Rroxscaffold_4G00286200

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009831
Physical Location & Seq
Forward (+)
7324648 .. 7328501
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286200.1

Sequence Viewer

Length: 1965 bp
ATGAAGGATCAGTGGAGGTTCAATCGGTTTAGAGACCCTTTGGGGGTTGTGGGTGTGGTGGTTGTGTGGTTGCTGTTTCAGAATCTGAGCTTGTGTTGGTCTTTGAATGATGAAGGTTTGGCGCTTTTGAGGTTTAGAGAGAGAGTAGTGAGTGACCCATTTGGTGCTTTGTCGAACTGGAATGATGAAGATGGAGAGATTGACCCTTGCTCTTGGTTTGGGGTTGAATGCTCAGAGGGAAAAGTTGTGGTCTTGAACTTGAAAGATCTTTGTCTTGGAGGAACGTTATCACCTGAGCTCGGAAACCTTGTTCATATAAAGTCTATTATTTTGCGCAACAACTCCTTTATGGGGATAATTCCTGGAGGGATCGGAGAGTTGAAGGAACTGGAGGTGTTAGACTTGGGATATAATAATTTCAGTGGAACACTTCCAGTAGACCTTGACAGTAATTTTTCATTGAGAATCCTGCTACTAGATAACAACAGGCTCCTTAGTATCTTATCTCCGGAGATTTATGCACTGGAGATGCTTTCCCAATTTCAAGTCGACGAAAACCAACTTTCTGGTGCAACCAGAGAATCATCGTGCAATGAAAGATCCATTTCAAGGAACCTTGCTAATACTGAACACTCCTATCATGGGAGACAGCTGGGAGCAGCATCCCCTCCAAATATTCCTAGCTTGAATCCTCCCAACGAACCACCAAAGACAGAACCATCTGCTCCTACACCTGCACCTGCATCTTCACCTTCACCTTCACCTTCACCTTCTGTTCCAACTTCCCCACCAACCACATCTGCTTCTCCACCTTCAGTTTCCTCTCCTGGGCCTTCCCCCCAAGTAAGTTCCAGTCATCGTGTTGCAATAATGGCTGGAGCCATAGGCGGTGGTGGTTTTCTTGTAATTTTAGTTATTGGGTTGTATTTATATGGACGCAACAAGGTAGCTACTGTCAAACCTTGGGCCACAGGATTAAGCGGGCAGCTTCAGAAAGCCTTTGTAACTGGTATTGCTTCTTTCGAGATTCTCATTGCAGGTGTACCAAATCTTAAGAGATCGGAGATTCAAGCGGCTTGTGAAGATTTCAGTAATGTTATAGGTACTTCTTCACCGATGGGTACAGTGTACAAAGGAACATTATCCAGTGGTGTCGAAATTGCTGTGGCTTCTCTCGCAGCAACATCTGCCAGTGGTTGGGGCAACAATTTAGAGTTGCAGTTTAGGAAGAAGATCGACACATTATCGAAAGTGAACCACAAGAATTTTGTCAGCCTTATTGGATATTGCGAGGAAGAAGAGCCTTTCACTAGAATGATGGTTTTTGAATATGCTCCCAATGGCACACTTTTCGAGCATTTACACATAAAAGAAGCTGAGCACCTGGACTGGAGAATGCGAATGAGAATCTCAATGGGAATGGCCTACTGCCTTGAGCATATGCATCAGCTGAAGCCGCCTATAGTTCACAACAACTTGACTTCTTCAGCTGTCCAGCTAACGGAGGATTATGCACCGAAGGTCTCGGAAGTCACTTTCTGGAATGAGATAGCTGAAGCTGAGAAACAATCATCTGGCTCAAGACTTTCTGATGCACAATCAACAAGCCTGGAAAGTAATGTGTACAGTTTTGGTGTAATTTTGTTTGAAATCGTGACAGGTAGACTCCCTTATTCAGTAGACAATGACTCACTTGAAGACTGGGCTTCTGACTATTTGAGAGGGGAGCATCCCTTCAAGGGAATGGTTGATCCAACTCTAGAATCTTTCCAAGAGGAGCAGCTGGAGCGAATTGGAGAAGTAATAAGAATTTGTGTCCATCCTGACCCGAGACAAAGACCGCCAATGAGAGACGTCACTGCAAGGCTGAGAGAGATAACTGGACTTACACCTGATGCAGTATCCCCGAAACTCTCCACTCTTTGGTGGGCAGAGCTTGAACTTATGTCTGCAGATGCAAGCTAG

Protein Analysis

654

Amino Acids

71.67

Weight (kDa)

5.05

