Rroxscaffold_4G00277370

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012655
Physical Location & Seq
Forward (+)
823673 .. 834644
10972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277370.1

Sequence Viewer

Length: 3564 bp
ATGCAGAATATGGAAACGAAGAAACAAGCAATCGATGAGGATGATGAGACGATGGGAAGCTCATCCGAAACGTTGGGATTCAGTAAGCTGCTAAGCTATGCGGACGGATGGGATTGGATTATGATGGTATTGGGGACGTTGGGTTCTGTTGTCCATGGTATGGCTTTTCCAGTTGGATATCTACTGCTCGGCAGAGCACTCGATGCTTTTGGGAACAATATCAACGACACACCTGCCATGGTTAAAGCACTGAACAAGGTTATTCCATATGTATGGTACATGGCCTTCGCCACATTTCCGGCTGGAATACTTGAAGTTGGATGCTGGATGTATTCAAGTGAAAGACAAGTGGCACGTCTAAGACTAGCATATCTAAGAGCAGTGCTCAGCCAGGAGGTTGGGGCTTTCGATACAGACTTAACCAGTGGAAAAGTGATCACCGGAATTAGTAACCACACTAGTATCATACACAGTGCAATCGGAGAGACGTTGGGGCATTTTCTGTCAAGTTTCGCAACTTTCTTTTCGGGGATTTTGATTGCTGCAATATGCTGTTGGGAAGTTGCACTGCTTACCTTGTTGGTTGTTCCTTTGATTCTTGTAATCGGAGCCACTTATACTAAGAAGATGAACGCTGTCTCGGCCGCTAGGATGCTTTACCTGGCGGAAGCTACCTCTATGGTAGAACAGACCATATGTCAAATCAAAACAGTATATGCATTCGTTGGAGAAAACTCGGCAATCAAATCTTTCTCAAACTGCATGGCCAAACAGTATTTGCTAAGCAAAGGGGAGGCTCTAGTAAAGGGATTTGGAACAGGAATGCTTCAATCTGTGACCTTCATTTCCTGGGCTATCATCATTTGGGTTGGTGCTGTTGTAGTCACTGCCAAGAGGGCAAGCGGTGGAGATATTATAGCCGCTGTCATGAGCATTCTCTTTGGAGCCATATCACTCACTTATGCTGCGCCGGACATGCAAGTCTTCAATCAAGCAAAGGCTGCAGGAACTGAAGTTTTCAAGGTGATCAATAGAAAGCCAGCTATAAGTTATGATTCACCAGGGAAGGAACTTGACAAGATTTACGGCAACATTGACATACATGATGTTTACTTTTCCTACCCTTCCCGGCCTGACAGAGCCATCCTTCAAGGCTTTTCATTGTCAATCCCAGCAGGAAAAACTGTAGCCTTTGTGGGCAGCAGTGGCTGTGGAAAGAGTACTATCATCTCACTAGTCACAAGATTCTATGATCCGCCAAAAGGTTTGGAGATTCTGATTGACAACCACAACATCAAGGATCTTGACTTGAAATTCCTGAGGAAAAACATAGGAGCTGTTTCGCAGGAACCATCACTGTTTGCAGGCACCATTGCGGACAACATGAAAGTCGGTAAGATGGATGCAGAAGATGTAGAGATCCAGAAAGCATCAGTGATGGCAAATGCACACACTTTCATATCTCAACTTCCAGATCAGTACTCCACAGAGGCTGATCTGCACAGGAATGAGCGTGCTGGACAGCTCCCACTTCGGATGGTGTACAGGTCTCAAAACTACTTCAACATGGATGAAATTTTGAGGCTGATCTGCACAGGAACCAGCGCAGGGGCAGCGCACGGACAAAGGGGAGTCCAGTTATCTGGAGGCCAGAAGCAGAGAATCGCCATAGCAAGAGCCATTCTCAAGAATCCTCCTATTCTTCTGCTTGATGAGGCAACAAGTGCTCTTGACTCAGAATCTGAGAAGGTGGTTCAAGAAGCACTTGACAAGGCTATGCAAGGAAGGACAGTCATCTTGATAGCACACAGACTATCAACTATTGTTAATGCAGATATTATTGCGGTTGTGGAAAATGGACAAGTTTCAGAAACAGGAACTCACCGGAGCTTACTTGAAAGCAGCAAATTCTACAACAACTTGTTTAACATGCAGAACCTCAACCCAGTTCATGAGTCGAGAGAAGATGGTCCCTCTGAAGAACATGAAAGCATCCACCAAAAGAACTCACCTGAACAGATAACAAGAGCTAAGGAGCCCCACAGCGACCGGTATCCTAATCAGCCTCCCAAGCAAGACGAAGAGGAAATAAGAAAACAAGCCATATTCTTCAGAATCTGGTTTGGCTTGAAAACGAGAGAACTCGTAAAGATTGCTATTGGTTCTTTTGCAGCAGCTTTCTCTGGCATATCAAAGCCTGTCTTTGGGTATTACATTATAACAATAGGAGTAGCATACTACCACCCAGGTGTGCTACGCAATGAAATTGGATGGTATGAGAACCCTGAGAATGGCATTGGACAGCTCACCTCAAGGATCATCAATGACACTTCAATGGTTAAACTGGTAATTGCTGACCGGATGTCTGTCATTGTTCAGTGCATCTCGTCCATACTGATTGCAACCATTGTGAGCATGTATGTCAACTGGAGAATGGGGTTGGTAGCTTGGGCTGTGATGCCTTGCCACTTCATTGGTGGTCTCATTCAAGCCAAGTCTGCCAAGGGTTTTGCAGGAGACTCAGCTGCTGCACATAATGAACTTGTTTCTCTTGCTTCTGAGTCTGCACAAAACATAAGAACCGTTGCGTCGTTTTGCCATGAAGATCATATACTCAAAAAAGCCGAAATATCTCTGGAAACACCCAAGAGAATGAGCAGGAAAGAAAGTATCAAGTATGGCATAATTCAGGGAGTTTCTCTTTGCTTATGGAATATTGCACATGCTGTGGCATTGTGGTACACAACAGTTTTGGTTCATAAAAATCAATCGAGCTTCAAAGATGGCATAAGATCATACCAGATTTTCTCTCTCACAGTACCTTCAATCACAGAATTGTGGACATTGATTCCCACTGTCATTTCTGCCATCAATGTGCTCACTCCTGCATTCGAGACCCTTGACCGCAAAACTAAAATAGATCCATCTACACCAGAAACTTCAAATCGGGACAGAATCAAAGGGAACATTGAATTTCAAAACGTTGAGTTTAATTACCCTTTGAGACCAGAAGTAACTATCCTGAACAACTTCAGTGTACAAATTGAAGCTGGTAAAAAGGTGGCTCTTGTCGGTCCAAGTGGAGCTGGAAAATCTTCAGTTCTGGCCCTTCTGCTAAGATTTTATGACCCCTTGAAAGGTAAGATACTCATTGATGGAAAGGAGATAAGGGAATACAATTTGAGATGGCTGAGAACACAAATAGGGTTGGTGCAACAAGAGCCTCTTCTTTTTAGCTCCTCAATCAGACACAATATCTGCTACGGGAACGAAAGTGCTTCTGAAACTGAGATTGTGGAGCAACAAGTGCCCCTTGATGCTGAATCTGAGAAATCTGTGGTGAGTGCATTGGAAGCGATAAATTTGAAAAAGAATGGGGGCCTGCTGTCAAAAACCACACAGATTACAGTTGCACATAGGCTTTCCACAATAATAAAGTCGGATATCATTGTTGTCATGGACAACGGTGAGATTGTGGAGATGGGATCCCACTCAACCCTAATAACAGCATCTGAGGGAGTTTATTCAAGATTATATCAGATACAGAGCATGGGAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1187

Amino Acids

130.49

Weight (kDa)

6.61

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 43 - 316 4.4e-45 ABC transporter transmembrane region
SMC_N PF02463 239 - 617 1.9e-06 RecF/RecN/SMC N terminal domain
ABC_tran PF00005 383 - 575 4.5e-29 ABC transporter
ABC_membrane PF00664 751 - 934 8.5e-34 ABC transporter transmembrane region
ABC_tran PF00005 1008 - 1112 1.1e-18 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012655)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g32300 FvH4_7g32320 FvH4_7g33380
