Rroxscaffold_4G00276580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005892
Physical Location & Seq
Forward (+)
142076 .. 144129
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276580.1

Sequence Viewer

Length: 504 bp
ATGGCGATCTGCTCGGCTCCTCTAACCACTTCATTCTCAGTTGGCAGCGGATCTCAGCAGAAAGCGCGTGAGATTTGGTTGTCCGGCAAGTCGGAATCAGTAAAACTCACCGCACAGAGCCAGAGCCGGAGAAACTCGGTTCAATTACGAAACAACCGTAATTTCTGTGTTCGTGCTGAGTATAGTGATGGTAATAGAGGTGGAGGTGCTGATTTTCTTGCTGGTTTCCTGGTCGGAGGTGCTGTGTTTGGAACTTTAGCTTATGTTTTTGCTCCACAGATCAGAAGATCTCTCCTCAATGAAGATGAATATGGATTTCGTAAGGCCAGGAGACCAATATATTATGACGAAGGTTTAGAGAAGACCAGGCAGACCTTGAATGCAAAAATAAGCCAGCTCAATTCAGCCATTGACAATGTTTCTTCCCGCTTGAGAGGTGGGAACAATGCGCCCGCGGCGCCAGTCCCAGTCCAAAGTGATCCCGAAGTAGAATCTACCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.17

Weight (kDa)

9.63

Isoelectric Point (pI)

