Rroxscaffold_5G00350310

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017424
Physical Location & Seq
Forward (+)
24101113 .. 24106568
5456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350310.1

Sequence Viewer

Length: 741 bp
ATGAAGAAAGCAAATTGGAATGGAAATCAACTTAGCTTGCGACGAGGAGGCCACTGCAAGTCTTTCGGCAAAATGCATTTGAAGAGAGGACGAGCTGCGGGATTGAGTCGCCTCTGCCATGAACTCCGGACTGTCCCTTCGCATCCGAACGAACAAGAAGATGGTGGTGGACTGGTTCAGCGAGTGGTGTCAACTACGGAGCCTGCGGTCTGCTCGAGTCCGGAGATAGCTGGTGGAGTTACGGTGGGTGCAGGTGCAGGCGTAGTGAGTGTTGGGGCCGGTGTGGCCACGAGCGATGGGGAGAAGATGAAGGGCGGTAGTGAAAGTTCGAGTCGGGTCTGCATCGAGAAGCTTGGATCGGCGGCAGCGGTACGGGGCGGCATCGAGAGTTCTGTCAATGAAGAATCCAAGGACTGCTTTGATCAAGATCTAAATCATGGTCGGCTTCGAGATGATATTTTCAATGCATTTAGTGACAGTTCGCTCGACACCACCACCCACGATGCATTTGACCAATTGCTGTTGCAATTCTTCTTGGAGACCTTGCAATGCAAGACGTACGTTTGTGAGTATGCGGACAAGGCACTAAGAGTAGTAGTGAGGTATTTGACTACTCTGCCTCTACTTCATAGGCTTAAAGGCTATGTCACCCAAGCCCACTTCAAAGAAGCGAGAAGAGGAATCGAGTCATGGAACAAGAAATCAAGGTCAAGCCTTCAAGGAAGTTACCTTCGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

246

Amino Acids

26.73

Weight (kDa)

