Rroxscaffold_5G00364640

Condensin-2 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012287
Physical Location & Seq
Forward (+)
45854663 .. 45861079
6417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364640.1

Sequence Viewer

Length: 3972 bp
ATGGAAGAAACCATAACCCGAATAGTCACAGAGCTCGAAGACCTCCGCCACCTCGAAAACCACACCAACACCCAATCCCAACCCATCTCCGATTCCACTCTCTCCGACCTCCAAACCCTCTTCGACTCCAATGACCCCGACCCCATGGACCGCTTCTCCGACGAGCTCGCCGCCAAGTCCCTCTCCCTCTCCAATCTGGTCCGCCCCATCGCCGCCGCCATGGATTCCGGCCCGGCCCATTTCACCCTCTCAGCCTCCAAGCTCTACCTCTCTCTCCTCCTCTCCCCCAATTCCCCGGTCTGCACTCTCTTCACTCCCATGGCGTTCTTCTCTCTCCTCCAGTCGATTCGCCGGTCGCTGAAGCACCGCCGCGCCGAATCGGGTCACGGCGAGCCCGGCACGGGGTCCGGTAACGCGGCCGCTAGGAAGCGGAAGGGAAGAGGCAAAGGTAAGGGTTTGAAGAACAGTGAGGATTGCGAGAGCGAAGAGAGGGAGTTTGATGTTAGGGTTTTGTTTTCGGTGCTTGAAAAATTGGAACTTGTGATGGATTTGATTCATTTGGATCGGTTTCCGGATAGTTTGAAGTCGTTGGTTCAAACCGTGGCTGAAATTCCAGTCATGGGGTTTGAAGTTTGTGGCAATTCTGGTAATTATAACAGATTGACTAATTTGTGCTCTAGGGTTTTGCTTAAGGTGTTCAAGCCTGAGCATGGAGACCATGCCAGCATTGCAGCTGAGGTGTTCAAGTCATTGGCGCCGATGATTCTTCTGCATAAATCGCAGGTGAGGATATTTGCGTTGAGTTTTGTGGGGAATCAGATGATGGGTGTGGCTAAGAATCTTGAGGGGGTGAAGAAAGCAATGGTGAATTTTCTGTGGTACTTGGTCCACAAGGCGCCGGATAAGTCTGAGCCTCGGGCTTTGGCTGTCGAGTCTATAATGGAGATTGTTAGAGTTATGGAGGTTCAGGAGCAAGTTGTGTTTGTGGAGTATGTGGTGAAGATGACTCAAGGGAAGCCTAGGTTGAGGCTTTTGGCTGTTGACCTTATTCTGGTGCTGGTTACGTCTTTGAGAGATGTGATGGATTTGGATTCGGAGAGTGAAGTGAGGGATTCGTTGGGTTGGAAATGCTTGGAGGGTTTGATTCAGCGCTGTTCGGATGCAATGGCTGGGATTCGAGCTCGTGCTTTGTCGAATTTGTCTCAGCTGGTTGGCTTCTTGTCTGGTGATGATAGGAGCCGGGAAGTGTTGAAAGAAGTTATGGGGTTTGGTGATGCAATGGATGAAAGGCAGGAGGGCTGGATGAATGAGATTTTGAGGGCAAGGTGTATGGATGAGAAGGCAGCTGTAAGGAAGGCTGCACTTCTTTTGGTTACCAAGTTGATGGCCATTCTTGGTAGTGGCTTTGATAGAGCCTTGATCAAGACTATGGGCATGGCTTGTTCTGATCCACTTGTTAGCATACGCAAAGCGGCAATTTCAGCTCTTTCAGTGGCTTTTAGAACATTCCTGGATGATAGCGTGGCTACTGAGTGGCTACATTCTGTTCCAGCTTTAATAACTGATAATGAGTCTAGCATTCAAGAAGAATGTGAAAACTTGTTTGTAGAGCTGGTATTGGAAAGGGTATCTACAGTTGGTTCTGAAATCAATACTGACTCAGTACTTCCTGAAGGCATTTTAAGCCTTTTGAAAGAGATTTGCAATGGAGAAGTGGCACCTTGGGTGAAGAAAATTTTAACAAACTTGGGAAAGAAGAATCGACTGAAGCACAAATTTGCTACTGCACTTCAGAGTATCATAAGGACATCTGAGTCTCTCTGGCTCAGTCAATCCATGCCTATAGAGAAATGGACAGCCCCACCAGGCTCTTGGTTTCTTCTGTCAGAGGTGTCAGCATACCTTGCAAAAGCAGTTGACTGGGAGTTTCTCCGTCATCATTGGCAGCTTTTTGACAAGCATGGAGTGGGAGGGGAGATTCAGAGTCCACTTCCACAGGGATATGCACATGAAGAGGGAGGGGGTATAGAATCCAGTTCTGTTGCTTGGGCTGGGGATCGTGTTTTTCTCTTACAGACTATTTCTAATGTATCTGTTGAGCTGCCTTCTGAAGTTGGTGCAGAGTTAGCTCATGACTTGCTTAAAAGAATTGAAAATTTTAACATGCATTCAACAGAGGTTAATGCTCATGTAAAAGCACTCAGAACATTGTGCAAGCGGAAGGTTTCAGATCCAGAGCAGGCTGATACACTTGTTAAAAGATGCGTACATCAGATTGCCTCAAAAGCTTCCAAGATATTAGATAAGTATATATCCAAGGAGTCAAATACAACCCGAAAAGGTGGCTTCTTTACACCACCTATAAGTGGGAGTAGAATGGGCAAGAGGGCCATAACCATGTCCAAGTCTTTGTCAAAAGTAGTGACAGCAGTCTATACTATTGGGTCTTTGGTTATTATTTGTCCATCTGCTGATATGAGCACCACCATTCCACTACTATATACCATCATCACTTCTGGGAATTCTGATCCAAAAGTCAATAAACTAGAAGGCTCTATAGGATCTCTAAAGCAGACAGCACCTTCTTTGTACATTCAAGCATGGTTAACTATGGGGAAGATTTGCCTTACAGATGAAAAAATTGCAAAGACGTATATTCCACTCTTTGTGCAGGAACTTGAAAAGAGTGATTGTGCAGCCCTTCGCAACAATCTGGTAGTTGTGATGGCAGATTTTTGCATTCGACATACTTCTTTAGTTGATAGTTATATACCAAGGATCACAAAATGCCTCCGTGATCCATGCGAACTTGTAAGAAGGCAGACATTTATACTGCTCTCAAGATTGTTGCAGAGGGATTATGTGAAGTGGAGAGGAGTTCTTTTCCTGAGATTTCTCTTGTCACTTGTTGATGAGTCAGAAAAGATAAAGCAGCTCGCCACCTTTCTTTTCAGCAATATCTTGAAAGCTAAGGCGCCTCTTCTCGGATATAACAGTTTTGTAGAAGCAATTTTTGTTCTGAATGACTGCCGTGCCCACAAGGGACATACTGGTGCTCAGGGTTCACGAGCAGAGAGCCGCCTTTTTTCCATTAGGGGAAATGATGAAGCTTCGAGGTCTAGAAGAATGCACATCTATGTTTCTTTGCTGAAACAAATGGCTCCAGAGCACTTACTGCAAACGTTTGTAAAGTTATGTACAGAGATCCTTGCTGCAGCATCTGATGGCATGCTCAATATAGATGATATTACTGGGCAGGCTGTTCTGCAGGATGCTTTCCAAATTATTGCCTGCAAAGAAATACGAATACCATCCAACCGTGGGTTATCATCTGACACTGGAGATATTGATGAAGAAGGTGGTGATAATGGTGGAGCAGCTGCTAAGGGAAGGGCAATAACTCAAGCACTCAAAAAGGGCCTCATCCAAAGCACAATACCCATCTTTATAGAGCTGAAGCGGCTATTGGAAAGCAAAAACAGCCCCCTTGTAGGCTCCCTCATGGAATGCCTCCGAATCATCCTCAAGGATTACAAAAATGAGATCGAAGACATATTAGTTGCTGATAAGCAGCTCCAGAAAGAGCTGATATATGACATGCAGAAGTATGAAAAGGCAAAGGTCAAGTCTACAGCTGCCGAGACTGTTGCTAACCATCAAAGAACAGTTAGCTTCAACTCTCCCGGCATATCCAAGATTGCAAGCGTGAGACAGGCGCATAACAAATCCATTGGCAGATTGCAAGGCGACGTGAGATTAGCTTCGGCAATGGGAGATGTAGCTGCTGAAGCCATAGCAAGGTCAGTGCTCAAGGACATGAACAACGGGAGACAGACACCACCTCTCAGCTCTTTAAGCGTGCCTAAGCTCAAGACCTGCGAGGGAGGGCAGAGCTCTCGAAGTGGTGTGCCCTTGGATGTATTGGAATCTGTAAGAAGAAGACAGAATTTTGACTTTGTTGAGGAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cnd1

Protein Analysis

1323

Amino Acids

146.46

Weight (kDa)

8.4

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cnd1 PF12717 901 - 1075 1e-18 non-SMC mitotic condensation complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15890 AT4G15890
fragaria_vesca FvH4_4g18310
malus_domestica MD16G1167900.v1.1
prunus_persica Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1
