Rorug01G0449200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005892
Physical Location & Seq
Reverse (-)
54504542 .. 54505914
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0449200.1

Sequence Viewer

Length: 1257 bp
ATGGATCAGATCAAAGTGAGTGAATCGGAAAGGGAGAATGTGAATCGGAATCGGAATCGCAAACGGAGTCCGAATCCGAGTTCGAATCGCAACCCCAAATTGTATGTATGTTGCCAGGAAGAAAACATCTTCAGGGGCTGCTACGTCATCCGCGCCATCGATTTGGAGTGTTTGATGGCTTGCTCCTGTGACTTTGATGCAGACAAAGGTGTGGAAAACAAGTGCGCTAGCTGCGAATCCTGCCCCAATCATGATTTGCGGCCAGTCGGTTTACTCTGCGGCGATTACTGCGATATCCCCGACTGCATGAGCTGCTGTCCATTTGGCTCCCAGGTTGTCTTCGTAGGCGGCGAGTTATCACCGTGGTCGTATGATTGCAGACCCAGCCGAAAGGTTCTCGTCTATGACATTGAGGATGAGGATGGGGATGAGCTCCGGCTTCAGGGCTTAGAAAACGGCAACAACATCAAGGAGGAGTTGGACAAAAGCTGCAACGGTCTTGCAAATGACATATATTATCCGCGGATGATGGAGATAGGAGGAGATCTCTATGCTCTCGGAGTTGTCAAATGTGACGTCAGTGTCTCTCAGGTATACAGGAGCCAATCGCAGCGGTGGGAAGCTCTGCCTCCACCCATACCGAGTTTCAGTCGTGATTTCACTAGTGAAACTCTATATTCTCTACTTTACACGGACAACAAGATCTTTCTGTCCACCTATTTCCCACATATGCCGGTCAAATGTTTCGACACCGACCGTAAATGCTGGATAGCTGATTCCGGCTTCCACAGTGAGGTTTTCCCCTTTGTGGGTGAGGCTTTAGTCGTGGACTTGGATAAACCTTGCAAGGAGAAGTTGGTGTTTACCTATGATCGTGATATTATAAACGTGTGGCGATTGGGATTGACTGATGATTTGAAAGGTTCTTATCTTACCAGCTACGGGTCTGTGAATATGAAACCCAGGTTGCTGGGCTTATTGGAAAATTTGCGTGAAATATCTAAGCAGTTTCGTCTCATCGATCTAGGAGATGGAAAAATCTGCTTTGTTAGGGTCAGGAGCTACCCGGTTGAGGAACGTACTGTAAATTCACTAGGGCTTGAGAAGGTGCGTGTTGTTATCATAGTATTCAAATATGAGTTTGATCCGAGAGCACAAGCCTTGCCCTACAAGATCCTGTCTACCCGCGCCTTTACCATCATGCCCCATTGCAAGGAATCCTGCTTTGTCCGCCTGCTTAAATGCTTTGTGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

47.87

Weight (kDa)

5.54

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 48 - 356 2.7e-08 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AasI GACNNNNNNGTC 1 cut(s) 581
AatII GACGTC 1 cut(s) 579
AccI GTMKAC 2 cut(s) 594, 1181
AccII CGCG 3 cut(s) 153, 523, 1188
AciI CCGC 9 cut(s) 151, 259, 279, 348, 521, 523, 613, 1186, 1231
AclWI GGATC 3 cut(s) 12, 1139, 1168
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 2 cut(s) 985, 1087
AcuI CTGAAG 2 cut(s) 115, 425
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 1 cut(s) 1081
AfiI CCNNNNNNNGG 7 cut(s) 442, 793, 808, 809, 942, 1072, 1213
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 2 cut(s) 919, 1132
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 3 cut(s) 114, 330, 962
AluBI AGCT 8 cut(s) 231, 312, 433, 489, 623, 773, 939, 1062
AluI AGCT 8 cut(s) 231, 312, 433, 489, 623, 773, 939, 1062
Alw21I GWGCWC 2 cut(s) 435, 1156
Alw26I GTCTC 2 cut(s) 589, 1019
AlwI GGATC 3 cut(s) 12, 1139, 1168
AlwNI CAGNNNCTG 1 cut(s) 138
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 5 cut(s) 138, 231, 312, 489, 610
ApoI RAATTY 2 cut(s) 985, 1087
ArsI GACNNNNNNTTYG 4 cut(s) 561, 593, 898, 930
AspLEI GCGC 4 cut(s) 155, 227, 1190, 1254
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 2 cut(s) 351, 824
AsuII TTCGAA 1 cut(s) 83
AsuNHI GCTAGC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 435, 1156
BbvI GCAGC 5 cut(s) 125, 218, 299, 476, 622