malus_domestica MD01G1232000.v1.1 MD01G1232100.v1.1
prunus_persica Prupe.2G322600_v2.0.a1
pyrus_communis pycom01g24050
rosa_chinensis RchiOBHm_Chr1g0383351
rosa_laevigata RLG00000026110
rosa_multiflora Rmu_sc0007173.1_g000013
rosa_roxburghii Rroxscaffold_4G00277370
rosa_rugosa Rorug01G0440700
rosa_samantha Rh1AG466600 Rh1BG423900 Rh1BG424000 Rh1CG437100 Rh1DG456600 Rh1DG456700
rosa_wichuraiana Rw1G040590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2219
AarI CACCTGC 1 cut(s) 241
AasI GACNNNNNNGTC 1 cut(s) 980
Acc36I ACCTGC 1 cut(s) 241
AccB1I GGYRCC 1 cut(s) 1367
AccB7I CCANNNNNTGG 1 cut(s) 160
AciI CCGC 9 cut(s) 101, 645, 665, 903, 921, 1256, 1376, 1844, 2905
AclI AACGTT 2 cut(s) 71, 2982
AclWI GGATC 7 cut(s) 1247, 1308, 1414, 2324, 2915, 3480, 3493
AcoI YGGCCR 2 cut(s) 642, 765
AcsI RAATTY 5 cut(s) 1313, 1575, 1907, 2972, 3361
AcuI CTGAAG 5 cut(s) 1032, 1998, 2095, 3016, 3081
AfaI GTAC 7 cut(s) 278, 1222, 1481, 1544, 2741, 2820, 3039
AfiI CCNNNNNNNGG 6 cut(s) 160, 1262, 1608, 2204, 2291, 3137
AgeI ACCGGT 1 cut(s) 2049
AhdI GACNNNNNGTC 2 cut(s) 696, 2362
AhlI ACTAGT 2 cut(s) 458, 1234
AjiI CACGTC 1 cut(s) 356
AjnI CCWGG 5 cut(s) 390, 660, 848, 1060, 2245
AleI CACNNNNGTG 1 cut(s) 2247
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
Alw21I GWGCWC 4 cut(s) 199, 387, 1729, 2880
Alw26I GTCTC 8 cut(s) 41, 479, 643, 1554, 2486, 2511, 2888, 2998
AlwI GGATC 7 cut(s) 1247, 1308, 1414, 2324, 2915, 3480, 3493
AlwNI CAGNNNCTG 7 cut(s) 1010, 1209, 1493, 1742, 2118, 2528, 3512
AoxI GGCC 7 cut(s) 282, 642, 765, 1130, 1648, 3105, 3379
ApoI RAATTY 5 cut(s) 1313, 1575, 1907, 2972, 3361
AsiGI ACCGGT 1 cut(s) 2049
Asp700I GAANNNNTTC 2 cut(s) 3029, 3094
AspLEI GCGC 3 cut(s) 970, 1607, 1618
AspS9I GGNCC 4 cut(s) 1970, 3074, 3106, 3379
AsuC2I CCSGG 1 cut(s) 1129
AsuHPI GGTGA 8 cut(s) 430, 1036, 1050, 1874, 2001, 2299, 3352, 3479
AvaII GGWCC 2 cut(s) 1970, 3074
AxyI CCTNAGG 1 cut(s) 1319
BaeGI GKGCMC 1 cut(s) 3312
BaeI ACNNNNGTAYC 2 cut(s) 445, 478
BalI TGGCCA 1 cut(s) 767
BamHI GGATCC 1 cut(s) 3485
BanI GGYRCC 1 cut(s) 1367
BanII GRGCYC 1 cut(s) 2040
BarI GAAGNNNNNNTAC 4 cut(s) 269, 301, 2595, 2627
BbsI GAAGAC 1 cut(s) 976
