Rroxscaffold_4G00311970

Mitochondrial ribosome-associated GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017706
Physical Location & Seq
Reverse (-)
34598329 .. 34606171
7843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311970.1

Sequence Viewer

Length: 1731 bp
ATGAATGAGAATTCTGAAACATTTGAGATACATATATTCATGTTTTCATTTTCGAATGCAGGAACCATTATAGATTGTTATGCTGGGGAACAAGAACTAGCTCAGTGTTTTCTGGCTATCTTGAACAGAAGCAACGAATACAAGAAATGGGCAAATTTGTCCAACAGTGAGAATGGTAGAACATTGATAGACCACAGTATAGAATTCTCAAATAGCTCGAAAGACATCAAACGGAAAAGAAGATACTCTGCAGATCACACACAGGATTTCATAGTGCAAGATGTTCGTCAGATTCTCTTCAAAATTATATCATCTTTCAATGGTGACATGGAGGATGAAATGCTTATTGAGGAACAGATCAAGGCCCTGCAAGGAGAAATCAAAAGTGGTGGCGAACAATCAAGTGAGACAACATCTGACGAGGATACAACTGGTGATCATGAGAAAAAGAATTCCAAGAGAAGAAAATCAACAAAGGAAGGCAATGCCCCGAAAAAGGCAAAGTTGATATGGACGCAAGAGCTCCAGGCTCGGTTTCTGGAGTCTGTTCATTTTATAGGCCTAGAAAGGGCTGTTCCGAAAACAATTCTTGAGGTCATGAACGTTGAAGGCCTGACTAGAGAAAACGTGGCGAGCCATTTGCAGAAGTATAGACAATTTTTAAGGAGAGTTGCAGTAAAGATTTCAGTAAGTAAATCGGATATTGGTAACAAGTCCCAGATGTCAAGCTCTACATTCGGGCAAAGTCAAACTTCGATATTCCAGCAGCAGCCCATTTGCGGACAGAATCTCTCCATATTAAATCTTAATGCTTCAAGCCTTGGACGTCCCCTTTTTCATGCCAATCACCACGAAGCATCAACCACTTCCGTGCCTCCATTCGGAAACACATCACACTTATATCAACCGAATTCTCTATCACATTTTCAGATGCCGCAACCTGCTTTCATGGGGTACCCAGGGTCTTTCCAACCAGCCAATTACTCATCAGTTAATGGGAGTGAATTTCAGACAAATAATGTTCAAAGCAATTGGTCAAGTGCTAATGGAGTCCAAGCTCAAACAAGTCCACTGACCAACCATCTATATGGCAATTACAATAACTATGGCATGAGTACAGGGTTTCAGTTTGGTTCATGGAATGGAACTATGGGATTTCTGAATGATTCTAATAGTCTATTCACCAACAACTACTATGGTGGACTCCCAATGAGTGGTGCTGCTTCTGTAGGGCTAGCTGGATTTAACTCGAATCAAAACCCTAATTTGATGGTGGCGAACCACGGGTGGGCTTCATCAACTAGTGGACTCGATATTAATTTTTTTGGATCGGTTCTGAATGATATTGATCAAAGTGAGCTTCATCAAGAAATGAACAATACTTCACCAACGGGTAATTTGATGTTCGGTAACAATAATTTTTCAGGCGTGGAGGATGCTTCATCAGTTGGTTTTGGTGGTATTACAACTGTGGAAGCTGCCGCTTCTAGCTCTAATGTTGTAGACGTATCACCAAATCAGAATCAAACCGGCGAGTGCTTTGATGAACTTCATGATATCTTATTCAACGATACCAATTTGAAATCTGTCAACCAGCAGAATTTTAATAGAGAACTCAACGAAGAACTGGAGTTTGACTGGTTGGATCTATTGGATGCCACAGCAATTCTAGATAATGTCTCCTTAGATGAGGAATGCAAGAAGTTAATTGACTCAATCCTCAACCACTGA

Protein Analysis

576

Amino Acids

63.88

Weight (kDa)

