Rroxscaffold_4G00302940

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015688
Physical Location & Seq
Forward (+)
23266934 .. 23273140
6207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302940.1

Sequence Viewer

Length: 1350 bp
ATGTTCAGATCTTCCCTTCCAAAACTCTTAGAACTCCCACCACCTCAGATCGACTTCGGCTTGGACTTCCGTATTATAGAAAGTGTCGGTGGCCGTGTGTGCAAGGCAATACTTGATTCTGGAAGAATTATTGCTGTTAAAAAGGTGGAGTACTTTTGTATTGCTTTTGTGCTGTCATGGCCTTTGTTCTTGTTGAGTTCATATGGATTGTTATTTTGTGTGGATTTGGCGAGGAGTTGCTTCCTCCTTGTAAAGGTCATGGCCAAATTGAATACTCAAATTCAAATTCCAGAAGATGGTTCAAATGAGAACCAGATTAAGAAGCTGGAAGATGAAGTGAGGAATCTACAATTGCTGTCTCATCCAAATATTGTTCAATATTTGGGAACGAGGAGAGTGGGGGGATCTTTGTGTATCCTGATGGAATTCTGGGGCAAATCAGACTGCTCGAAGGAACTAAGACTGTTCAACGAGACAAAAACAAGAAGATTCACCATTCAGTTGTTGCAGGGACTACAATATTTACATGAACGTAAAATCGTGCACGGGAATATTAAGGGAAGAAATATCCTGGTTGATGCTTTTGGAGACATTAAGCTTGCTGATTTTGGTCTATCAGCACATTCAAACCTTTTGGGCCCTCAGTCTTCATCTGGATACAGACATTCTGTTGAAATCTTTCAACCCCCGCCTCATCTCGACCCATTTGCTGATATATGGTGTGTTGGGTGCATTATGATTGAGCTGACCACCGGAAAGGATCCATGGCGAAGAGAGAGCTGGTCGAATTATATTTTGCCTAAGAAATTTTCTTTTCATGCGAAAAACTTTTTGCAAGAGTGTCTTCAATGGGAACAACAAGATTCGAGGCTACCCACATTTGTCTTGCTTCAGCATCCATTCTTAAATCCTGCATCAATTGAGGTCCGACCTACTCAACTACTTCCGAGGGAAAGCTTAGTTGCTGAAGTGCTATATTGTACCTCACCTTCACCAACTTACGCAGCTGTTGGTGGAGATCATTGTAGATTGATGCTAGATCAGAATAGCATTTCAGGAGATGAGGGAGCTGCTGAACCTTCTCATACTGCTGAACCTCCTATAATGCAGAGACCTAACCAAGCCTTTGCACTTCAACATGGTGCCGGAGCTCCTGTCAATTTAACTCTTAGTTCCAATGTTGGATCAACTCCCAGTACTTGTCCTAATATTAAGTCCAATACTTCCGACTCCAAATCTATTTCGACACTTGTTCATATTCTCGATCCAAATAGTACGTGCTTCCAAAAGGAGGCGTACGGTAATTTCAAGAGCTTTCCAGGGGATAAAGAATTTTTATTGAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

449

Amino Acids

50.28

Weight (kDa)

