Rh1DG238900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015688
Physical Location & Seq
Reverse (-)
45327546 .. 45332830
5285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG238900.1

Sequence Viewer

Length: 1044 bp
ATGGACTCGATTGGTGATGGGACGTCACAGACTCCGACTCAGTCTAGCATCGGAACTACACCAGCTTCGGCTGAAGCCGGACAACTCCTCATGTCTCCTTCTGTCGCTGCAACACCAACTTCTGCAGCAACCGGTGAAGCTCAAGAACTAGCGAAGAGGAAGAACCAGTCAGAAGGAAGAAATATCCTTGTTGATGCTTTTGGAGACATTAAGCTTGCTGATTTTGGTCTATCAGCACATTCAAACCTTTTGGGCGCTCAGTCTTCATCTGGACACAGACATGCTGTTGAAATCTTTCAACCCCCGCCTCATCTCGACCCATCTGCTGATATATGGTGTGTTGGGTGCATTATGATTGAGCTGACCACCGGAAAGGATCCATGGCGAAGAGAGAGCTGGTCGAATTATATTTTGCCTAAGAAATTTTCTTTTCATGCGAAAAACTTTTTGCAAGAGTGTCTTCAATGGGAACAACAAGATTCGAGGCTACCCACATTTGTCTTGCTTCAGCATCCATTCTTAAATCCTGCATTAATTGAGGTCCGACCTACTCAACTACTTCCGAGGGAAAGCTTAGTTGCTGAAGTGCTATATTGTACCTCACCTTCACCAACTTACGCAGCTGTTGGTGGAGATCATTCTAGATTGATTCTAGATCAGAATAACAGTTCAGGAGATGAGGGAGCTGCTGAACCTTCTCATAATGCTGAACCTCCTATAATGCAGAGACCTAATCAAGCCTTTGCACTTCAACATGGTGCCGGAGCTCCTGTCAGTTTAACTCTTGGTTCCAATGTTGGATCAACTCCCAGTACCAACTCGGAATCCATTTCCTCCCCTTTCGGTTCCGATGTCACTTTTGTGTCCGAACCTAGTTCTCATTCCCTTACTTCTTCCACCACATCCAGAAGTTCTTGTCCTAATTCTGAGTCCAATACTTCCGACTCCAAATCTATTTCGACACCTGTTCCTATTCTCAATCCAAACAGTACGTGTTTCCAAAAGGAGGCGTATAGATTCCAGGGGATAAAGAATTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

347

Amino Acids

37.16

Weight (kDa)