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 78 1.1e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 105 - 164 2.8e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 372 - 623 5.8e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 373 - 619 2.1e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009831)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40270 AT2G40270 AT3G56050 AT3G56050
fragaria_vesca FvH4_7g24950 FvH4_7g24950 FvH4_7g24950
malus_domestica MD01G1156900.v1.1
prunus_persica Prupe.2G254500_v2.0.a1
pyrus_communis pycom01g17370
rosa_chinensis RchiOBHm_Chr1g0370831
rosa_laevigata RLG00000027005
rosa_multiflora Rmu_sc0009474.1_g000013
rosa_roxburghii Rroxscaffold_4G00286200
rosa_rugosa Rorug01G0359900
rosa_samantha Rh1AG369400 Rh1BG332800 Rh1BG332900 Rh1CG346500 Rh1DG364100
rosa_wichuraiana Rw1G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 742, 748, 1028
AatII GACGTC 1 cut(s) 1857
Acc16I TGCGCA 1 cut(s) 335
Acc36I ACCTGC 3 cut(s) 742, 748, 1028
AccB7I CCANNNNNTGG 2 cut(s) 1197, 1923
AccI GTMKAC 4 cut(s) 438, 549, 1663, 1680
AccIII TCCGGA 1 cut(s) 508
AciI CCGC 5 cut(s) 888, 981, 1073, 1457, 1841
AclI AACGTT 1 cut(s) 284
AclWI GGATC 4 cut(s) 15, 377, 594, 1745
AcsI RAATTY 2 cut(s) 1264, 1809
AcuI CTGAAG 5 cut(s) 798, 974, 1470, 1472, 1575
AcyI GRCGYC 1 cut(s) 1854
AfaI GTAC 5 cut(s) 1044, 1105, 1123, 1130, 1625
AflII CTTAAG 1 cut(s) 1052
AjnI CCWGG 4 cut(s) 361, 826, 1383, 1608
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
Alw21I GWGCWC 2 cut(s) 300, 1383
Alw26I GTCTC 5 cut(s) 27, 640, 1528, 1825, 1845
AlwI GGATC 4 cut(s) 15, 377, 594, 1745
AlwNI CAGNNNCTG 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 1828
Aor13HI TCCGGA 1 cut(s) 508
AoxI GGCC 3 cut(s) 830, 966, 1422
ApeKI GCWGC 4 cut(s) 659, 985, 1178, 1780
ApoI RAATTY 2 cut(s) 1264, 1809
AspLEI GCGC 2 cut(s) 124, 336
AspS9I GGNCC 2 cut(s) 830, 966
AsuHPI GGTGA 6 cut(s) 282, 741, 747, 753, 759, 1104
AvaI CYCGRG 1 cut(s) 1828
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 1704
Bbv12I GWGCWC 2 cut(s) 300, 1383
BbvI GCAGC 4 cut(s) 671, 997, 1190, 1792
BccI CCATC 5 cut(s) 185, 727, 1111, 1312, 1827
BcgI CGANNNNNNTGC 2 cut(s) 1333, 1367
BciT130I CCWGG 4 cut(s) 363, 828, 1385, 1610
BciVI GTATCC 1 cut(s) 1912
BcoDI GTCTC 5 cut(s) 27, 640, 1528, 1825, 1845
BfaI CTAG 5 cut(s) 476, 681, 1311, 1760, 1963
BfmI CTRYAG 2 cut(s) 1461, 1950
BfoI RGCGCY 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 1052
BfuAI ACCTGC 3 cut(s) 742, 748, 1028
BfuI GTATCC 1 cut(s) 1912
BglII AGATCT 1 cut(s) 265
BisI GCNGC 6 cut(s) 660, 986, 1074, 1179, 1457, 1781
