Rroxscaffold_4G00331660

Protein gamma response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007401
Physical Location & Seq
Forward (+)
65623120 .. 65626071
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331660.1

Sequence Viewer

Length: 1803 bp
ATGGAGGAGACTACCAGACAAATTTGTTTCCCTATTGATACTCAATCTCCTGATATCAAGCTTGCTACTCTACTACTTGCTACCATTCAGGAAGCTAAAGACCGGATTCAGGAAACTAAAGACCGGGTTTACCAGATCGAATATGTCTTCCTTACTCAGCTATACCCCTTATTGCAGTCCAAGTCCGAAACTCTCCAGAAATTTCAAGATGAGTGGAAATCCAAGGAAACTAATCTCTTGCTTCAAATTGAAACACTTCGAGCTGAAAACGAACTAATCGTTGAAGAGAACTGCTCACTCAAGCTTGACAGGGAAAAGCTGTTCAGAGAAAAGGAAGACGTGATAAACCAACTGCGAGATGAAGCGACAGGCCTACAACTAAGGAGTGATGAGCTGGAACAAATGCTTGACCACAAGTCTAAGGAAGCCGATGAGGGAATGGAATTGCGGAGTAAGTTGCTCGAAGAGGTTGAATGGAAAGACAGTGTCATTGTGAGTAAGGAAAAACAACTGAAAGAGAGTGAAGAAAGTAGAAATGTGCTTTCTGCGAATGTGAGTGATCTTGAAAAAAGAGCTGATGAGGTCAAACAAAATCTGTTCAAGAAGATTGAGTTACAAGCTTCTGAGATCATGAACAATGAAAAGCTTTTGAAGTATCGGGAAGAAGAGAAAAAACTAATGTTGACCAAATTGAAAAATGTGGAAGAAAATGTTAGCCAACTCCAGAAGGAGCTCCTAACAAAGAATGAAGAAGTGGAAGATTGTGCAAGAGAGAAAAAACTTCTTCTGAGAAAAATGACTGGTTTAGAGGAGAAAGTTAATGAGCTACAAGGGGATCTCAGAGGCAGGGCCGGTGAAGCTGCCAAAAGAAGGGGTTTGACTGAAAATTTACACCAACAGGTTGAATCAATAACCGCAGATTTACTGACCGAGAGGAAGAAGTACGGGGAACTTACTGCTGCTTACAAAAGTCTAAAATCTCAGCACACATATCTCCGCAGCAAATATGGTCTTACCAAGGAGAATATGCTCCCCCATAATAAACTGGAAGACGAAAGTGATTTATTGAGAAATGATCAGAATCCATCAACTTTCCATGATCTTGAAGATAAAAATCAACATGCCTCTCCAGCTGCTTGTAACACAAAGAAAATGAAGAATGAAATCAGTTTTAACAATAACTTGAATGAGGGCAAAGTACAAAAACCAATTCAAGCAACAAGTCCTTTCTCTTCTACTTCTGTTTCACCCAAATGTCCTTCCACTGTAAAATCTGCTCCAGTAGCTGGTAGAAAGCGGTCTGCATCGTCCTGGATAGACACAAGATCCCGCCAAGGCCAAAATGGGACTGACCCACACGATGATTTTCTTGACACCCCGATTGAGAACGTAAGAGCAAACTTGAATAGATCAACAAAGGAAAAAGTTACTGATCTTCCAGTTCCAGTTCAAAATGACTCAAATTTAGGTAGCTCTGATGATGAAACACAGGATGTGACTGCTGATCATCCTAGGCTACCAAAGCAGCATATGCCAGTTACAGTGGCTGGCAAAAATGGTTTCAAGTATGTGGAACCTGTAAGAAAGAGGGCTGAGCGGGAAAATTTGAAAGGAGTTGAATGCAAACAGTGCAAAAAGTTTTATGATGCTGTTCTTCCAGACGATGGTGGCAAAGACAATGATAATAATAAGCATGATTTCCGCTGTGAGCATCATGAAGGTGTTTCTCGACACAGGTACAGGTATCTTCCCCCTTCAACTCCCGAAGGATTTTGGAATATTGGATTTGAATCTGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0006950 GO:0006974 GO:0007276 GO:0008150 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0019953 GO:0022414 GO:0032501 GO:0032504 GO:0033554 GO:0044703 GO:0048609 GO:0050896 GO:0051704 GO:0051716
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAE2

Protein Analysis

600

Amino Acids

69.11

Weight (kDa)