BccI CCATC 6 cut(s) 164, 169, 416, 523, 1025, 1205
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 3 cut(s) 116, 332, 964
BcnI CCSGG 1 cut(s) 1067
BcoDI GTCTC 2 cut(s) 589, 1019
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 5 cut(s) 228, 663, 1025, 1094, 1255
BglII AGATCT 2 cut(s) 544, 702
BisI GCNGC 8 cut(s) 139, 232, 260, 280, 313, 349, 490, 611
BlsI GCNGC 8 cut(s) 140, 233, 261, 281, 314, 350, 491, 612
Bme1390I CCNGG 4 cut(s) 116, 332, 964, 1067
BmiI GGNNCC 2 cut(s) 328, 602
BmrFI CCNGG 4 cut(s) 116, 332, 964, 1067
BmsI GCATC 1 cut(s) 187
BmtI GCTAGC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 331
BplI GAGNNNNNCTC 2 cut(s) 531, 563
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 1121
BpuMI CCSGG 1 cut(s) 1067
Bsa29I ATCGAT 2 cut(s) 159, 1020
BsaHI GRCGYC 1 cut(s) 576
BsaJI CCNNGG 4 cut(s) 330, 362, 521, 962
Bsc4I CCNNNNNNNGG 7 cut(s) 442, 793, 808, 809, 942, 1072, 1213
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 1207
BseBI CCWGG 3 cut(s) 116, 332, 964
BseCI ATCGAT 2 cut(s) 159, 1020
BseDI CCNNGG 4 cut(s) 330, 362, 521, 962
BseGI GGATG 5 cut(s) 147, 421, 427, 433, 531
BseLI CCNNNNNNNGG 7 cut(s) 442, 793, 808, 809, 942, 1072, 1213
BseMI GCAATG 1 cut(s) 1207
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 2 cut(s) 488, 555
BseXI GCAGC 5 cut(s) 125, 218, 299, 476, 622
BseYI CCCAGC 2 cut(s) 383, 970
Bsh1236I CGCG 3 cut(s) 153, 523, 1188
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 1 cut(s) 262
BshVI ATCGAT 2 cut(s) 159, 1020
BsiEI CGRYCG 1 cut(s) 757
BsiHKAI GWGCWC 2 cut(s) 435, 1156
BsiSI CCGG 4 cut(s) 436, 734, 780, 1067
BslI CCNNNNNNNGG 7 cut(s) 442, 793, 808, 809, 942, 1072, 1213
BsmAI GTCTC 2 cut(s) 589, 1019
BsmBI CGTCTC 1 cut(s) 1019
BsnI GGCC 1 cut(s) 262
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 435, 1156
Bsp143I GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
BspACI CCGC 9 cut(s) 151, 259, 279, 348, 521, 523, 613, 1186, 1231
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 601
BspDI ATCGAT 2 cut(s) 159, 1020
BspFNI CGCG 3 cut(s) 153, 523, 1188
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 328, 602
BspOI GCTAGC 1 cut(s) 231
BspPI GGATC 3 cut(s) 12, 1139, 1168
BspT104I TTCGAA 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 1207
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 4 cut(s) 330, 362, 521, 962
BssMI GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
BssNAI GTATAC 1 cut(s) 595
BssNI GRCGYC 1 cut(s) 576
Bst1107I GTATAC 1 cut(s) 595
Bst2UI CCWGG 3 cut(s) 116, 332, 964
Bst4CI ACNGT 5 cut(s) 363, 497, 758, 791, 1084
BstACI GRCGYC 1 cut(s) 576
BstAPI GCANNNNNTGC 1 cut(s) 312
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 3 cut(s) 181, 229, 1235
BstDEI CTNAG 3 cut(s) 448, 588, 1002
BstDSI CCRYGG 2 cut(s) 362, 521
BstF5I GGATG 5 cut(s) 147, 421, 427, 433, 531
BstFNI CGCG 3 cut(s) 153, 523, 1188
BstHHI GCGC 4 cut(s) 155, 227, 1190, 1254
BstKTI GATC 8 cut(s) 7, 12, 547, 705, 874, 1024, 1147, 1176
BstMAI GTCTC 2 cut(s) 589, 1019