Bbv12I GWGCWC 4 cut(s) 199, 387, 1729, 2880
BceAI ACGGC 1 cut(s) 1102
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 215, 249
BciT130I CCWGG 5 cut(s) 392, 662, 850, 1062, 2247
BciVI GTATCC 1 cut(s) 2064
BclI TGATCA 2 cut(s) 435, 1026
BcnI CCSGG 1 cut(s) 1129
BcoDI GTCTC 8 cut(s) 41, 479, 643, 1554, 2486, 2511, 2888, 2998
BcuI ACTAGT 2 cut(s) 458, 1234
BfaI CTAG 5 cut(s) 365, 459, 648, 800, 1235
BfmI CTRYAG 2 cut(s) 1002, 1185
BfuAI ACCTGC 1 cut(s) 241
BfuI GTATCC 1 cut(s) 2064
BglI GCCNNNNNGGC 1 cut(s) 896
BlpI GCTNAGC 3 cut(s) 92, 386, 782
BmcAI AGTACT 2 cut(s) 1222, 1481
Bme1390I CCNGG 6 cut(s) 392, 662, 850, 1062, 1129, 2247
Bme18I GGWCC 2 cut(s) 1970, 3074
BmeRI GACNNNNNGTC 2 cut(s) 696, 2362
BmgBI CACGTC 1 cut(s) 356
BmgT120I GGNCC 4 cut(s) 1970, 3074, 3106, 3379
BmiI GGNNCC 9 cut(s) 610, 946, 1350, 1369, 1600, 1972, 2037, 3380, 3487
BmrFI CCNGG 6 cut(s) 392, 662, 850, 1062, 1129, 2247
BmrI ACTGGG 1 cut(s) 1940
BmuI ACTGGG 1 cut(s) 1940
BpiI GAAGAC 1 cut(s) 976
BplI GAGNNNNNCTC 4 cut(s) 369, 401, 1668, 1700
BpmI CTGGAG 2 cut(s) 1665, 2449
Bpu10I CCTNAGC 1 cut(s) 2031
Bpu1102I GCTNAGC 3 cut(s) 92, 386, 782
BpuEI CTTGAG 2 cut(s) 1670, 2296
BpuMI CCSGG 1 cut(s) 1129
Bsa29I ATCGAT 1 cut(s) 33
BsaI GGTCTC 4 cut(s) 1554, 2486, 2888, 2998
BsaJI CCNNGG 6 cut(s) 154, 237, 849, 1061, 2245, 2502
BsaWI WCCGGW 4 cut(s) 440, 1884, 2049, 2358
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 1262, 1608, 2204, 2291, 3137
Bse118I RCCGGY 1 cut(s) 2049
Bse1I ACTGG 6 cut(s) 170, 423, 1636, 1946, 2349, 2432
Bse21I CCTNAGG 1 cut(s) 1319
Bse3DI GCAATG 2 cut(s) 1371, 2266
BseBI CCWGG 5 cut(s) 392, 662, 850, 1062, 2247
BseCI ATCGAT 1 cut(s) 33
BseDI CCNNGG 6 cut(s) 154, 237, 849, 1061, 2245, 2502
BseLI CCNNNNNNNGG 6 cut(s) 160, 1262, 1608, 2204, 2291, 3137
BseMI GCAATG 2 cut(s) 1371, 2266
BseNI ACTGG 6 cut(s) 170, 423, 1636, 1946, 2349, 2432
BseRI GAGGAG 1 cut(s) 3229
BseSI GKGCMC 1 cut(s) 3312
BseX3I CGGCCG 1 cut(s) 642
BseYI CCCAGC 1 cut(s) 1171
BsgI GTGCAG 4 cut(s) 1484, 1576, 2514, 2550
Bsh1285I CGRYCG 2 cut(s) 645, 2050
BshFI GGCC 7 cut(s) 284, 644, 767, 1132, 1650, 3107, 3381
BshNI GGYRCC 1 cut(s) 1367
BshTI ACCGGT 1 cut(s) 2049
BshVI ATCGAT 1 cut(s) 33
