Rh4CG255700

Condensin-2 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012287
Physical Location & Seq
Forward (+)
50745311 .. 50751660
6350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG255700.1

Sequence Viewer

Length: 3099 bp
ATGGAAGAAACCATAACCCGAATAGTCACAGAGCTCGAAGACCTCCGCCACCTCGAAAACCCCACCAACACCCAATCCCAACCCATCTCCGATTCCACTCTCTCCGACCTCCAAACCCTCTTCGACTCCAATGACCCCGACCCCATGGACCGCTTCTCCGACGAGCTCGCCGCCAAGTCCCTCTCCCTCTCCAATCTGGTCCACCCCATCGCCGCCGCCATGGATTCCGGCCCGGCCCATTTCACCCTCTCCGCCTCCAATCTCTACCTCTCTCTCCTCCTCTCCCCCAATTCCCCCGTCTGCACTCTCTTCACCCCCATGGCGTTCTTCTCTCTCCTCCAGTCGATTCGCCGGTCGCTGAAGCACCGCCGCGCCGAATCGGGTCACGGCGAGCCCGGCACGGGGTCCGGTAACGCGGCCGCTAGGAAGCGGAAGGGGCGAGGCAAAGGTAAGGGTTTGAAGAACAGTGAGGATTGCGAGAGCGAAGAGAGGGAGTTTGATGTTAGGGTTTTGTTCTCGGTGCTTGAAAAATTGGAACTTGTGATGGATTTGGTTCATTTGGATCGGTTTCCGGATAGTTTGAAGTCGTTGGTTCAAACCGTGGCTGAAATTCCAGTCATGGGGTTTGAGGTGTGTGGCAATTCTGGTAATTATAACAAATTGACTGATTTGTGCTCTAGGGTTTTGCTTAAGGTGTTCAAGCCTGAGCATGGAGACCATGCCAACATTGCAGCTGAGGTGTTCAAGTCATTGGCGCCGATGATTCTTCTGCATAAATCGCAGGTGAGGATATTTGCGTTGAGTTTTGTGGGGAATCAGATGATGGGTGTGGCTAAGAATCTTGAGGGGGTGAAGAAAGCAATGGTGAATTTTCTGTGGTACTTGGTCCACAAGGCGCCGGATAAGTCTGAGCCTCGGGCTTTGGCTGTCGAGTCTATAATGGAGATTGTTAGAGTTATGGAGGTTCAGGAGCAAGTTGTGTTTGTGGAGTATGCGGTGAAGATGACTCAAGGGAAGGCTAGGTTGAGGCTTTTGGCTGTTGACCTTATTCTGGTGCTGGTTACATCTTTGAGAGATGTGATGGATTTGAATTCGGAGAGTGAAGTGAGGGATTCATTGGGTTGGAAATGCTTGGAGGGTTTGATTCAGCGCTGTTCGGATGCAATGGCTGGGATTCGAGCTCGTGCTTTGTCGAATTTGTCTCAGCTAGTTGGCTTCTTGTCAGGTGATGATAGGAGCCGGGAAGTGTTGAAAGAAGTTATGGGGTTTGGTGATGCAATGGATGAAAGGCAGGAGGGTTGGATGAATGAGATTTTGAGGGCAAGGTGTATGGATGAGAAGGCAGCTGTAAGGAAGGCTGCACTTCTTTTGGTTACCAAGTTGATGGCCATTCTTGGTAGTGGCTTTGATAGAGACTTGATCAAGACTATGGGCATGGCTTGTTCTGATCCACTTGTTAGCATACGCAAAGCAGCAATTTCAGCTCTTTCAGTGGCTTTTAGAACATTCCTGGATGATAGCGTGGCTACTGAGTGGCTACATTCTGTTCCAGCTTTAATAACTGATAATGAGTCTAGCATTCAAGAAGAATGTGAAAACTTGTTTGTAGAGCTGGTATTGGAAAGGGTATCTGCAGTTGGTTCTGAAATCAATACTGACTCAGTACTTCCTGAAGGCATTTTAAGCCTTTTGAAAGAGATTTGCAATGGAGAAGTGGCACCTTGGGTGAAGAAAATTTTAACAAACTTGGGAAAGAAGAATCGACTGAAGCACAAATTTGCTACTGCACTTCAGAGTATCATAAGGACATCTGAGTCTCTCTGGCTCAGTCAATCCATGCCTATAGAGAAATGGACAGCCCCACCAGGTTCTTGGTTTCTTCTGTCAGAGGTGTCAGCATACCTTGCAAAAGCAGTTGACTGGGAGTTTCTCCGTCATCATTGGCAGCTTTTTGACAAGCATGGAGTGGGAGGGGAGATTCAGAGTCCACTTCCACAGGGATATGCACATGAAGAGGGAGGGGGTATAGAATCCAGTTCTGTTGCTTGGGCTGGGGATCGTGTTTTTCTCTTACAGACTATTTCTAATGTATCTGTTGAGCTGCCTTCTGAAGTTGGTGCAGAGTTAGCTCATGACTTGCTTAAAAGAATTGAAAATTTTAACATGCATTCAACAGAGGTTAATGCTCATGTAAAAGCACTCAGAACATTGTGCAAGCGGAAGGTTTCAGATCCAGAGCAGGCTGATACACTTGTTAAAAGATGCGTACATCAGATTGCCTCAAAAGCTTCCAAGATATTAGATAAGTATATATCCAAGGAGTCAAATACAACCCGAAAAGGTGGCTTCTTTACGCCACCTATAAGTGGGAGTAGAATGGGCAAGAGAGCCATAACCATGTCCAAGTCTTTGTCAAAAGTAGTGACAGCAGTCTATACAATTGGGTCTTTGGTTATTATTTGTCCATCTGCTGATATGAGCACCACCATTCCACTACTATATACCATCATCACTTCTGGGAATTCTGATCCAAAAGTCAATAAACTAGCAGGCTCTATAGGATCTCTAAAGCAGACAGCACCTTCTTTGTACATTCAAGCATGGTTGACTATGGGGAAGATTTGCCTTACAGATGAAAAAATTGCAAAGACTTATATTCCTCTCTTTGTGCAGGAACTTGAAAAGAGTGATTGTGCAGCCCTTCGCAACAATCTGGTAGTTGTGATGGCAGATTTTTGCATTCGTCATACTTCTTTAGTTGATAGTTATATACCAAGGATCACAAAATGCCTCCGTGATCCATGCGAACTTGTAAGAAGGCAGACATTTATACTGCTCTCAAGATTGTTGCAGAGGGATTATGTGAAGTGGAGGGGAGTTCTTTTCCTGAGATTTCTCTTGTCACTTGTTGATGAGTCAGAAAAGATAAAGCAGCTCGCCACCTTTCTTTTCAGCAATATCTTGAAAGCTAAGGCGCCTCTTCTCGGATATAACAGTTTTGTAGAAGCAATTTTTGTTCTGAATGACTGCCGTGCCCACAAGGGACATACTGGTGCTCAGGGTTCACGAGCAGAGAGCCGCCTTTTTTCCATTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cnd1

Protein Analysis

1032

Amino Acids

114.49

Weight (kDa)

8.12

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cnd1 PF12717 901 - 1010 2e-17 non-SMC mitotic condensation complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15890 AT4G15890
fragaria_vesca FvH4_4g18310
malus_domestica MD16G1167900.v1.1
prunus_persica Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1 Prupe.1G134700_v2.0.a1
pyrus_communis pycom16g14150
rosa_chinensis RchiOBHm_Chr4g0422791
rosa_laevigata RLG00000007605
rosa_multiflora Rmu_sc0000830.1_g000049
rosa_roxburghii Rroxscaffold_5G00364640
rosa_rugosa Rorug04G0181400
rosa_samantha Rh4AG241800 Rh4BG245200 Rh4CG255700 Rh4DG240400
rosa_wichuraiana Rw4G020810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AarI CACCTGC 1 cut(s) 772
AasI GACNNNNNNGTC 1 cut(s) 1813
Acc36I ACCTGC 1 cut(s) 772
AccB1I GGYRCC 4 cut(s) 754, 895, 1717, 2974
AccII CGCG 2 cut(s) 372, 416
AccIII TCCGGA 1 cut(s) 571
AclWI GGATC 8 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792
AcoI YGGCCR 2 cut(s) 417, 1386
AcsI RAATTY 8 cut(s) 609, 868, 1090, 1195, 1734, 1775, 2155, 2521
AcuI CTGAAG 5 cut(s) 380, 1692, 1775, 1787, 2130
AcyI GRCGYC 3 cut(s) 755, 896, 2975
AfaI GTAC 4 cut(s) 881, 1665, 2268, 2591
AfeI AGCGCT 1 cut(s) 1151
AfiI CCNNNNNNNGG 7 cut(s) 401, 402, 620, 710, 1051, 2366, 2984