8.96

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AccIII TCCGGA 2 cut(s) 126, 220
AciI CCGC 7 cut(s) 98, 206, 315, 362, 368, 378, 575
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 1 cut(s) 285
AfaI GTAC 2 cut(s) 372, 560
AgsI TTSAA 4 cut(s) 82, 463, 664, 719
AluBI AGCT 4 cut(s) 36, 95, 230, 352
AluI AGCT 4 cut(s) 36, 95, 230, 352
Alw26I GTCTC 1 cut(s) 533
AlwI GGATC 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 214
Aor13HI TCCGGA 2 cut(s) 126, 220
AoxI GGCC 3 cut(s) 49, 276, 285
ApeKI GCWGC 2 cut(s) 95, 365
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 640
AsuII TTCGAA 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 214
BalI TGGCCA 1 cut(s) 287
BauI CACGAG 1 cut(s) 289
BbvI GCAGC 2 cut(s) 82, 377
BccI CCATC 2 cut(s) 155, 290
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 427
BisI GCNGC 4 cut(s) 96, 363, 366, 379
BlsI GCNGC 4 cut(s) 97, 364, 367, 380
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 201, 277
BmsI GCATC 4 cut(s) 151, 351, 390, 493
Bpu14I TTCGAA 1 cut(s) 733
BsaBI GATNNNNATC 2 cut(s) 426, 432
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 2 cut(s) 126, 220
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 554
Bse8I GATNNNNATC 2 cut(s) 426, 432
BseAI TCCGGA 2 cut(s) 126, 220
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 142
BseJI GATNNNNATC 2 cut(s) 426, 432
BseMI GCAATG 1 cut(s) 554
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 82, 377
BsgI GTGCAG 2 cut(s) 270, 276
BshFI GGCC 3 cut(s) 51, 278, 287
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 3 cut(s) 127, 221, 279
BsiWI CGTACG 1 cut(s) 558
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 3 cut(s) 51, 278, 287
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 1 cut(s) 214
Bsp119I TTCGAA 1 cut(s) 733
Bsp13I TCCGGA 2 cut(s) 126, 220
Bsp143I GATC 3 cut(s) 356, 421, 427
BspACI CCGC 7 cut(s) 98, 206, 315, 362, 368, 378, 575
BspANI GGCC 3 cut(s) 51, 278, 287
BspEI TCCGGA 2 cut(s) 126, 220
BspLI GGNNCC 2 cut(s) 201, 277
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 733
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 3 cut(s) 356, 421, 427
BssSI CACGAG 1 cut(s) 289
BssT1I CCWWGG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 289
Bst4CI ACNGT 3 cut(s) 133, 244, 479
Bst6I CTCTTC 2 cut(s) 77, 670
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 3 cut(s) 38, 204, 259
BstDEI CTNAG 2 cut(s) 32, 587
BstF5I GGATG 1 cut(s) 142
BstKTI GATC 3 cut(s) 359, 424, 430
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 3 cut(s) 356, 421, 427
BstMWI GCNNNNNNNGC 2 cut(s) 284, 581
BstV1I GCAGC 2 cut(s) 82, 377
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 3 cut(s) 51, 278, 287
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 3 cut(s) 38, 204, 259
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 371, 559
CviAII CATG 3 cut(s) 119, 437, 690
CviQI GTAC 2 cut(s) 371, 559
DdeI CTNAG 2 cut(s) 32, 587
DpnI GATC 3 cut(s) 358, 423, 429
DpnII GATC 3 cut(s) 356, 421, 427
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 77, 670
EarI CTCTTC 2 cut(s) 77, 670
Eco130I CCWWGG 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 533
Eco88I CYCGRG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 408
EcoT22I ATGCAT 3 cut(s) 78, 469, 508
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 3 cut(s) 122, 440, 693
FaiI YATR 6 cut(s) 120, 438, 573, 630, 645, 691
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 118, 436, 689
FauI CCCGC 1 cut(s) 91
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 4 cut(s) 96, 363, 366, 379
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 4 cut(s) 96, 363, 366, 379
GluI GCNGC 4 cut(s) 96, 363, 366, 379
HaeIII GGCC 3 cut(s) 51, 278, 287
HapII CCGG 3 cut(s) 127, 221, 279
Hin1II CATG 3 cut(s) 122, 440, 693
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 6 cut(s) 106, 217, 331, 404, 681, 686
HpaII CCGG 3 cut(s) 127, 221, 279
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 2 cut(s) 170, 192
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 6 cut(s) 127, 221, 346, 385, 425, 449
Hpy8I GTNNAC 2 cut(s) 170, 192
Hpy99I CGWCG 1 cut(s) 45
HpyAV CCTTC 4 cut(s) 147, 304, 725, 740
HpyCH4III ACNGT 3 cut(s) 133, 244, 479
HpyCH4IV ACGT 2 cut(s) 557, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 581
HpyF3I CTNAG 2 cut(s) 32, 587
HpySE526I ACGT 2 cut(s) 557, 561
Hsp92II CATG 3 cut(s) 122, 440, 693
Kpn2I TCCGGA 2 cut(s) 126, 220
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 3 cut(s) 356, 421, 427
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 8 cut(s) 140, 158, 216, 216, 234, 237, 243, 292
Lsp1109I GCAGC 2 cut(s) 82, 377
LweI GCATC 4 cut(s) 151, 351, 390, 493
MaeII ACGT 2 cut(s) 557, 561
MaeIII GTNAC 4 cut(s) 238, 473, 646, 725
MalI GATC 3 cut(s) 358, 423, 429
MboI GATC 3 cut(s) 356, 421, 427
MboII GAAGA 7 cut(s) 16, 94, 170, 316, 413, 523, 687
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 4 cut(s) 13, 515, 527, 736
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 4 cut(s) 115, 226, 340, 695
MnlI CCTC 7 cut(s) 38, 41, 80, 122, 594, 630, 671
Mox20I TGGCCA 1 cut(s) 287
Mph1103I ATGCAT 3 cut(s) 78, 469, 508
MroI TCCGGA 2 cut(s) 126, 220
MscI TGGCCA 1 cut(s) 287
MseI TTAA 2 cut(s) 636, 739
Msp20I TGGCCA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 127, 221, 279
MunI CAATTG 1 cut(s) 515
MwoI GCNNNNNNNGC 2 cut(s) 284, 581
NdeII GATC 3 cut(s) 356, 421, 427
NlaIII CATG 3 cut(s) 122, 440, 693
NlaIV GGNNCC 2 cut(s) 201, 277
NmuCI GTSAC 2 cut(s) 473, 646
NsiI ATGCAT 3 cut(s) 78, 469, 508
NspV TTCGAA 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 214
PaqCI CACCTGC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 404, 681
Pfl23II CGTACG 1 cut(s) 558
PkrI GCNGC 4 cut(s) 97, 364, 367, 380
PleI GAGTC 4 cut(s) 114, 225, 339, 694
PpsI GAGTC 4 cut(s) 114, 225, 339, 694
PspLI CGTACG 1 cut(s) 558
PspN4I GGNNCC 2 cut(s) 201, 277
PspPI GGNCC 1 cut(s) 276
PspXI VCTCGAGB 1 cut(s) 214
PsrI GAACNNNNNNTAC 2 cut(s) 310, 342
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 372, 560
RsaNI GTAC 2 cut(s) 371, 559
SaqAI TTAA 2 cut(s) 636, 739
SatI GCNGC 4 cut(s) 96, 363, 366, 379
Sau3AI GATC 3 cut(s) 356, 421, 427
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 4 cut(s) 115, 226, 340, 695
SfaNI GCATC 4 cut(s) 151, 351, 390, 493
SfiI GGCCNNNNNGGCC 1 cut(s) 284
Sfr274I CTCGAG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 733
SlaI CTCGAG 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 4 cut(s) 13, 515, 527, 736
SsiI CCGC 7 cut(s) 98, 206, 315, 362, 368, 378, 575
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 3 cut(s) 133, 244, 479
TaiI ACGT 2 cut(s) 560, 564
TaqI TCGA 8 cut(s) 215, 329, 345, 384, 448, 486, 684, 733
TasI AATT 4 cut(s) 13, 515, 527, 736
TauI GCSGC 2 cut(s) 365, 381
TfiI GAWTC 2 cut(s) 404, 681
Tru1I TTAA 2 cut(s) 636, 739
Tru9I TTAA 2 cut(s) 636, 739
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 473, 646
TseI GCWGC 2 cut(s) 95, 365
Tsp45I GTSAC 2 cut(s) 473, 646
TspDTI ATGAA 5 cut(s) 17, 135, 323, 414, 617
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 1 cut(s) 59
XhoI CTCGAG 1 cut(s) 214
Zsp2I ATGCAT 3 cut(s) 78, 469, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.