Rroxscaffold_7G00177740

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018411
Physical Location & Seq
Forward (+)
17326042 .. 17326465
424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177740.1

Sequence Viewer

Length: 327 bp
ATGCCCCGCCCCCACGACAACCAGCTCACCTACACCGGTGGTGAGACCAAGATCCTCGCCCTCGAATGCACCATCAAGTTCCTCGCCCTCCTCGCCGGGGTCTCCGCCCTCTCCAACGTACCGGGACCCGACCGACGGCCTCCGAACCTCGACTTCAACGGCAAGATGGCGCGGGCAAACGACAAGTCGTTCATAGTCTCGGACTTCGAGCTCAACCGCTCACACGGGCTCACACTCGACAACAATGGCAACCTTCGGGGAGGGGCTGCGGTGGTGGTGGCGGCGGCTACGGTGGGAGCGGTCTGCGAGACGGGAGAGGGAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

108

Amino Acids

11.28

Weight (kDa)

6.05

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018411)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00931
rosa_laevigata RLG00000023083
rosa_multiflora Rmu_sc0001142.1_g000003 Rmu_sc0004143.1_g000013 Rmu_sc0007358.1_g000011
rosa_roxburghii Rroxscaffold_5G00371990 Rroxscaffold_7G00177740
rosa_samantha Rh1DG055600 Rh2CG269700 Rh5DG562600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 219, 299
AccII CGCG 1 cut(s) 172
AciI CCGC 8 cut(s) 7, 105, 172, 217, 269, 281, 284, 299
AclWI GGATC 1 cut(s) 46
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 97, 135
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 157
AluBI AGCT 2 cut(s) 25, 211
AluI AGCT 2 cut(s) 25, 211
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 4 cut(s) 38, 106, 202, 302
AlwI GGATC 1 cut(s) 46
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AsiGI ACCGGT 1 cut(s) 35
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 2 cut(s) 97, 123
AsuHPI GGTGA 2 cut(s) 19, 53
AvaII GGWCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 213, 231
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 253
BccI CCATC 2 cut(s) 80, 160
BceAI ACGGC 2 cut(s) 152, 175
BcnI CCSGG 2 cut(s) 97, 123
BcoDI GTCTC 4 cut(s) 38, 106, 202, 302
BisI GCNGC 3 cut(s) 267, 282, 285
BlsI GCNGC 3 cut(s) 268, 283, 286
Bme1390I CCNGG 2 cut(s) 97, 123
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 126, 127
BmrFI CCNGG 2 cut(s) 97, 123
BpuMI CCSGG 2 cut(s) 97, 123
BsaI GGTCTC 2 cut(s) 38, 106
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 135
Bse118I RCCGGY 1 cut(s) 35
BseDI CCNNGG 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 97, 135
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 253
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 1 cut(s) 139
BshTI ACCGGT 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 3 cut(s) 36, 96, 122
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 97, 135
BsmAI GTCTC 4 cut(s) 38, 106, 202, 302
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 139
Bso31I GGTCTC 2 cut(s) 38, 106
Bsp1286I GDGCHC 2 cut(s) 213, 231
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 8 cut(s) 7, 105, 172, 217, 269, 281, 284, 299
BspANI GGCC 1 cut(s) 139
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 126, 127
BspPI GGATC 1 cut(s) 46
BspTNI GGTCTC 2 cut(s) 38, 106
BsrBI CCGCTC 2 cut(s) 219, 299
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 174
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 4 cut(s) 38, 106, 202, 302
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 95, 121
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 119
CspAI ACCGGT 1 cut(s) 35
CviJI RGCY 6 cut(s) 25, 139, 211, 229, 266, 287
CviKI_1 RGCY 6 cut(s) 25, 139, 211, 229, 266, 287
CviQI GTAC 1 cut(s) 119
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 213, 231
Eco31I GGTCTC 2 cut(s) 38, 106
Eco47I GGWCC 1 cut(s) 125
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 125
EcoT38I GRGCYC 2 cut(s) 213, 231
Esp3I CGTCTC 1 cut(s) 302
FaiI YATR 1 cut(s) 194
FaqI GGGAC 1 cut(s) 138
FauI CCCGC 2 cut(s) 14, 165
Fnu4HI GCNGC 3 cut(s) 267, 282, 285
FriOI GRGCYC 2 cut(s) 213, 231
Fsp4HI GCNGC 3 cut(s) 267, 282, 285
GlaI GCGC 1 cut(s) 171
GluI GCNGC 3 cut(s) 267, 282, 285
HaeIII GGCC 1 cut(s) 139
HapII CCGG 3 cut(s) 36, 96, 122
HhaI GCGC 1 cut(s) 172
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HpaII CCGG 3 cut(s) 36, 96, 122
HphI GGTGA 2 cut(s) 19, 53
Hpy188I TCNGA 2 cut(s) 144, 202
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 117
HspAI GCGC 1 cut(s) 170
KflI GGGWCCC 1 cut(s) 125
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 296, 320
LpnPI CCDG 4 cut(s) 35, 49, 109, 135
Lsp1109I GCAGC 1 cut(s) 253
MaeII ACGT 1 cut(s) 117
MalI GATC 1 cut(s) 53
MbiI CCGCTC 2 cut(s) 219, 299
MboI GATC 1 cut(s) 51
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 2 cut(s) 213, 231
MmeI TCCRAC 1 cut(s) 138
MspI CCGG 3 cut(s) 36, 96, 122
MspR9I CCNGG 2 cut(s) 97, 123
Mva1269I GAATGC 1 cut(s) 71
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 92
NciI CCSGG 2 cut(s) 97, 123
NdeII GATC 1 cut(s) 51
NlaIV GGNNCC 2 cut(s) 126, 127
PctI GAATGC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 3 cut(s) 268, 283, 286
PpuMI RGGWCCY 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 126, 127
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
SacI GAGCTC 1 cut(s) 213
SatI GCNGC 3 cut(s) 267, 282, 285
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 97, 123
SduI GDGCHC 2 cut(s) 213, 231
SetI ASST 6 cut(s) 27, 32, 120, 150, 213, 255
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 125
SsiI CCGC 8 cut(s) 7, 105, 172, 217, 269, 281, 284, 299
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 2 cut(s) 95, 121
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 63, 150, 207, 237
TaqII GACCGA 1 cut(s) 147
TauI GCSGC 2 cut(s) 284, 287
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.