Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0007964

Physical Location & Seq

Genomic Coordinates Reverse (-)

25910726 .. 25915493

Gene Structure 4768 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00305270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGTCCTCGCTTCCCATTTCTTCTAAATCAAATTCCTCCAACTTCTCCGCATCAAAGAATCCTACTTTTCTAGACCCAAAAACTCGAACTCCGTATCCCCAACATGATCTTATTGTTCTGGGCATCGAAACCAGCTGTGACGACACCGCCGCCGCCGTCGTGAAAAGCAACGGTGAAATTCTCAGCCAAGTTATTTCTTCTCAGGCAGATTTGCTTGCTCAATATGGGGGTGTTGCTCCCAAAATGGCAGAAGAAGCGCACTTGCAAGTGATTGATCGGGTTGTGCAAGAAGCGCTTGACAAAGCTAATTTAACCGAGAGAGATTTGTCTGCAGTTGCTGTTACCATTGGTCCGGGTTTAAGCCTTTGTCTTCGTGTTGGTGTGCGGAAAGCTCGTAAAATTGCTGGTACTTTTAGCTTACCAATTATTGGTGTACATCACATGGAGGCTCATGCTCTTGTTGCCAGGTTAACTGAACAAGAACTACAATTTCCTTTTATGGCTCTGCTTATTTCAGGAGGACACAATGTACTCATCCTTGCTCGTGATCTTGGTCATTATGTACAACTTGGGACCACAATAGATGATGCTATTGGTGAGGCATATGATAAGATAGCAAAATGGCTTGGTCTTGACTTGAGGAGAAGTGGTGGGCCAGCTGTAGAAGAGCTTGCCCGAGAGGGTGATGCAAAATCAATCAAATTCTCGACTCCAATGAAACAACATAAAGATTGCAACTTCTCGTACGCTGGTCTGAAGACTCAAGTGAGGCTGGCAATTGAATCTAAAAATATTAATGCTACAATTCCTGTTTCTTCAGCAACTAGCCAAGATAGAAGGTCCCGAGCTGATATTGCTGCCTCTTTTCAGAGAGTTGCAGTATTACATCTGGAAGAAAGGTGTGAACGAGCAATTGAATGGGCTTTGAAGATTGAACCTTCTGTAAAACATATGGTTGTGTCTGGTGGTGTTGCATCAAATCAATATGTTCGGGCTCAACTTGATCAGGTTGTCAAGAAAAACAACTTACAACTTGTATGCCCTCCTCCCAGCCTTTGTACAGACAACGGTGTAATGGTGGCATGGACTGGCATTGAGAACTTTTGTATGGGAAGATTTGATCCACCACCACCTGCAGATGAACCTGAAGATATTGAGTTTGATCTGCGGCCAAGGTGGCCATTGGGGGAAGAATACGCTGAGGGAAGGAGTGAAGCTCGGTCAATGAGAACAGCCCGCATTCATCCGTCTCTGACATCTCTCGTCCAAGCATCTTTGCAACAGCAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000408 GO:0002949 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016020 GO:0016070 GO:0019866 GO:0022414 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070525 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M22

Protein Analysis

430

Amino Acids

46.93

Weight (kDa)

6.28

Isoelectric Point (pI)

