Rh1BG212700

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0015688
Physical Location & Seq
Reverse (-)
33163572 .. 33169606
6035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG212700.1

Sequence Viewer

Length: 951 bp
ATGGAATTCTGGGGCAAATCAGACTGCTCGAAGGAACTAAGACTGTTCAACGAGACAGGAAGAAATATCCTTGTTGATGCTTTTGGAGACATTAAGCTTGCTGATTTTGGTCTATCAGCACATTCAAACCTTTTGGGCGCTCAGTCTTCATCTGGACACAGACATGCTGTTGAAATCTTTCAACCCCCGCCTCATCTCGACCCATCTGCTGATATATGGTGTGTTGGGTGCATTATGATTGAGCTGACCACCGGAAAGGATCCATGGCGAAGAGAGAGCTGGTCGAATTATATTTTGCCTAAGAAATTTTCTTTTCATGCGAAAAACTTTTTGCAAGAGTGTCTTCAATGGGAACAACAAGATTCGAGGCTACCCACATTTGTCTTGCTTCAGCATCCATTCTTAAATCCTGCATTAATTGAGGTCCGACCTACTCAACTACTTCCGAGGGAAAGCTTAGTTGCTGAAGTGCTATATTGTACCTCACCTTCACCAACTTACGCAGCTGTTGGTGGAGATCATTCTAGATTGATTCTAGATCAGAATAACAGTTCAGGAGATGAGGGAGCTGCTGAACCTTCTCATAATGCTGAACCTCCTATAATGCAGAGACCTAATCAAGCCTTTGCACTTCAACATGGTGCCGGAGCTCCTGTCAGTTTAACTCTTGGTTCCAATGTTGGATCAACTCCCAGTACCAACTCGGAATCCATTTCCTCCCCTTTCGGTTCCGATGTCACTTTTGTGTCCGAACCTAGTTCTCATTCCCTTACTTCTTCCACCACATCCAGAAGTTCTTGTCCTAATTCTGAGTCCAATACTTCCGACTCCAAATCTATTTCGACACCTGTTCCTATTCTCAATCCAAACAGTACGTGTTTCCAAAAGGAGGCGTATGTACGTACACGTCGATCCGGGTTCCTTAAGTTCTTGTGTTTTTATTCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

316

Amino Acids

34.62

Weight (kDa)

5.79

Isoelectric Point (pI)