60.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 457
AccII CGCG 2 cut(s) 67, 455
AciI CCGC 5 cut(s) 48, 111, 427, 453, 455
AclWI GGATC 2 cut(s) 58, 473
AcyI GRCGYC 1 cut(s) 458
AgsI TTSAA 2 cut(s) 143, 379
AjnI CCWGG 3 cut(s) 228, 326, 365
AluBI AGCT 2 cut(s) 260, 397
AluI AGCT 2 cut(s) 260, 397
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 2 cut(s) 58, 473
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 1 cut(s) 45
AspLEI GCGC 3 cut(s) 67, 451, 460
AsuHPI GGTGA 1 cut(s) 100
BanI GGYRCC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 57
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 3 cut(s) 230, 328, 367
BcoDI GTCTC 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 461
BglII AGATCT 1 cut(s) 287
BisI GCNGC 2 cut(s) 46, 456
BlsI GCNGC 2 cut(s) 47, 457
Bme1390I CCNGG 3 cut(s) 230, 328, 367
BmiI GGNNCC 2 cut(s) 18, 459
BmrFI CCNGG 3 cut(s) 230, 328, 367
BmrI ACTGGG 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 451
BsaHI GRCGYC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 453
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bse1I ACTGG 2 cut(s) 461, 467
BseBI CCWGG 3 cut(s) 230, 328, 367
BseDI CCNNGG 1 cut(s) 453
BseMII CTCAG 3 cut(s) 51, 68, 168
BseNI ACTGG 2 cut(s) 461, 467
BseRI GAGGAG 2 cut(s) 9, 284
BseXI GCAGC 1 cut(s) 57
Bsh1236I CGCG 2 cut(s) 67, 455
BshFI GGCC 1 cut(s) 326
BshNI GGYRCC 1 cut(s) 457
BsiSI CCGG 2 cut(s) 84, 127
BslFI GGGAC 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 325
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 325
Bsp143I GATC 5 cut(s) 6, 50, 279, 287, 478
BspACI CCGC 5 cut(s) 48, 111, 427, 453, 455
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 3 cut(s) 50, 67, 169
BspFNI CGCG 2 cut(s) 67, 455
BspLI GGNNCC 2 cut(s) 18, 459
BspPI GGATC 2 cut(s) 58, 473
BspT107I GGYRCC 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 325
BsrI ACTGG 2 cut(s) 461, 467
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 5 cut(s) 6, 50, 279, 287, 478
BssNI GRCGYC 1 cut(s) 458
Bst2UI CCWGG 3 cut(s) 230, 328, 367
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 395, 453
BstDEI CTNAG 3 cut(s) 37, 54, 177
BstDSI CCRYGG 1 cut(s) 453
BstFNI CGCG 2 cut(s) 67, 455
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 3 cut(s) 67, 451, 460
BstKTI GATC 5 cut(s) 9, 53, 282, 290, 481
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 5 cut(s) 6, 50, 279, 287, 478
BstMWI GCNNNNNNNGC 3 cut(s) 64, 455, 457
BstNI CCWGG 3 cut(s) 230, 328, 367
BstSCI CCNGG 3 cut(s) 228, 326, 365
BstUI CGCG 2 cut(s) 67, 455
BstV1I GCAGC 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 50, 287
BstYI RGATCY 2 cut(s) 50, 287
BsuRI GGCC 1 cut(s) 326
BtgI CCRYGG 1 cut(s) 453
Cac8I GCNNGC 2 cut(s) 395, 453
CfoI GCGC 3 cut(s) 67, 451, 460
Cfr42I CCGCGG 1 cut(s) 456
CviAII CATG 1 cut(s) 499
CviJI RGCY 8 cut(s) 17, 120, 126, 260, 326, 393, 397, 407
CviKI_1 RGCY 8 cut(s) 17, 120, 126, 260, 326, 393, 397, 407
DdeI CTNAG 3 cut(s) 37, 54, 177
DinI GGCGCC 1 cut(s) 459
DpnI GATC 5 cut(s) 8, 52, 281, 289, 480
DpnII GATC 5 cut(s) 6, 50, 279, 287, 478
Eco31I GGTCTC 1 cut(s) 325
EcoRII CCWGG 3 cut(s) 228, 326, 365
EgeI GGCGCC 1 cut(s) 459
EheI GGCGCC 1 cut(s) 459
FaeI CATG 1 cut(s) 502
FaiI YATR 6 cut(s) 183, 264, 312, 340, 345, 500
FaqI GGGAC 1 cut(s) 449
FatI CATG 1 cut(s) 498
FauI CCCGC 2 cut(s) 434, 460
Fnu4HI GCNGC 2 cut(s) 46, 456
Fsp4HI GCNGC 2 cut(s) 46, 456
GlaI GCGC 3 cut(s) 66, 450, 459
GluI GCNGC 2 cut(s) 46, 456
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 1 cut(s) 326
HapII CCGG 2 cut(s) 84, 127
HhaI GCGC 3 cut(s) 67, 451, 460
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 1 cut(s) 502
Hin6I GCGC 3 cut(s) 65, 449, 458
HinP1I GCGC 3 cut(s) 65, 449, 458
HinfI GANTC 2 cut(s) 95, 491
HpaII CCGG 2 cut(s) 84, 127
HphI GGTGA 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 94, 236, 284
Hpy188III TCNNGA 1 cut(s) 482
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 455, 457
HpyF3I CTNAG 3 cut(s) 37, 54, 177
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 1 cut(s) 502
HspAI GCGC 3 cut(s) 65, 449, 458
KasI GGCGCC 1 cut(s) 457
KspI CCGCGG 1 cut(s) 456
Kzo9I GATC 5 cut(s) 6, 50, 279, 287, 478
LmnI GCTCC 2 cut(s) 22, 277
Lsp1109I GCAGC 1 cut(s) 57
MalI GATC 5 cut(s) 8, 52, 281, 289, 480
MboI GATC 5 cut(s) 6, 50, 279, 287, 478
MboII GAAGA 4 cut(s) 297, 314, 373, 414
MflI RGATCY 2 cut(s) 50, 287
MluCI AATT 3 cut(s) 143, 160, 400
Mly113I GGCGCC 1 cut(s) 458
MmeI TCCRAC 2 cut(s) 72, 214
MnlI CCTC 6 cut(s) 30, 191, 197, 230, 305, 428
MspA1I CMGCKG 2 cut(s) 48, 455
MspI CCGG 2 cut(s) 84, 127
MspR9I CCNGG 3 cut(s) 230, 328, 367
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 3 cut(s) 230, 328, 367
MvnI CGCG 2 cut(s) 67, 455
MwoI GCNNNNNNNGC 3 cut(s) 64, 455, 457
NarI GGCGCC 1 cut(s) 458
NdeII GATC 5 cut(s) 6, 50, 279, 287, 478
NlaIII CATG 1 cut(s) 502
NlaIV GGNNCC 2 cut(s) 18, 459
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 2 cut(s) 95, 491
PkrI GCNGC 2 cut(s) 47, 457
PluTI GGCGCC 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 228, 326, 365
PspGI CCWGG 3 cut(s) 228, 326, 365
PspN4I GGNNCC 2 cut(s) 18, 459
PsuI RGATCY 2 cut(s) 50, 287
SacII CCGCGG 1 cut(s) 456
SatI GCNGC 2 cut(s) 46, 456
Sau3AI GATC 5 cut(s) 6, 50, 279, 287, 478
ScrFI CCNGG 3 cut(s) 230, 328, 367
SetI ASST 8 cut(s) 202, 208, 241, 262, 355, 377, 399, 439
SfoI GGCGCC 1 cut(s) 459
Sfr303I CCGCGG 1 cut(s) 456
SgrBI CCGCGG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 3 cut(s) 143, 160, 400
SsiI CCGC 5 cut(s) 48, 111, 427, 453, 455
SspDI GGCGCC 1 cut(s) 457
StyD4I CCNGG 3 cut(s) 228, 326, 365
TaaI ACNGT 1 cut(s) 158
TasI AATT 3 cut(s) 143, 160, 400
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 2 cut(s) 95, 491
TseI GCWGC 1 cut(s) 45
TspDTI ATGAA 3 cut(s) 21, 315, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.