BsiEI CGRYCG 2 cut(s) 645, 2050
BsiHKAI GWGCWC 4 cut(s) 199, 387, 1729, 2880
BsiSI CCGG 7 cut(s) 299, 441, 971, 1129, 1885, 2050, 2359
BslFI GGGAC 3 cut(s) 148, 1956, 2963
BslI CCNNNNNNNGG 6 cut(s) 160, 1262, 1608, 2204, 2291, 3137
BsmAI GTCTC 8 cut(s) 41, 479, 643, 1554, 2486, 2511, 2888, 2998
BsmBI CGTCTC 2 cut(s) 41, 479
BsmFI GGGAC 3 cut(s) 148, 1956, 2963
BsmI GAATGC 4 cut(s) 719, 828, 933, 2888
BsnI GGCC 7 cut(s) 284, 644, 767, 1132, 1650, 3107, 3381
Bso31I GGTCTC 4 cut(s) 1554, 2486, 2888, 2998
Bsp1286I GDGCHC 6 cut(s) 199, 387, 1729, 2040, 2880, 3312
Bsp1407I TGTACA 2 cut(s) 1542, 3037
Bsp1720I GCTNAGC 3 cut(s) 92, 386, 782
Bsp19I CCATGG 2 cut(s) 154, 237
BspACI CCGC 9 cut(s) 101, 645, 665, 903, 921, 1256, 1376, 1844, 2905
BspANI GGCC 7 cut(s) 284, 644, 767, 1132, 1650, 3107, 3381
BspDI ATCGAT 1 cut(s) 33
BspHI TCATGA 2 cut(s) 927, 1951
BspLI GGNNCC 9 cut(s) 610, 946, 1350, 1369, 1600, 1972, 2037, 3380, 3487
BspMAI CTGCAG 1 cut(s) 1006
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 7 cut(s) 1247, 1308, 1414, 2324, 2915, 3480, 3493
BspT107I GGYRCC 1 cut(s) 1367
BspTNI GGTCTC 4 cut(s) 1554, 2486, 2888, 2998
BsrDI GCAATG 2 cut(s) 1371, 2266
BsrFI RCCGGY 1 cut(s) 2049
BsrGI TGTACA 2 cut(s) 1542, 3037
BsrI ACTGG 6 cut(s) 170, 423, 1636, 1946, 2349, 2432
BssAI RCCGGY 1 cut(s) 2049
BssECI CCNNGG 6 cut(s) 154, 237, 849, 1061, 2245, 2502
BssT1I CCWWGG 3 cut(s) 154, 237, 2502
Bst2UI CCWGG 5 cut(s) 392, 662, 850, 1062, 2247
Bst6I CTCTTC 3 cut(s) 2076, 3231, 3552
BstAPI GCANNNNNTGC 4 cut(s) 203, 1001, 1724, 3307
BstAUI TGTACA 2 cut(s) 1542, 3037
BstC8I GCNNGC 5 cut(s) 901, 1041, 1366, 1515, 3383
BstDSI CCRYGG 2 cut(s) 154, 237
BstHHI GCGC 3 cut(s) 970, 1607, 1618
BstMAI GTCTC 8 cut(s) 41, 479, 643, 1554, 2486, 2511, 2888, 2998
BstMCI CGRYCG 2 cut(s) 645, 2050
BstNI CCWGG 5 cut(s) 392, 662, 850, 1062, 2247
BstNSI RCATGY 4 cut(s) 979, 1933, 2419, 2726
BstSCI CCNGG 6 cut(s) 390, 660, 848, 1060, 1127, 2245
BstSFI CTRYAG 2 cut(s) 1002, 1185
BstSLI GKGCMC 1 cut(s) 3312
BstV2I GAAGAC 1 cut(s) 976
BstX2I RGATCY 4 cut(s) 1300, 1419, 2920, 3485
BstXI CCANNNNNNTGG 4 cut(s) 273, 398, 1643, 2474
BstYI RGATCY 4 cut(s) 1300, 1419, 2920, 3485
BstZI CGGCCG 1 cut(s) 642