64.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 135 5.4e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AciI CCGC 1 cut(s) 188
AclWI GGATC 4 cut(s) 254, 267, 691, 906
AcsI RAATTY 2 cut(s) 5, 305
AcuI CTGAAG 2 cut(s) 374, 486
AfaI GTAC 5 cut(s) 481, 697, 874, 900, 904
AfiI CCNNNNNNNGG 1 cut(s) 889
AflII CTTAAG 1 cut(s) 923
AflIII ACRYGT 2 cut(s) 875, 905
AgsI TTSAA 6 cut(s) 49, 126, 173, 182, 347, 635
AjiI CACGTC 1 cut(s) 908
AleI CACNNNNGTG 1 cut(s) 743
AluBI AGCT 7 cut(s) 97, 244, 279, 456, 506, 569, 650
AluI AGCT 7 cut(s) 97, 244, 279, 456, 506, 569, 650
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 3 cut(s) 47, 81, 604
AlwI GGATC 4 cut(s) 254, 267, 691, 906
ApeKI GCWGC 2 cut(s) 503, 569
ApoI RAATTY 2 cut(s) 5, 305
AseI ATTAAT 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 916
AsuHPI GGTGA 2 cut(s) 477, 483
AvaII GGWCC 1 cut(s) 424
BamHI GGATCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 652
BarI GAAGNNNNNNTAC 2 cut(s) 472, 504
BbsI GAAGAC 2 cut(s) 138, 335
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 2 cut(s) 515, 556
BccI CCATC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BcnI CCSGG 1 cut(s) 916
BcoDI GTCTC 3 cut(s) 47, 81, 604
BfaI CTAG 3 cut(s) 525, 536, 756
BfoI RGCGCY 1 cut(s) 141
BfrI CTTAAG 1 cut(s) 923
BisI GCNGC 2 cut(s) 504, 570
BlsI GCNGC 2 cut(s) 505, 571
Bme1390I CCNGG 1 cut(s) 916
Bme18I GGWCC 1 cut(s) 424
BmgBI CACGTC 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 5 cut(s) 261, 643, 673, 730, 920
BmrFI CCNGG 1 cut(s) 916
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 2 cut(s) 67, 403
BmuI ACTGGG 1 cut(s) 687
BpiI GAAGAC 2 cut(s) 138, 335
BpuMI CCSGG 1 cut(s) 916
BsaAI YACGTR 2 cut(s) 876, 902
BsaI GGTCTC 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 263, 446
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse1I ACTGG 1 cut(s) 693
BseDI CCNNGG 2 cut(s) 263, 446
BseGI GGATG 2 cut(s) 394, 785
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMII CTCAG 2 cut(s) 155, 801
BseNI ACTGG 1 cut(s) 693
BseXI GCAGC 2 cut(s) 515, 556
BshNI GGYRCC 1 cut(s) 641
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 3 cut(s) 252, 645, 915
BslI CCNNNNNNNGG 1 cut(s) 889
BsmAI GTCTC 3 cut(s) 47, 81, 604
Bso31I GGTCTC 1 cut(s) 604
Bsp1286I GDGCHC 1 cut(s) 652
Bsp143I GATC 5 cut(s) 259, 517, 538, 683, 911
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 154, 802
BspLI GGNNCC 5 cut(s) 261, 643, 673, 730, 920
BspPI GGATC 4 cut(s) 254, 267, 691, 906
BspT107I GGYRCC 1 cut(s) 641
BspTI CTTAAG 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 604
BsrI ACTGG 1 cut(s) 693
BssECI CCNNGG 2 cut(s) 263, 446
BssMI GATC 5 cut(s) 259, 517, 538, 683, 911
BssT1I CCWWGG 1 cut(s) 263
Bst4CI ACNGT 3 cut(s) 45, 551, 872
Bst6I CTCTTC 1 cut(s) 265
BstAFI CTTAAG 1 cut(s) 923
BstBAI YACGTR 2 cut(s) 876, 902
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 5 cut(s) 38, 141, 300, 457, 810
BstDSI CCRYGG 1 cut(s) 263
BstF5I GGATG 2 cut(s) 394, 785
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 5 cut(s) 262, 520, 541, 686, 914
BstMAI GTCTC 3 cut(s) 47, 81, 604
BstMBI GATC 5 cut(s) 259, 517, 538, 683, 911
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 914
BstSNI TACGTA 1 cut(s) 902
BstV1I GCAGC 2 cut(s) 515, 556
BstV2I GAAGAC 2 cut(s) 138, 335
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 908
BtsCI GGATG 2 cut(s) 394, 785
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 5 cut(s) 480, 696, 873, 899, 903
CviAII CATG 4 cut(s) 164, 264, 317, 638
CviJI RGCY 9 cut(s) 97, 244, 279, 370, 456, 506, 569, 623, 650
CviKI_1 RGCY 9 cut(s) 97, 244, 279, 370, 456, 506, 569, 623, 650
CviQI GTAC 5 cut(s) 480, 696, 873, 899, 903
DdeI CTNAG 5 cut(s) 38, 141, 300, 457, 810
DpnI GATC 5 cut(s) 261, 519, 540, 685, 913
DpnII GATC 5 cut(s) 259, 517, 538, 683, 911
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Ecl136II GAGCTC 1 cut(s) 650
Eco105I TACGTA 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 604
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 650
Eco57I CTGAAG 2 cut(s) 374, 486
EcoICRI GAGCTC 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 652
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 4 cut(s) 167, 267, 320, 641