AflII CTTAAG 1 cut(s) 689
AjnI CCWGG 2 cut(s) 1509, 1864
Alw21I GWGCWC 6 cut(s) 36, 168, 677, 1183, 2483, 3058
Alw26I GTCTC 4 cut(s) 708, 1206, 1407, 1821
AlwI GGATC 8 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792
Ama87I CYCGRG 1 cut(s) 915
Aor13HI TCCGGA 1 cut(s) 571
Aor51HI AGCGCT 1 cut(s) 1151
AoxI GGCC 4 cut(s) 229, 234, 417, 1386
ApeKI GCWGC 8 cut(s) 731, 1343, 1358, 1472, 1945, 2101, 2696, 2932
ApoI RAATTY 8 cut(s) 609, 868, 1090, 1195, 1734, 1775, 2155, 2521
AspLEI GCGC 5 cut(s) 374, 757, 898, 1152, 2977
AspS9I GGNCC 6 cut(s) 148, 199, 230, 235, 405, 886
AsuC2I CCSGG 3 cut(s) 233, 396, 1241
AsuHPI GGTGA 9 cut(s) 235, 304, 796, 862, 877, 1009, 1238, 1283, 1738
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 4 cut(s) 148, 199, 405, 886
BaeGI GKGCMC 1 cut(s) 3037
BaeI ACNNNNGTAYC 2 cut(s) 2238, 2271
BalI TGGCCA 1 cut(s) 1388
BanI GGYRCC 4 cut(s) 754, 895, 1717, 2974
BanII GRGCYC 4 cut(s) 36, 168, 396, 1183
BauI CACGAG 2 cut(s) 1182, 3066
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 6 cut(s) 36, 168, 677, 1183, 2483, 3058
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 8 cut(s) 743, 1345, 1355, 1484, 1957, 2088, 2708, 2944
BccI CCATC 9 cut(s) 92, 215, 538, 817, 1075, 1378, 2473, 2513, 2719
BceAI ACGGC 2 cut(s) 403, 3015
BciT130I CCWGG 2 cut(s) 1511, 1866
BclI TGATCA 1 cut(s) 1419
BcnI CCSGG 3 cut(s) 233, 396, 1241
BcoDI GTCTC 4 cut(s) 708, 1206, 1407, 1821
BfaI CTAG 6 cut(s) 423, 678, 1020, 1208, 1575, 2546
BfmI CTRYAG 3 cut(s) 1632, 1842, 2556
BfoI RGCGCY 4 cut(s) 758, 899, 1153, 2978
BfrI CTTAAG 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 772
BmcAI AGTACT 1 cut(s) 1665
Bme1390I CCNGG 5 cut(s) 233, 396, 1241, 1511, 1866
Bme18I GGWCC 4 cut(s) 148, 199, 405, 886
BmeT110I CYCGRG 1 cut(s) 915
BmgT120I GGNCC 6 cut(s) 148, 199, 230, 235, 405, 886
BmiI GGNNCC 6 cut(s) 406, 756, 897, 1238, 1719, 2976
BmrFI CCNGG 5 cut(s) 233, 396, 1241, 1511, 1866
BmrI ACTGGG 1 cut(s) 1930
BmsI GCATC 3 cut(s) 1150, 1264, 2252
BmuI ACTGGG 1 cut(s) 1930
BoxI GACNNNNGTC 1 cut(s) 2429
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 4 cut(s) 705, 735, 2970, 3057
BpuEI CTTGAG 3 cut(s) 863, 993, 2824
BpuMI CCSGG 3 cut(s) 233, 396, 1241
BsaHI GRCGYC 3 cut(s) 755, 896, 2975
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 8 cut(s) 144, 219, 318, 600, 914, 1721, 2316, 2774