BlpI GCTNAGC 1 cut(s) 1377
BlsI GCNGC 6 cut(s) 661, 987, 1075, 1180, 1458, 1782
Bme1390I CCNGG 4 cut(s) 363, 828, 1385, 1610
BmeT110I CYCGRG 1 cut(s) 1828
BmgT120I GGNCC 2 cut(s) 830, 966
BmiI GGNNCC 3 cut(s) 491, 614, 880
BmrFI CCNGG 4 cut(s) 363, 828, 1385, 1610
BmrI ACTGGG 1 cut(s) 1711
BmsI GCATC 8 cut(s) 519, 671, 752, 1453, 1582, 1738, 1885, 1945
BmuI ACTGGG 1 cut(s) 1711
BpiI GAAGAC 1 cut(s) 1704
BpmI CTGGAG 6 cut(s) 384, 410, 545, 897, 1411, 1805
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 1377
BpuEI CTTGAG 2 cut(s) 1454, 1564
BsaHI GRCGYC 1 cut(s) 1854
BsaI GGTCTC 2 cut(s) 27, 1528
BsaJI CCNNGG 2 cut(s) 827, 962
BsaWI WCCGGW 1 cut(s) 508
Bse3DI GCAATG 2 cut(s) 598, 1032
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 4 cut(s) 363, 828, 1385, 1610
BseDI CCNNGG 2 cut(s) 827, 962
BseGI GGATG 3 cut(s) 662, 1729, 1819
BseMI GCAATG 2 cut(s) 598, 1032
BseMII CTCAG 6 cut(s) 77, 246, 285, 1368, 1551, 1859
BseRI GAGGAG 1 cut(s) 1790
BseXI GCAGC 4 cut(s) 671, 997, 1190, 1792
BseYI CCCAGC 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 3 cut(s) 832, 968, 1424
BsiHKAI GWGCWC 2 cut(s) 300, 1383
BsiHKCI CYCGRG 1 cut(s) 1828
BsiSI CCGG 1 cut(s) 509
BsmAI GTCTC 5 cut(s) 27, 640, 1528, 1825, 1845
BsmBI CGTCTC 1 cut(s) 1845
BsmI GAATGC 2 cut(s) 233, 1401
BsnI GGCC 3 cut(s) 832, 968, 1424
Bso31I GGTCTC 2 cut(s) 27, 1528
BsoBI CYCGRG 1 cut(s) 1828
Bsp1286I GDGCHC 2 cut(s) 300, 1383
Bsp13I TCCGGA 1 cut(s) 508
Bsp1407I TGTACA 2 cut(s) 1128, 1623
Bsp143I GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
Bsp1720I GCTNAGC 1 cut(s) 1377
BspACI CCGC 5 cut(s) 888, 981, 1073, 1457, 1841
BspANI GGCC 3 cut(s) 832, 968, 1424
BspCNI CTCAG 6 cut(s) 78, 245, 286, 1369, 1552, 1860
BspEI TCCGGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 491, 614, 880
BspMAI CTGCAG 1 cut(s) 1954
BspMI ACCTGC 3 cut(s) 742, 748, 1028
BspPI GGATC 4 cut(s) 15, 377, 594, 1745
BspQI GCTCTTC 1 cut(s) 1293
BspTI CTTAAG 1 cut(s) 1052
BspTNI GGTCTC 2 cut(s) 27, 1528
BsrDI GCAATG 2 cut(s) 598, 1032
BsrGI TGTACA 2 cut(s) 1128, 1623
BssECI CCNNGG 2 cut(s) 827, 962
BssMI GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
BssNI GRCGYC 1 cut(s) 1854
BssT1I CCWWGG 1 cut(s) 962
Bst2UI CCWGG 4 cut(s) 363, 828, 1385, 1610
Bst4CI ACNGT 4 cut(s) 449, 955, 1126, 1628
Bst6I CTCTTC 1 cut(s) 1293
BstACI GRCGYC 1 cut(s) 1854
BstAFI CTTAAG 1 cut(s) 1052
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstAUI TGTACA 2 cut(s) 1128, 1623
