Rroxscaffold_5G00371990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018411
Physical Location & Seq
Reverse (-)
53462394 .. 53474881
12488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371990.1

Sequence Viewer

Length: 681 bp
ATGGGTTGGGTTAATTTTAGCCAGGGACGGATCCATGATGTGAAGTTTGGAGGATTACAATGCATAGAATCTCAATCATACAAGGAAAGTAATCGTACATTGAATAGGAATCTAGATCCTTCTAATCAAAGCCTCGAAGGCTCGAACTTGAAATTGCTGCGTTCGAGTTCCTTTGAGCCTCATTCTCGCTTCAATTTGCTGAGTTGCAGCAAAGTCTCTGCTTTTCAATTTGGTTTTGACAGATCTAAAACCACCATAACCACGCCCCCCCCCCCCCGCCCCCACGACAACCACTCACCTACACCGGCGTCGTTCAAGTACCAGCCTCCCGGGGAAGACCTCGACTCCTTGATCTCCGTCACCAACTCGACTCGGCTCGTCTTCAACCTCACCGAACAAGCACCGGACCTCGACCAACGCCTCCGTAACCTCGACTTCGACAAGAAGATGACACAGCAAAAGACAAGTCTTTCATCGTCTTGGACTTTGGGCTCAATCGCTCACGCAGCTCACACTCTACAACGATGGCAACCTCCGCATTTACGGCCTTCGATCCAAGTATTGCGGACTGGACGTTGTCCGGCAACTCCGGGGAGGGGCTGCGGTGGTGGTGGTGGGGCCGCGGTGGGAGCGATCCGCGGGAACGGGAGAGGGAGCGTGAGCAGCTTGGTGCGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

24.81

Weight (kDa)