BsaWI WCCGGW 2 cut(s) 407, 571
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 7 cut(s) 401, 402, 620, 710, 1051, 2366, 2984
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 5 cut(s) 340, 614, 1925, 2034, 3055
Bse3DI GCAATG 5 cut(s) 726, 867, 1170, 1284, 1711
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 2 cut(s) 1511, 1866
BseDI CCNNGG 8 cut(s) 144, 219, 318, 600, 914, 1721, 2316, 2774
BseGI GGATG 5 cut(s) 1165, 1288, 1308, 1339, 1519
BseLI CCNNNNNNNGG 7 cut(s) 401, 402, 620, 710, 1051, 2366, 2984
BseMI GCAATG 5 cut(s) 726, 867, 1170, 1284, 1711
BseNI ACTGG 5 cut(s) 340, 614, 1925, 2034, 3055
BseRI GAGGAG 3 cut(s) 266, 269, 326
BseSI GKGCMC 1 cut(s) 3037
BseX3I CGGCCG 1 cut(s) 417
BseXI GCAGC 8 cut(s) 743, 1345, 1355, 1484, 1957, 2088, 2708, 2944
BseYI CCCAGC 2 cut(s) 1169, 2051
BsgI GTGCAG 6 cut(s) 286, 1344, 1770, 2139, 2690, 2715
Bsh1236I CGCG 2 cut(s) 372, 416
Bsh1285I CGRYCG 2 cut(s) 356, 420
BshFI GGCC 4 cut(s) 231, 236, 419, 1388
BshNI GGYRCC 4 cut(s) 754, 895, 1717, 2974
BsiEI CGRYCG 2 cut(s) 356, 420
BsiHKAI GWGCWC 6 cut(s) 36, 168, 677, 1183, 2483, 3058
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 8 cut(s) 228, 233, 352, 396, 408, 572, 899, 1240
BslFI GGGAC 2 cut(s) 163, 3057
BslI CCNNNNNNNGG 7 cut(s) 401, 402, 620, 710, 1051, 2366, 2984
BsmAI GTCTC 4 cut(s) 708, 1206, 1407, 1821
BsmFI GGGAC 2 cut(s) 163, 3057
BsmI GAATGC 3 cut(s) 1578, 2167, 2739
BsnI GGCC 4 cut(s) 231, 236, 419, 1388
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 1 cut(s) 915
Bsp1286I GDGCHC 8 cut(s) 36, 168, 396, 677, 1183, 2483, 3037, 3058
Bsp13I TCCGGA 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 2589
Bsp143I GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
Bsp19I CCATGG 3 cut(s) 144, 219, 318
BspANI GGCC 4 cut(s) 231, 236, 419, 1388
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 2 cut(s) 372, 416
BspHI TCATGA 1 cut(s) 2131
BspLI GGNNCC 6 cut(s) 406, 756, 897, 1238, 1719, 2976
BspMAI CTGCAG 1 cut(s) 1636
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 8 cut(s) 570, 1442, 2064, 2225, 2522, 2569, 2786, 2792
BspT107I GGYRCC 4 cut(s) 754, 895, 1717, 2974
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 5 cut(s) 726, 867, 1170, 1284, 1711
BsrFI RCCGGY 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 2589
BsrI ACTGG 5 cut(s) 340, 614, 1925, 2034, 3055