pyrus_communis pycom16g14150
rosa_chinensis RchiOBHm_Chr4g0422791
rosa_laevigata RLG00000007605
rosa_multiflora Rmu_sc0000830.1_g000049
rosa_roxburghii Rroxscaffold_5G00364640
rosa_rugosa Rorug04G0181400
rosa_samantha Rh4AG241800 Rh4BG245200 Rh4CG255700 Rh4DG240400
rosa_wichuraiana Rw4G020810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AarI CACCTGC 1 cut(s) 772
AasI GACNNNNNNGTC 1 cut(s) 1813
Acc36I ACCTGC 2 cut(s) 772, 3884
AccB1I GGYRCC 4 cut(s) 754, 895, 1717, 2974
AccI GTMKAC 1 cut(s) 3629
AccII CGCG 2 cut(s) 372, 416
AccIII TCCGGA 1 cut(s) 571
AclI AACGTT 1 cut(s) 3182
AclWI GGATC 9 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792, 3199
AcoI YGGCCR 2 cut(s) 417, 1386
AcsI RAATTY 8 cut(s) 609, 868, 1195, 1734, 1775, 2155, 2521, 3946
AcuI CTGAAG 7 cut(s) 380, 1692, 1775, 1787, 2130, 3476, 3807
AcyI GRCGYC 3 cut(s) 755, 896, 2975
AfaI GTAC 5 cut(s) 881, 1665, 2268, 2591, 3199
AfeI AGCGCT 1 cut(s) 1151
AflII CTTAAG 1 cut(s) 689
AjiI CACGTC 1 cut(s) 3751
AjnI CCWGG 2 cut(s) 1509, 1864
AjuI GAANNNNNNNTTGG 2 cut(s) 3449, 3481
Alw21I GWGCWC 9 cut(s) 36, 168, 677, 1183, 2483, 3058, 3171, 3810, 3896
Alw26I GTCTC 6 cut(s) 708, 1206, 1821, 3635, 3703, 3823
AlwI GGATC 9 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792, 3199
AlwNI CAGNNNCTG 1 cut(s) 3221
Ama87I CYCGRG 1 cut(s) 915
Aor13HI TCCGGA 1 cut(s) 571
Aor51HI AGCGCT 1 cut(s) 1151
AoxI GGCC 6 cut(s) 229, 234, 417, 1386, 2388, 3418
ApoI RAATTY 8 cut(s) 609, 868, 1195, 1734, 1775, 2155, 2521, 3946
ArsI GACNNNNNNTTYG 2 cut(s) 3611, 3643
AspA2I CCTAGG 1 cut(s) 1019
AspLEI GCGC 6 cut(s) 374, 757, 898, 1152, 2977, 3718
AspS9I GGNCC 8 cut(s) 148, 199, 230, 235, 405, 886, 2388, 3418
AsuC2I CCSGG 5 cut(s) 233, 296, 396, 1241, 3684
AsuHPI GGTGA 9 cut(s) 235, 796, 862, 877, 1009, 1238, 1283, 1738, 3374
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 4 cut(s) 148, 199, 405, 886
AvrII CCTAGG 1 cut(s) 1019
BaeGI GKGCMC 2 cut(s) 3037, 3912
BaeI ACNNNNGTAYC 2 cut(s) 2238, 2271
BalI TGGCCA 1 cut(s) 1388
BanI GGYRCC 4 cut(s) 754, 895, 1717, 2974
BanII GRGCYC 5 cut(s) 36, 168, 396, 1183, 3896
BauI CACGAG 2 cut(s) 1182, 3066
BbsI GAAGAC 3 cut(s) 45, 3555, 3946
Bbv12I GWGCWC 9 cut(s) 36, 168, 677, 1183, 2483, 3058, 3171, 3810, 3896
BbvCI CCTCAGC 1 cut(s) 735
BceAI ACGGC 2 cut(s) 403, 3015
BcgI CGANNNNNNTGC 4 cut(s) 3629, 3663, 3878, 3912
