 1 cut(s) 1056
EcoO109I RGGNCCY 1 cut(s) 649
EcoO65I GGTNACC 1 cut(s) 1056
EcoRI GAATTC 1 cut(s) 584
EcoRV GATATC 1 cut(s) 902
FaeI CATG 2 cut(s) 551, 922
FalI AAGNNNNNCTT 4 cut(s) 318, 350, 755, 787
FatI CATG 2 cut(s) 547, 918
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 8 cut(s) 9, 103, 153, 390, 675, 780, 831, 867
FokI GGATG 3 cut(s) 407, 815, 977
Fsp4HI GCNGC 8 cut(s) 9, 103, 153, 390, 675, 780, 831, 867
FspBI CTAG 6 cut(s) 261, 581, 747, 842, 863, 983
GlaI GCGC 2 cut(s) 145, 171
GluI GCNGC 8 cut(s) 9, 103, 153, 390, 675, 780, 831, 867
HaeIII GGCC 5 cut(s) 258, 424, 650, 857, 936
HapII CCGG 1 cut(s) 1061
HhaI GCGC 2 cut(s) 146, 172
Hin1II CATG 2 cut(s) 551, 922
Hin6I GCGC 2 cut(s) 144, 170
HinP1I GCGC 2 cut(s) 144, 170
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 7 cut(s) 221, 246, 368, 375, 557, 627, 845
HpaII CCGG 1 cut(s) 1061
HphI GGTGA 2 cut(s) 46, 1050
Hpy166II GTNNAC 3 cut(s) 310, 405, 821
Hpy188I TCNGA 6 cut(s) 24, 96, 226, 592, 601, 686
Hpy188III TCNNGA 8 cut(s) 188, 335, 372, 561, 617, 842, 849, 897
Hpy8I GTNNAC 3 cut(s) 310, 405, 821
Hpy99I CGWCG 2 cut(s) 114, 311
HpyAV CCTTC 4 cut(s) 49, 530, 634, 757
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4IV ACGT 2 cut(s) 109, 306
HpyCH4V TGCA 8 cut(s) 102, 317, 449, 646, 869, 884, 998, 1028
HpyF3I CTNAG 5 cut(s) 81, 225, 591, 629, 772
HpySE526I ACGT 2 cut(s) 109, 306
Hsp92II CATG 2 cut(s) 551, 922
HspAI GCGC 2 cut(s) 144, 170
Kzo9I GATC 5 cut(s) 184, 331, 346, 686, 729
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 9 cut(s) 201, 546, 632, 707, 744, 862, 870, 944, 951
Lsp1109I GCAGC 7 cut(s) 114, 139, 401, 686, 766, 842, 853
LweI GCATC 3 cut(s) 385, 592, 1047
MaeI CTAG 6 cut(s) 261, 581, 747, 842, 863, 983
MaeII ACGT 2 cut(s) 109, 306
MaeIII GTNAC 2 cut(s) 927, 1056
MalI GATC 5 cut(s) 186, 333, 348, 688, 731
MboI GATC 5 cut(s) 184, 331, 346, 686, 729
MboII GAAGA 3 cut(s) 338, 509, 566
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 2 cut(s) 331, 729
MluCI AATT 6 cut(s) 485, 584, 737, 824, 1002, 1014
MlyI GAGTC 2 cut(s) 377, 621
MmeI TCCRAC 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 767
MseI TTAA 3 cut(s) 662, 698, 837
MslI CAYNNNNRTG 1 cut(s) 977
MspA1I CMGCKG 3 cut(s) 8, 392, 677
MspI CCGG 1 cut(s) 1061
MunI CAATTG 1 cut(s) 737
MvnI CGCG 1 cut(s) 373
NdeII GATC 5 cut(s) 184, 331, 346, 686, 729
NheI GCTAGC 1 cut(s) 862
NlaIII CATG 2 cut(s) 551, 922
NlaIV GGNNCC 1 cut(s) 988
NmuCI GTSAC 1 cut(s) 1056
NruI TCGCGA 1 cut(s) 373
NspI RCATGY 1 cut(s) 551
PdmI GAANNNNTTC 1 cut(s) 767
PfeI GAWTC 5 cut(s) 221, 246, 375, 557, 845
PflFI GACNNNGTC 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 1060
PkrI GCNGC 8 cut(s) 10, 104, 154, 391, 676, 781, 832, 868
PleI GAGTC 2 cut(s) 376, 621
PpsI GAGTC 2 cut(s) 376, 621
PshBI ATTAAT 1 cut(s) 837
PspEI GGTNACC 1 cut(s) 1056
PspN4I GGNNCC 1 cut(s) 988
PspPI GGNCC 4 cut(s) 257, 402, 649, 935
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 2 cut(s) 331, 729
PsyI GACNNNGTC 1 cut(s) 630
RruI TCGCGA 1 cut(s) 373
RsaI GTAC 2 cut(s) 178, 204
RsaNI GTAC 2 cut(s) 177, 203
RseI CAYNNNNRTG 1 cut(s) 977
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 3 cut(s) 662, 698, 837
SatI GCNGC 8 cut(s) 9, 103, 153, 390, 675, 780, 831, 867
Sau3AI GATC 5 cut(s) 184, 331, 346, 686, 729
Sau96I GGNCC 4 cut(s) 257, 402, 649, 935
ScaI AGTACT 1 cut(s) 204
SchI GAGTC 2 cut(s) 377, 621
SfaNI GCATC 3 cut(s) 385, 592, 1047
SinI GGWCC 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 977
SmlI CTYRAG 4 cut(s) 335, 478, 541, 910
SmoI CTYRAG 4 cut(s) 335, 478, 541, 910
Sse9I AATT 6 cut(s) 485, 584, 737, 824, 1002, 1014
SsiI CCGC 7 cut(s) 6, 31, 75, 392, 677, 708, 962
SspMI CTAG 6 cut(s) 261, 581, 747, 842, 863, 983
TaaI ACNGT 1 cut(s) 614
TaiI ACGT 2 cut(s) 112, 309
TaqI TCGA 2 cut(s) 309, 570
TaqII GACCGA 1 cut(s) 390
TasI AATT 6 cut(s) 485, 584, 737, 824, 1002, 1014
TatI WGTACW 1 cut(s) 202
TfiI GAWTC 5 cut(s) 221, 246, 375, 557, 845
Tru1I TTAA 3 cut(s) 662, 698, 837
Tru9I TTAA 3 cut(s) 662, 698, 837
TscAI CASTG 3 cut(s) 223, 661, 945
TseFI GTSAC 1 cut(s) 1056
TseI GCWGC 8 cut(s) 8, 102, 152, 389, 674, 779, 830, 866
Tsp45I GTSAC 1 cut(s) 1056
TspDTI ATGAA 1 cut(s) 512
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 3 cut(s) 223, 661, 945
Tth111I GACNNNGTC 1 cut(s) 630
VpaK11BI GGWCC 1 cut(s) 402
VspI ATTAAT 1 cut(s) 837
XapI RAATTY 2 cut(s) 584, 1002
XbaI TCTAGA 1 cut(s) 841
XceI RCATGY 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 767
XspI CTAG 6 cut(s) 261, 581, 747, 842, 863, 983
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.