Bsu15I ATCGAT 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 1319
BsuI GTATCC 1 cut(s) 2064
BsuRI GGCC 7 cut(s) 284, 644, 767, 1132, 1650, 3107, 3381
BsuTUI ATCGAT 1 cut(s) 33
BtgI CCRYGG 2 cut(s) 154, 237
BtrI CACGTC 1 cut(s) 356
BtsI GCAGTG 4 cut(s) 387, 566, 885, 1210
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 5 cut(s) 901, 1041, 1366, 1515, 3383
CaiI CAGNNNCTG 7 cut(s) 1010, 1209, 1493, 1742, 2118, 2528, 3512
CciI TCATGA 2 cut(s) 927, 1951
CfoI GCGC 3 cut(s) 970, 1607, 1618
Cfr10I RCCGGY 1 cut(s) 2049
Cfr13I GGNCC 4 cut(s) 1970, 3074, 3106, 3379
ClaI ATCGAT 1 cut(s) 33
CseI GACGC 1 cut(s) 2577
Csp6I GTAC 7 cut(s) 277, 1221, 1480, 1543, 2740, 2819, 3038
CspAI ACCGGT 1 cut(s) 2049
CviQI GTAC 7 cut(s) 277, 1221, 1480, 1543, 2740, 2819, 3038
DrdI GACNNNNNNGTC 1 cut(s) 980
DriI GACNNNNNGTC 2 cut(s) 696, 2362
DseDI GACNNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 2 cut(s) 642, 765
EagI CGGCCG 1 cut(s) 642
Eam1104I CTCTTC 3 cut(s) 2076, 3231, 3552
Eam1105I GACNNNNNGTC 2 cut(s) 696, 2362
EarI CTCTTC 3 cut(s) 2076, 3231, 3552
EciI GGCGGA 2 cut(s) 680, 1245
EclXI CGGCCG 1 cut(s) 642
Eco130I CCWWGG 3 cut(s) 154, 237, 2502
Eco24I GRGCYC 1 cut(s) 2040
Eco31I GGTCTC 4 cut(s) 1554, 2486, 2888, 2998
Eco32I GATATC 2 cut(s) 179, 3445
Eco47I GGWCC 2 cut(s) 1970, 3074
Eco52I CGGCCG 1 cut(s) 642
Eco57I CTGAAG 5 cut(s) 1032, 1998, 2095, 3016, 3081
Eco81I CCTNAGG 1 cut(s) 1319
EcoO109I RGGNCCY 1 cut(s) 3379
EcoRII CCWGG 5 cut(s) 390, 660, 848, 1060, 2245
EcoRV GATATC 2 cut(s) 179, 3445
EcoT14I CCWWGG 3 cut(s) 154, 237, 2502
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 2040
ErhI CCWWGG 3 cut(s) 154, 237, 2502
Esp3I CGTCTC 2 cut(s) 41, 479
FalI AAGNNNNNCTT 8 cut(s) 1749, 1781, 2186, 2218, 3210, 3242, 3297, 3329
FaqI GGGAC 3 cut(s) 148, 1956, 2963
FauNDI CATATG 2 cut(s) 268, 695
FbaI TGATCA 2 cut(s) 435, 1026
FriOI GRGCYC 1 cut(s) 2040
FspBI CTAG 5 cut(s) 365, 459, 648, 800, 1235
GlaI GCGC 3 cut(s) 969, 1606, 1617
GsaI CCCAGC 1 cut(s) 1175
GsuI CTGGAG 2 cut(s) 1665, 2449
HaeIII GGCC 7 cut(s) 284, 644, 767, 1132, 1650, 3107, 3381
HapII CCGG 7 cut(s) 299, 441, 971, 1129, 1885, 2050, 2359
HgaI GACGC 1 cut(s) 2577
HhaI GCGC 3 cut(s) 970, 1607, 1618