BstMBI GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
BstMCI CGRYCG 1 cut(s) 757
BstMWI GCNNNNNNNGC 7 cut(s) 231, 240, 288, 312, 384, 1230, 1251
BstNI CCWGG 3 cut(s) 116, 332, 964
BstSCI CCNGG 4 cut(s) 114, 330, 962, 1065
BstUI CGCG 3 cut(s) 153, 523, 1188
BstV1I GCAGC 5 cut(s) 125, 218, 299, 476, 622
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 3 cut(s) 544, 702, 1173
BstXI CCANNNNNNTGG 2 cut(s) 163, 970
BstYI RGATCY 3 cut(s) 544, 702, 1173
BstZ17I GTATAC 1 cut(s) 595
Bsu15I ATCGAT 2 cut(s) 159, 1020
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 2 cut(s) 159, 1020
BtgI CCRYGG 2 cut(s) 362, 521
BtsCI GGATG 5 cut(s) 147, 421, 427, 433, 531
BtsIMutI CAGTG 2 cut(s) 586, 796
Cac8I GCNNGC 3 cut(s) 181, 229, 1235
CaiI CAGNNNCTG 1 cut(s) 138
CciI TCATGA 1 cut(s) 250
CfoI GCGC 4 cut(s) 155, 227, 1190, 1254
Cfr10I RCCGGY 1 cut(s) 733
Cfr42I CCGCGG 1 cut(s) 524
ClaI ATCGAT 2 cut(s) 159, 1020
Csp6I GTAC 1 cut(s) 1080
CviAII CATG 3 cut(s) 251, 307, 1201
CviQI GTAC 1 cut(s) 1080
DdeI CTNAG 3 cut(s) 448, 588, 1002
DpnI GATC 8 cut(s) 6, 11, 546, 704, 873, 1023, 1146, 1175
DpnII GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 260
EciI GGCGGA 1 cut(s) 1220
Ecl136II GAGCTC 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 115, 425
EcoICRI GAGCTC 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 114, 330, 962
EcoRV GATATC 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 1019
FaeI CATG 3 cut(s) 254, 310, 1204
FatI CATG 3 cut(s) 250, 306, 1200
FauI CCCGC 1 cut(s) 1193
FauNDI CATATG 1 cut(s) 729
FblI GTMKAC 2 cut(s) 594, 1181
Fnu4HI GCNGC 8 cut(s) 139, 232, 260, 280, 313, 349, 490, 611
FokI GGATG 5 cut(s) 134, 428, 434, 440, 538
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 8 cut(s) 139, 232, 260, 280, 313, 349, 490, 611
FspBI CTAG 5 cut(s) 228, 663, 1025, 1094, 1255
GlaI GCGC 4 cut(s) 154, 226, 1189, 1253
GluI GCNGC 8 cut(s) 139, 232, 260, 280, 313, 349, 490, 611
GsaI CCCAGC 2 cut(s) 387, 974
HaeIII GGCC 1 cut(s) 262
HapII CCGG 4 cut(s) 436, 734, 780, 1067
HhaI GCGC 4 cut(s) 155, 227, 1190, 1254
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 3 cut(s) 254, 310, 1204
Hin6I GCGC 4 cut(s) 153, 225, 1188, 1252
HinP1I GCGC 4 cut(s) 153, 225, 1188, 1252
HpaII CCGG 4 cut(s) 436, 734, 780, 1067
HphI GGTGA 2 cut(s) 351, 824
Hpy166II GTNNAC 6 cut(s) 272, 595, 714, 829, 864, 1182
Hpy188I TCNGA 8 cut(s) 9, 28, 48, 54, 72, 78, 560, 1149
Hpy188III TCNNGA 4 cut(s) 251, 653, 875, 1057
Hpy8I GTNNAC 6 cut(s) 272, 595, 714, 829, 864, 1182
HpyAV CCTTC 1 cut(s) 1099
HpyCH4III ACNGT 5 cut(s) 363, 497, 758, 791, 1084
HpyCH4IV ACGT 4 cut(s) 144, 576, 888, 1078
HpyCH4V TGCA 7 cut(s) 200, 306, 378, 492, 503, 846, 1212
HpyF10VI GCNNNNNNNGC 7 cut(s) 231, 240, 288, 312, 384, 1230, 1251
HpyF3I CTNAG 3 cut(s) 448, 588, 1002
HpySE526I ACGT 4 cut(s) 144, 576, 888, 1078
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 3 cut(s) 254, 310, 1204
HspAI GCGC 4 cut(s) 153, 225, 1188, 1252
KspI CCGCGG 1 cut(s) 524
Kzo9I GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