BciT130I CCWGG 2 cut(s) 1511, 1866
BclI TGATCA 1 cut(s) 1419
BcnI CCSGG 5 cut(s) 233, 296, 396, 1241, 3684
BcoDI GTCTC 6 cut(s) 708, 1206, 1821, 3635, 3703, 3823
BfaI CTAG 6 cut(s) 423, 678, 1020, 1575, 2546, 3120
BfmI CTRYAG 6 cut(s) 1632, 1842, 2556, 3213, 3266, 3630
BfoI RGCGCY 4 cut(s) 758, 899, 1153, 2978
BfrI CTTAAG 1 cut(s) 689
BfuAI ACCTGC 2 cut(s) 772, 3884
BlnI CCTAGG 1 cut(s) 1019
BmcAI AGTACT 1 cut(s) 1665
Bme1390I CCNGG 7 cut(s) 233, 296, 396, 1241, 1511, 1866, 3684
Bme18I GGWCC 4 cut(s) 148, 199, 405, 886
BmeT110I CYCGRG 1 cut(s) 915
BmgBI CACGTC 1 cut(s) 3751
BmgT120I GGNCC 8 cut(s) 148, 199, 230, 235, 405, 886, 2388, 3418
BmiI GGNNCC 8 cut(s) 406, 756, 897, 1238, 1719, 2976, 3162, 3496
BmrFI CCNGG 7 cut(s) 233, 296, 396, 1241, 1511, 1866, 3684
BmrI ACTGGG 2 cut(s) 1930, 3261
BmsI GCATC 5 cut(s) 1150, 1264, 2252, 3227, 3262
BmuI ACTGGG 2 cut(s) 1930, 3261
BoxI GACNNNNGTC 1 cut(s) 2429
BpiI GAAGAC 3 cut(s) 45, 3555, 3946
BpmI CTGGAG 4 cut(s) 323, 3147, 3360, 3560
Bpu10I CCTNAGC 6 cut(s) 705, 735, 2970, 3057, 3384, 3864
BpuEI CTTGAG 7 cut(s) 863, 993, 2824, 3387, 3509, 3794, 3854
BpuMI CCSGG 5 cut(s) 233, 296, 396, 1241, 3684
BsaHI GRCGYC 3 cut(s) 755, 896, 2975
BsaI GGTCTC 1 cut(s) 708
BsaWI WCCGGW 2 cut(s) 407, 571
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 7 cut(s) 340, 614, 1925, 2034, 3055, 3256, 3343
Bse3DI GCAATG 6 cut(s) 726, 867, 1170, 1284, 1711, 3774
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 2 cut(s) 1511, 1866
BseMI GCAATG 6 cut(s) 726, 867, 1170, 1284, 1711, 3774
BseNI ACTGG 7 cut(s) 340, 614, 1925, 2034, 3055, 3256, 3343
BseRI GAGGAG 4 cut(s) 266, 269, 326, 2889
BseSI GKGCMC 2 cut(s) 3037, 3912
BseX3I CGGCCG 1 cut(s) 417
BseYI CCCAGC 2 cut(s) 1169, 2051
BsgI GTGCAG 6 cut(s) 286, 1344, 1770, 2139, 2690, 2715
Bsh1236I CGCG 2 cut(s) 372, 416
Bsh1285I CGRYCG 2 cut(s) 356, 420
BshFI GGCC 6 cut(s) 231, 236, 419, 1388, 2390, 3420
BshNI GGYRCC 4 cut(s) 754, 895, 1717, 2974
BsiEI CGRYCG 2 cut(s) 356, 420
BsiHKAI GWGCWC 9 cut(s) 36, 168, 677, 1183, 2483, 3058, 3171, 3810, 3896
BsiHKCI CYCGRG 1 cut(s) 915
BslFI GGGAC 2 cut(s) 163, 3057
BsmAI GTCTC 6 cut(s) 708, 1206, 1821, 3635, 3703, 3823
BsmFI GGGAC 2 cut(s) 163, 3057
BsmI GAATGC 5 cut(s) 1578, 2167, 2739, 3132, 3512
BsnI GGCC 6 cut(s) 231, 236, 419, 1388, 2390, 3420