5.76

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1286, 1664
AccBSI CCGCTC 1 cut(s) 1597
AciI CCGC 7 cut(s) 448, 915, 997, 1297, 1330, 1597, 1702
AclWI GGATC 2 cut(s) 843, 1320
AcsI RAATTY 5 cut(s) 21, 200, 886, 1462, 1603
AfaI GTAC 3 cut(s) 944, 1200, 1739
AfiI CCNNNNNNNGG 4 cut(s) 109, 870, 1286, 1664
AhdI GACNNNNNGTC 1 cut(s) 415
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 1310
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 2 cut(s) 843, 1320
AlwNI CAGNNNCTG 2 cut(s) 1286, 1547
AoxI GGCC 3 cut(s) 370, 849, 1336
ApeKI GCWGC 5 cut(s) 860, 959, 999, 1133, 1525
ApoI RAATTY 5 cut(s) 21, 200, 886, 1462, 1603
Asp700I GAANNNNTTC 1 cut(s) 255
AspA2I CCTAGG 1 cut(s) 1511
AspS9I GGNCC 1 cut(s) 849
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 866, 1239
AvrII CCTAGG 1 cut(s) 1511
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 3 cut(s) 139, 342, 1056
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 5 cut(s) 847, 946, 1011, 1120, 1537
BccI CCATC 2 cut(s) 1093, 1658
BciT130I CCWGG 1 cut(s) 1312
BclI TGATCA 2 cut(s) 1075, 1504
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 1 cut(s) 1512
BisI GCNGC 5 cut(s) 861, 960, 1000, 1134, 1526
BlnI CCTAGG 1 cut(s) 1511
BlpI GCTNAGC 1 cut(s) 1593
BlsI GCNGC 5 cut(s) 862, 961, 1001, 1135, 1527
Bme1390I CCNGG 2 cut(s) 125, 1312
BmeRI GACNNNNNGTC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 849
BmiI GGNNCC 1 cut(s) 1575
BmrFI CCNGG 2 cut(s) 125, 1312
BmsI GCATC 3 cut(s) 1313, 1636, 1720
BpiI GAAGAC 3 cut(s) 139, 342, 1056
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 4 cut(s) 179, 707, 1113, 1263
Bpu1102I GCTNAGC 1 cut(s) 1593
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 3 cut(s) 1080, 1113, 1789
BsaJI CCNNGG 4 cut(s) 222, 1017, 1333, 1511
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 870, 1286, 1664
Bse118I RCCGGY 1 cut(s) 851
Bse1I ACTGG 6 cut(s) 805, 1050, 1280, 1439, 1445, 1535
Bse8I GATNNNNATC 3 cut(s) 1080, 1113, 1789
BseBI CCWGG 1 cut(s) 1312
BseDI CCNNGG 4 cut(s) 222, 1017, 1333, 1511
BseGI GGATG 2 cut(s) 1498, 1507
BseJI GATNNNNATC 3 cut(s) 1080, 1113, 1789
BseLI CCNNNNNNNGG 4 cut(s) 109, 870, 1286, 1664
BseMII CTCAG 6 cut(s) 170, 615, 779, 853, 995, 1584
BseNI ACTGG 6 cut(s) 805, 1050, 1280, 1439, 1445, 1535
BseRI GAGGAG 2 cut(s) 20, 824
BseXI GCAGC 5 cut(s) 847, 946, 1011, 1120, 1537
BshFI GGCC 3 cut(s) 372, 851, 1338
BsiHKAI GWGCWC 1 cut(s) 735
BsiSI CCGG 3 cut(s) 103, 124, 852
BslFI GGGAC 1 cut(s) 1360
BslI CCNNNNNNNGG 4 cut(s) 109, 870, 1286, 1664
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 1360
BsmI GAATGC 1 cut(s) 1625
BsnI GGCC 3 cut(s) 372, 851, 1338
Bsp1286I GDGCHC 1 cut(s) 735
Bsp1720I GCTNAGC 1 cut(s) 1593
BspACI CCGC 7 cut(s) 448, 915, 997, 1297, 1330, 1597, 1702
BspANI GGCC 3 cut(s) 372, 851, 1338
BspCNI CTCAG 6 cut(s) 169, 616, 780, 852, 994, 1585
BspHI TCATGA 2 cut(s) 630, 1714
BspLI GGNNCC 1 cut(s) 1575
BspPI GGATC 2 cut(s) 843, 1320
BsrBI CCGCTC 1 cut(s) 1597
BsrFI RCCGGY 1 cut(s) 851
BsrI ACTGG 6 cut(s) 805, 1050, 1280, 1439, 1445, 1535
BssAI RCCGGY 1 cut(s) 851
BssECI CCNNGG 4 cut(s) 222, 1017, 1333, 1511
BssT1I CCWWGG 4 cut(s) 222, 1017, 1333, 1511
Bst2UI CCWGG 1 cut(s) 1312