BstC8I GCNNGC 2 cut(s) 983, 1960
BstDEI CTNAG 7 cut(s) 86, 232, 294, 494, 1377, 1560, 1868
BstF5I GGATG 3 cut(s) 662, 1729, 1819
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 336
BstKTI GATC 7 cut(s) 10, 268, 372, 602, 1061, 1236, 1753
BstMAI GTCTC 5 cut(s) 27, 640, 1528, 1825, 1845
BstMBI GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
BstMWI GCNNNNNNNGC 5 cut(s) 872, 1175, 1187, 1456, 1786
BstNI CCWGG 4 cut(s) 363, 828, 1385, 1610
BstSCI CCNGG 4 cut(s) 361, 826, 1383, 1608
BstSFI CTRYAG 2 cut(s) 1461, 1950
BstV1I GCAGC 4 cut(s) 671, 997, 1190, 1792
BstV2I GAAGAC 1 cut(s) 1704
BstX2I RGATCY 2 cut(s) 265, 599
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 2 cut(s) 265, 599
BsuI GTATCC 1 cut(s) 1912
BsuRI GGCC 3 cut(s) 832, 968, 1424
BtsCI GGATG 3 cut(s) 662, 1729, 1819
BtsI GCAGTG 1 cut(s) 1857
BtsIMutI CAGTG 7 cut(s) 17, 427, 521, 1131, 1153, 1198, 1857
BveI ACCTGC 3 cut(s) 742, 748, 1028
Cac8I GCNNGC 2 cut(s) 983, 1960
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 2 cut(s) 124, 336
Cfr13I GGNCC 2 cut(s) 830, 966
CseI GACGC 1 cut(s) 945
Csp6I GTAC 5 cut(s) 1043, 1104, 1122, 1129, 1624
CviAII CATG 1 cut(s) 641
CviQI GTAC 5 cut(s) 1043, 1104, 1122, 1129, 1624
DdeI CTNAG 7 cut(s) 86, 232, 294, 494, 1377, 1560, 1868
DpnI GATC 7 cut(s) 9, 267, 371, 601, 1060, 1235, 1752
DpnII GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
Eam1104I CTCTTC 1 cut(s) 1293
EarI CTCTTC 1 cut(s) 1293
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 1 cut(s) 300
Eco31I GGTCTC 2 cut(s) 27, 1528
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 5 cut(s) 798, 974, 1470, 1472, 1575
Eco88I CYCGRG 1 cut(s) 1828
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 4 cut(s) 361, 826, 1383, 1608
EcoT14I CCWWGG 1 cut(s) 962
EcoT22I ATGCAT 1 cut(s) 1446
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 962
Esp3I CGTCTC 1 cut(s) 1845
FaeI CATG 1 cut(s) 644
FatI CATG 1 cut(s) 640
FauI CCCGC 1 cut(s) 974
FauNDI CATATG 1 cut(s) 1440
FblI GTMKAC 4 cut(s) 438, 549, 1663, 1680
Fnu4HI GCNGC 6 cut(s) 660, 986, 1074, 1179, 1457, 1781
FokI GGATG 3 cut(s) 649, 1716, 1806
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 660, 986, 1074, 1179, 1457, 1781
FspBI CTAG 5 cut(s) 476, 681, 1311, 1760, 1963
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 2 cut(s) 123, 335
GluI GCNGC 6 cut(s) 660, 986, 1074, 1179, 1457, 1781
GsaI CCCAGC 1 cut(s) 656