7.55

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 93 - 258 2.5e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 100 - 302 5.6e-33 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1142
AccB7I CCANNNNNTGG 1 cut(s) 296
AciI CCGC 1 cut(s) 689
AclWI GGATC 5 cut(s) 412, 755, 768, 1192, 1259
AcoI YGGCCR 2 cut(s) 91, 261
AcsI RAATTY 5 cut(s) 279, 285, 425, 806, 1331
AcuI CTGAAG 2 cut(s) 875, 987
AfaI GTAC 5 cut(s) 152, 982, 1198, 1276, 1298
AfiI CCNNNNNNNGG 3 cut(s) 253, 296, 1291
AjnI CCWGG 2 cut(s) 570, 1318
AluBI AGCT 9 cut(s) 325, 598, 745, 780, 957, 1007, 1070, 1151, 1314
AluI AGCT 9 cut(s) 325, 598, 745, 780, 957, 1007, 1070, 1151, 1314
Alw21I GWGCWC 2 cut(s) 546, 1153
Alw26I GTCTC 4 cut(s) 363, 467, 582, 1105
Alw44I GTGCAC 1 cut(s) 542
AlwI GGATC 5 cut(s) 412, 755, 768, 1192, 1259
AoxI GGCC 4 cut(s) 91, 179, 261, 637
ApaI GGGCCC 1 cut(s) 641
ApaLI GTGCAC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 1004, 1070
ApoI RAATTY 5 cut(s) 279, 285, 425, 806, 1331
Asp700I GAANNNNTTC 1 cut(s) 678
AspS9I GGNCC 3 cut(s) 637, 638, 925
AsuHPI GGTGA 3 cut(s) 484, 978, 984
AvaII GGWCC 1 cut(s) 925
BaeGI GKGCMC 2 cut(s) 546, 641
BalI TGGCCA 1 cut(s) 263
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 1142
BanII GRGCYC 2 cut(s) 641, 1153
BarI GAAGNNNNNNTAC 2 cut(s) 973, 1005
BbsI GAAGAC 2 cut(s) 639, 836
Bbv12I GWGCWC 2 cut(s) 546, 1153
BbvI GCAGC 2 cut(s) 1016, 1057
BccI CCATC 2 cut(s) 290, 415
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 2 cut(s) 572, 1320
BciVI GTATCC 2 cut(s) 425, 650
BcoDI GTCTC 4 cut(s) 363, 467, 582, 1105
BfaI CTAG 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 425, 650
BglII AGATCT 1 cut(s) 8
BisI GCNGC 2 cut(s) 1005, 1071
BlsI GCNGC 2 cut(s) 1006, 1072
BmcAI AGTACT 2 cut(s) 152, 1198
Bme1390I CCNGG 2 cut(s) 572, 1320
Bme18I GGWCC 1 cut(s) 925
BmgT120I GGNCC 3 cut(s) 637, 638, 925
BmiI GGNNCC 3 cut(s) 639, 762, 1144
BmrFI CCNGG 2 cut(s) 572, 1320
BmrI ACTGGG 1 cut(s) 1188
BmsI GCATC 4 cut(s) 568, 904, 923, 1023
BmuI ACTGGG 1 cut(s) 1188
BpiI GAAGAC 2 cut(s) 639, 836
BsaAI YACGTR 1 cut(s) 1278
BsaI GGTCTC 1 cut(s) 1105
BsaJI CCNNGG 3 cut(s) 764, 947, 1319
BsaWI WCCGGW 1 cut(s) 752
BsaXI ACNNNNNCTCC 2 cut(s) 1140, 1170
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 296, 1291
Bse1I ACTGG 1 cut(s) 1194
BseBI CCWGG 2 cut(s) 572, 1320
BseDI CCNNGG 3 cut(s) 764, 947, 1319
BseGI GGATG 2 cut(s) 361, 895
BseLI CCNNNNNNNGG 3 cut(s) 253, 296, 1291
BseMII CTCAG 2 cut(s) 59, 656
BseNI ACTGG 1 cut(s) 1194
BseRI GAGGAG 2 cut(s) 247, 406
BseSI GKGCMC 2 cut(s) 546, 641
BseXI GCAGC 2 cut(s) 1016, 1057
BshFI GGCC 4 cut(s) 93, 181, 263, 639
BshNI GGYRCC 1 cut(s) 1142
BsiHKAI GWGCWC 2 cut(s) 546, 1153
BsiSI CCGG 2 cut(s) 753, 1146
BsiWI CGTACG 1 cut(s) 1296
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 3 cut(s) 253, 296, 1291
BsmAI GTCTC 4 cut(s) 363, 467, 582, 1105
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 4 cut(s) 93, 181, 263, 639
Bso31I GGTCTC 1 cut(s) 1105
Bsp120I GGGCCC 1 cut(s) 637
Bsp1286I GDGCHC 3 cut(s) 546, 641, 1153
Bsp143I GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
Bsp19I CCATGG 1 cut(s) 764
BspACI CCGC 1 cut(s) 689
BspANI GGCC 4 cut(s) 93, 181, 263, 639
BspCNI CTCAG 2 cut(s) 58, 655