Bst4CI ACNGT 4 cut(s) 485, 1267, 1543, 1629
Bst6I CTCTTC 4 cut(s) 279, 459, 660, 1237
BstAPI GCANNNNNTGC 2 cut(s) 1531, 1629
BstC8I GCNNGC 2 cut(s) 63, 1549
BstDEI CTNAG 8 cut(s) 156, 380, 420, 624, 788, 839, 981, 1593
BstF5I GGATG 2 cut(s) 1498, 1507
BstMAI GTCTC 1 cut(s) 2
BstMWI GCNNNNNNNGC 6 cut(s) 857, 1130, 1283, 1522, 1531, 1629
BstNI CCWGG 1 cut(s) 1312
BstNSI RCATGY 1 cut(s) 1124
BstSCI CCNGG 2 cut(s) 123, 1310
BstV1I GCAGC 5 cut(s) 847, 946, 1011, 1120, 1537
BstV2I GAAGAC 3 cut(s) 139, 342, 1056
BstX2I RGATCY 2 cut(s) 835, 1325
BstYI RGATCY 2 cut(s) 835, 1325
BsuRI GGCC 3 cut(s) 372, 851, 1338
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 1498, 1507
BtsIMutI CAGTG 4 cut(s) 490, 1263, 1548, 1634
Cac8I GCNNGC 2 cut(s) 63, 1549
CaiI CAGNNNCTG 2 cut(s) 1286, 1547
CciI TCATGA 2 cut(s) 630, 1714
Cfr10I RCCGGY 1 cut(s) 851
Cfr13I GGNCC 1 cut(s) 849
Csp6I GTAC 3 cut(s) 943, 1199, 1738
CspCI CAANNNNNGTGG 2 cut(s) 194, 229
CviAII CATG 5 cut(s) 631, 1097, 1121, 1694, 1715
CviQI GTAC 3 cut(s) 943, 1199, 1738
DdeI CTNAG 8 cut(s) 156, 380, 420, 624, 788, 839, 981, 1593
DriI GACNNNNNGTC 1 cut(s) 415
Eam1104I CTCTTC 4 cut(s) 279, 459, 660, 1237
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 4 cut(s) 279, 459, 660, 1237
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 4 cut(s) 222, 1017, 1333, 1511
Eco147I AGGCCT 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 735
Eco32I GATATC 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 1310
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 4 cut(s) 222, 1017, 1333, 1511
EcoT38I GRGCYC 1 cut(s) 735
ErhI CCWWGG 4 cut(s) 222, 1017, 1333, 1511
FaeI CATG 5 cut(s) 634, 1100, 1124, 1697, 1718
FaqI GGGAC 1 cut(s) 1360
FatI CATG 5 cut(s) 630, 1096, 1120, 1693, 1714
FauI CCCGC 2 cut(s) 1337, 1590
FauNDI CATATG 1 cut(s) 1530
FbaI TGATCA 2 cut(s) 1075, 1504
Fnu4HI GCNGC 5 cut(s) 861, 960, 1000, 1134, 1526
FokI GGATG 2 cut(s) 1494, 1505
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 5 cut(s) 861, 960, 1000, 1134, 1526
FspBI CTAG 1 cut(s) 1512
GluI GCNGC 5 cut(s) 861, 960, 1000, 1134, 1526
GsuI CTGGAG 4 cut(s) 179, 707, 1113, 1263
HaeIII GGCC 3 cut(s) 372, 851, 1338
HapII CCGG 3 cut(s) 103, 124, 852
Hin1II CATG 5 cut(s) 634, 1100, 1124, 1697, 1718
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HindIII AAGCTT 4 cut(s) 59, 302, 618, 644
HinfI GANTC 5 cut(s) 106, 905, 1081, 1457, 1790
HpaII CCGG 3 cut(s) 103, 124, 852
HphI GGTGA 2 cut(s) 866, 1239
Hpy166II GTNNAC 2 cut(s) 130, 684
Hpy188I TCNGA 8 cut(s) 187, 326, 625, 789, 842, 1080, 1477, 1795
Hpy8I GTNNAC 2 cut(s) 130, 684
HpyAV CCTTC 6 cut(s) 721, 864, 1269, 1712, 1760, 1764
HpyCH4III ACNGT 4 cut(s) 485, 1267, 1543, 1629
HpyCH4IV ACGT 2 cut(s) 339, 1389
HpyCH4V TGCA 5 cut(s) 175, 767, 1304, 1623, 1632
HpyF10VI GCNNNNNNNGC 6 cut(s) 857, 1130, 1283, 1522, 1531, 1629
HpyF3I CTNAG 8 cut(s) 156, 380, 420, 624, 788, 839, 981, 1593
HpySE526I ACGT 2 cut(s) 339, 1389
Hsp92II CATG 5 cut(s) 634, 1100, 1124, 1697, 1718
Ksp22I TGATCA 2 cut(s) 1075, 1504
LmnI GCTCC 4 cut(s) 730, 738, 1035, 1282
Lsp1109I GCAGC 5 cut(s) 847, 946, 1011, 1120, 1537
LweI GCATC 3 cut(s) 1313, 1636, 1720
MaeI CTAG 1 cut(s) 1512
MaeII ACGT 2 cut(s) 339, 1389
