Rh2CG269700

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018411
Physical Location & Seq
Forward (+)
29267466 .. 29271771
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG269700.1

Sequence Viewer

Length: 660 bp
ATGAAGTCGATCTGGGACACTGGCGAGCAGGACGAGGCCGCGCCGGTGAGCAGGATTGGGCAGCGTAAAAGCTGGGCACAGAAACAGGACCAAAATCCCATCCGGGTCGGGTCGAGATCTAAAACCACCATAATCACACGCCCCCGTCCCCGCCCCCATGACAACCAGCTCACCTACACCGGTGGTGAGACCAAGATCCTTTCTGTCGAATGCACCATCAAGTTCCCCGCCCTCGTCGCCAAGGTGTCCGCCTTATCAGACAGCGACGTGTCGTTCAAGTACCAGCTCCCCGGCGAAGACCTCGACTCCTTGATCTCCGTTACCAACTCAACTCAGCTCGTCTTCAACCTCACCGAACAGTACCAAGACCTGACCGATGCCCTCCAGAACCTCGACTTTGACGGCAAGATGACGCAGGCAAACGACAAGTCATTCATCATCTCGAACTTCAGGCTCAACCGCTCACGCAGGCTCACACTCGACAACGATGGCAACCTCCGCATTTATAGCTTCGATCCGAGTATTGTCGACTGGACAGTTGTCTGGAGACTTCAGGGAGGGGCTGCGGTGGTGGTGGTGGCGGCTGTGGTGGTGGCGGCTGCGGTGGGAGCGGTATGCGGGAACGGGAGACGGAGCGTGAGCAGCTTGGTGCGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

219

Amino Acids

24.25

Weight (kDa)

