Rorug03G0218300

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017424
Physical Location & Seq
Reverse (-)
19927145 .. 19928768
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0218300.1

Sequence Viewer

Length: 741 bp
ATGGCCGTCCCCGCAACCACATGGACGGCGATCTTCATCATCGCCGCCACGCTCCGTTTAATCGAATGCGGCGACAACAACCGCGTCTACTCGCCGTGCTCCGACACCAAGGTCGCCAGGTCCGACGGCTTCAGCTTCGGGATCGCCTTCGCCTCCAGGGACGTGTTTCTCCGAAACGGCGCGAACTCCACCTCCCAGTTGTCCCCGTGCGACACTAGACTCTCGCTCTCCACCGCCAATTCTCAGATCGCCGTTTTCCGGCCAAAGGTCGACGAGATCTCTCTCCTCTCCGTCAACTCCTCCTCTTTCGCTCCGGATAATTATGGGGGATATATGGTTGCTTTTGCTGGGCATAAATATGCTGCAAGGTCTACTGCGGCTTTTGTTGCCAATAGTACCTACACTGTCACCAGCTTTACTCTTGTGCTTGAGTTCACCAAGGGCAGGCTGCAGCACCTGTATTGGAAAAGGGATGGGTGCGCCAAATGTTCTGGGAGCTCCAGCTCCAGCTTCGTTTGCCTTAACAATCAGGACTGTGCAATCAAGACAAATAGCTGCAAAACCCATGGAGGCACTGTGGATTGCAGCCTCGGGATCCAGTTGGCATTTTCTGGGACGGATAAGCACCTATCGGTTCTGAACTCATGGTATGAAGTACAGAACCTTGGTCAGTACTCCCTTTATGGGCTCTATTCAAATCTGAAGGATTCTCTCACTAGCCAGTATAACAAGTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

246

Amino Acids

26.76

Weight (kDa)