Hin6I GCGC 3 cut(s) 968, 1605, 1616
HinP1I GCGC 3 cut(s) 968, 1605, 1616
HincII GTYRAC 1 cut(s) 2425
HindII GTYRAC 1 cut(s) 2425
HpaII CCGG 7 cut(s) 299, 441, 971, 1129, 1885, 2050, 2359
HphI GGTGA 8 cut(s) 430, 1036, 1050, 1874, 2001, 2299, 3352, 3479
Hpy166II GTNNAC 6 cut(s) 1111, 1543, 2425, 2742, 2841, 3038
Hpy8I GTNNAC 6 cut(s) 1111, 1543, 2425, 2742, 2841, 3038
Hpy99I CGWCG 1 cut(s) 2593
HpyAV CCTTC 9 cut(s) 295, 850, 1060, 1134, 1157, 1741, 1779, 2832, 3119
HpyCH4IV ACGT 5 cut(s) 71, 137, 355, 488, 2982
HpySE526I ACGT 5 cut(s) 71, 137, 355, 488, 2982
HspAI GCGC 3 cut(s) 968, 1605, 1616
Ksp22I TGATCA 2 cut(s) 435, 1026
LmnI GCTCC 9 cut(s) 608, 944, 1334, 1530, 1887, 2035, 3083, 3242, 3298
MaeI CTAG 5 cut(s) 365, 459, 648, 800, 1235
MaeII ACGT 5 cut(s) 71, 137, 355, 488, 2982
MaeIII GTNAC 5 cut(s) 449, 835, 883, 1237, 3013
MflI RGATCY 4 cut(s) 1300, 1419, 2920, 3485
MhlI GDGCHC 6 cut(s) 199, 387, 1729, 2040, 2880, 3312
MlsI TGGCCA 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 767
MlyI GAGTC 5 cut(s) 1641, 1727, 1964, 2513, 2570
MmeI TCCRAC 4 cut(s) 154, 298, 706, 3420
Mox20I TGGCCA 1 cut(s) 767
Mph1103I ATGCAT 1 cut(s) 721
MroXI GAANNNNTTC 2 cut(s) 3029, 3094
MscI TGGCCA 1 cut(s) 767
MseI TTAA 6 cut(s) 243, 419, 1827, 1926, 2340, 2991
MslI CAYNNNNRTG 5 cut(s) 271, 1506, 2247, 2333, 2873
Msp20I TGGCCA 1 cut(s) 767
MspA1I CMGCKG 2 cut(s) 923, 2525
MspI CCGG 7 cut(s) 299, 441, 971, 1129, 1885, 2050, 2359
MspR9I CCNGG 6 cut(s) 392, 662, 850, 1062, 1129, 2247
Mva1269I GAATGC 4 cut(s) 719, 828, 933, 2888
MvaI CCWGG 5 cut(s) 392, 662, 850, 1062, 2247
NciI CCSGG 1 cut(s) 1129
NcoI CCATGG 2 cut(s) 154, 237
NdeI CATATG 2 cut(s) 268, 695
NlaIV GGNNCC 9 cut(s) 610, 946, 1350, 1369, 1600, 1972, 2037, 3380, 3487
NmeAIII GCCGAG 3 cut(s) 168, 620, 716
NmuCI GTSAC 3 cut(s) 835, 883, 1237
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 4 cut(s) 979, 1933, 2419, 2726
OliI CACNNNNGTG 1 cut(s) 2247
PagI TCATGA 2 cut(s) 927, 1951
PaqCI CACCTGC 1 cut(s) 241
PctI GAATGC 4 cut(s) 719, 828, 933, 2888
PdmI GAANNNNTTC 2 cut(s) 3029, 3094
PflMI CCANNNNNTGG 1 cut(s) 160
PinAI ACCGGT 1 cut(s) 2049
PleI GAGTC 5 cut(s) 1640, 1727, 1963, 2513, 2569