5.02

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g07970
malus_domestica MD02G1229600.v1.1
rosa_chinensis RchiOBHm_Chr1g0341321
rosa_multiflora Rmu_sc0000047.1_g000016 Rmu_sc0037241.1_g000002
rosa_roxburghii Rroxscaffold_4G00311970
rosa_rugosa Rorug01G0157400.1
rosa_samantha Rh1AG172200 Rh1BG140000 Rh1CG160300 Rh1DG172400
rosa_wichuraiana Rw1G014360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 829
Acc36I ACCTGC 1 cut(s) 949
Acc65I GGTACC 1 cut(s) 954
AccB1I GGYRCC 1 cut(s) 954
AccB7I CCANNNNNTGG 1 cut(s) 1214
AccI GTMKAC 1 cut(s) 1503
AciI CCGC 3 cut(s) 780, 935, 1482
AclI AACGTT 1 cut(s) 603
AclWI GGATC 2 cut(s) 1336, 1653
AcsI RAATTY 7 cut(s) 10, 154, 203, 451, 910, 1004, 1600
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 2 cut(s) 956, 1117
AfiI CCNNNNNNNGG 6 cut(s) 496, 568, 779, 881, 1214, 1288
AgsI TTSAA 8 cut(s) 124, 301, 319, 608, 816, 1025, 1567, 1582
AhlI ACTAGT 1 cut(s) 1301
AjnI CCWGG 2 cut(s) 525, 958
AleI CACNNNNGTG 1 cut(s) 869
AluBI AGCT 9 cut(s) 101, 216, 523, 729, 1058, 1238, 1360, 1478, 1491
AluI AGCT 9 cut(s) 101, 216, 523, 729, 1058, 1238, 1360, 1478, 1491
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 2 cut(s) 401, 1684
AlwI GGATC 2 cut(s) 1336, 1653
AoxI GGCC 3 cut(s) 363, 559, 610
ApeKI GCWGC 4 cut(s) 766, 769, 1220, 1478
ApoI RAATTY 7 cut(s) 10, 154, 203, 451, 910, 1004, 1600
AseI ATTAAT 1 cut(s) 1317
Asp718I GGTACC 1 cut(s) 954
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 6 cut(s) 335, 446, 839, 1174, 1377, 1503
AsuII TTCGAA 1 cut(s) 53
AsuNHI GCTAGC 1 cut(s) 1234
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 4 cut(s) 778, 781, 1207, 1465
BccI CCATC 2 cut(s) 1089, 1264
BcgI CGANNNNNNTGC 4 cut(s) 266, 300, 622, 656
BciT130I CCWGG 2 cut(s) 527, 960
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 2 cut(s) 436, 1348
BcoDI GTCTC 2 cut(s) 401, 1684
BcuI ACTAGT 1 cut(s) 1301
BfaI CTAG 7 cut(s) 98, 563, 618, 1235, 1302, 1488, 1670
BfmI CTRYAG 2 cut(s) 249, 1227
BfuAI ACCTGC 1 cut(s) 949
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 6 cut(s) 767, 770, 935, 1221, 1479, 1482
BlsI GCNGC 6 cut(s) 768, 771, 936, 1222, 1480, 1483
Bme1390I CCNGG 2 cut(s) 527, 960
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 2 cut(s) 64, 956
BmrFI CCNGG 2 cut(s) 527, 960
BmsI GCATC 4 cut(s) 866, 921, 1426, 1645
BmtI GCTAGC 1 cut(s) 1238
BpmI CTGGAG 3 cut(s) 509, 560, 1649
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 1347
BsaHI GRCGYC 1 cut(s) 826
BsaJI CCNNGG 4 cut(s) 820, 958, 959, 1282
Bsc4I CCNNNNNNNGG 6 cut(s) 496, 568, 779, 881, 1214, 1288
Bse118I RCCGGY 1 cut(s) 1529
Bse1I ACTGG 3 cut(s) 436, 1632, 1643
Bse3DI GCAATG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 2 cut(s) 527, 960