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 8 cut(s) 144, 219, 318, 600, 914, 1721, 2316, 2774
BssMI GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
BssNI GRCGYC 3 cut(s) 755, 896, 2975
BssSI CACGAG 2 cut(s) 1182, 3066
BssT1I CCWWGG 6 cut(s) 144, 219, 318, 1721, 2316, 2774
Bst2BI CACGAG 2 cut(s) 1182, 3066
Bst2UI CCWGG 2 cut(s) 1511, 1866
Bst4CI ACNGT 3 cut(s) 467, 601, 2996
Bst6I CTCTTC 5 cut(s) 125, 314, 480, 2007, 2985
BstACI GRCGYC 3 cut(s) 755, 896, 2975
BstAFI CTTAAG 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 1904
BstAUI TGTACA 1 cut(s) 2589
BstC8I GCNNGC 6 cut(s) 168, 392, 2216, 2241, 2551, 2937
BstDSI CCRYGG 4 cut(s) 144, 219, 318, 600
BstEII GGTNACC 1 cut(s) 1372
BstF5I GGATG 5 cut(s) 1165, 1288, 1308, 1339, 1519
BstFNI CGCG 2 cut(s) 372, 416
BstH2I RGCGCY 4 cut(s) 758, 899, 1153, 2978
BstHHI GCGC 5 cut(s) 374, 757, 898, 1152, 2977
BstKTI GATC 9 cut(s) 565, 1422, 1450, 2059, 2233, 2530, 2564, 2781, 2800
BstMAI GTCTC 4 cut(s) 708, 1206, 1407, 1821
BstMBI GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
BstMCI CGRYCG 2 cut(s) 356, 420
BstMWI GCNNNNNNNGC 9 cut(s) 396, 436, 728, 778, 1481, 1683, 1904, 2126, 2285
BstNI CCWGG 2 cut(s) 1511, 1866
BstNSI RCATGY 1 cut(s) 2167
BstPAI GACNNNNGTC 1 cut(s) 2429
BstPI GGTNACC 1 cut(s) 1372
BstSCI CCNGG 5 cut(s) 231, 394, 1239, 1509, 1864
BstSFI CTRYAG 3 cut(s) 1632, 1842, 2556
BstSLI GKGCMC 1 cut(s) 3037
BstUI CGCG 2 cut(s) 372, 416
BstV1I GCAGC 8 cut(s) 743, 1345, 1355, 1484, 1957, 2088, 2708, 2944
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 2230, 2561
BstXI CCANNNNNNTGG 2 cut(s) 1384, 1872
BstYI RGATCY 2 cut(s) 2230, 2561
BstZI CGGCCG 1 cut(s) 417
BsuRI GGCC 4 cut(s) 231, 236, 419, 1388
BtgI CCRYGG 4 cut(s) 144, 219, 318, 600
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 5 cut(s) 1165, 1288, 1308, 1339, 1519
BtsIMutI CAGTG 2 cut(s) 472, 1497
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 6 cut(s) 168, 392, 2216, 2241, 2551, 2937
CciI TCATGA 1 cut(s) 2131
CciNI GCGGCCGC 1 cut(s) 417
CfoI GCGC 5 cut(s) 374, 757, 898, 1152, 2977
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 6 cut(s) 148, 199, 230, 235, 405, 886
CsiI ACCWGGT 1 cut(s) 1864
Csp6I GTAC 4 cut(s) 880, 1664, 2267, 2590
CviQI GTAC 4 cut(s) 880, 1664, 2267, 2590
DinI GGCGCC 3 cut(s) 756, 897, 2976
DpnI GATC 9 cut(s) 564, 1421, 1449, 2058, 2232, 2529, 2563, 2780, 2799