MaeIII GTNAC 5 cut(s) 612, 1139, 1426, 1495, 1537
MbiI CCGCTC 1 cut(s) 1597
MflI RGATCY 2 cut(s) 835, 1325
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 9 cut(s) 21, 200, 246, 443, 689, 886, 1209, 1462, 1603
MlyI GAGTC 1 cut(s) 1451
MnlI CCTC 9 cut(s) 427, 460, 574, 802, 836, 927, 1135, 1183, 1581
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 2 cut(s) 819, 1173
MslI CAYNNNNRTG 2 cut(s) 1252, 1719
MspA1I CMGCKG 2 cut(s) 1133, 1704
MspI CCGG 3 cut(s) 103, 124, 852
MspR9I CCNGG 2 cut(s) 125, 1312
Mva1269I GAATGC 1 cut(s) 1625
MvaI CCWGG 1 cut(s) 1312
MwoI GCNNNNNNNGC 6 cut(s) 857, 1130, 1283, 1522, 1531, 1629
NciI CCSGG 1 cut(s) 125
NdeI CATATG 1 cut(s) 1530
NlaIII CATG 5 cut(s) 634, 1100, 1124, 1697, 1718
NlaIV GGNNCC 1 cut(s) 1575
NmuCI GTSAC 1 cut(s) 1495
NspI RCATGY 1 cut(s) 1124
PagI TCATGA 2 cut(s) 630, 1714
PceI AGGCCT 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1625
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 4 cut(s) 106, 905, 1081, 1790
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 2 cut(s) 1286, 1664
PfoI TCCNGGA 1 cut(s) 1310
PkrI GCNGC 5 cut(s) 862, 961, 1001, 1135, 1527
PleI GAGTC 1 cut(s) 1451
PpsI GAGTC 1 cut(s) 1451
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 1310
PspGI CCWGG 1 cut(s) 1310
PspN4I GGNNCC 1 cut(s) 1575
PspPI GGNCC 1 cut(s) 849
PstNI CAGNNNCTG 2 cut(s) 1286, 1547
PsuI RGATCY 2 cut(s) 835, 1325
PsyI GACNNNGTC 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 3 cut(s) 944, 1200, 1739
RsaNI GTAC 3 cut(s) 943, 1199, 1738
RseI CAYNNNNRTG 2 cut(s) 1252, 1719
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 2 cut(s) 819, 1173
SatI GCNGC 5 cut(s) 861, 960, 1000, 1134, 1526
Sau96I GGNCC 1 cut(s) 849
SchI GAGTC 1 cut(s) 1451
ScrFI CCNGG 2 cut(s) 125, 1312
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 3 cut(s) 1313, 1636, 1720
SmiMI CAYNNNNRTG 2 cut(s) 1252, 1719
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 9 cut(s) 21, 200, 246, 443, 689, 886, 1209, 1462, 1603
SseBI AGGCCT 1 cut(s) 372
SsiI CCGC 7 cut(s) 448, 915, 997, 1297, 1330, 1597, 1702
SspI AATATT 1 cut(s) 1780
SspMI CTAG 1 cut(s) 1512
SstI GAGCTC 1 cut(s) 735
StuI AGGCCT 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 123, 1310
StyI CCWWGG 4 cut(s) 222, 1017, 1333, 1511
TaaI ACNGT 4 cut(s) 485, 1267, 1543, 1629
TaiI ACGT 2 cut(s) 342, 1392
TaqI TCGA 4 cut(s) 138, 259, 462, 1729
TaqII GACCGA 1 cut(s) 944
TasI AATT 9 cut(s) 21, 200, 246, 443, 689, 886, 1209, 1462, 1603
TatI WGTACW 1 cut(s) 1198
TfiI GAWTC 4 cut(s) 106, 905, 1081, 1790
Tru1I TTAA 2 cut(s) 819, 1173
Tru9I TTAA 2 cut(s) 819, 1173
TscAI CASTG 4 cut(s) 490, 1270, 1548, 1634
TseFI GTSAC 1 cut(s) 1495
TseI GCWGC 5 cut(s) 860, 959, 999, 1133, 1525
Tsp45I GTSAC 1 cut(s) 1495
TspDTI ATGAA 8 cut(s) 375, 647, 654, 762, 1169, 1176, 1497, 1731
TspRI CASTG 4 cut(s) 490, 1270, 1548, 1634
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 2 cut(s) 1286, 1664
XapI RAATTY 5 cut(s) 21, 200, 886, 1462, 1603
XceI RCATGY 1 cut(s) 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1340
XmaJI CCTAGG 1 cut(s) 1511
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 1 cut(s) 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.