BspLI GGNNCC 3 cut(s) 639, 762, 1144
BspPI GGATC 5 cut(s) 412, 755, 768, 1192, 1259
BspT107I GGYRCC 1 cut(s) 1142
BspTNI GGTCTC 1 cut(s) 1105
BsrI ACTGG 1 cut(s) 1194
BssECI CCNNGG 3 cut(s) 764, 947, 1319
BssMI GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 572, 1320
Bst4CI ACNGT 2 cut(s) 465, 1301
Bst6I CTCTTC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 1278
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 7 cut(s) 28, 45, 458, 642, 801, 958, 1169
BstDSI CCRYGG 1 cut(s) 764
BstENI CCTNNNNNAGG 1 cut(s) 251
BstF5I GGATG 2 cut(s) 361, 895
BstKTI GATC 8 cut(s) 11, 51, 407, 763, 1021, 1042, 1187, 1267
BstMAI GTCTC 4 cut(s) 363, 467, 582, 1105
BstMBI GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
BstMWI GCNNNNNNNGC 2 cut(s) 99, 178
BstNI CCWGG 2 cut(s) 572, 1320
BstSCI CCNGG 2 cut(s) 570, 1318
BstSLI GKGCMC 2 cut(s) 546, 641
BstV1I GCAGC 2 cut(s) 1016, 1057
BstV2I GAAGAC 2 cut(s) 639, 836
BstX2I RGATCY 3 cut(s) 8, 404, 760
BstYI RGATCY 3 cut(s) 8, 404, 760
BsuI GTATCC 2 cut(s) 425, 650
BsuRI GGCC 4 cut(s) 93, 181, 263, 639
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 2 cut(s) 361, 895
Cac8I GCNNGC 1 cut(s) 600
Cfr13I GGNCC 3 cut(s) 637, 638, 925
Csp6I GTAC 5 cut(s) 151, 981, 1197, 1275, 1297
CviAII CATG 6 cut(s) 177, 259, 527, 765, 818, 1139
CviQI GTAC 5 cut(s) 151, 981, 1197, 1275, 1297
DdeI CTNAG 7 cut(s) 28, 45, 458, 642, 801, 958, 1169
DpnI GATC 8 cut(s) 10, 50, 406, 762, 1020, 1041, 1186, 1266
DpnII GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
EaeI YGGCCR 2 cut(s) 91, 261
Eam1104I CTCTTC 1 cut(s) 766
EarI CTCTTC 1 cut(s) 766
Ecl136II GAGCTC 1 cut(s) 1151
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 2 cut(s) 641, 1153
Eco31I GGTCTC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 925
Eco53kI GAGCTC 1 cut(s) 1151
Eco57I CTGAAG 2 cut(s) 875, 987
EcoICRI GAGCTC 1 cut(s) 1151
EcoNI CCTNNNNNAGG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 570, 1318
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 2 cut(s) 641, 1153
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 6 cut(s) 180, 262, 530, 768, 821, 1142
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 1 cut(s) 525
FatI CATG 6 cut(s) 176, 258, 526, 764, 817, 1138
FauI CCCGC 1 cut(s) 696
FauNDI CATATG 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 1005, 1071
FokI GGATG 2 cut(s) 348, 882
FriOI GRGCYC 2 cut(s) 641, 1153
Fsp4HI GCNGC 2 cut(s) 1005, 1071
FspBI CTAG 1 cut(s) 1037
GluI GCNGC 2 cut(s) 1005, 1071
HaeIII GGCC 4 cut(s) 93, 181, 263, 639
HapII CCGG 2 cut(s) 753, 1146
Hin1II CATG 6 cut(s) 180, 262, 530, 768, 821, 1142
HindIII AAGCTT 2 cut(s) 596, 955
HinfI GANTC 5 cut(s) 116, 343, 489, 863, 1229
HpaII CCGG 2 cut(s) 753, 1146
HphI GGTGA 3 cut(s) 484, 978, 984
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 7 cut(s) 8, 48, 442, 929, 948, 1044, 1228
Hpy188III TCNNGA 8 cut(s) 120, 290, 418, 654, 698, 1056, 1262, 1309
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 4 cut(s) 26, 445, 999, 1089
HpyCH4III ACNGT 2 cut(s) 465, 1301
HpyCH4IV ACGT 2 cut(s) 532, 1277
HpyCH4V TGCA 8 cut(s) 102, 508, 544, 732, 835, 914, 1108, 1130
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 178
HpyF3I CTNAG 7 cut(s) 28, 45, 458, 642, 801, 958, 1169