DpnII GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
DrdI GACNNNNNNGTC 1 cut(s) 1813
DseDI GACNNNNNNGTC 1 cut(s) 1813
EaeI YGGCCR 2 cut(s) 417, 1386
EagI CGGCCG 1 cut(s) 417
Eam1104I CTCTTC 5 cut(s) 125, 314, 480, 2007, 2985
EarI CTCTTC 5 cut(s) 125, 314, 480, 2007, 2985
EciI GGCGGA 2 cut(s) 35, 241
Ecl136II GAGCTC 3 cut(s) 34, 166, 1181
EclXI CGGCCG 1 cut(s) 417
Eco130I CCWWGG 6 cut(s) 144, 219, 318, 1721, 2316, 2774
Eco24I GRGCYC 4 cut(s) 36, 168, 396, 1183
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 4 cut(s) 148, 199, 405, 886
Eco47III AGCGCT 1 cut(s) 1151
Eco52I CGGCCG 1 cut(s) 417
Eco53kI GAGCTC 3 cut(s) 34, 166, 1181
Eco57I CTGAAG 5 cut(s) 380, 1692, 1775, 1787, 2130
Eco88I CYCGRG 1 cut(s) 915
Eco91I GGTNACC 1 cut(s) 1372
EcoICRI GAGCTC 3 cut(s) 34, 166, 1181
EcoO65I GGTNACC 1 cut(s) 1372
EcoRI GAATTC 2 cut(s) 1090, 2521
EcoRII CCWGG 2 cut(s) 1509, 1864
EcoT14I CCWWGG 6 cut(s) 144, 219, 318, 1721, 2316, 2774
EcoT22I ATGCAT 1 cut(s) 2169
EcoT38I GRGCYC 4 cut(s) 36, 168, 396, 1183
EgeI GGCGCC 3 cut(s) 756, 897, 2976
EheI GGCGCC 3 cut(s) 756, 897, 2976
ErhI CCWWGG 6 cut(s) 144, 219, 318, 1721, 2316, 2774
FaqI GGGAC 2 cut(s) 163, 3057
FbaI TGATCA 1 cut(s) 1419
FokI GGATG 5 cut(s) 1172, 1295, 1315, 1346, 1526
FriOI GRGCYC 4 cut(s) 36, 168, 396, 1183
FspBI CTAG 6 cut(s) 423, 678, 1020, 1208, 1575, 2546
GlaI GCGC 5 cut(s) 373, 756, 897, 1151, 2976
GsaI CCCAGC 2 cut(s) 1173, 2055
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 4 cut(s) 758, 899, 1153, 2978
HaeIII GGCC 4 cut(s) 231, 236, 419, 1388
HapII CCGG 8 cut(s) 228, 233, 352, 396, 408, 572, 899, 1240
HhaI GCGC 5 cut(s) 374, 757, 898, 1152, 2977
Hin1I GRCGYC 3 cut(s) 755, 896, 2975
Hin6I GCGC 5 cut(s) 372, 755, 896, 1150, 2975
HinP1I GCGC 5 cut(s) 372, 755, 896, 1150, 2975
HincII GTYRAC 3 cut(s) 1042, 1918, 2607
HindII GTYRAC 3 cut(s) 1042, 1918, 2607
HindIII AAGCTT 1 cut(s) 2286
HpaII CCGG 8 cut(s) 228, 233, 352, 396, 408, 572, 899, 1240
HphI GGTGA 9 cut(s) 235, 304, 796, 862, 877, 1009, 1238, 1283, 1738
Hpy166II GTNNAC 7 cut(s) 202, 889, 1042, 1918, 1988, 2607, 3065
Hpy8I GTNNAC 7 cut(s) 202, 889, 1042, 1918, 1988, 2607, 3065
Hpy99I CGWCG 1 cut(s) 164
HpyCH4III ACNGT 3 cut(s) 467, 601, 2996
HpyF10VI GCNNNNNNNGC 9 cut(s) 396, 436, 728, 778, 1481, 1683, 1904, 2126, 2285
Hsp92I GRCGYC 3 cut(s) 755, 896, 2975