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 1 cut(s) 915
Bsp13I TCCGGA 1 cut(s) 571
Bsp1407I TGTACA 2 cut(s) 2589, 3197
Bsp19I CCATGG 3 cut(s) 144, 219, 318
BspANI GGCC 6 cut(s) 231, 236, 419, 1388, 2390, 3420
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 2 cut(s) 372, 416
BspHI TCATGA 1 cut(s) 2131
BspLI GGNNCC 8 cut(s) 406, 756, 897, 1238, 1719, 2976, 3162, 3496
BspMAI CTGCAG 2 cut(s) 3217, 3270
BspMI ACCTGC 2 cut(s) 772, 3884
BspPI GGATC 9 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792, 3199
BspT107I GGYRCC 4 cut(s) 754, 895, 1717, 2974
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 6 cut(s) 726, 867, 1170, 1284, 1711, 3774
BsrFI RCCGGY 1 cut(s) 351
BsrGI TGTACA 2 cut(s) 2589, 3197
BsrI ACTGG 7 cut(s) 340, 614, 1925, 2034, 3055, 3256, 3343
BssAI RCCGGY 1 cut(s) 351
BssNI GRCGYC 3 cut(s) 755, 896, 2975
BssSI CACGAG 2 cut(s) 1182, 3066
BssT1I CCWWGG 8 cut(s) 144, 219, 318, 1019, 1721, 2316, 2774, 3912
Bst2BI CACGAG 2 cut(s) 1182, 3066
Bst2UI CCWGG 2 cut(s) 1511, 1866
Bst4CI ACNGT 7 cut(s) 467, 601, 1636, 2996, 3320, 3646, 3667
Bst6I CTCTTC 6 cut(s) 125, 314, 433, 480, 2007, 2985
BstACI GRCGYC 3 cut(s) 755, 896, 2975
BstAFI CTTAAG 1 cut(s) 689
BstAPI GCANNNNNTGC 2 cut(s) 1904, 3175
BstAUI TGTACA 2 cut(s) 2589, 3197
BstDSI CCRYGG 5 cut(s) 144, 219, 318, 600, 3319
BstEII GGTNACC 1 cut(s) 1372
BstFNI CGCG 2 cut(s) 372, 416
BstH2I RGCGCY 4 cut(s) 758, 899, 1153, 2978
BstHHI GCGC 6 cut(s) 374, 757, 898, 1152, 2977, 3718
BstMAI GTCTC 6 cut(s) 708, 1206, 1821, 3635, 3703, 3823
BstMCI CGRYCG 2 cut(s) 356, 420
BstNI CCWGG 2 cut(s) 1511, 1866
BstNSI RCATGY 3 cut(s) 2167, 3232, 3601
BstPAI GACNNNNGTC 1 cut(s) 2429
BstPI GGTNACC 1 cut(s) 1372
BstSCI CCNGG 7 cut(s) 231, 294, 394, 1239, 1509, 1864, 3682
BstSFI CTRYAG 6 cut(s) 1632, 1842, 2556, 3213, 3266, 3630
BstSLI GKGCMC 2 cut(s) 3037, 3912
BstUI CGCG 2 cut(s) 372, 416
BstV2I GAAGAC 3 cut(s) 45, 3555, 3946
BstX2I RGATCY 3 cut(s) 2230, 2561, 3204
BstXI CCANNNNNNTGG 2 cut(s) 1384, 1872
BstYI RGATCY 3 cut(s) 2230, 2561, 3204
BstZI CGGCCG 1 cut(s) 417
BsuRI GGCC 6 cut(s) 231, 236, 419, 1388, 2390, 3420
BtgI CCRYGG 5 cut(s) 144, 219, 318, 600, 3319
BtgZI GCGATG 1 cut(s) 193
BtrI CACGTC 1 cut(s) 3751
BtsIMutI CAGTG 4 cut(s) 472, 1497, 3336, 3810
BveI ACCTGC 2 cut(s) 772, 3884
CaiI CAGNNNCTG 1 cut(s) 3221
CciI TCATGA 1 cut(s) 2131