5.34

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 60 - 174 6.6e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 758
AciI CCGC 1 cut(s) 305
AclWI GGATC 3 cut(s) 371, 384, 808
AcsI RAATTY 2 cut(s) 422, 1033
AcuI CTGAAG 3 cut(s) 93, 491, 603
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 3 cut(s) 598, 814, 991
AfiI CCNNNNNNNGG 1 cut(s) 1006
AflIII ACRYGT 1 cut(s) 992
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 5 cut(s) 243, 290, 299, 464, 752
AjnI CCWGG 1 cut(s) 1020
AleI CACNNNNGTG 1 cut(s) 860
AluBI AGCT 9 cut(s) 65, 140, 214, 361, 396, 573, 623, 686, 767
AluI AGCT 9 cut(s) 65, 140, 214, 361, 396, 573, 623, 686, 767
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 3 cut(s) 99, 198, 721
AlwI GGATC 3 cut(s) 371, 384, 808
ApeKI GCWGC 4 cut(s) 107, 125, 620, 686
ApoI RAATTY 2 cut(s) 422, 1033
AseI ATTAAT 1 cut(s) 533
AsiGI ACCGGT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 541
AsuHPI GGTGA 4 cut(s) 26, 146, 594, 600
AvaII GGWCC 1 cut(s) 541
BamHI GGATCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 769
BarI GAAGNNNNNNTAC 2 cut(s) 589, 621
BbsI GAAGAC 2 cut(s) 255, 452
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 4 cut(s) 94, 137, 632, 673
BccI CCATC 2 cut(s) 11, 328
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 1022
BcoDI GTCTC 3 cut(s) 99, 198, 721
BfaI CTAG 5 cut(s) 45, 149, 642, 653, 873
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 4 cut(s) 108, 126, 621, 687
BlsI GCNGC 4 cut(s) 109, 127, 622, 688
Bme1390I CCNGG 1 cut(s) 1022
Bme18I GGWCC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 4 cut(s) 378, 760, 790, 847
BmrFI CCNGG 1 cut(s) 1022
BmrI ACTGGG 1 cut(s) 804
BmsI GCATC 3 cut(s) 57, 184, 520
BmuI ACTGGG 1 cut(s) 804
BpiI GAAGAC 2 cut(s) 255, 452
BpuEI CTTGAG 1 cut(s) 126
BsaAI YACGTR 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 3 cut(s) 380, 563, 1021
BsaWI WCCGGW 2 cut(s) 131, 368
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 1006
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 166, 810
BseBI CCWGG 1 cut(s) 1022
BseDI CCNNGG 3 cut(s) 380, 563, 1021
BseGI GGATG 2 cut(s) 511, 902
BseLI CCNNNNNNNGG 1 cut(s) 1006
BseMII CTCAG 3 cut(s) 53, 272, 918
BseNI ACTGG 2 cut(s) 166, 810
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 4 cut(s) 94, 137, 632, 673
BshNI GGYRCC 1 cut(s) 758
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 4 cut(s) 78, 132, 369, 762
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 1006
BsmAI GTCTC 3 cut(s) 99, 198, 721
BsmFI GGGAC 1 cut(s) 34
Bso31I GGTCTC 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 769
Bsp143I GATC 4 cut(s) 376, 634, 655, 800
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 1 cut(s) 305
BspCNI CTCAG 3 cut(s) 52, 271, 919
BspLI GGNNCC 4 cut(s) 378, 760, 790, 847
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 3 cut(s) 371, 384, 808
BspT107I GGYRCC 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 721
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 166, 810
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 380, 563, 1021
BssMI GATC 4 cut(s) 376, 634, 655, 800
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 1022
Bst4CI ACNGT 2 cut(s) 668, 989
Bst6I CTCTTC 2 cut(s) 149, 382
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 993
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 5 cut(s) 39, 258, 417, 574, 927
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 2 cut(s) 511, 902
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 4 cut(s) 379, 637, 658, 803
BstMAI GTCTC 3 cut(s) 99, 198, 721
BstMBI GATC 4 cut(s) 376, 634, 655, 800
BstNI CCWGG 1 cut(s) 1022
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 1020
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 4 cut(s) 94, 137, 632, 673
BstV2I GAAGAC 2 cut(s) 255, 452
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 2 cut(s) 511, 902
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 541
Csp6I GTAC 3 cut(s) 597, 813, 990
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 5 cut(s) 91, 281, 381, 434, 755
CviQI GTAC 3 cut(s) 597, 813, 990
DdeI CTNAG 5 cut(s) 39, 258, 417, 574, 927
DpnI GATC 4 cut(s) 378, 636, 657, 802
DpnII GATC 4 cut(s) 376, 634, 655, 800
Eam1104I CTCTTC 2 cut(s) 149, 382
EarI CTCTTC 2 cut(s) 149, 382
Ecl136II GAGCTC 1 cut(s) 767
Eco130I CCWWGG 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 769