10.23

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018411)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00931
rosa_laevigata RLG00000023083
rosa_multiflora Rmu_sc0001142.1_g000003 Rmu_sc0004143.1_g000013 Rmu_sc0007358.1_g000011
rosa_roxburghii Rroxscaffold_5G00371990 Rroxscaffold_7G00177740
rosa_samantha Rh1DG055600 Rh2CG269700 Rh5DG562600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 623, 639
AciI CCGC 8 cut(s) 277, 536, 565, 603, 621, 623, 637, 639
AclWI GGATC 5 cut(s) 25, 38, 110, 547, 628
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 2 cut(s) 97, 320
AfiI CCNNNNNNNGG 1 cut(s) 596
AgsI TTSAA 6 cut(s) 103, 151, 193, 227, 316, 385
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 2 cut(s) 509, 666
AluI AGCT 2 cut(s) 509, 666
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 5 cut(s) 25, 38, 110, 547, 628
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 545, 618
ApeKI GCWGC 5 cut(s) 157, 207, 506, 600, 663
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 2 cut(s) 406, 618
AsuC2I CCSGG 3 cut(s) 330, 331, 591
AsuHPI GGTGA 3 cut(s) 288, 352, 382
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 406
BamHI GGATCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 342, 373
BbvI GCAGC 5 cut(s) 144, 219, 518, 587, 675
BccI CCATC 1 cut(s) 519
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 3 cut(s) 330, 331, 591
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 113
BglI GCCNNNNNGGC 1 cut(s) 138
BglII AGATCT 1 cut(s) 242
BisI GCNGC 6 cut(s) 158, 208, 507, 601, 621, 664
BlsI GCNGC 6 cut(s) 159, 209, 508, 602, 622, 665
Bme1390I CCNGG 4 cut(s) 23, 330, 331, 591
Bme18I GGWCC 1 cut(s) 406
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 2 cut(s) 406, 618
BmiI GGNNCC 2 cut(s) 32, 619
BmrFI CCNGG 4 cut(s) 23, 330, 331, 591
BpiI GAAGAC 2 cut(s) 342, 373
BpuMI CCSGG 3 cut(s) 330, 331, 591
BsaHI GRCGYC 1 cut(s) 308
BsaJI CCNNGG 6 cut(s) 22, 329, 330, 590, 621, 637
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse118I RCCGGY 2 cut(s) 304, 675
Bse1I ACTGG 1 cut(s) 574
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 6 cut(s) 22, 329, 330, 590, 621, 637
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 574
BseXI GCAGC 5 cut(s) 144, 219, 518, 587, 675
Bsh1236I CGCG 2 cut(s) 623, 639
BshFI GGCC 2 cut(s) 547, 620
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 6 cut(s) 305, 330, 404, 581, 590, 676
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 39
BsnI GGCC 2 cut(s) 547, 620
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 6 cut(s) 30, 115, 242, 351, 552, 633
BspACI CCGC 8 cut(s) 277, 536, 565, 603, 621, 623, 637, 639
BspANI GGCC 2 cut(s) 547, 620
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 2 cut(s) 623, 639
BspLI GGNNCC 2 cut(s) 32, 619
BspPI GGATC 5 cut(s) 25, 38, 110, 547, 628
BsrFI RCCGGY 2 cut(s) 304, 675
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 2 cut(s) 304, 675
BssECI CCNNGG 6 cut(s) 22, 329, 330, 590, 621, 637
BssMI GATC 6 cut(s) 30, 115, 242, 351, 552, 633
BssNI GRCGYC 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 200
BstDSI CCRYGG 2 cut(s) 621, 637
BstFNI CGCG 2 cut(s) 623, 639
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 6 cut(s) 33, 118, 245, 354, 555, 636
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 6 cut(s) 30, 115, 242, 351, 552, 633
BstMWI GCNNNNNNNGC 7 cut(s) 138, 506, 535, 544, 629, 663, 672
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 4 cut(s) 21, 328, 329, 589
BstUI CGCG 2 cut(s) 623, 639
BstV1I GCAGC 5 cut(s) 144, 219, 518, 587, 675
BstV2I GAAGAC 2 cut(s) 342, 373
BstX2I RGATCY 3 cut(s) 30, 115, 242
BstYI RGATCY 3 cut(s) 30, 115, 242
BsuRI GGCC 2 cut(s) 547, 620
BtgI CCRYGG 2 cut(s) 621, 637
CfoI GCGC 1 cut(s) 675
Cfr10I RCCGGY 2 cut(s) 304, 675
Cfr13I GGNCC 2 cut(s) 406, 618
Cfr42I CCGCGG 2 cut(s) 624, 640
Cfr9I CCCGGG 1 cut(s) 329
CseI GACGC 1 cut(s) 297
Csp6I GTAC 2 cut(s) 96, 319
CviAII CATG 1 cut(s) 35
CviQI GTAC 2 cut(s) 96, 319