54.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TsaD	PF00814	59 - 362	2.9e-85	tRNA N6-adenosine threonylcarbamoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45270
fragaria_vesca	FvH4_7g11490 FvH4_7g11490 FvH4_7g11490
malus_domestica	MD02G1206900.v1.1
prunus_persica	Prupe.2G143300_v2.0.a1
pyrus_communis	pycom02g16910
rosa_chinensis	RchiOBHm_Chr1g0349791 RchiOBHm_Chr1g0349801 RchiOBHm_Chr1g0349851 RchiOBHm_Chr1g0349931
rosa_laevigata	RLG00000028515
rosa_multiflora	Rmu_sc0000778.1_g000003 Rmu_sc0005220.1_g000015 Rmu_sc0007377.1_g000005
rosa_roxburghii	Rroxscaffold_4G00305270
rosa_rugosa	Rorug01G0207000 Rorug01G0207000
rosa_samantha	Rh1AG223600 Rh1BG190800 Rh1CG208400
rosa_wichuraiana	Rw0G007620 Rw1G019050 Rw1G019060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1141
Acc36I	ACCTGC	1 cut(s)	1141
AccB7I	CCANNNNNTGG	1 cut(s)	428
AciI	CCGC	7 cut(s)	48, 147, 150, 153, 385, 1168, 1237
AclWI	GGATC	1 cut(s)	1115
AcoI	YGGCCR	2 cut(s)	1169, 1178
AcsI	RAATTY	3 cut(s)	31, 177, 701
AcuI	CTGAAG	3 cut(s)	776, 801, 1167
AfaI	GTAC	6 cut(s)	409, 435, 531, 564, 746, 1060
AfeI	AGCGCT	1 cut(s)	294
AfiI	CCNNNNNNNGG	1 cut(s)	428
AgsI	TTSAA	4 cut(s)	782, 917, 928, 935
AjnI	CCWGG	1 cut(s)	464
AloI	GAACNNNNNNTCC	2 cut(s)	79, 111
AluBI	AGCT	8 cut(s)	135, 305, 392, 417, 659, 670, 848, 1217
AluI	AGCT	8 cut(s)	135, 305, 392, 417, 659, 670, 848, 1217
Alw26I	GTCTC	1 cut(s)	1254
AlwI	GGATC	1 cut(s)	1115
AlwNI	CAGNNNCTG	1 cut(s)	338
Ama87I	CYCGRG	2 cut(s)	675, 843
Aor51HI	AGCGCT	1 cut(s)	294
AoxI	GGCC	3 cut(s)	653, 1169, 1178
ApeKI	GCWGC	1 cut(s)	857
ApoI	RAATTY	3 cut(s)	31, 177, 701
AseI	ATTAAT	1 cut(s)	795
AspLEI	GCGC	2 cut(s)	259, 295
AspS9I	GGNCC	4 cut(s)	350, 573, 653, 840
AsuC2I	CCSGG	1 cut(s)	354
AsuHPI	GGTGA	3 cut(s)	185, 608, 695
AvaI	CYCGRG	2 cut(s)	675, 843
AvaII	GGWCC	3 cut(s)	350, 573, 840
BalI	TGGCCA	1 cut(s)	1180
BanII	GRGCYC	1 cut(s)	997
BauI	CACGAG	1 cut(s)	543
BbsI	GAAGAC	2 cut(s)	362, 764
BbvCI	CCTCAGC	1 cut(s)	1200
BbvI	GCAGC	1 cut(s)	844
BceAI	ACGGC	1 cut(s)	140
BciT130I	CCWGG	1 cut(s)	466
BciVI	GTATCC	1 cut(s)	105
BclI	TGATCA	1 cut(s)	1003
BcnI	CCSGG	1 cut(s)	354
BcoDI	GTCTC	1 cut(s)	1254
BfaI	CTAG	2 cut(s)	71, 825
BfmI	CTRYAG	4 cut(s)	330, 660, 1134, 1289
BfoI	RGCGCY	1 cut(s)	296
BfuAI	ACCTGC	1 cut(s)	1141
BfuI	GTATCC	1 cut(s)	105
BglI	GCCNNNNNGGC	1 cut(s)	1177
BisI	GCNGC	4 cut(s)	150, 153, 858, 1169
BlsI	GCNGC	4 cut(s)	151, 154, 859, 1170
Bme1390I	CCNGG	2 cut(s)	354, 466
Bme18I	GGWCC	3 cut(s)	350, 573, 840
BmeT110I	CYCGRG	2 cut(s)	675, 843
BmgT120I	GGNCC	4 cut(s)	350, 573, 653, 840
BmiI	GGNNCC	2 cut(s)	574, 842
BmrFI	CCNGG	2 cut(s)	354, 466
