Rroxscaffold_5G00351820

Histidine protein methyltransferase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009281
Physical Location & Seq
Forward (+)
28477004 .. 28480764
3761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351820.1

Sequence Viewer

Length: 1053 bp
ATGCGCGCACCATCATTGCTTGCACAGTGCTTGCCGGGTTTGGTGCCTCATGACAAACCAAGTCACAGCGTGTCCTCTATTTCCGAGAGAGAATCACATCTCCCTTCACCGGCTGTTGAGATTCTCCCTTCCAAGTCAGCTCACCCATACAAGTATGCTGGTGAGAATATTGACTTGCAAGGACTCAATGCGTCAAAGGGAAGAGTTAGTGTTGCTGATATCATCGGCTTCAATGGTTCAGAAATGATATCTTCAAAACCAGATGGGTATCTAAAATCTTGGGATAGCTCTATTGATCTTGTTAATGTCCTGAAGCATGAGATTCGAGATGGGCAACTGAGCTTTAGAGGGAAAAGGGTACTTGAGCTGGGTTGCAGCTATGGCCTTCCAGGAATTTTTGCTTGTCTGAAGGGAGCTGGCACAGTGCACTTTCAAGACCTCAGTGCAGAAAGTATTAGATGCACAACAATACCAAATGTCCTTGCCAATCTTGAGCAAGCTCGAGATAGGCAGAGCCGACAGCCAGAGAGCCCCCTGACTCCATCAAGACAAACTGTGACCCAGTCGGTGCACTTTTATGCTGGTGACTGGGAAGAGCTTCCCGCTGTCTTGTCTGTTGTGAGGAATGATGGGTTTGAAGTGACAGCGGGAATGAACCTGAGCTTCTCTGAGGAAGATTTTATGGAGGGTTGTAGCAGCCAAGATGGAAGCATCATGGGACAAGAATCTTCCTCAAGGCGATCAAGGAAGCTTTCAGGAAGTCGGGCCTGGGAGAGAGCGAGTGAGACCGAACAAGGAGAGGGTGGATATGATATTATCTTGATGACTGAGATCCCATATTCATTAACCTCATTGAAAAAGCTTTATGCACTCATAAAAAAGTGTTCAAGGCCACCATATGGTGTTGTATACTTGGCCACAAAAAGGAATTATGTTGGCTTTAACGGCGGTGCTCGGCACCTGAAAAGCCTGGTAGATGAAGAGGGAATTTTTGGTGCTCATCTAGTGAAGGAGATGGCGGACAGAGATGTATGGAAATTTTTTAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

350

Amino Acids

38.51

Weight (kDa)

