Rorug01G0228300

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015688
Physical Location & Seq
Forward (+)
32916124 .. 32917346
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0228300.1

Sequence Viewer

Length: 552 bp
ATGGCCCTGGCAATAGCTTTCCTTATACTCATGGTGGCAGCTCCAGCTGCAGTTTATGGAGCTGAGCAGACTGTTGGTGATACCCAGGGATGGAACCAAGGTGTAAATTATGACACTTGGGCTTCCGGCAAAACCTTCACCGTCGGTGACACTCTTGTGTTCAACTATGACAGTAGCCACAAAGTTGATGAGGTAAACCTAGCAGACTACAACGGTTGCAGTTCAAGTAATTCCATCAAAACCTATCAGGATAGTCCCACAAAAATCACCCTCTCCAAAGCTGGTCCAAGCTACTACATATGCCCTATCTCAGGTCACTGTGCTAGTGGCATGAAGCTTTTGGTCAATGTTGTGGCGGCTGGCACCCCAAGTGGCACCCCGCCTACTACTACCCCCGCCACGCCACCTCCACCCACCACCACAACTCCTGCCAATAATGACTCGCCACCTCCGCCACCGCCACCGCCTAAGATATCACCAGCGAGTAGTCTTCACATGAATTTGGTTGGGGTTCCACTTGCGTTGGCAACCTTGATGGCAGTTATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

183

Amino Acids

18.82

Weight (kDa)