BmsI	GCATC	6 cut(s)	59, 132, 577, 676, 983, 1280
BpiI	GAAGAC	2 cut(s)	362, 764
BplI	GAGNNNNNCTC	2 cut(s)	1201, 1233
Bpu10I	CCTNAGC	1 cut(s)	1200
BpuEI	CTTGAG	2 cut(s)	658, 747
BpuMI	CCSGG	1 cut(s)	354
BsaJI	CCNNGG	1 cut(s)	1172
BsaXI	ACNNNNNCTCC	4 cut(s)	73, 103, 634, 664
Bsc4I	CCNNNNNNNGG	1 cut(s)	428
Bse1I	ACTGG	1 cut(s)	1093
BseBI	CCWGG	1 cut(s)	466
BseDI	CCNNGG	1 cut(s)	1172
BseGI	GGATG	2 cut(s)	534, 1243
BseLI	CCNNNNNNNGG	1 cut(s)	428
BseMII	CTCAG	3 cut(s)	196, 215, 1191
BseNI	ACTGG	1 cut(s)	1093
BseRI	GAGGAG	2 cut(s)	655, 1035
BseXI	GCAGC	1 cut(s)	844
BseYI	CCCAGC	1 cut(s)	1049
BshFI	GGCC	3 cut(s)	655, 1171, 1180
BsiHKCI	CYCGRG	2 cut(s)	675, 843
BsiSI	CCGG	1 cut(s)	353
BsiWI	CGTACG	1 cut(s)	744
BslFI	GGGAC	2 cut(s)	586, 826
BslI	CCNNNNNNNGG	1 cut(s)	428
BsmAI	GTCTC	1 cut(s)	1254
BsmBI	CGTCTC	1 cut(s)	1254
BsmFI	GGGAC	2 cut(s)	586, 826
BsmI	GAATGC	1 cut(s)	1239
BsnI	GGCC	3 cut(s)	655, 1171, 1180
BsoBI	CYCGRG	2 cut(s)	675, 843
Bsp1286I	GDGCHC	1 cut(s)	997
Bsp1407I	TGTACA	3 cut(s)	433, 562, 1058
Bsp143I	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
BspACI	CCGC	7 cut(s)	48, 147, 150, 153, 385, 1168, 1237
BspANI	GGCC	3 cut(s)	655, 1171, 1180
BspCNI	CTCAG	3 cut(s)	195, 214, 1192
BspLI	GGNNCC	2 cut(s)	574, 842
BspMAI	CTGCAG	2 cut(s)	334, 1138
BspMI	ACCTGC	1 cut(s)	1141
BspPI	GGATC	1 cut(s)	1115
BspQI	GCTCTTC	1 cut(s)	660
BsrGI	TGTACA	3 cut(s)	433, 562, 1058
BsrI	ACTGG	1 cut(s)	1093
BssECI	CCNNGG	1 cut(s)	1172
BssMI	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
BssSI	CACGAG	1 cut(s)	543
BssT1I	CCWWGG	1 cut(s)	1172
Bst2BI	CACGAG	1 cut(s)	543
Bst2UI	CCWGG	1 cut(s)	466
Bst4CI	ACNGT	2 cut(s)	173, 1070
Bst6I	CTCTTC	1 cut(s)	660
BstAUI	TGTACA	3 cut(s)	433, 562, 1058
BstC8I	GCNNGC	5 cut(s)	216, 657, 672, 774, 1237
BstDEI	CTNAG	3 cut(s)	182, 201, 1200
BstF5I	GGATG	2 cut(s)	534, 1243
BstH2I	RGCGCY	1 cut(s)	296
BstHHI	GCGC	2 cut(s)	259, 295
BstKTI	GATC	6 cut(s)	109, 277, 550, 1006, 1123, 1165
BstMAI	GTCTC	1 cut(s)	1254
BstMBI	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
BstMWI	GCNNNNNNNGC	5 cut(s)	254, 292, 461, 854, 1177
BstNI	CCWGG	1 cut(s)	466
BstSCI	CCNGG	2 cut(s)	352, 464
BstSFI	CTRYAG	4 cut(s)	330, 660, 1134, 1289
BstV1I	GCAGC	1 cut(s)	844
BstV2I	GAAGAC	2 cut(s)	362, 764
BsuI	GTATCC	1 cut(s)	105
BsuRI	GGCC	3 cut(s)	655, 1171, 1180
BtsCI	GGATG	2 cut(s)	534, 1243
BveI	ACCTGC	1 cut(s)	1141
Cac8I	GCNNGC	5 cut(s)	216, 657, 672, 774, 1237
CaiI	CAGNNNCTG	1 cut(s)	338
CfoI	GCGC	2 cut(s)	259, 295
Cfr13I	GGNCC	4 cut(s)	350, 573, 653, 840
Csp6I	GTAC	6 cut(s)	408, 434, 530, 563, 745, 1059
CviAII	CATG	4 cut(s)	104, 442, 452, 1083
CviQI	GTAC	6 cut(s)	408, 434, 530, 563, 745, 1059