GsuI CTGGAG 6 cut(s) 384, 410, 545, 897, 1411, 1805
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 832, 968, 1424
HapII CCGG 1 cut(s) 509
HgaI GACGC 1 cut(s) 945
HhaI GCGC 2 cut(s) 124, 336
Hin1I GRCGYC 1 cut(s) 1854
Hin1II CATG 1 cut(s) 644
Hin6I GCGC 2 cut(s) 122, 334
HinP1I GCGC 2 cut(s) 122, 334
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HpaII CCGG 1 cut(s) 509
HphI GGTGA 6 cut(s) 282, 741, 747, 753, 759, 1104
Hpy166II GTNNAC 9 cut(s) 439, 550, 1043, 1129, 1255, 1468, 1624, 1664, 1681
Hpy188III TCNNGA 8 cut(s) 253, 509, 1024, 1540, 1581, 1654, 1760, 1823
Hpy8I GTNNAC 9 cut(s) 439, 550, 1043, 1129, 1255, 1468, 1624, 1664, 1681
Hpy99I CGWCG 1 cut(s) 554
HpyCH4III ACNGT 4 cut(s) 449, 955, 1126, 1628
HpyCH4IV ACGT 2 cut(s) 284, 1854
HpyF10VI GCNNNNNNNGC 5 cut(s) 872, 1175, 1187, 1456, 1786
HpyF3I CTNAG 7 cut(s) 86, 232, 294, 494, 1377, 1560, 1868
HpySE526I ACGT 2 cut(s) 284, 1854
Hsp92I GRCGYC 1 cut(s) 1854
Hsp92II CATG 1 cut(s) 644
HspAI GCGC 2 cut(s) 122, 334
Kpn2I TCCGGA 1 cut(s) 508
Kzo9I GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
LguI GCTCTTC 1 cut(s) 1293
LmnI GCTCC 8 cut(s) 495, 656, 730, 878, 1339, 1726, 1777, 1786
Lsp1109I GCAGC 4 cut(s) 671, 997, 1190, 1792
LweI GCATC 8 cut(s) 519, 671, 752, 1453, 1582, 1738, 1885, 1945
MaeI CTAG 5 cut(s) 476, 681, 1311, 1760, 1963
MaeII ACGT 2 cut(s) 284, 1854
MaeIII GTNAC 5 cut(s) 152, 1003, 1531, 1654, 1855
MalI GATC 7 cut(s) 9, 267, 371, 601, 1060, 1235, 1752
MboI GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
MflI RGATCY 2 cut(s) 265, 599
MhlI GDGCHC 2 cut(s) 300, 1383
MlyI GAGTC 2 cut(s) 1659, 1682
MmeI TCCRAC 2 cut(s) 803, 1778
Mph1103I ATGCAT 1 cut(s) 1446
MroI TCCGGA 1 cut(s) 508
MseI TTAA 2 cut(s) 977, 1053
MslI CAYNNNNRTG 1 cut(s) 1313
MspA1I CMGCKG 4 cut(s) 652, 1450, 1490, 1783
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 4 cut(s) 363, 828, 1385, 1610
Mva1269I GAATGC 2 cut(s) 233, 1401
MvaI CCWGG 4 cut(s) 363, 828, 1385, 1610
MwoI GCNNNNNNNGC 5 cut(s) 872, 1175, 1187, 1456, 1786
NdeI CATATG 1 cut(s) 1440
NdeII GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
NlaIII CATG 1 cut(s) 644
NlaIV GGNNCC 3 cut(s) 491, 614, 880
NmuCI GTSAC 4 cut(s) 152, 1531, 1654, 1855
NsbI TGCGCA 1 cut(s) 335
NsiI ATGCAT 1 cut(s) 1446
PaqCI CACCTGC 3 cut(s) 742, 748, 1028
PciSI GCTCTTC 1 cut(s) 1293
PctI GAATGC 2 cut(s) 233, 1401
PfeI GAWTC 8 cut(s) 82, 465, 581, 688, 1027, 1066, 1407, 1763
PflMI CCANNNNNTGG 2 cut(s) 1197, 1923