CciNI GCGGCCGC 1 cut(s) 417
CfoI GCGC 6 cut(s) 374, 757, 898, 1152, 2977, 3718
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 8 cut(s) 148, 199, 230, 235, 405, 886, 2388, 3418
Csp6I GTAC 5 cut(s) 880, 1664, 2267, 2590, 3198
CviQI GTAC 5 cut(s) 880, 1664, 2267, 2590, 3198
DinI GGCGCC 3 cut(s) 756, 897, 2976
DrdI GACNNNNNNGTC 1 cut(s) 1813
DseDI GACNNNNNNGTC 1 cut(s) 1813
EaeI YGGCCR 2 cut(s) 417, 1386
EagI CGGCCG 1 cut(s) 417
Eam1104I CTCTTC 6 cut(s) 125, 314, 433, 480, 2007, 2985
EarI CTCTTC 6 cut(s) 125, 314, 433, 480, 2007, 2985
EciI GGCGGA 2 cut(s) 35, 191
Ecl136II GAGCTC 4 cut(s) 34, 166, 1181, 3894
EclXI CGGCCG 1 cut(s) 417
Eco130I CCWWGG 8 cut(s) 144, 219, 318, 1019, 1721, 2316, 2774, 3912
Eco24I GRGCYC 5 cut(s) 36, 168, 396, 1183, 3896
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 4 cut(s) 148, 199, 405, 886
Eco47III AGCGCT 1 cut(s) 1151
Eco52I CGGCCG 1 cut(s) 417
Eco53kI GAGCTC 4 cut(s) 34, 166, 1181, 3894
Eco57I CTGAAG 7 cut(s) 380, 1692, 1775, 1787, 2130, 3476, 3807
Eco88I CYCGRG 1 cut(s) 915
Eco91I GGTNACC 1 cut(s) 1372
EcoICRI GAGCTC 4 cut(s) 34, 166, 1181, 3894
EcoO109I RGGNCCY 1 cut(s) 3418
EcoO65I GGTNACC 1 cut(s) 1372
EcoRI GAATTC 1 cut(s) 2521
EcoRII CCWGG 2 cut(s) 1509, 1864
EcoT14I CCWWGG 8 cut(s) 144, 219, 318, 1019, 1721, 2316, 2774, 3912
EcoT22I ATGCAT 1 cut(s) 2169
EcoT38I GRGCYC 5 cut(s) 36, 168, 396, 1183, 3896
EgeI GGCGCC 3 cut(s) 756, 897, 2976
EheI GGCGCC 3 cut(s) 756, 897, 2976
ErhI CCWWGG 8 cut(s) 144, 219, 318, 1019, 1721, 2316, 2774, 3912
FaqI GGGAC 2 cut(s) 163, 3057
FbaI TGATCA 1 cut(s) 1419
FblI GTMKAC 1 cut(s) 3629
FriOI GRGCYC 5 cut(s) 36, 168, 396, 1183, 3896
FspBI CTAG 6 cut(s) 423, 678, 1020, 1575, 2546, 3120
GlaI GCGC 6 cut(s) 373, 756, 897, 1151, 2976, 3717
GsaI CCCAGC 2 cut(s) 1173, 2055
GsuI CTGGAG 4 cut(s) 323, 3147, 3360, 3560
HaeII RGCGCY 4 cut(s) 758, 899, 1153, 2978
HaeIII GGCC 6 cut(s) 231, 236, 419, 1388, 2390, 3420
HhaI GCGC 6 cut(s) 374, 757, 898, 1152, 2977, 3718
Hin1I GRCGYC 3 cut(s) 755, 896, 2975
Hin6I GCGC 6 cut(s) 372, 755, 896, 1150, 2975, 3716
HinP1I GCGC 6 cut(s) 372, 755, 896, 1150, 2975, 3716
HincII GTYRAC 3 cut(s) 1042, 1918, 2607
HindII GTYRAC 3 cut(s) 1042, 1918, 2607
HindIII AAGCTT 2 cut(s) 2286, 3108
HpaI GTTAAC 1 cut(s) 2607
HphI GGTGA 9 cut(s) 235, 796, 862, 877, 1009, 1238, 1283, 1738, 3374