DdeI	CTNAG	3 cut(s)	182, 201, 1200
DpnI	GATC	6 cut(s)	108, 276, 549, 1005, 1122, 1164
DpnII	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
EaeI	YGGCCR	2 cut(s)	1169, 1178
Eam1104I	CTCTTC	1 cut(s)	660
EarI	CTCTTC	1 cut(s)	660
Eco130I	CCWWGG	1 cut(s)	1172
Eco24I	GRGCYC	1 cut(s)	997
Eco47I	GGWCC	3 cut(s)	350, 573, 840
Eco47III	AGCGCT	1 cut(s)	294
Eco57I	CTGAAG	3 cut(s)	776, 801, 1167
Eco88I	CYCGRG	2 cut(s)	675, 843
EcoO109I	RGGNCCY	1 cut(s)	840
EcoRII	CCWGG	1 cut(s)	464
EcoT14I	CCWWGG	1 cut(s)	1172
EcoT38I	GRGCYC	1 cut(s)	997
ErhI	CCWWGG	1 cut(s)	1172
Esp3I	CGTCTC	1 cut(s)	1254
FaeI	CATG	4 cut(s)	107, 445, 455, 1086
FalI	AAGNNNNNCTT	2 cut(s)	279, 311
FaqI	GGGAC	2 cut(s)	586, 826
FatI	CATG	4 cut(s)	103, 441, 451, 1082
FauI	CCCGC	1 cut(s)	1244
FauNDI	CATATG	2 cut(s)	604, 951
FbaI	TGATCA	1 cut(s)	1003
Fnu4HI	GCNGC	4 cut(s)	150, 153, 858, 1169
FokI	GGATG	2 cut(s)	521, 1230
FriOI	GRGCYC	1 cut(s)	997
Fsp4HI	GCNGC	4 cut(s)	150, 153, 858, 1169
FspBI	CTAG	2 cut(s)	71, 825
GlaI	GCGC	2 cut(s)	258, 294
GluI	GCNGC	4 cut(s)	150, 153, 858, 1169
GsaI	CCCAGC	1 cut(s)	1053
HaeII	RGCGCY	1 cut(s)	296
HaeIII	GGCC	3 cut(s)	655, 1171, 1180
HapII	CCGG	1 cut(s)	353
HhaI	GCGC	2 cut(s)	259, 295
Hin1II	CATG	4 cut(s)	107, 445, 455, 1086
Hin6I	GCGC	2 cut(s)	257, 293
HinP1I	GCGC	2 cut(s)	257, 293
HincII	GTYRAC	1 cut(s)	471
HindII	GTYRAC	1 cut(s)	471
HinfI	GANTC	4 cut(s)	58, 709, 760, 782
HpaI	GTTAAC	1 cut(s)	471
HpaII	CCGG	1 cut(s)	353
HphI	GGTGA	3 cut(s)	185, 608, 695
Hpy166II	GTNNAC	3 cut(s)	434, 471, 905
Hpy188I	TCNGA	3 cut(s)	756, 870, 1254
Hpy188III	TCNNGA	9 cut(s)	71, 160, 516, 545, 632, 706, 843, 890, 1015
Hpy8I	GTNNAC	3 cut(s)	434, 471, 905
Hpy99I	CGWCG	1 cut(s)	161
HpyAV	CCTTC	3 cut(s)	831, 948, 1200
HpyCH4III	ACNGT	2 cut(s)	173, 1070
HpyCH4V	TGCA	9 cut(s)	265, 286, 332, 689, 735, 878, 974, 1136, 1279
HpyF10VI	GCNNNNNNNGC	5 cut(s)	254, 292, 461, 854, 1177
HpyF3I	CTNAG	3 cut(s)	182, 201, 1200
Hsp92II	CATG	4 cut(s)	107, 445, 455, 1086
HspAI	GCGC	2 cut(s)	257, 293
Ksp22I	TGATCA	1 cut(s)	1003
KspAI	GTTAAC	1 cut(s)	471
Kzo9I	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
LguI	GCTCTTC	1 cut(s)	660
LmnI	GCTCC	1 cut(s)	241
Lsp1109I	GCAGC	1 cut(s)	844
LweI	GCATC	6 cut(s)	59, 132, 577, 676, 983, 1280
MaeI	CTAG	2 cut(s)	71, 825
MaeIII	GTNAC	2 cut(s)	137, 340
MalI	GATC	6 cut(s)	108, 276, 549, 1005, 1122, 1164
MboI	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
MfeI	CAATTG	2 cut(s)	777, 912
MhlI	GDGCHC	1 cut(s)	997
MlsI	TGGCCA	1 cut(s)	1180
MluNI	TGGCCA	1 cut(s)	1180
MlyI	GAGTC	2 cut(s)	703, 754
MmeI	TCCRAC	1 cut(s)	63
Mox20I	TGGCCA	1 cut(s)	1180