BseDI CCNNGG 4 cut(s) 820, 958, 959, 1282
BseGI GGATG 3 cut(s) 340, 1441, 1660
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 6 cut(s) 496, 568, 779, 881, 1214, 1288
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 3 cut(s) 436, 1632, 1643
BseXI GCAGC 4 cut(s) 778, 781, 1207, 1465
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 3 cut(s) 365, 561, 612
BshNI GGYRCC 1 cut(s) 954
BsiHKAI GWGCWC 1 cut(s) 525
BsiSI CCGG 1 cut(s) 1530
BslFI GGGAC 2 cut(s) 700, 813
BslI CCNNNNNNNGG 6 cut(s) 496, 568, 779, 881, 1214, 1288
BsmAI GTCTC 2 cut(s) 401, 1684
BsmFI GGGAC 2 cut(s) 700, 813
BsmI GAATGC 2 cut(s) 61, 1700
BsnI GGCC 3 cut(s) 365, 561, 612
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
BspACI CCGC 3 cut(s) 780, 935, 1482
BspANI GGCC 3 cut(s) 365, 561, 612
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 3 cut(s) 439, 597, 1552
BspLI GGNNCC 2 cut(s) 64, 956
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 949
BspOI GCTAGC 1 cut(s) 1238
BspPI GGATC 2 cut(s) 1336, 1653
BspT104I TTCGAA 1 cut(s) 53
BspT107I GGYRCC 1 cut(s) 954
BsrDI GCAATG 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 1529
BsrI ACTGG 3 cut(s) 436, 1632, 1643
BssAI RCCGGY 1 cut(s) 1529
BssECI CCNNGG 4 cut(s) 820, 958, 959, 1282
BssMI GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 1 cut(s) 820
Bst2UI CCWGG 2 cut(s) 527, 960
Bst4CI ACNGT 3 cut(s) 167, 197, 1471
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 826
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 634, 1236
BstDEI CTNAG 2 cut(s) 102, 1684
BstDSI CCRYGG 1 cut(s) 1282
BstF5I GGATG 3 cut(s) 340, 1441, 1660
BstKTI GATC 6 cut(s) 256, 360, 439, 1331, 1351, 1648
BstMAI GTCTC 2 cut(s) 401, 1684
BstMBI GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
BstNI CCWGG 2 cut(s) 527, 960
BstSCI CCNGG 2 cut(s) 525, 958
BstSFI CTRYAG 2 cut(s) 249, 1227
BstV1I GCAGC 4 cut(s) 778, 781, 1207, 1465
BstX2I RGATCY 1 cut(s) 1645
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 1 cut(s) 1645
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 3 cut(s) 365, 561, 612
BtgI CCRYGG 1 cut(s) 1282
BtsCI GGATG 3 cut(s) 340, 1441, 1660
BtsIMutI CAGTG 4 cut(s) 110, 172, 1070, 1726
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 2 cut(s) 634, 1236
CciI TCATGA 3 cut(s) 439, 597, 1552
Cfr10I RCCGGY 1 cut(s) 1529
Cfr13I GGNCC 1 cut(s) 364
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 955, 1116
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 9 cut(s) 40, 328, 440, 598, 839, 949, 1111, 1137, 1553
CviQI GTAC 2 cut(s) 955, 1116
DdeI CTNAG 2 cut(s) 102, 1684
DpnI GATC 6 cut(s) 255, 359, 438, 1330, 1350, 1647
DpnII GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 820
Eco147I AGGCCT 2 cut(s) 561, 612