6.52

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 43, 957
AccB7I CCANNNNNTGG 1 cut(s) 899
AccI GTMKAC 1 cut(s) 909
AccII CGCG 1 cut(s) 6
AciI CCGC 4 cut(s) 603, 647, 948, 1019
AclWI GGATC 1 cut(s) 826
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 3 cut(s) 393, 987, 1037
AcuI CTGAAG 2 cut(s) 332, 428
AdeI CACNNNGTG 1 cut(s) 70
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 3 cut(s) 109, 899, 924
AgsI TTSAA 6 cut(s) 232, 255, 434, 638, 856, 888
AjnI CCWGG 3 cut(s) 388, 767, 969
Alw21I GWGCWC 4 cut(s) 429, 573, 955, 1000
Alw26I GTCTC 1 cut(s) 779
Alw44I GTGCAC 2 cut(s) 425, 569
AlwI GGATC 1 cut(s) 826
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 4 cut(s) 382, 765, 890, 915
ApaLI GTGCAC 2 cut(s) 425, 569
ApeKI GCWGC 2 cut(s) 375, 696
ApoI RAATTY 3 cut(s) 393, 987, 1037
Asp700I GAANNNNTTC 1 cut(s) 597
AspLEI GCGC 2 cut(s) 6, 8
AspS9I GGNCC 1 cut(s) 765
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 4 cut(s) 99, 134, 173, 596
AvaI CYCGRG 1 cut(s) 501
BaeGI GKGCMC 2 cut(s) 429, 573
BalI TGGCCA 1 cut(s) 917
BanI GGYRCC 2 cut(s) 43, 957
BanII GRGCYC 1 cut(s) 533
Bbv12I GWGCWC 4 cut(s) 429, 573, 955, 1000
BbvI GCAGC 2 cut(s) 387, 708
BccI CCATC 7 cut(s) 19, 257, 323, 550, 623, 698, 1009
BceAI ACGGC 1 cut(s) 961
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 3 cut(s) 390, 769, 971
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 1 cut(s) 1004
BisI GCNGC 2 cut(s) 376, 697
BlsI GCNGC 2 cut(s) 377, 698
Bme1390I CCNGG 4 cut(s) 36, 390, 769, 971
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 765
BmiI GGNNCC 2 cut(s) 45, 959
BmrFI CCNGG 4 cut(s) 36, 390, 769, 971
BmrI ACTGGG 2 cut(s) 556, 598
BmsI GCATC 2 cut(s) 449, 720
BmuI ACTGGG 2 cut(s) 556, 598
Bpu10I CCTNAGC 1 cut(s) 659
BpuEI CTTGAG 3 cut(s) 383, 512, 718
BpuMI CCSGG 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 768
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 899, 924
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 2 cut(s) 562, 593
Bse3DI GCAATG 1 cut(s) 14
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 3 cut(s) 390, 769, 971
BseDI CCNNGG 1 cut(s) 768
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 109, 899, 924
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 5 cut(s) 329, 454, 650, 660, 819
BseNI ACTGG 2 cut(s) 562, 593
BsePI GCGCGC 1 cut(s) 4
BseSI GKGCMC 2 cut(s) 429, 573
BseXI GCAGC 2 cut(s) 387, 708
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 6
BshFI GGCC 4 cut(s) 384, 767, 892, 917
BshNI GGYRCC 2 cut(s) 43, 957
BsiHKAI GWGCWC 4 cut(s) 429, 573, 955, 1000
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 2 cut(s) 35, 110
BslFI GGGAC 1 cut(s) 732
BslI CCNNNNNNNGG 3 cut(s) 109, 899, 924
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 732
BsnI GGCC 4 cut(s) 384, 767, 892, 917
Bso31I GGTCTC 1 cut(s) 779
BsoBI CYCGRG 1 cut(s) 501
Bsp1286I GDGCHC 5 cut(s) 429, 533, 573, 955, 1000
Bsp143I GATC 3 cut(s) 295, 740, 831
BspACI CCGC 4 cut(s) 603, 647, 948, 1019
BspANI GGCC 4 cut(s) 384, 767, 892, 917
BspCNI CTCAG 5 cut(s) 330, 453, 651, 661, 820
BspFNI CGCG 1 cut(s) 6
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 45, 959
BspPI GGATC 1 cut(s) 826