LmnI GCTCC 5 cut(s) 188, 332, 438, 600, 1059
Lsp1109I GCAGC 5 cut(s) 125, 218, 299, 476, 622
LweI GCATC 1 cut(s) 187
MaeI CTAG 5 cut(s) 228, 663, 1025, 1094, 1255
MaeII ACGT 4 cut(s) 144, 576, 888, 1078
MaeIII GTNAC 2 cut(s) 188, 572
MalI GATC 8 cut(s) 6, 11, 546, 704, 873, 1023, 1146, 1175
MboI GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
MboII GAAGA 3 cut(s) 121, 131, 331
MflI RGATCY 3 cut(s) 544, 702, 1173
MhlI GDGCHC 2 cut(s) 435, 1156
MluCI AATT 3 cut(s) 98, 985, 1087
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 8 cut(s) 406, 412, 466, 533, 639, 787, 808, 1066
MseI TTAA 1 cut(s) 1239
MspA1I CMGCKG 2 cut(s) 523, 613
MspI CCGG 4 cut(s) 436, 734, 780, 1067
MspR9I CCNGG 4 cut(s) 116, 332, 964, 1067
MvaI CCWGG 3 cut(s) 116, 332, 964
MvnI CGCG 3 cut(s) 153, 523, 1188
MwoI GCNNNNNNNGC 7 cut(s) 231, 240, 288, 312, 384, 1230, 1251
NciI CCSGG 1 cut(s) 1067
NdeI CATATG 1 cut(s) 729
NdeII GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
NheI GCTAGC 1 cut(s) 227
NlaIII CATG 3 cut(s) 254, 310, 1204
NlaIV GGNNCC 2 cut(s) 328, 602
NmuCI GTSAC 2 cut(s) 188, 572
NspV TTCGAA 1 cut(s) 83
PagI TCATGA 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 9 cut(s) 23, 43, 49, 55, 73, 85, 236, 776, 1217
PkrI GCNGC 8 cut(s) 140, 233, 261, 281, 314, 350, 491, 612
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PsiI TTATAA 1 cut(s) 884
Psp124BI GAGCTC 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 114, 330, 962
PspFI CCCAGC 2 cut(s) 383, 970
PspGI CCWGG 3 cut(s) 114, 330, 962
PspN4I GGNNCC 2 cut(s) 328, 602
PstNI CAGNNNCTG 1 cut(s) 138
PsuI RGATCY 3 cut(s) 544, 702, 1173
RsaI GTAC 1 cut(s) 1081
RsaNI GTAC 1 cut(s) 1080
SacI GAGCTC 1 cut(s) 435
SacII CCGCGG 1 cut(s) 524
SaqAI TTAA 1 cut(s) 1239
SatI GCNGC 8 cut(s) 139, 232, 260, 280, 313, 349, 490, 611
Sau3AI GATC 8 cut(s) 4, 9, 544, 702, 871, 1021, 1144, 1173
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 4 cut(s) 116, 332, 964, 1067
SduI GDGCHC 2 cut(s) 435, 1156
SfaNI GCATC 1 cut(s) 187
Sfr303I CCGCGG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 3 cut(s) 98, 985, 1087
SsiI CCGC 9 cut(s) 151, 259, 279, 348, 521, 523, 613, 1186, 1231
SspMI CTAG 5 cut(s) 228, 663, 1025, 1094, 1255
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 114, 330, 962, 1065
TaaI ACNGT 5 cut(s) 363, 497, 758, 791, 1084
TaiI ACGT 4 cut(s) 147, 579, 891, 1081
TaqI TCGA 4 cut(s) 83, 159, 747, 1020
TasI AATT 3 cut(s) 98, 985, 1087
TauI GCSGC 3 cut(s) 262, 282, 351
TfiI GAWTC 9 cut(s) 23, 43, 49, 55, 73, 85, 236, 776, 1217
Tru1I TTAA 1 cut(s) 1239
Tru9I TTAA 1 cut(s) 1239
TscAI CASTG 2 cut(s) 586, 796
TseFI GTSAC 2 cut(s) 188, 572
TseI GCWGC 5 cut(s) 138, 231, 312, 489, 610
Tsp45I GTSAC 2 cut(s) 188, 572
TspDTI ATGAA 1 cut(s) 971
TspGWI ACGGA 2 cut(s) 79, 707
TspRI CASTG 2 cut(s) 586, 796
XapI RAATTY 2 cut(s) 985, 1087
XmiI GTMKAC 2 cut(s) 594, 1181
XspI CTAG 5 cut(s) 228, 663, 1025, 1094, 1255
ZraI GACGTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.