MscI	TGGCCA	1 cut(s)	1180
MseI	TTAA	4 cut(s)	311, 359, 470, 795
Msp20I	TGGCCA	1 cut(s)	1180
MspA1I	CMGCKG	2 cut(s)	135, 659
MspI	CCGG	1 cut(s)	353
MspR9I	CCNGG	2 cut(s)	354, 466
MunI	CAATTG	2 cut(s)	777, 912
Mva1269I	GAATGC	1 cut(s)	1239
MvaI	CCWGG	1 cut(s)	466
MwoI	GCNNNNNNNGC	5 cut(s)	254, 292, 461, 854, 1177
NciI	CCSGG	1 cut(s)	354
NdeI	CATATG	2 cut(s)	604, 951
NdeII	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
NlaIII	CATG	4 cut(s)	107, 445, 455, 1086
NlaIV	GGNNCC	2 cut(s)	574, 842
NmuCI	GTSAC	1 cut(s)	137
PaqCI	CACCTGC	1 cut(s)	1141
PciSI	GCTCTTC	1 cut(s)	660
PctI	GAATGC	1 cut(s)	1239
PfeI	GAWTC	2 cut(s)	58, 782
Pfl23II	CGTACG	1 cut(s)	744
PflMI	CCANNNNNTGG	1 cut(s)	428
PkrI	GCNGC	4 cut(s)	151, 154, 859, 1170
PleI	GAGTC	2 cut(s)	703, 754
PpsI	GAGTC	2 cut(s)	703, 754
PpuMI	RGGWCCY	1 cut(s)	840
PshBI	ATTAAT	1 cut(s)	795
Psp5II	RGGWCCY	1 cut(s)	840
Psp6I	CCWGG	1 cut(s)	464
PspFI	CCCAGC	1 cut(s)	1049
PspGI	CCWGG	1 cut(s)	464
PspLI	CGTACG	1 cut(s)	744
PspN4I	GGNNCC	2 cut(s)	574, 842
PspPI	GGNCC	4 cut(s)	350, 573, 653, 840
PspPPI	RGGWCCY	1 cut(s)	840
PsrI	GAACNNNNNNTAC	2 cut(s)	468, 500
PstI	CTGCAG	2 cut(s)	334, 1138
PstNI	CAGNNNCTG	1 cut(s)	338
PvuII	CAGCTG	2 cut(s)	135, 659
RsaI	GTAC	6 cut(s)	409, 435, 531, 564, 746, 1060
RsaNI	GTAC	6 cut(s)	408, 434, 530, 563, 745, 1059
SapI	GCTCTTC	1 cut(s)	660
SaqAI	TTAA	4 cut(s)	311, 359, 470, 795
SatI	GCNGC	4 cut(s)	150, 153, 858, 1169
Sau3AI	GATC	6 cut(s)	106, 274, 547, 1003, 1120, 1162
Sau96I	GGNCC	4 cut(s)	350, 573, 653, 840
SchI	GAGTC	2 cut(s)	703, 754
ScrFI	CCNGG	2 cut(s)	354, 466
SduI	GDGCHC	1 cut(s)	997
SfaNI	GCATC	6 cut(s)	59, 132, 577, 676, 983, 1280
SfcI	CTRYAG	4 cut(s)	330, 660, 1134, 1289
SfiI	GGCCNNNNNGGCC	1 cut(s)	1177
SinI	GGWCC	3 cut(s)	350, 573, 840
SmlI	CTYRAG	2 cut(s)	637, 762
SmoI	CTYRAG	2 cut(s)	637, 762
SsiI	CCGC	7 cut(s)	48, 147, 150, 153, 385, 1168, 1237
SspI	AATATT	1 cut(s)	793
SspMI	CTAG	2 cut(s)	71, 825
StyD4I	CCNGG	2 cut(s)	352, 464
StyI	CCWWGG	1 cut(s)	1172
TaaI	ACNGT	2 cut(s)	173, 1070
TaqI	TCGA	3 cut(s)	85, 126, 707
TaqII	GACCGA	1 cut(s)	1209
TatI	WGTACW	4 cut(s)	433, 529, 562, 1058
TauI	GCSGC	3 cut(s)	152, 155, 1171
TfiI	GAWTC	2 cut(s)	58, 782
Tru1I	TTAA	4 cut(s)	311, 359, 470, 795
Tru9I	TTAA	4 cut(s)	311, 359, 470, 795
TseFI	GTSAC	1 cut(s)	137
TseI	GCWGC	1 cut(s)	857
Tsp45I	GTSAC	1 cut(s)	137
TspDTI	ATGAA	3 cut(s)	731, 1155, 1232
TspGWI	ACGGA	2 cut(s)	81, 1236
Van91I	CCANNNNNTGG	1 cut(s)	428
VpaK11BI	GGWCC	3 cut(s)	350, 573, 840
VspI	ATTAAT	1 cut(s)	795
XapI	RAATTY	3 cut(s)	31, 177, 701
XbaI	TCTAGA	1 cut(s)	70
XspI	CTAG	2 cut(s)	71, 825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.