Eco24I GRGCYC 1 cut(s) 525
Eco32I GATATC 1 cut(s) 1558
Eco53kI GAGCTC 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 364
EcoRI GAATTC 4 cut(s) 10, 203, 451, 910
EcoRII CCWGG 2 cut(s) 525, 958
EcoRV GATATC 1 cut(s) 1558
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 9 cut(s) 43, 331, 443, 601, 842, 952, 1114, 1140, 1556
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FaqI GGGAC 2 cut(s) 700, 813
FatI CATG 9 cut(s) 39, 327, 439, 597, 838, 948, 1110, 1136, 1552
FbaI TGATCA 2 cut(s) 436, 1348
FblI GTMKAC 1 cut(s) 1503
Fnu4HI GCNGC 6 cut(s) 767, 770, 935, 1221, 1479, 1482
FokI GGATG 3 cut(s) 347, 1448, 1667
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 6 cut(s) 767, 770, 935, 1221, 1479, 1482
FspBI CTAG 7 cut(s) 98, 563, 618, 1235, 1302, 1488, 1670
GluI GCNGC 6 cut(s) 767, 770, 935, 1221, 1479, 1482
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 3 cut(s) 509, 560, 1649
HaeIII GGCC 3 cut(s) 365, 561, 612
HapII CCGG 1 cut(s) 1530
HgaI GACGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 9 cut(s) 43, 331, 443, 601, 842, 952, 1114, 1140, 1556
HincII GTYRAC 1 cut(s) 1591
HindII GTYRAC 1 cut(s) 1591
HpaII CCGG 1 cut(s) 1530
HphI GGTGA 6 cut(s) 335, 446, 839, 1174, 1377, 1503
Hpy166II GTNNAC 5 cut(s) 1070, 1202, 1307, 1504, 1591
Hpy188III TCNNGA 8 cut(s) 121, 440, 539, 590, 598, 1367, 1553, 1670
Hpy8I GTNNAC 5 cut(s) 1070, 1202, 1307, 1504, 1591
HpyAV CCTTC 2 cut(s) 473, 602
HpyCH4III ACNGT 3 cut(s) 167, 197, 1471
HpyCH4IV ACGT 4 cut(s) 603, 627, 826, 1506
HpyCH4V TGCA 7 cut(s) 59, 251, 277, 370, 643, 674, 1698
HpyF3I CTNAG 2 cut(s) 102, 1684
HpySE526I ACGT 4 cut(s) 603, 627, 826, 1506
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 9 cut(s) 43, 331, 443, 601, 842, 952, 1114, 1140, 1556
KpnI GGTACC 1 cut(s) 958
Ksp22I TGATCA 2 cut(s) 436, 1348
Kzo9I GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
LmnI GCTCC 1 cut(s) 528
Lsp1109I GCAGC 4 cut(s) 778, 781, 1207, 1465
LweI GCATC 4 cut(s) 866, 921, 1426, 1645
MaeI CTAG 7 cut(s) 98, 563, 618, 1235, 1302, 1488, 1670
MaeII ACGT 4 cut(s) 603, 627, 826, 1506
MaeIII GTNAC 3 cut(s) 323, 707, 1409
MalI GATC 6 cut(s) 255, 359, 438, 1330, 1350, 1647
MboI GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
MboII GAAGA 4 cut(s) 252, 289, 474, 1634
MfeI CAATTG 1 cut(s) 1030
MflI RGATCY 1 cut(s) 1645
MhlI GDGCHC 1 cut(s) 525
MlyI GAGTC 5 cut(s) 551, 1059, 1197, 1302, 1706
MmeI TCCRAC 3 cut(s) 186, 994, 1623
MnlI CCTC 8 cut(s) 325, 343, 415, 586, 885, 1426, 1684, 1730
MseI TTAA 8 cut(s) 662, 800, 807, 993, 1245, 1317, 1605, 1706
MslI CAYNNNNRTG 2 cut(s) 869, 1086
MspI CCGG 1 cut(s) 1530
MspR9I CCNGG 2 cut(s) 527, 960
MunI CAATTG 1 cut(s) 1030
Mva1269I GAATGC 2 cut(s) 61, 1700
MvaI CCWGG 2 cut(s) 527, 960