PpsI GAGTC 5 cut(s) 1640, 1727, 1963, 2513, 2569
PsiI TTATAA 1 cut(s) 2219
Psp1406I AACGTT 2 cut(s) 71, 2982
Psp6I CCWGG 5 cut(s) 390, 660, 848, 1060, 2245
PspFI CCCAGC 1 cut(s) 1171
PspGI CCWGG 5 cut(s) 390, 660, 848, 1060, 2245
PspN4I GGNNCC 9 cut(s) 610, 946, 1350, 1369, 1600, 1972, 2037, 3380, 3487
PspPI GGNCC 4 cut(s) 1970, 3074, 3106, 3379
PstI CTGCAG 1 cut(s) 1006
PstNI CAGNNNCTG 7 cut(s) 1010, 1209, 1493, 1742, 2118, 2528, 3512
PsuI RGATCY 4 cut(s) 1300, 1419, 2920, 3485
PvuII CAGCTG 1 cut(s) 2525
RsaI GTAC 7 cut(s) 278, 1222, 1481, 1544, 2741, 2820, 3039
RsaNI GTAC 7 cut(s) 277, 1221, 1480, 1543, 2740, 2819, 3038
RseI CAYNNNNRTG 5 cut(s) 271, 1506, 2247, 2333, 2873
SaqAI TTAA 6 cut(s) 243, 419, 1827, 1926, 2340, 2991
Sau96I GGNCC 4 cut(s) 1970, 3074, 3106, 3379
ScaI AGTACT 2 cut(s) 1222, 1481
SchI GAGTC 5 cut(s) 1641, 1727, 1964, 2513, 2570
ScrFI CCNGG 6 cut(s) 392, 662, 850, 1062, 1129, 2247
SduI GDGCHC 6 cut(s) 199, 387, 1729, 2040, 2880, 3312
SfcI CTRYAG 2 cut(s) 1002, 1185
SinI GGWCC 2 cut(s) 1970, 3074
SmiMI CAYNNNNRTG 5 cut(s) 271, 1506, 2247, 2333, 2873
SmlI CTYRAG 2 cut(s) 1685, 2311
SmoI CTYRAG 2 cut(s) 1685, 2311
SpeI ACTAGT 2 cut(s) 458, 1234
SsiI CCGC 9 cut(s) 101, 645, 665, 903, 921, 1256, 1376, 1844, 2905
SspI AATATT 1 cut(s) 2716
SspMI CTAG 5 cut(s) 365, 459, 648, 800, 1235
StyD4I CCNGG 6 cut(s) 390, 660, 848, 1060, 1127, 2245
StyI CCWWGG 3 cut(s) 154, 237, 2502
TaiI ACGT 5 cut(s) 74, 140, 358, 491, 2985
TaqI TCGA 6 cut(s) 33, 201, 408, 1958, 2771, 2892
TaqII GACCGA 1 cut(s) 3062
TatI WGTACW 4 cut(s) 1220, 1479, 1542, 3037
TauI GCSGC 2 cut(s) 647, 923
Tru1I TTAA 6 cut(s) 243, 419, 1827, 1926, 2340, 2991
Tru9I TTAA 6 cut(s) 243, 419, 1827, 1926, 2340, 2991
TseFI GTSAC 3 cut(s) 835, 883, 1237
Tsp45I GTSAC 3 cut(s) 835, 883, 1237
TspGWI ACGGA 2 cut(s) 120, 1635
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 2 cut(s) 1970, 3074
XapI RAATTY 5 cut(s) 1313, 1575, 1907, 2972, 3361
XceI RCATGY 4 cut(s) 979, 1933, 2419, 2726
XcmI CCANNNNNNNNNTGG 1 cut(s) 2474
XmnI GAANNNNTTC 2 cut(s) 3029, 3094
XspI CTAG 5 cut(s) 365, 459, 648, 800, 1235
ZrmI AGTACT 2 cut(s) 1222, 1481
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.