BspQI GCTCTTC 1 cut(s) 588
BspT107I GGYRCC 2 cut(s) 43, 957
BspTNI GGTCTC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 2 cut(s) 562, 593
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 768
BssHII GCGCGC 1 cut(s) 4
BssMI GATC 3 cut(s) 295, 740, 831
BssNAI GTATAC 1 cut(s) 910
Bst1107I GTATAC 1 cut(s) 910
Bst2UI CCWGG 3 cut(s) 390, 769, 971
Bst4CI ACNGT 3 cut(s) 27, 424, 556
Bst6I CTCTTC 3 cut(s) 196, 588, 975
BstC8I GCNNGC 5 cut(s) 6, 21, 32, 418, 498
BstDEI CTNAG 5 cut(s) 338, 440, 659, 669, 828
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 2 cut(s) 6, 8
BstKTI GATC 3 cut(s) 298, 743, 834
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 3 cut(s) 295, 740, 831
BstMWI GCNNNNNNNGC 2 cut(s) 381, 945
BstNI CCWGG 3 cut(s) 390, 769, 971
BstSCI CCNGG 4 cut(s) 34, 388, 767, 969
BstSLI GKGCMC 2 cut(s) 429, 573
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 387, 708
BstX2I RGATCY 1 cut(s) 831
BstYI RGATCY 1 cut(s) 831
BstZ17I GTATAC 1 cut(s) 910
BsuRI GGCC 4 cut(s) 384, 767, 892, 917
BtsIMutI CAGTG 3 cut(s) 32, 429, 448
Cac8I GCNNGC 5 cut(s) 6, 21, 32, 418, 498
CciI TCATGA 1 cut(s) 49
CfoI GCGC 2 cut(s) 6, 8
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 765
CseI GACGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 359
CviAII CATG 3 cut(s) 50, 317, 715
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 5 cut(s) 338, 440, 659, 669, 828
DpnI GATC 3 cut(s) 297, 742, 833
DpnII GATC 3 cut(s) 295, 740, 831
DraIII CACNNNGTG 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 3 cut(s) 196, 588, 975
EarI CTCTTC 3 cut(s) 196, 588, 975
EciI GGCGGA 1 cut(s) 1034
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 1 cut(s) 779
Eco32I GATATC 2 cut(s) 220, 249
Eco57I CTGAAG 2 cut(s) 332, 428
Eco88I CYCGRG 1 cut(s) 501
EcoRII CCWGG 3 cut(s) 388, 767, 969
EcoRV GATATC 2 cut(s) 220, 249
EcoT38I GRGCYC 1 cut(s) 533
FaeI CATG 3 cut(s) 53, 320, 718
FaqI GGGAC 1 cut(s) 732
FatI CATG 3 cut(s) 49, 316, 714
FauI CCCGC 2 cut(s) 610, 640
FauNDI CATATG 1 cut(s) 898
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 2 cut(s) 376, 697
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 2 cut(s) 376, 697
FspBI CTAG 1 cut(s) 1004
GlaI GCGC 2 cut(s) 5, 7
GluI GCNGC 2 cut(s) 376, 697
GsaI CCCAGC 1 cut(s) 371
HaeIII GGCC 4 cut(s) 384, 767, 892, 917
HapII CCGG 2 cut(s) 35, 110
HgaI GACGC 1 cut(s) 180
HhaI GCGC 2 cut(s) 6, 8
Hin1II CATG 3 cut(s) 53, 320, 718
Hin6I GCGC 2 cut(s) 4, 6
HinP1I GCGC 2 cut(s) 4, 6
HindIII AAGCTT 2 cut(s) 749, 860
HinfI GANTC 6 cut(s) 92, 121, 183, 322, 538, 725
HpaII CCGG 2 cut(s) 35, 110
HphI GGTGA 4 cut(s) 99, 134, 173, 596
Hpy166II GTNNAC 3 cut(s) 427, 571, 910
Hpy188I TCNGA 4 cut(s) 85, 241, 408, 670
Hpy188III TCNNGA 9 cut(s) 50, 310, 326, 434, 491, 503, 546, 756, 820
Hpy8I GTNNAC 3 cut(s) 427, 571, 910
HpyAV CCTTC 5 cut(s) 114, 138, 395, 403, 1003
HpyCH4III ACNGT 3 cut(s) 27, 424, 556
HpyCH4V TGCA 8 cut(s) 23, 178, 375, 427, 446, 462, 571, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 945
HpyF3I CTNAG 5 cut(s) 338, 440, 659, 669, 828
Hsp92II CATG 3 cut(s) 53, 320, 718
HspAI GCGC 2 cut(s) 4, 6
Kzo9I GATC 3 cut(s) 295, 740, 831
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 1 cut(s) 413