NdeII GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
NheI GCTAGC 1 cut(s) 1234
NlaIII CATG 9 cut(s) 43, 331, 443, 601, 842, 952, 1114, 1140, 1556
NlaIV GGNNCC 2 cut(s) 64, 956
NmuCI GTSAC 1 cut(s) 323
NspV TTCGAA 1 cut(s) 53
OliI CACNNNNGTG 1 cut(s) 869
PagI TCATGA 3 cut(s) 439, 597, 1552
PasI CCCWGGG 1 cut(s) 959
PceI AGGCCT 2 cut(s) 561, 612
PctI GAATGC 2 cut(s) 61, 1700
PfeI GAWTC 5 cut(s) 292, 787, 1166, 1252, 1522
PflMI CCANNNNNTGG 1 cut(s) 1214
PkrI GCNGC 6 cut(s) 768, 771, 936, 1222, 1480, 1483
PleI GAGTC 5 cut(s) 550, 1058, 1197, 1302, 1706
PpsI GAGTC 5 cut(s) 550, 1058, 1197, 1302, 1706
PshBI ATTAAT 1 cut(s) 1317
Psp124BI GAGCTC 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 603
Psp6I CCWGG 2 cut(s) 525, 958
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 2 cut(s) 525, 958
PspN4I GGNNCC 2 cut(s) 64, 956
PspPI GGNCC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 253
PsuI RGATCY 1 cut(s) 1645
RsaI GTAC 2 cut(s) 956, 1117
RsaNI GTAC 2 cut(s) 955, 1116
RseI CAYNNNNRTG 2 cut(s) 869, 1086
SacI GAGCTC 1 cut(s) 525
SaqAI TTAA 8 cut(s) 662, 800, 807, 993, 1245, 1317, 1605, 1706
SatI GCNGC 6 cut(s) 767, 770, 935, 1221, 1479, 1482
Sau3AI GATC 6 cut(s) 253, 357, 436, 1328, 1348, 1645
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 5 cut(s) 551, 1059, 1197, 1302, 1706
ScrFI CCNGG 2 cut(s) 527, 960
SduI GDGCHC 1 cut(s) 525
SfaNI GCATC 4 cut(s) 866, 921, 1426, 1645
SfcI CTRYAG 2 cut(s) 249, 1227
SfuI TTCGAA 1 cut(s) 53
SmiMI CAYNNNNRTG 2 cut(s) 869, 1086
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SpeI ACTAGT 1 cut(s) 1301
SseBI AGGCCT 2 cut(s) 561, 612
SsiI CCGC 3 cut(s) 780, 935, 1482
SspMI CTAG 7 cut(s) 98, 563, 618, 1235, 1302, 1488, 1670
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 2 cut(s) 561, 612
StyD4I CCNGG 2 cut(s) 525, 958
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 3 cut(s) 167, 197, 1471
TaiI ACGT 4 cut(s) 606, 630, 829, 1509
TaqI TCGA 5 cut(s) 53, 218, 755, 1250, 1311
TatI WGTACW 1 cut(s) 1115
TauI GCSGC 2 cut(s) 937, 1484
TfiI GAWTC 5 cut(s) 292, 787, 1166, 1252, 1522
Tru1I TTAA 8 cut(s) 662, 800, 807, 993, 1245, 1317, 1605, 1706
Tru9I TTAA 8 cut(s) 662, 800, 807, 993, 1245, 1317, 1605, 1706
TscAI CASTG 3 cut(s) 110, 172, 1077
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 4 cut(s) 766, 769, 1220, 1478
Tsp45I GTSAC 1 cut(s) 323
TspGWI ACGGA 2 cut(s) 247, 859
TspRI CASTG 3 cut(s) 110, 172, 1077
Van91I CCANNNNNTGG 1 cut(s) 1214
VspI ATTAAT 1 cut(s) 1317
XapI RAATTY 7 cut(s) 10, 154, 203, 451, 910, 1004, 1600
XbaI TCTAGA 1 cut(s) 1669
XmiI GTMKAC 1 cut(s) 1503
XspI CTAG 7 cut(s) 98, 563, 618, 1235, 1302, 1488, 1670
ZraI GACGTC 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.