Hpy166II GTNNAC 7 cut(s) 889, 1042, 1918, 1988, 2607, 3065, 3630
Hpy8I GTNNAC 7 cut(s) 889, 1042, 1918, 1988, 2607, 3065, 3630
Hpy99I CGWCG 2 cut(s) 164, 3752
HpyCH4III ACNGT 7 cut(s) 467, 601, 1636, 2996, 3320, 3646, 3667
HpyCH4IV ACGT 4 cut(s) 1064, 2651, 3182, 3750
HpySE526I ACGT 4 cut(s) 1064, 2651, 3182, 3750
Hsp92I GRCGYC 3 cut(s) 755, 896, 2975
HspAI GCGC 6 cut(s) 372, 755, 896, 1150, 2975, 3716
KasI GGCGCC 3 cut(s) 754, 895, 2974
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1419
KspAI GTTAAC 1 cut(s) 2607
LmnI GCTCC 6 cut(s) 970, 1236, 3166, 3374, 3500, 3579
LweI GCATC 5 cut(s) 1150, 1264, 2252, 3227, 3262
MaeI CTAG 6 cut(s) 423, 678, 1020, 1575, 2546, 3120
MaeII ACGT 4 cut(s) 1064, 2651, 3182, 3750
MaeIII GTNAC 7 cut(s) 25, 383, 410, 1060, 1372, 2422, 2901
MflI RGATCY 3 cut(s) 2230, 2561, 3204
MlsI TGGCCA 1 cut(s) 1388
MluNI TGGCCA 1 cut(s) 1388
Mly113I GGCGCC 3 cut(s) 755, 896, 2975
MlyI GAGTC 9 cut(s) 119, 941, 1000, 1580, 1652, 1823, 1993, 2330, 2924
MmeI TCCRAC 4 cut(s) 129, 183, 1103, 3339
Mox20I TGGCCA 1 cut(s) 1388
Mph1103I ATGCAT 1 cut(s) 2169
MroI TCCGGA 1 cut(s) 571
MscI TGGCCA 1 cut(s) 1388
MslI CAYNNNNRTG 4 cut(s) 317, 2396, 3051, 3135
Msp20I TGGCCA 1 cut(s) 1388
MspA1I CMGCKG 5 cut(s) 734, 1207, 1346, 3380, 3635
MspCI CTTAAG 1 cut(s) 689
MspR9I CCNGG 7 cut(s) 233, 296, 396, 1241, 1511, 1866, 3684
Mva1269I GAATGC 5 cut(s) 1578, 2167, 2739, 3132, 3512
MvaI CCWGG 2 cut(s) 1511, 1866
MvnI CGCG 2 cut(s) 372, 416
NarI GGCGCC 3 cut(s) 755, 896, 2975
NciI CCSGG 5 cut(s) 233, 296, 396, 1241, 3684
NcoI CCATGG 3 cut(s) 144, 219, 318
NlaIV GGNNCC 8 cut(s) 406, 756, 897, 1238, 1719, 2976, 3162, 3496
NmeAIII GCCGAG 1 cut(s) 3664
NmuCI GTSAC 4 cut(s) 25, 383, 2422, 2901
NotI GCGGCCGC 1 cut(s) 417
NsiI ATGCAT 1 cut(s) 2169
NspI RCATGY 3 cut(s) 2167, 3232, 3601
PaeI GCATGC 1 cut(s) 3232
PagI TCATGA 1 cut(s) 2131
PaqCI CACCTGC 1 cut(s) 772
PctI GAATGC 5 cut(s) 1578, 2167, 2739, 3132, 3512
PfoI TCCNGGA 1 cut(s) 1509
PleI GAGTC 9 cut(s) 119, 940, 1000, 1579, 1652, 1822, 1992, 2329, 2923
PluTI GGCGCC 3 cut(s) 758, 899, 2978
PpsI GAGTC 9 cut(s) 119, 940, 1000, 1579, 1652, 1822, 1992, 2329, 2923
PshAI GACNNNNGTC 1 cut(s) 2429
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 4 cut(s) 36, 168, 1183, 3896
Psp1406I AACGTT 1 cut(s) 3182
Psp6I CCWGG 2 cut(s) 1509, 1864
PspEI GGTNACC 1 cut(s) 1372