Lsp1109I GCAGC 2 cut(s) 387, 708
LweI GCATC 2 cut(s) 449, 720
MaeI CTAG 1 cut(s) 1004
MaeIII GTNAC 4 cut(s) 62, 556, 584, 640
MalI GATC 3 cut(s) 297, 742, 833
MboI GATC 3 cut(s) 295, 740, 831
MboII GAAGA 6 cut(s) 213, 243, 605, 686, 720, 992
MflI RGATCY 1 cut(s) 831
MhlI GDGCHC 5 cut(s) 429, 533, 573, 955, 1000
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 4 cut(s) 393, 928, 987, 1037
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 2 cut(s) 177, 532
Mox20I TGGCCA 1 cut(s) 917
MroXI GAANNNNTTC 1 cut(s) 597
MscI TGGCCA 1 cut(s) 917
MseI TTAA 4 cut(s) 303, 845, 942, 1044
MslI CAYNNNNRTG 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 2 cut(s) 605, 647
MspI CCGG 2 cut(s) 35, 110
MspR9I CCNGG 4 cut(s) 36, 390, 769, 971
MvaI CCWGG 3 cut(s) 390, 769, 971
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 381, 945
NciI CCSGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 898
NdeII GATC 3 cut(s) 295, 740, 831
NlaIII CATG 3 cut(s) 53, 320, 718
NlaIV GGNNCC 2 cut(s) 45, 959
NmeAIII GCCGAG 1 cut(s) 934
NmuCI GTSAC 4 cut(s) 62, 556, 584, 640
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 1 cut(s) 49
PauI GCGCGC 1 cut(s) 4
PciSI GCTCTTC 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 4 cut(s) 92, 121, 322, 725
PflFI GACNNNGTC 1 cut(s) 562
PflMI CCANNNNNTGG 1 cut(s) 899
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 2 cut(s) 377, 698
PleI GAGTC 2 cut(s) 177, 532
PpsI GAGTC 2 cut(s) 177, 532
Psp6I CCWGG 3 cut(s) 388, 767, 969
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 3 cut(s) 388, 767, 969
PspN4I GGNNCC 2 cut(s) 45, 959
PspPI GGNCC 1 cut(s) 765
PsuI RGATCY 1 cut(s) 831
PsyI GACNNNGTC 1 cut(s) 562
PteI GCGCGC 1 cut(s) 4
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 576
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 4 cut(s) 303, 845, 942, 1044
SatI GCNGC 2 cut(s) 376, 697
Sau3AI GATC 3 cut(s) 295, 740, 831
Sau96I GGNCC 1 cut(s) 765
SchI GAGTC 2 cut(s) 177, 532
ScrFI CCNGG 4 cut(s) 36, 390, 769, 971
SduI GDGCHC 5 cut(s) 429, 533, 573, 955, 1000
SfaNI GCATC 2 cut(s) 449, 720
Sfr274I CTCGAG 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 4 cut(s) 362, 491, 501, 733
SmoI CTYRAG 4 cut(s) 362, 491, 501, 733
Sse9I AATT 4 cut(s) 393, 928, 987, 1037
SsiI CCGC 4 cut(s) 603, 647, 948, 1019
SspI AATATT 1 cut(s) 169
SspMI CTAG 1 cut(s) 1004
StyD4I CCNGG 4 cut(s) 34, 388, 767, 969
TaaI ACNGT 3 cut(s) 27, 424, 556
TaqI TCGA 2 cut(s) 325, 502
TaqII GACCGA 1 cut(s) 803
TasI AATT 4 cut(s) 393, 928, 987, 1037
TfiI GAWTC 4 cut(s) 92, 121, 322, 725
Tru1I TTAA 4 cut(s) 303, 845, 942, 1044
Tru9I TTAA 4 cut(s) 303, 845, 942, 1044
TscAI CASTG 3 cut(s) 32, 429, 448
TseFI GTSAC 4 cut(s) 62, 556, 584, 640
TseI GCWGC 2 cut(s) 375, 696
Tsp45I GTSAC 4 cut(s) 62, 556, 584, 640
TspDTI ATGAA 3 cut(s) 668, 831, 993
TspRI CASTG 3 cut(s) 32, 429, 448
Tth111I GACNNNGTC 1 cut(s) 562
Van91I CCANNNNNTGG 1 cut(s) 899
VneI GTGCAC 2 cut(s) 425, 569
XapI RAATTY 3 cut(s) 393, 987, 1037
XhoI CTCGAG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 909
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 1 cut(s) 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.