Rroxscaffold_7G00156480

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009835
Physical Location & Seq
Forward (+)
331271 .. 335226
3956 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00156480.1

Sequence Viewer

Length: 519 bp
ATGGCGTTGGATTGTTTCTTTGCGGTTTGGTTCGTGGTTGGCAATGTTTGGATATTTGGAGGACACTCATCTGCAAATGATGCACCTAACCAGTACAGGTTATGTATAGTATTTCTGACATTTAGCTGTATCGGATACGCCATGCCTTTCATTTTATGTGCAACAATCTGCTGCTGTCTTCCCTGTATAATATCAGTTCTTGGGTTTCGAGAAGATCTGACACAGGCACGAGGAGCAACGCCTGAATCAATTAATGCTCTCCCAACATACAAATTCAAGTTGAAGAAAAACAGAAATGGTGATAACAGTGAAGGTGTTGTGGCTGCAGGAACTGAGAAGGAGCGGGTGATTTCTGGAGAGGATGCGGTTTGTTGCATCTGCTTGGCAAAATATGCAAACAATGACGAGCTGAGAGAGTTGCCGTGTTCTCATTTTTTCCACAAGGATTGTGTGGATAAGTGGTTGAAAATCAATGCGTCTTGTCCTCTCTGCAAGAGTGAGGTTGGTGAGAGCCTTTAG

Protein Analysis

172

Amino Acids

18.97

Weight (kDa)

5.9

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 165 3.5e-15 Ring finger domain
zf-RING_11 PF17123 124 - 151 1.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 124 - 164 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 124 - 164 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 125 - 165 8e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 343
AciI CCGC 3 cut(s) 23, 343, 365
AcsI RAATTY 1 cut(s) 272
AfaI GTAC 1 cut(s) 95
AgsI TTSAA 3 cut(s) 277, 283, 466
AluBI AGCT 2 cut(s) 126, 409
AluI AGCT 2 cut(s) 126, 409
AlwNI CAGNNNCTG 1 cut(s) 332
ApeKI GCWGC 2 cut(s) 171, 323
ApoI RAATTY 1 cut(s) 272
AseI ATTAAT 1 cut(s) 252
AsuHPI GGTGA 3 cut(s) 311, 358, 518
BauI CACGAG 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 158, 310
BceAI ACGGC 1 cut(s) 406
BciVI GTATCC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 128
BglII AGATCT 1 cut(s) 214
BisI GCNGC 2 cut(s) 172, 324
BlsI GCNGC 2 cut(s) 173, 325
BmsI GCATC 3 cut(s) 70, 352, 384
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 49
BseGI GGATG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 2 cut(s) 324, 401
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 2 cut(s) 158, 310
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 3 cut(s) 23, 343, 365
BspCNI CTCAG 2 cut(s) 325, 402
BspMAI CTGCAG 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 1 cut(s) 91
BssMI GATC 1 cut(s) 214
BssSI CACGAG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 308
BstAPI GCANNNNNTGC 2 cut(s) 80, 392
BstDEI CTNAG 2 cut(s) 333, 410
BstF5I GGATG 1 cut(s) 367
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 80, 233, 392
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 158, 310
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuI GTATCC 1 cut(s) 128
BtsCI GGATG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 332
CseI GACGC 1 cut(s) 465
Csp6I GTAC 1 cut(s) 94
CspCI CAANNNNNGTGG 2 cut(s) 428, 463
CviAII CATG 1 cut(s) 142
CviJI RGCY 4 cut(s) 126, 323, 409, 513
CviKI_1 RGCY 4 cut(s) 126, 323, 409, 513
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 333, 410
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
FaeI CATG 1 cut(s) 145
FaiI YATR 7 cut(s) 103, 107, 143, 157, 188, 268, 393
FatI CATG 1 cut(s) 141
FauI CCCGC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 172, 324
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 172, 324
GluI GCNGC 2 cut(s) 172, 324
GsuI CTGGAG 1 cut(s) 375
HgaI GACGC 1 cut(s) 465
Hin1II CATG 1 cut(s) 145
HinfI GANTC 1 cut(s) 245
HphI GGTGA 3 cut(s) 311, 358, 518
Hpy188I TCNGA 3 cut(s) 117, 134, 219
Hpy188III TCNNGA 2 cut(s) 209, 354
HpyAV CCTTC 2 cut(s) 305, 331
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4V TGCA 7 cut(s) 74, 83, 161, 326, 375, 395, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 233, 392
HpyF3I CTNAG 2 cut(s) 333, 410
Hsp92II CATG 1 cut(s) 145
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 2 cut(s) 233, 340
LpnPI CCDG 7 cut(s) 82, 104, 196, 209, 255, 312, 339
Lsp1109I GCAGC 2 cut(s) 158, 310
LweI GCATC 3 cut(s) 70, 352, 384
MalI GATC 1 cut(s) 216
MbiI CCGCTC 1 cut(s) 343
MboI GATC 1 cut(s) 214
MboII GAAGA 3 cut(s) 170, 224, 295
MflI RGATCY 1 cut(s) 214
MluCI AATT 2 cut(s) 249, 272
MnlI CCTC 5 cut(s) 53, 224, 352, 493, 495
MseI TTAA 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 80, 233, 392
NdeII GATC 1 cut(s) 214
NlaIII CATG 1 cut(s) 145
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 173, 325
PshBI ATTAAT 1 cut(s) 252
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 2 cut(s) 172, 324
Sau3AI GATC 1 cut(s) 214
SetI ASST 6 cut(s) 88, 101, 128, 316, 411, 504
SfaNI GCATC 3 cut(s) 70, 352, 384
SfcI CTRYAG 1 cut(s) 324
Sse9I AATT 2 cut(s) 249, 272
SsiI CCGC 3 cut(s) 23, 343, 365
TaaI ACNGT 1 cut(s) 308
TaqI TCGA 1 cut(s) 208
TasI AATT 2 cut(s) 249, 272
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 1 cut(s) 313
TseI GCWGC 2 cut(s) 171, 323
TspDTI ATGAA 1 cut(s) 139
TspRI CASTG 1 cut(s) 313
VspI ATTAAT 1 cut(s) 252
XapI RAATTY 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.