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FatI CATG 4 cut(s) 163, 263, 316, 637
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 504, 570
FokI GGATG 2 cut(s) 381, 772
FriOI GRGCYC 1 cut(s) 652
Fsp4HI GCNGC 2 cut(s) 504, 570
FspBI CTAG 3 cut(s) 525, 536, 756
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 504, 570
HaeII RGCGCY 1 cut(s) 141
HapII CCGG 3 cut(s) 252, 645, 915
HhaI GCGC 1 cut(s) 140
Hin1II CATG 4 cut(s) 167, 267, 320, 641
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 2 cut(s) 95, 454
HinfI GANTC 5 cut(s) 362, 532, 707, 812, 827
HpaII CCGG 3 cut(s) 252, 645, 915
HphI GGTGA 2 cut(s) 477, 483
Hpy166II GTNNAC 1 cut(s) 905
Hpy188I TCNGA 9 cut(s) 22, 428, 447, 543, 706, 733, 751, 811, 826
Hpy188III TCNNGA 6 cut(s) 153, 197, 525, 536, 555, 789
Hpy8I GTNNAC 1 cut(s) 905
Hpy99I CGWCG 1 cut(s) 912
HpyAV CCTTC 3 cut(s) 25, 498, 588
HpyCH4III ACNGT 3 cut(s) 45, 551, 872
HpyCH4IV ACGT 3 cut(s) 875, 901, 907
HpyCH4V TGCA 5 cut(s) 231, 334, 413, 607, 629
HpyF3I CTNAG 5 cut(s) 38, 141, 300, 457, 810
HpySE526I ACGT 3 cut(s) 875, 901, 907
Hsp92II CATG 4 cut(s) 167, 267, 320, 641
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 5 cut(s) 259, 517, 538, 683, 911
LmnI GCTCC 3 cut(s) 566, 647, 655
Lsp1109I GCAGC 2 cut(s) 515, 556
LweI GCATC 2 cut(s) 67, 403
MaeI CTAG 3 cut(s) 525, 536, 756
MaeII ACGT 3 cut(s) 875, 901, 907
MaeIII GTNAC 1 cut(s) 736
MalI GATC 5 cut(s) 261, 519, 540, 685, 913
MboI GATC 5 cut(s) 259, 517, 538, 683, 911
MboII GAAGA 5 cut(s) 72, 138, 282, 335, 768
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 652
MluCI AATT 5 cut(s) 5, 286, 305, 417, 805
MlyI GAGTC 2 cut(s) 821, 821
MmeI TCCRAC 3 cut(s) 451, 661, 849
MnlI CCTC 9 cut(s) 201, 360, 415, 441, 493, 556, 606, 727, 883
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 5 cut(s) 93, 404, 416, 662, 924
MslI CAYNNNNRTG 2 cut(s) 162, 743
MspA1I CMGCKG 1 cut(s) 506
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 3 cut(s) 252, 645, 915
MspR9I CCNGG 1 cut(s) 916
NciI CCSGG 1 cut(s) 916
NcoI CCATGG 1 cut(s) 263
NdeII GATC 5 cut(s) 259, 517, 538, 683, 911
NlaIII CATG 4 cut(s) 167, 267, 320, 641
NlaIV GGNNCC 5 cut(s) 261, 643, 673, 730, 920
NmuCI GTSAC 1 cut(s) 736
NspI RCATGY 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 743
PcsI WCGNNNNNNNCGW 1 cut(s) 907
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 3 cut(s) 362, 532, 707
PkrI GCNGC 2 cut(s) 505, 571
PleI GAGTC 2 cut(s) 820, 821
PpsI GAGTC 2 cut(s) 820, 821
Ppu21I YACGTR 2 cut(s) 876, 902
PshBI ATTAAT 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 652
PspN4I GGNNCC 5 cut(s) 261, 643, 673, 730, 920
PspPI GGNCC 1 cut(s) 424
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 5 cut(s) 481, 697, 874, 900, 904
RsaNI GTAC 5 cut(s) 480, 696, 873, 899, 903
RseI CAYNNNNRTG 2 cut(s) 162, 743
SacI GAGCTC 1 cut(s) 652
SaqAI TTAA 5 cut(s) 93, 404, 416, 662, 924
SatI GCNGC 2 cut(s) 504, 570
Sau3AI GATC 5 cut(s) 259, 517, 538, 683, 911
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 2 cut(s) 821, 821
ScrFI CCNGG 1 cut(s) 916
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 2 cut(s) 67, 403
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 2 cut(s) 162, 743
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
SnaBI TACGTA 1 cut(s) 902
Sse9I AATT 5 cut(s) 5, 286, 305, 417, 805
SsiI CCGC 1 cut(s) 188
SspMI CTAG 3 cut(s) 525, 536, 756
SstI GAGCTC 1 cut(s) 652
StyD4I CCNGG 1 cut(s) 914
StyI CCWWGG 1 cut(s) 263
TaaI ACNGT 3 cut(s) 45, 551, 872
TaiI ACGT 3 cut(s) 878, 904, 910
TaqI TCGA 6 cut(s) 29, 198, 284, 365, 842, 910
TasI AATT 5 cut(s) 5, 286, 305, 417, 805
TfiI GAWTC 3 cut(s) 362, 532, 707
Tru1I TTAA 5 cut(s) 93, 404, 416, 662, 924
Tru9I TTAA 5 cut(s) 93, 404, 416, 662, 924
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 2 cut(s) 503, 569
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 2 cut(s) 138, 305
Vha464I CTTAAG 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 424
VspI ATTAAT 1 cut(s) 416
XapI RAATTY 2 cut(s) 5, 305
XbaI TCTAGA 2 cut(s) 524, 535
XceI RCATGY 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 3 cut(s) 525, 536, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.