8.7

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 3 cut(s) 87, 270, 371
AccII CGCG 2 cut(s) 84, 182
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 6 cut(s) 12, 45, 69, 82, 234, 377
AclWI GGATC 3 cut(s) 149, 589, 602
AcoI YGGCCR 2 cut(s) 3, 260
AcuI CTGAAG 2 cut(s) 115, 722
AfaI GTAC 3 cut(s) 397, 657, 674
AfiI CCNNNNNNNGG 4 cut(s) 258, 265, 444, 684
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 1 cut(s) 696
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 2 cut(s) 116, 155
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 6 cut(s) 135, 414, 498, 504, 510, 555
AluI AGCT 6 cut(s) 135, 414, 498, 504, 510, 555
Alw21I GWGCWC 2 cut(s) 101, 500
AlwI GGATC 3 cut(s) 149, 589, 602
AlwNI CAGNNNCTG 1 cut(s) 457
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 2 cut(s) 3, 260
ApeKI GCWGC 5 cut(s) 362, 448, 451, 555, 585
AspLEI GCGC 2 cut(s) 182, 482
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 400, 427
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 120
BamHI GGATCC 1 cut(s) 594
BanII GRGCYC 2 cut(s) 500, 690
Bbv12I GWGCWC 2 cut(s) 101, 500
BbvI GCAGC 5 cut(s) 349, 435, 463, 542, 597
BccI CCATC 1 cut(s) 467
BceAI ACGGC 5 cut(s) 42, 79, 142, 193, 236
BciT130I CCWGG 2 cut(s) 118, 157
BfaI CTAG 2 cut(s) 216, 717
BfmI CTRYAG 1 cut(s) 449
BglII AGATCT 1 cut(s) 276
BisI GCNGC 8 cut(s) 45, 70, 363, 378, 449, 452, 556, 586
BlsI GCNGC 8 cut(s) 46, 71, 364, 379, 450, 453, 557, 587
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 2 cut(s) 118, 157
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 596
BmrFI CCNGG 2 cut(s) 118, 157
BmrI ACTGGG 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 190
BpmI CTGGAG 3 cut(s) 139, 484, 490
BpuEI CTTGAG 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 35
BsaJI CCNNGG 6 cut(s) 108, 156, 438, 565, 589, 664
BsaWI WCCGGW 1 cut(s) 313
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 561, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 265, 444, 684
Bse1I ACTGG 3 cut(s) 196, 598, 721
Bse8I GATNNNNATC 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 2 cut(s) 118, 157
BseDI CCNNGG 6 cut(s) 108, 156, 438, 565, 589, 664
BseGI GGATG 1 cut(s) 478
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 4 cut(s) 258, 265, 444, 684
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 3 cut(s) 196, 598, 721
BseRI GAGGAG 3 cut(s) 275, 289, 292
BseXI GCAGC 5 cut(s) 349, 435, 463, 542, 597
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 2 cut(s) 84, 182
BshFI GGCC 2 cut(s) 5, 262
BsiHKAI GWGCWC 2 cut(s) 101, 500
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 2 cut(s) 259, 314
BslFI GGGAC 3 cut(s) 173, 187, 628
BslI CCNNNNNNNGG 4 cut(s) 258, 265, 444, 684
BsmFI GGGAC 3 cut(s) 173, 187, 628
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 2 cut(s) 5, 262
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 3 cut(s) 101, 500, 690
Bsp13I TCCGGA 1 cut(s) 313
Bsp143I GATC 5 cut(s) 30, 141, 246, 276, 594
Bsp19I CCATGG 1 cut(s) 565
BspACI CCGC 6 cut(s) 12, 45, 69, 82, 234, 377
BspANI GGCC 2 cut(s) 5, 262
BspCNI CTCAG 1 cut(s) 256
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 2 cut(s) 84, 182
BspLI GGNNCC 1 cut(s) 596
BspMAI CTGCAG 1 cut(s) 453
BspPI GGATC 3 cut(s) 149, 589, 602
BsrI ACTGG 3 cut(s) 196, 598, 721
BssECI CCNNGG 6 cut(s) 108, 156, 438, 565, 589, 664
BssMI GATC 5 cut(s) 30, 141, 246, 276, 594
BssT1I CCWWGG 4 cut(s) 108, 438, 565, 664
Bst2UI CCWGG 2 cut(s) 118, 157
Bst4CI ACNGT 3 cut(s) 406, 536, 577
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 2 cut(s) 84, 182
BstHHI GCGC 2 cut(s) 182, 482
BstKTI GATC 5 cut(s) 33, 144, 249, 279, 597
BstMBI GATC 5 cut(s) 30, 141, 246, 276, 594
BstMWI GCNNNNNNNGC 3 cut(s) 11, 386, 516
BstNI CCWGG 2 cut(s) 118, 157
BstSCI CCNGG 2 cut(s) 116, 155
BstSFI CTRYAG 1 cut(s) 449
BstUI CGCG 2 cut(s) 84, 182
BstV1I GCAGC 5 cut(s) 349, 435, 463, 542, 597
BstX2I RGATCY 2 cut(s) 276, 594
BstYI RGATCY 2 cut(s) 276, 594
BsuRI GGCC 2 cut(s) 5, 262
BtgI CCRYGG 1 cut(s) 565
BtgZI GCGATG 1 cut(s) 25
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 402, 573
Cac8I GCNNGC 1 cut(s) 446
CaiI CAGNNNCTG 1 cut(s) 457
CfoI GCGC 2 cut(s) 182, 482
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 396, 656, 673
CviAII CATG 3 cut(s) 21, 566, 645
CviQI GTAC 3 cut(s) 396, 656, 673
DdeI CTNAG 1 cut(s) 243
DpnI GATC 5 cut(s) 32, 143, 248, 278, 596
DpnII GATC 5 cut(s) 30, 141, 246, 276, 594
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 3, 260
Ecl136II GAGCTC 1 cut(s) 498
Eco130I CCWWGG 4 cut(s) 108, 438, 565, 664
Eco24I GRGCYC 2 cut(s) 500, 690