PspFI CCCAGC 2 cut(s) 1169, 2051
PspGI CCWGG 2 cut(s) 1509, 1864
PspN4I GGNNCC 8 cut(s) 406, 756, 897, 1238, 1719, 2976, 3162, 3496
PspPI GGNCC 8 cut(s) 148, 199, 230, 235, 405, 886, 2388, 3418
PstI CTGCAG 2 cut(s) 3217, 3270
PstNI CAGNNNCTG 1 cut(s) 3221
PsuI RGATCY 3 cut(s) 2230, 2561, 3204
PvuII CAGCTG 5 cut(s) 734, 1207, 1346, 3380, 3635
RsaI GTAC 5 cut(s) 881, 1665, 2268, 2591, 3199
RsaNI GTAC 5 cut(s) 880, 1664, 2267, 2590, 3198
RseI CAYNNNNRTG 4 cut(s) 317, 2396, 3051, 3135
SacI GAGCTC 4 cut(s) 36, 168, 1183, 3896
Sau96I GGNCC 8 cut(s) 148, 199, 230, 235, 405, 886, 2388, 3418
ScaI AGTACT 1 cut(s) 1665
SchI GAGTC 9 cut(s) 119, 941, 1000, 1580, 1652, 1823, 1993, 2330, 2924
ScrFI CCNGG 7 cut(s) 233, 296, 396, 1241, 1511, 1866, 3684
SfaNI GCATC 5 cut(s) 1150, 1264, 2252, 3227, 3262
SfcI CTRYAG 6 cut(s) 1632, 1842, 2556, 3213, 3266, 3630
SfoI GGCGCC 3 cut(s) 756, 897, 2976
SinI GGWCC 4 cut(s) 148, 199, 405, 886
SmiMI CAYNNNNRTG 4 cut(s) 317, 2396, 3051, 3135
SmlI CTYRAG 8 cut(s) 689, 842, 1008, 2839, 3402, 3524, 3809, 3869
SmoI CTYRAG 8 cut(s) 689, 842, 1008, 2839, 3402, 3524, 3809, 3869
SphI GCATGC 1 cut(s) 3232
SspDI GGCGCC 3 cut(s) 754, 895, 2974
SspMI CTAG 6 cut(s) 423, 678, 1020, 1575, 2546, 3120
SstI GAGCTC 4 cut(s) 36, 168, 1183, 3896
StyD4I CCNGG 7 cut(s) 231, 294, 394, 1239, 1509, 1864, 3682
StyI CCWWGG 8 cut(s) 144, 219, 318, 1019, 1721, 2316, 2774, 3912
TaaI ACNGT 7 cut(s) 467, 601, 1636, 2996, 3320, 3646, 3667
TaiI ACGT 4 cut(s) 1067, 2654, 3185, 3753
TatI WGTACW 3 cut(s) 1663, 2589, 3197
TauI GCSGC 9 cut(s) 173, 215, 218, 372, 419, 422, 1475, 3081, 3463
TscAI CASTG 4 cut(s) 472, 1497, 3343, 3810
TseFI GTSAC 4 cut(s) 25, 383, 2422, 2901
Tsp45I GTSAC 4 cut(s) 25, 383, 2422, 2901
TspDTI ATGAA 9 cut(s) 545, 1299, 1319, 2025, 2649, 3120, 3366, 3624, 3833
TspGWI ACGGA 2 cut(s) 1922, 2783
TspRI CASTG 4 cut(s) 472, 1497, 3343, 3810
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 4 cut(s) 148, 199, 405, 886
XapI RAATTY 8 cut(s) 609, 868, 1195, 1734, 1775, 2155, 2521, 3946
XbaI TCTAGA 1 cut(s) 3119
XceI RCATGY 3 cut(s) 2167, 3232, 3601
XcmI CCANNNNNNNNNTGG 1 cut(s) 1869
XmaJI CCTAGG 1 cut(s) 1019
XmiI GTMKAC 1 cut(s) 3629
XspI CTAG 6 cut(s) 423, 678, 1020, 1575, 2546, 3120
ZrmI AGTACT 1 cut(s) 1665
Zsp2I ATGCAT 1 cut(s) 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.