8.92

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 53 - 110 1.6e-07 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018411)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00931
rosa_laevigata RLG00000023083
rosa_multiflora Rmu_sc0001142.1_g000003 Rmu_sc0004143.1_g000013 Rmu_sc0007358.1_g000011
rosa_roxburghii Rroxscaffold_5G00371990 Rroxscaffold_7G00177740
rosa_samantha Rh1DG055600 Rh2CG269700 Rh5DG562600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 462, 611
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 41
AclWI GGATC 2 cut(s) 190, 509
AcuI CTGAAG 2 cut(s) 433, 536
AfaI GTAC 2 cut(s) 281, 362
AflIII ACRYGT 1 cut(s) 267
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 277, 346
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 6 cut(s) 72, 169, 286, 337, 510, 645
AluI AGCT 6 cut(s) 72, 169, 286, 337, 510, 645
Alw26I GTCTC 3 cut(s) 182, 541, 622
AlwI GGATC 2 cut(s) 190, 509
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 4 cut(s) 61, 563, 599, 642
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 43, 654
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 2 cut(s) 104, 291
AsuHPI GGTGA 4 cut(s) 58, 163, 197, 343
AvaII GGWCC 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 79
BbsI GAAGAC 2 cut(s) 303, 334
BbvI GCAGC 4 cut(s) 73, 550, 586, 654
BccI CCATC 3 cut(s) 107, 224, 482
BceAI ACGGC 1 cut(s) 418
BcnI CCSGG 2 cut(s) 104, 291
BcoDI GTCTC 3 cut(s) 182, 541, 622
BglII AGATCT 1 cut(s) 116
BisI GCNGC 7 cut(s) 39, 62, 564, 582, 597, 600, 643
BlsI GCNGC 7 cut(s) 40, 63, 565, 583, 598, 601, 644
Bme1390I CCNGG 2 cut(s) 104, 291
Bme18I GGWCC 1 cut(s) 88
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 88
BmrFI CCNGG 2 cut(s) 104, 291
BmsI GCATC 1 cut(s) 367
BoxI GACNNNNGTC 1 cut(s) 539
BpiI GAAGAC 2 cut(s) 303, 334
BpmI CTGGAG 2 cut(s) 368, 565
BpuMI CCSGG 2 cut(s) 104, 291
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 240, 289
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bse118I RCCGGY 2 cut(s) 43, 179
Bse1I ACTGG 2 cut(s) 25, 536
BseDI CCNNGG 2 cut(s) 240, 289
BseGI GGATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 25, 536
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 4 cut(s) 73, 550, 586, 654
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 179
BsiSI CCGG 4 cut(s) 44, 103, 180, 291
BslFI GGGAC 2 cut(s) 29, 132
BsmAI GTCTC 3 cut(s) 182, 541, 622
BsmBI CGTCTC 1 cut(s) 622
BsmFI GGGAC 2 cut(s) 29, 132
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 5 cut(s) 9, 116, 195, 312, 514
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 1 cut(s) 41
BspPI GGATC 2 cut(s) 190, 509
BspTNI GGTCTC 1 cut(s) 182
BsrBI CCGCTC 2 cut(s) 462, 611
BsrFI RCCGGY 2 cut(s) 43, 179
BsrI ACTGG 2 cut(s) 25, 536
BssAI RCCGGY 2 cut(s) 43, 179
BssECI CCNNGG 2 cut(s) 240, 289
BssMI GATC 5 cut(s) 9, 116, 195, 312, 514
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 360, 538
BstC8I GCNNGC 3 cut(s) 26, 417, 470
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 2 cut(s) 43, 654
BstKTI GATC 5 cut(s) 12, 119, 198, 315, 517
BstMAI GTCTC 3 cut(s) 182, 541, 622
BstMBI GATC 5 cut(s) 9, 116, 195, 312, 514
BstMWI GCNNNNNNNGC 6 cut(s) 236, 498, 507, 608, 642, 651
BstPAI GACNNNNGTC 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 102, 289
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 4 cut(s) 73, 550, 586, 654
BstV2I GAAGAC 2 cut(s) 303, 334
BstX2I RGATCY 2 cut(s) 116, 195
BstYI RGATCY 2 cut(s) 116, 195
BsuRI GGCC 1 cut(s) 38
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 3 cut(s) 26, 417, 470
CfoI GCGC 2 cut(s) 43, 654
Cfr10I RCCGGY 2 cut(s) 43, 179
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 280, 361
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 1 cut(s) 158
CviQI GTAC 2 cut(s) 280, 361
DdeI CTNAG 1 cut(s) 333
DpnI GATC 5 cut(s) 11, 118, 197, 314, 516
DpnII GATC 5 cut(s) 9, 116, 195, 312, 514
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 433, 536
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 622
FaeI CATG 1 cut(s) 161
FaiI YATR 4 cut(s) 131, 159, 507, 616
FaqI GGGAC 2 cut(s) 29, 132
FatI CATG 1 cut(s) 157
FauI CCCGC 3 cut(s) 158, 235, 611
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 7 cut(s) 39, 62, 564, 582, 597, 600, 643
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 7 cut(s) 39, 62, 564, 582, 597, 600, 643
GlaI GCGC 2 cut(s) 42, 653
GluI GCNGC 7 cut(s) 39, 62, 564, 582, 597, 600, 643
GsaI CCCAGC 1 cut(s) 76
GsuI CTGGAG 2 cut(s) 368, 565
HaeIII GGCC 1 cut(s) 38
HapII CCGG 4 cut(s) 44, 103, 180, 291
HgaI GACGC 1 cut(s) 421
HhaI GCGC 2 cut(s) 43, 654
Hin1II CATG 1 cut(s) 161
Hin6I GCGC 2 cut(s) 41, 652
HinP1I GCGC 2 cut(s) 41, 652
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 1 cut(s) 305
HpaII CCGG 4 cut(s) 44, 103, 180, 291
HphI GGTGA 4 cut(s) 58, 163, 197, 343
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 2 cut(s) 259, 519
Hpy188III TCNNGA 4 cut(s) 114, 385, 442, 544
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 2 cut(s) 239, 269
HpyCH4III ACNGT 2 cut(s) 360, 538
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 498, 507, 608, 642, 651
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 1 cut(s) 161
HspAI GCGC 2 cut(s) 41, 652
Kzo9I GATC 5 cut(s) 9, 116, 195, 312, 514
LmnI GCTCC 3 cut(s) 291, 608, 633
Lsp1109I GCAGC 4 cut(s) 73, 550, 586, 654
LweI GCATC 1 cut(s) 367
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 319
MalI GATC 5 cut(s) 11, 118, 197, 314, 516
MbiI CCGCTC 2 cut(s) 462, 611
MboI GATC 5 cut(s) 9, 116, 195, 312, 514
MboII GAAGA 2 cut(s) 308, 334
MflI RGATCY 2 cut(s) 116, 195
MhlI GDGCHC 1 cut(s) 79
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 8 cut(s) 28, 242, 311, 359, 392, 401, 506, 551
MspI CCGG 4 cut(s) 44, 103, 180, 291
MspR9I CCNGG 2 cut(s) 104, 291
Mva1269I GAATGC 1 cut(s) 215
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 6 cut(s) 236, 498, 507, 608, 642, 651
NciI CCSGG 2 cut(s) 104, 291
NdeII GATC 5 cut(s) 9, 116, 195, 312, 514
NlaIII CATG 1 cut(s) 161
PctI GAATGC 1 cut(s) 215
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 7 cut(s) 40, 63, 565, 583, 598, 601, 644
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PshAI GACNNNNGTC 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 2 cut(s) 116, 195
RsaI GTAC 2 cut(s) 281, 362
RsaNI GTAC 2 cut(s) 280, 361
SalI GTCGAC 1 cut(s) 527
SatI GCNGC 7 cut(s) 39, 62, 564, 582, 597, 600, 643
Sau3AI GATC 5 cut(s) 9, 116, 195, 312, 514
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 104, 291
SduI GDGCHC 1 cut(s) 79
SfaNI GCATC 1 cut(s) 367
SgrAI CRCCGGYG 2 cut(s) 43, 179
SinI GGWCC 1 cut(s) 88
StyD4I CCNGG 2 cut(s) 102, 289
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 2 cut(s) 360, 538
TaiI ACGT 1 cut(s) 270
TaqI TCGA 9 cut(s) 8, 113, 207, 303, 393, 443, 480, 513, 528
TaqII GACCGA 1 cut(s) 389
TauI GCSGC 3 cut(s) 41, 584, 599
TscAI CASTG 1 cut(s) 25
TseI GCWGC 4 cut(s) 61, 563, 599, 642
TspDTI ATGAA 2 cut(s) 17, 424
TspGWI ACGGA 2 cut(s) 307, 646
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.