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 6 cut(s) 661, 987, 1075, 1180, 1458, 1782
PleI GAGTC 2 cut(s) 1659, 1682
PpsI GAGTC 2 cut(s) 1659, 1682
Psp124BI GAGCTC 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 284
Psp6I CCWGG 4 cut(s) 361, 826, 1383, 1608
PspFI CCCAGC 1 cut(s) 652
PspGI CCWGG 4 cut(s) 361, 826, 1383, 1608
PspN4I GGNNCC 3 cut(s) 491, 614, 880
PspPI GGNCC 2 cut(s) 830, 966
PstI CTGCAG 1 cut(s) 1954
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 265, 599
PvuII CAGCTG 4 cut(s) 652, 1450, 1490, 1783
RsaI GTAC 5 cut(s) 1044, 1105, 1123, 1130, 1625
RsaNI GTAC 5 cut(s) 1043, 1104, 1122, 1129, 1624
RseI CAYNNNNRTG 1 cut(s) 1313
SacI GAGCTC 1 cut(s) 300
SalI GTCGAC 1 cut(s) 548
SapI GCTCTTC 1 cut(s) 1293
SaqAI TTAA 2 cut(s) 977, 1053
SatI GCNGC 6 cut(s) 660, 986, 1074, 1179, 1457, 1781
Sau3AI GATC 7 cut(s) 7, 265, 369, 599, 1058, 1233, 1750
Sau96I GGNCC 2 cut(s) 830, 966
SchI GAGTC 2 cut(s) 1659, 1682
ScrFI CCNGG 4 cut(s) 363, 828, 1385, 1610
SduI GDGCHC 2 cut(s) 300, 1383
SfaNI GCATC 8 cut(s) 519, 671, 752, 1453, 1582, 1738, 1885, 1945
SfcI CTRYAG 2 cut(s) 1461, 1950
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 3 cut(s) 1052, 1433, 1579
SmoI CTYRAG 3 cut(s) 1052, 1433, 1579
SsiI CCGC 5 cut(s) 888, 981, 1073, 1457, 1841
SspI AATATT 1 cut(s) 676
SspMI CTAG 5 cut(s) 476, 681, 1311, 1760, 1963
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 4 cut(s) 361, 826, 1383, 1608
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 4 cut(s) 449, 955, 1126, 1628
TaiI ACGT 2 cut(s) 287, 1857
TaqI TCGA 7 cut(s) 173, 549, 1023, 1155, 1236, 1247, 1353
TatI WGTACW 2 cut(s) 1128, 1623
TauI GCSGC 2 cut(s) 1076, 1459
TfiI GAWTC 8 cut(s) 82, 465, 581, 688, 1027, 1066, 1407, 1763
Tru1I TTAA 2 cut(s) 977, 1053
Tru9I TTAA 2 cut(s) 977, 1053
TscAI CASTG 7 cut(s) 17, 427, 528, 1131, 1153, 1198, 1864
TseFI GTSAC 4 cut(s) 152, 1531, 1654, 1855
TseI GCWGC 4 cut(s) 659, 985, 1178, 1780
Tsp45I GTSAC 4 cut(s) 152, 1531, 1654, 1855
TspDTI ATGAA 6 cut(s) 17, 126, 201, 302, 447, 609
TspGWI ACGGA 1 cut(s) 1517
TspRI CASTG 7 cut(s) 17, 427, 528, 1131, 1153, 1198, 1864
Van91I CCANNNNNTGG 2 cut(s) 1197, 1923
Vha464I CTTAAG 1 cut(s) 1052
XapI RAATTY 2 cut(s) 1264, 1809
XbaI TCTAGA 1 cut(s) 1759
XmiI GTMKAC 4 cut(s) 438, 549, 1663, 1680
XspI CTAG 5 cut(s) 476, 681, 1311, 1760, 1963
ZraI GACGTC 1 cut(s) 1855
Zsp2I ATGCAT 1 cut(s) 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.