HpySE526I ACGT 2 cut(s) 532, 1277
Hsp92II CATG 6 cut(s) 180, 262, 530, 768, 821, 1142
Kzo9I GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
LmnI GCTCC 3 cut(s) 1067, 1148, 1156
Lsp1109I GCAGC 2 cut(s) 1016, 1057
LweI GCATC 4 cut(s) 568, 904, 923, 1023
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 2 cut(s) 532, 1277
MalI GATC 8 cut(s) 10, 50, 406, 762, 1020, 1041, 1186, 1266
MboI GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
MboII GAAGA 9 cut(s) 3, 135, 305, 341, 498, 573, 639, 783, 836
MfeI CAATTG 2 cut(s) 350, 918
MflI RGATCY 3 cut(s) 8, 404, 760
MhlI GDGCHC 3 cut(s) 546, 641, 1153
MlsI TGGCCA 1 cut(s) 263
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 1223
MmeI TCCRAC 3 cut(s) 952, 1162, 1251
Mox20I TGGCCA 1 cut(s) 263
MroXI GAANNNNTTC 1 cut(s) 678
MscI TGGCCA 1 cut(s) 263
MseI TTAA 7 cut(s) 138, 318, 555, 594, 905, 1163, 1212
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 1007
MspI CCGG 2 cut(s) 753, 1146
MspR9I CCNGG 2 cut(s) 572, 1320
MunI CAATTG 2 cut(s) 350, 918
MvaI CCWGG 2 cut(s) 572, 1320
MwoI GCNNNNNNNGC 2 cut(s) 99, 178
NcoI CCATGG 1 cut(s) 764
NdeI CATATG 1 cut(s) 202
NdeII GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
NlaIII CATG 6 cut(s) 180, 262, 530, 768, 821, 1142
NlaIV GGNNCC 3 cut(s) 639, 762, 1144
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 4 cut(s) 116, 343, 489, 863
Pfl23II CGTACG 1 cut(s) 1296
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 2 cut(s) 1006, 1072
PleI GAGTC 1 cut(s) 1223
PpsI GAGTC 1 cut(s) 1223
Ppu21I YACGTR 1 cut(s) 1278
Psp124BI GAGCTC 1 cut(s) 1153
Psp6I CCWGG 2 cut(s) 570, 1318
PspGI CCWGG 2 cut(s) 570, 1318
PspLI CGTACG 1 cut(s) 1296
PspN4I GGNNCC 3 cut(s) 639, 762, 1144
PspOMI GGGCCC 1 cut(s) 637
PspPI GGNCC 3 cut(s) 637, 638, 925
PsuI RGATCY 3 cut(s) 8, 404, 760
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 5 cut(s) 152, 982, 1198, 1276, 1298
RsaNI GTAC 5 cut(s) 151, 981, 1197, 1275, 1297
SacI GAGCTC 1 cut(s) 1153
SaqAI TTAA 7 cut(s) 138, 318, 555, 594, 905, 1163, 1212
SatI GCNGC 2 cut(s) 1005, 1071
Sau3AI GATC 8 cut(s) 8, 48, 404, 760, 1018, 1039, 1184, 1264
Sau96I GGNCC 3 cut(s) 637, 638, 925
ScaI AGTACT 2 cut(s) 152, 1198
SchI GAGTC 1 cut(s) 1223
ScrFI CCNGG 2 cut(s) 572, 1320
SduI GDGCHC 3 cut(s) 546, 641, 1153
SfaNI GCATC 4 cut(s) 568, 904, 923, 1023
SinI GGWCC 1 cut(s) 925
SsiI CCGC 1 cut(s) 689
SspI AATATT 5 cut(s) 370, 380, 521, 553, 1210
SspMI CTAG 1 cut(s) 1037
SstI GAGCTC 1 cut(s) 1153
StyD4I CCNGG 2 cut(s) 570, 1318
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 2 cut(s) 465, 1301
TaiI ACGT 2 cut(s) 535, 1280
TaqI TCGA 7 cut(s) 51, 449, 699, 785, 866, 1244, 1263
TatI WGTACW 2 cut(s) 150, 1196
TfiI GAWTC 4 cut(s) 116, 343, 489, 863
Tru1I TTAA 7 cut(s) 138, 318, 555, 594, 905, 1163, 1212
Tru9I TTAA 7 cut(s) 138, 318, 555, 594, 905, 1163, 1212
TseI GCWGC 2 cut(s) 1004, 1070
TspDTI ATGAA 6 cut(s) 189, 348, 543, 639, 806, 1244
TspGWI ACGGA 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 925
XagI CCTNNNNNAGG 1 cut(s) 251
XapI RAATTY 5 cut(s) 279, 285, 425, 806, 1331
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 1 cut(s) 1037
ZrmI AGTACT 2 cut(s) 152, 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.