Eco47I GGWCC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 498
Eco57I CTGAAG 2 cut(s) 115, 722
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 116, 155
EcoT14I CCWWGG 4 cut(s) 108, 438, 565, 664
EcoT38I GRGCYC 2 cut(s) 500, 690
ErhI CCWWGG 4 cut(s) 108, 438, 565, 664
FaeI CATG 3 cut(s) 24, 569, 648
FaqI GGGAC 3 cut(s) 173, 187, 628
FatI CATG 3 cut(s) 20, 565, 644
FauI CCCGC 1 cut(s) 19
FblI GTMKAC 3 cut(s) 87, 270, 371
Fnu4HI GCNGC 8 cut(s) 45, 70, 363, 378, 449, 452, 556, 586
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 2 cut(s) 500, 690
Fsp4HI GCNGC 8 cut(s) 45, 70, 363, 378, 449, 452, 556, 586
FspBI CTAG 2 cut(s) 216, 717
GlaI GCGC 2 cut(s) 181, 481
GluI GCNGC 8 cut(s) 45, 70, 363, 378, 449, 452, 556, 586
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 3 cut(s) 139, 484, 490
HaeIII GGCC 2 cut(s) 5, 262
HapII CCGG 2 cut(s) 259, 314
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 182, 482
Hin1II CATG 3 cut(s) 24, 569, 648
Hin6I GCGC 2 cut(s) 180, 480
HinP1I GCGC 2 cut(s) 180, 480
HincII GTYRAC 2 cut(s) 271, 295
HindII GTYRAC 2 cut(s) 271, 295
HinfI GANTC 2 cut(s) 219, 707
HpaII CCGG 2 cut(s) 259, 314
HphI GGTGA 2 cut(s) 400, 427
Hpy166II GTNNAC 5 cut(s) 88, 271, 295, 372, 435
Hpy188I TCNGA 7 cut(s) 103, 124, 173, 246, 639, 702, 740
Hpy188III TCNNGA 5 cut(s) 139, 314, 530, 544, 592
Hpy8I GTNNAC 5 cut(s) 88, 271, 295, 372, 435
Hpy99I CGWCG 2 cut(s) 128, 275
HpyAV CCTTC 2 cut(s) 157, 697
HpyCH4III ACNGT 3 cut(s) 406, 536, 577
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 5 cut(s) 365, 451, 539, 558, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 386, 516
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 3 cut(s) 24, 569, 648
HspAI GCGC 2 cut(s) 180, 480
Kpn2I TCCGGA 1 cut(s) 313
Kzo9I GATC 5 cut(s) 30, 141, 246, 276, 594
LmnI GCTCC 6 cut(s) 57, 104, 316, 495, 503, 509
Lsp1109I GCAGC 5 cut(s) 349, 435, 463, 542, 597
MaeI CTAG 2 cut(s) 216, 717
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 406
MalI GATC 5 cut(s) 32, 143, 248, 278, 596
MboI GATC 5 cut(s) 30, 141, 246, 276, 594
MboII GAAGA 1 cut(s) 25
MflI RGATCY 2 cut(s) 276, 594
MhlI GDGCHC 3 cut(s) 101, 500, 690
MluCI AATT 2 cut(s) 238, 319
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 2 cut(s) 126, 147
MnlI CCTC 7 cut(s) 163, 202, 296, 310, 313, 563, 599
MroI TCCGGA 1 cut(s) 313
MseI TTAA 2 cut(s) 59, 522
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 2 cut(s) 259, 314
MspR9I CCNGG 2 cut(s) 118, 157
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 118, 157
MvnI CGCG 2 cut(s) 84, 182
MwoI GCNNNNNNNGC 3 cut(s) 11, 386, 516
NcoI CCATGG 1 cut(s) 565
NdeII GATC 5 cut(s) 30, 141, 246, 276, 594
NlaIII CATG 3 cut(s) 24, 569, 648
NlaIV GGNNCC 1 cut(s) 596
NmuCI GTSAC 1 cut(s) 406
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 120
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 707
PkrI GCNGC 8 cut(s) 46, 71, 364, 379, 450, 453, 557, 587
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 116, 155
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 2 cut(s) 116, 155
PspN4I GGNNCC 1 cut(s) 596
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 453
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 276, 594
RsaI GTAC 3 cut(s) 397, 657, 674
RsaNI GTAC 3 cut(s) 396, 656, 673
RseI CAYNNNNRTG 1 cut(s) 357
SacI GAGCTC 1 cut(s) 500
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 59, 522
SatI GCNGC 8 cut(s) 45, 70, 363, 378, 449, 452, 556, 586
Sau3AI GATC 5 cut(s) 30, 141, 246, 276, 594
Sau96I GGNCC 1 cut(s) 120
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 2 cut(s) 118, 157
SduI GDGCHC 3 cut(s) 101, 500, 690
SfcI CTRYAG 1 cut(s) 449
SinI GGWCC 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 2 cut(s) 238, 319
SsiI CCGC 6 cut(s) 12, 45, 69, 82, 234, 377
SspMI CTAG 2 cut(s) 216, 717
SstI GAGCTC 1 cut(s) 500
StyD4I CCNGG 2 cut(s) 116, 155
StyI CCWWGG 4 cut(s) 108, 438, 565, 664
TaaI ACNGT 3 cut(s) 406, 536, 577
TaiI ACGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 63, 270
TasI AATT 2 cut(s) 238, 319
TatI WGTACW 2 cut(s) 655, 672
TauI GCSGC 3 cut(s) 47, 72, 380
TfiI GAWTC 1 cut(s) 707
Tru1I TTAA 2 cut(s) 59, 522
Tru9I TTAA 2 cut(s) 59, 522
TscAI CASTG 2 cut(s) 409, 580
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 5 cut(s) 362, 448, 451, 555, 585
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 25, 666
TspGWI ACGGA 3 cut(s) 44, 280, 632
TspRI CASTG 2 cut(s) 409, 580
VpaK11BI GGWCC 1 cut(s) 120
XmiI GTMKAC 3 cut(s) 87, 270, 371
XspI CTAG 2 cut(s) 216, 717
ZrmI AGTACT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.