HspAI GCGC 5 cut(s) 372, 755, 896, 1150, 2975
KasI GGCGCC 3 cut(s) 754, 895, 2974
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1419
Kzo9I GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
LmnI GCTCC 2 cut(s) 970, 1236
Lsp1109I GCAGC 8 cut(s) 743, 1345, 1355, 1484, 1957, 2088, 2708, 2944
LweI GCATC 3 cut(s) 1150, 1264, 2252
MabI ACCWGGT 1 cut(s) 1864
MaeI CTAG 6 cut(s) 423, 678, 1020, 1208, 1575, 2546
MaeIII GTNAC 7 cut(s) 25, 383, 410, 1060, 1372, 2422, 2901
MalI GATC 9 cut(s) 564, 1421, 1449, 2058, 2232, 2529, 2563, 2780, 2799
MboI GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
MfeI CAATTG 1 cut(s) 2439
MflI RGATCY 2 cut(s) 2230, 2561
MhlI GDGCHC 8 cut(s) 36, 168, 396, 677, 1183, 2483, 3037, 3058
MlsI TGGCCA 1 cut(s) 1388
MluNI TGGCCA 1 cut(s) 1388
Mly113I GGCGCC 3 cut(s) 755, 896, 2975
MlyI GAGTC 9 cut(s) 119, 941, 1000, 1580, 1652, 1823, 1993, 2330, 2924
MmeI TCCRAC 4 cut(s) 129, 183, 1103, 1280
Mox20I TGGCCA 1 cut(s) 1388
Mph1103I ATGCAT 1 cut(s) 2169
MroI TCCGGA 1 cut(s) 571
MscI TGGCCA 1 cut(s) 1388
MseI TTAA 8 cut(s) 690, 1556, 1682, 1739, 2142, 2160, 2181, 2256
MslI CAYNNNNRTG 3 cut(s) 317, 2396, 3051
Msp20I TGGCCA 1 cut(s) 1388
MspA1I CMGCKG 2 cut(s) 734, 1346
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 8 cut(s) 228, 233, 352, 396, 408, 572, 899, 1240
MspR9I CCNGG 5 cut(s) 233, 396, 1241, 1511, 1866
MunI CAATTG 1 cut(s) 2439
Mva1269I GAATGC 3 cut(s) 1578, 2167, 2739
MvaI CCWGG 2 cut(s) 1511, 1866
MvnI CGCG 2 cut(s) 372, 416
MwoI GCNNNNNNNGC 9 cut(s) 396, 436, 728, 778, 1481, 1683, 1904, 2126, 2285
NarI GGCGCC 3 cut(s) 755, 896, 2975
NciI CCSGG 3 cut(s) 233, 396, 1241
NcoI CCATGG 3 cut(s) 144, 219, 318
NdeII GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
NlaIV GGNNCC 6 cut(s) 406, 756, 897, 1238, 1719, 2976
NmuCI GTSAC 4 cut(s) 25, 383, 2422, 2901
NotI GCGGCCGC 1 cut(s) 417
NsiI ATGCAT 1 cut(s) 2169
NspI RCATGY 1 cut(s) 2167
PagI TCATGA 1 cut(s) 2131
PaqCI CACCTGC 1 cut(s) 772
PctI GAATGC 3 cut(s) 1578, 2167, 2739
PfoI TCCNGGA 1 cut(s) 1509
PleI GAGTC 9 cut(s) 119, 940, 1000, 1579, 1652, 1822, 1992, 2329, 2923
PluTI GGCGCC 3 cut(s) 758, 899, 2978
PpsI GAGTC 9 cut(s) 119, 940, 1000, 1579, 1652, 1822, 1992, 2329, 2923
PshAI GACNNNNGTC 1 cut(s) 2429
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 3 cut(s) 36, 168, 1183
Psp6I CCWGG 2 cut(s) 1509, 1864