DdeI CTNAG 1 cut(s) 200
DpnI GATC 6 cut(s) 32, 117, 244, 353, 554, 635
DpnII GATC 6 cut(s) 30, 115, 242, 351, 552, 633
Eco24I GRGCYC 1 cut(s) 494
Eco47I GGWCC 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 494
FaeI CATG 1 cut(s) 38
FaiI YATR 4 cut(s) 36, 65, 79, 257
FaqI GGGAC 1 cut(s) 39
FatI CATG 1 cut(s) 34
FauI CCCGC 2 cut(s) 284, 632
Fnu4HI GCNGC 6 cut(s) 158, 208, 507, 601, 621, 664
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 6 cut(s) 158, 208, 507, 601, 621, 664
FspBI CTAG 1 cut(s) 113
GlaI GCGC 1 cut(s) 674
GluI GCNGC 6 cut(s) 158, 208, 507, 601, 621, 664
HaeIII GGCC 2 cut(s) 547, 620
HapII CCGG 6 cut(s) 305, 330, 404, 581, 590, 676
HgaI GACGC 1 cut(s) 297
HhaI GCGC 1 cut(s) 675
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HinfI GANTC 4 cut(s) 68, 109, 344, 370
HpaII CCGG 6 cut(s) 305, 330, 404, 581, 590, 676
HphI GGTGA 3 cut(s) 288, 352, 382
Hpy188III TCNNGA 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 3 cut(s) 129, 131, 558
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 2 cut(s) 63, 207
HpyF10VI GCNNNNNNNGC 7 cut(s) 138, 506, 535, 544, 629, 663, 672
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 574
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 1 cut(s) 673
KspI CCGCGG 2 cut(s) 624, 640
Kzo9I GATC 6 cut(s) 30, 115, 242, 351, 552, 633
LmnI GCTCC 2 cut(s) 629, 654
LpnPI CCDG 9 cut(s) 8, 35, 318, 335, 343, 417, 555, 594, 603
Lsp1109I GCAGC 5 cut(s) 144, 219, 518, 587, 675
MaeI CTAG 1 cut(s) 113
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 2 cut(s) 358, 425
MalI GATC 6 cut(s) 32, 117, 244, 353, 554, 635
MboI GATC 6 cut(s) 30, 115, 242, 351, 552, 633
MboII GAAGA 3 cut(s) 347, 373, 457
MflI RGATCY 3 cut(s) 30, 115, 242
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 4 cut(s) 13, 152, 193, 227
MlyI GAGTC 2 cut(s) 338, 364
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 1 cut(s) 12
MspA1I CMGCKG 2 cut(s) 623, 639
MspI CCGG 6 cut(s) 305, 330, 404, 581, 590, 676
MspR9I CCNGG 4 cut(s) 23, 330, 331, 591
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 623, 639
MwoI GCNNNNNNNGC 7 cut(s) 138, 506, 535, 544, 629, 663, 672
NciI CCSGG 3 cut(s) 330, 331, 591
NdeII GATC 6 cut(s) 30, 115, 242, 351, 552, 633
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 2 cut(s) 32, 619
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 65
PfeI GAWTC 2 cut(s) 68, 109
PflFI GACNNNGTC 1 cut(s) 576
PkrI GCNGC 6 cut(s) 159, 209, 508, 602, 622, 665
PleI GAGTC 2 cut(s) 338, 364
PpsI GAGTC 2 cut(s) 338, 364
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 32, 619
PspPI GGNCC 2 cut(s) 406, 618
PsuI RGATCY 3 cut(s) 30, 115, 242
PsyI GACNNNGTC 1 cut(s) 576
RsaI GTAC 2 cut(s) 97, 320
RsaNI GTAC 2 cut(s) 96, 319
SacII CCGCGG 2 cut(s) 624, 640
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 6 cut(s) 158, 208, 507, 601, 621, 664
Sau3AI GATC 6 cut(s) 30, 115, 242, 351, 552, 633
Sau96I GGNCC 2 cut(s) 406, 618
SchI GAGTC 2 cut(s) 338, 364
ScrFI CCNGG 4 cut(s) 23, 330, 331, 591
SduI GDGCHC 1 cut(s) 494
SetI ASST 9 cut(s) 301, 342, 390, 411, 432, 511, 535, 577, 668
Sfr303I CCGCGG 2 cut(s) 624, 640
SgrAI CRCCGGYG 1 cut(s) 304
SgrBI CCGCGG 2 cut(s) 624, 640
SinI GGWCC 1 cut(s) 406
SmaI CCCGGG 1 cut(s) 331
Sse9I AATT 4 cut(s) 13, 152, 193, 227
SsiI CCGC 8 cut(s) 277, 536, 565, 603, 621, 623, 637, 639
SspMI CTAG 1 cut(s) 113
StyD4I CCNGG 4 cut(s) 21, 328, 329, 589
TaiI ACGT 1 cut(s) 577
TaqI TCGA 9 cut(s) 135, 143, 164, 342, 368, 411, 432, 438, 551
TasI AATT 4 cut(s) 13, 152, 193, 227
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 2 cut(s) 68, 109
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 5 cut(s) 157, 207, 506, 600, 663
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 462
TspGWI ACGGA 3 cut(s) 43, 346, 413
TspMI CCCGGG 1 cut(s) 329
Tth111I GACNNNGTC 1 cut(s) 576
VpaK11BI GGWCC 1 cut(s) 406
XbaI TCTAGA 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 329
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.