5.36

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 31 - 111 2.6e-27 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 362, 374
AciI CCGC 6 cut(s) 356, 380, 396, 452, 458, 464
AcsI RAATTY 1 cut(s) 499
AfiI CCNNNNNNNGG 2 cut(s) 90, 311
AgsI TTSAA 2 cut(s) 163, 225
AjnI CCWGG 2 cut(s) 6, 84
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 7 cut(s) 17, 41, 47, 62, 281, 291, 337
AluI AGCT 7 cut(s) 17, 41, 47, 62, 281, 291, 337
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 38, 47
ApoI RAATTY 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 4, 284
AsuHPI GGTGA 5 cut(s) 89, 130, 158, 259, 468
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 2 cut(s) 362, 374
BbsI GAAGAC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 34, 50
BccI CCATC 3 cut(s) 84, 242, 529
BciT130I CCWGG 2 cut(s) 8, 86
BfaI CTAG 3 cut(s) 200, 324, 550
BfmI CTRYAG 1 cut(s) 48
BisI GCNGC 3 cut(s) 39, 48, 357
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 3 cut(s) 40, 49, 358
Bme1390I CCNGG 2 cut(s) 8, 86
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 4, 284
BmiI GGNNCC 4 cut(s) 95, 364, 376, 513
BmrFI CCNGG 2 cut(s) 8, 86
BpiI GAAGAC 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 6, 84, 85, 97
BsaXI ACNNNNNCTCC 4 cut(s) 391, 409, 421, 439
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 311
BseBI CCWGG 2 cut(s) 8, 86
BseDI CCNNGG 4 cut(s) 6, 84, 85, 97
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 90, 311
BseMII CTCAG 2 cut(s) 54, 324
BseXI GCAGC 2 cut(s) 34, 50
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 362, 374
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 240
BslI CCNNNNNNNGG 2 cut(s) 90, 311
BsmFI GGGAC 1 cut(s) 240
BsnI GGCC 1 cut(s) 5
Bsp1720I GCTNAGC 1 cut(s) 63
BspACI CCGC 6 cut(s) 356, 380, 396, 452, 458, 464
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 55, 323
BspLI GGNNCC 4 cut(s) 95, 364, 376, 513
BspMAI CTGCAG 1 cut(s) 52
BspT107I GGYRCC 2 cut(s) 362, 374
BssECI CCNNGG 4 cut(s) 6, 84, 85, 97
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 8, 86
Bst4CI ACNGT 5 cut(s) 73, 142, 173, 215, 320
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 3 cut(s) 63, 310, 468
BstENI CCTNNNNNAGG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 95
BstMWI GCNNNNNNNGC 3 cut(s) 44, 47, 451
BstNI CCWGG 2 cut(s) 8, 86
BstSCI CCNGG 2 cut(s) 6, 84
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 34, 50
BstV2I GAAGAC 1 cut(s) 482
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 361
Cfr13I GGNCC 2 cut(s) 4, 284
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviAII CATG 3 cut(s) 31, 331, 496
DdeI CTNAG 3 cut(s) 63, 310, 468
EciI GGCGGA 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 97
Eco32I GATATC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 6, 84
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 34, 334, 499
FaqI GGGAC 1 cut(s) 240
FatI CATG 3 cut(s) 30, 330, 495
FauI CCCGC 2 cut(s) 387, 403
FauNDI CATATG 1 cut(s) 299
Fnu4HI GCNGC 3 cut(s) 39, 48, 357
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 39, 48, 357
FspBI CTAG 3 cut(s) 200, 324, 550
GluI GCNGC 3 cut(s) 39, 48, 357
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 126
Hin1II CATG 3 cut(s) 34, 334, 499
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 1 cut(s) 440
HpaII CCGG 1 cut(s) 126
HphI GGTGA 5 cut(s) 89, 130, 158, 259, 468
Hpy166II GTNNAC 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 5 cut(s) 73, 142, 173, 215, 320
HpyCH4V TGCA 2 cut(s) 50, 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 47, 451
HpyF3I CTNAG 3 cut(s) 63, 310, 468
Hsp92II CATG 3 cut(s) 34, 334, 499
LmnI GCTCC 2 cut(s) 46, 59
Lsp1109I GCAGC 2 cut(s) 34, 50
MaeI CTAG 3 cut(s) 200, 324, 550
MaeIII GTNAC 2 cut(s) 146, 314
MboII GAAGA 1 cut(s) 482
MluCI AATT 3 cut(s) 106, 229, 499
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 4 cut(s) 184, 281, 417, 459
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 2 cut(s) 8, 86
MvaI CCWGG 2 cut(s) 8, 86
MwoI GCNNNNNNNGC 3 cut(s) 44, 47, 451
NdeI CATATG 1 cut(s) 299
NlaIII CATG 3 cut(s) 34, 334, 499
NlaIV GGNNCC 4 cut(s) 95, 364, 376, 513
NmuCI GTSAC 2 cut(s) 146, 314
OliI CACNNNNGTG 1 cut(s) 155
PasI CCCWGGG 1 cut(s) 85
PkrI GCNGC 3 cut(s) 40, 49, 358
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 6, 84
PspGI CCWGG 2 cut(s) 6, 84
PspN4I GGNNCC 4 cut(s) 95, 364, 376, 513
PspPI GGNCC 2 cut(s) 4, 284
PstI CTGCAG 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 155
SatI GCNGC 3 cut(s) 39, 48, 357
Sau96I GGNCC 2 cut(s) 4, 284
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 2 cut(s) 8, 86
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 3 cut(s) 106, 229, 499
SsiI CCGC 6 cut(s) 356, 380, 396, 452, 458, 464
SspMI CTAG 3 cut(s) 200, 324, 550
StyD4I CCNGG 2 cut(s) 6, 84
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 5 cut(s) 73, 142, 173, 215, 320
TasI AATT 3 cut(s) 106, 229, 499
TauI GCSGC 1 cut(s) 359
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 2 cut(s) 146, 314
TseI GCWGC 2 cut(s) 38, 47
Tsp45I GTSAC 2 cut(s) 146, 314
TspDTI ATGAA 2 cut(s) 347, 512
TspRI CASTG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 284
XagI CCTNNNNNAGG 1 cut(s) 309
XapI RAATTY 1 cut(s) 499
XspI CTAG 3 cut(s) 200, 324, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.