PspEI GGTNACC 1 cut(s) 1372
PspFI CCCAGC 2 cut(s) 1169, 2051
PspGI CCWGG 2 cut(s) 1509, 1864
PspN4I GGNNCC 6 cut(s) 406, 756, 897, 1238, 1719, 2976
PspPI GGNCC 6 cut(s) 148, 199, 230, 235, 405, 886
PstI CTGCAG 1 cut(s) 1636
PsuI RGATCY 2 cut(s) 2230, 2561
PvuII CAGCTG 2 cut(s) 734, 1346
RsaI GTAC 4 cut(s) 881, 1665, 2268, 2591
RsaNI GTAC 4 cut(s) 880, 1664, 2267, 2590
RseI CAYNNNNRTG 3 cut(s) 317, 2396, 3051
SacI GAGCTC 3 cut(s) 36, 168, 1183
SaqAI TTAA 8 cut(s) 690, 1556, 1682, 1739, 2142, 2160, 2181, 2256
Sau3AI GATC 9 cut(s) 562, 1419, 1447, 2056, 2230, 2527, 2561, 2778, 2797
Sau96I GGNCC 6 cut(s) 148, 199, 230, 235, 405, 886
ScaI AGTACT 1 cut(s) 1665
SchI GAGTC 9 cut(s) 119, 941, 1000, 1580, 1652, 1823, 1993, 2330, 2924
ScrFI CCNGG 5 cut(s) 233, 396, 1241, 1511, 1866
SduI GDGCHC 8 cut(s) 36, 168, 396, 677, 1183, 2483, 3037, 3058
SexAI ACCWGGT 1 cut(s) 1864
SfaNI GCATC 3 cut(s) 1150, 1264, 2252
SfcI CTRYAG 3 cut(s) 1632, 1842, 2556
SfoI GGCGCC 3 cut(s) 756, 897, 2976
SinI GGWCC 4 cut(s) 148, 199, 405, 886
SmiMI CAYNNNNRTG 3 cut(s) 317, 2396, 3051
SmlI CTYRAG 4 cut(s) 689, 842, 1008, 2839
SmoI CTYRAG 4 cut(s) 689, 842, 1008, 2839
SspDI GGCGCC 3 cut(s) 754, 895, 2974
SspMI CTAG 6 cut(s) 423, 678, 1020, 1208, 1575, 2546
SstI GAGCTC 3 cut(s) 36, 168, 1183
StyD4I CCNGG 5 cut(s) 231, 394, 1239, 1509, 1864
StyI CCWWGG 6 cut(s) 144, 219, 318, 1721, 2316, 2774
TaaI ACNGT 3 cut(s) 467, 601, 2996
TaqI TCGA 8 cut(s) 36, 54, 123, 344, 930, 1177, 1193, 1762
TatI WGTACW 2 cut(s) 1663, 2589
TauI GCSGC 7 cut(s) 173, 215, 218, 372, 419, 422, 3081
Tru1I TTAA 8 cut(s) 690, 1556, 1682, 1739, 2142, 2160, 2181, 2256
Tru9I TTAA 8 cut(s) 690, 1556, 1682, 1739, 2142, 2160, 2181, 2256
TscAI CASTG 2 cut(s) 472, 1497
TseFI GTSAC 4 cut(s) 25, 383, 2422, 2901
TseI GCWGC 8 cut(s) 731, 1343, 1358, 1472, 1945, 2101, 2696, 2932
Tsp45I GTSAC 4 cut(s) 25, 383, 2422, 2901
TspDTI ATGAA 6 cut(s) 545, 1104, 1299, 1319, 2025, 2649
TspGWI ACGGA 2 cut(s) 1922, 2783
TspRI CASTG 2 cut(s) 472, 1497
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 4 cut(s) 148, 199, 405, 886
XapI RAATTY 8 cut(s) 609, 868, 1090, 1195, 1734, 1775, 2155, 2521
XceI RCATGY 1 cut(s) 2167
XcmI CCANNNNNNNNNTGG 1 cut(s) 1869
XspI CTAG 6 cut(s) 423, 678, 1020, 1208, 1575, 2546
ZrmI AGTACT 1 cut(s) 1665
Zsp2I ATGCAT 1 cut(s) 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.