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 767
Eco57I CTGAAG 3 cut(s) 93, 491, 603
EcoICRI GAGCTC 1 cut(s) 767
EcoRII CCWGG 1 cut(s) 1020
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 5 cut(s) 94, 284, 384, 437, 758
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 1 cut(s) 34
FatI CATG 5 cut(s) 90, 280, 380, 433, 754
FauI CCCGC 1 cut(s) 312
Fnu4HI GCNGC 4 cut(s) 108, 126, 621, 687
FokI GGATG 2 cut(s) 498, 889
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 4 cut(s) 108, 126, 621, 687
FspBI CTAG 5 cut(s) 45, 149, 642, 653, 873
GlaI GCGC 1 cut(s) 256
GluI GCNGC 4 cut(s) 108, 126, 621, 687
HaeII RGCGCY 1 cut(s) 258
HapII CCGG 4 cut(s) 78, 132, 369, 762
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 5 cut(s) 94, 284, 384, 437, 758
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HindIII AAGCTT 2 cut(s) 212, 571
HinfI GANTC 9 cut(s) 5, 31, 37, 479, 649, 824, 929, 944, 1017
HpaII CCGG 4 cut(s) 78, 132, 369, 762
HphI GGTGA 4 cut(s) 26, 146, 594, 600
Hpy188III TCNNGA 7 cut(s) 143, 270, 314, 642, 653, 672, 906
HpyAV CCTTC 4 cut(s) 108, 167, 615, 705
HpyCH4III ACNGT 2 cut(s) 668, 989
HpyCH4IV ACGT 2 cut(s) 23, 992
HpyCH4V TGCA 7 cut(s) 110, 125, 348, 451, 530, 724, 746
HpyF3I CTNAG 5 cut(s) 39, 258, 417, 574, 927
HpySE526I ACGT 2 cut(s) 23, 992
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 5 cut(s) 94, 284, 384, 437, 758
HspAI GCGC 1 cut(s) 255
Kzo9I GATC 4 cut(s) 376, 634, 655, 800
LmnI GCTCC 3 cut(s) 683, 764, 772
Lsp1109I GCAGC 4 cut(s) 94, 137, 632, 673
LweI GCATC 3 cut(s) 57, 184, 520
MaeI CTAG 5 cut(s) 45, 149, 642, 653, 873
MaeII ACGT 2 cut(s) 23, 992
MaeIII GTNAC 2 cut(s) 24, 853
MalI GATC 4 cut(s) 378, 636, 657, 802
MboI GATC 4 cut(s) 376, 634, 655, 800
MboII GAAGA 7 cut(s) 166, 172, 189, 255, 399, 452, 885
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 769
MluCI AATT 5 cut(s) 403, 422, 534, 922, 1033
MlyI GAGTC 4 cut(s) 25, 31, 938, 938
MmeI TCCRAC 4 cut(s) 59, 568, 778, 966
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 4 cut(s) 210, 521, 533, 779
MslI CAYNNNNRTG 2 cut(s) 279, 860
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 78, 132, 369, 762
MspR9I CCNGG 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 1022
NcoI CCATGG 1 cut(s) 380
NdeII GATC 4 cut(s) 376, 634, 655, 800
NlaIII CATG 5 cut(s) 94, 284, 384, 437, 758
NlaIV GGNNCC 4 cut(s) 378, 760, 790, 847
NmuCI GTSAC 2 cut(s) 24, 853
NspI RCATGY 1 cut(s) 284
OliI CACNNNNGTG 1 cut(s) 860
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 4 cut(s) 479, 649, 824, 1017
PflFI GACNNNGTC 1 cut(s) 40
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 4 cut(s) 109, 127, 622, 688
PleI GAGTC 4 cut(s) 25, 31, 937, 938
PpsI GAGTC 4 cut(s) 25, 31, 937, 938
Ppu21I YACGTR 1 cut(s) 993
PshBI ATTAAT 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 769
Psp6I CCWGG 1 cut(s) 1020
PspGI CCWGG 1 cut(s) 1020
PspN4I GGNNCC 4 cut(s) 378, 760, 790, 847
PspPI GGNCC 1 cut(s) 541
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 3 cut(s) 598, 814, 991
RsaNI GTAC 3 cut(s) 597, 813, 990
RseI CAYNNNNRTG 2 cut(s) 279, 860
SacI GAGCTC 1 cut(s) 769
SaqAI TTAA 4 cut(s) 210, 521, 533, 779
SatI GCNGC 4 cut(s) 108, 126, 621, 687
Sau3AI GATC 4 cut(s) 376, 634, 655, 800
Sau96I GGNCC 1 cut(s) 541
SchI GAGTC 4 cut(s) 25, 31, 938, 938
ScrFI CCNGG 1 cut(s) 1022
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 3 cut(s) 57, 184, 520
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 279, 860
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 5 cut(s) 403, 422, 534, 922, 1033
SsiI CCGC 1 cut(s) 305
SspMI CTAG 5 cut(s) 45, 149, 642, 653, 873
SstI GAGCTC 1 cut(s) 769
StyD4I CCNGG 1 cut(s) 1020
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 2 cut(s) 668, 989
TaiI ACGT 2 cut(s) 26, 995
TaqI TCGA 5 cut(s) 8, 315, 401, 482, 959
TasI AATT 5 cut(s) 403, 422, 534, 922, 1033
TfiI GAWTC 4 cut(s) 479, 649, 824, 1017
Tru1I TTAA 4 cut(s) 210, 521, 533, 779
Tru9I TTAA 4 cut(s) 210, 521, 533, 779
TseFI GTSAC 2 cut(s) 24, 853
TseI GCWGC 4 cut(s) 107, 125, 620, 686
Tsp45I GTSAC 2 cut(s) 24, 853
TspDTI ATGAA 2 cut(s) 255, 422
Tth111I GACNNNGTC 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 541
VspI ATTAAT 1 cut(s) 533
XapI RAATTY 2 cut(s) 422, 1033
XbaI TCTAGA 2 cut(s) 641, 652
XceI RCATGY 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 5 cut(s) 45, 149, 642, 653, 873
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.