Rorug06G0399700

Transcription factor S-II (TFIIS), central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002519
Physical Location & Seq
Reverse (-)
54446676 .. 54448724
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0399700.1

Sequence Viewer

Length: 801 bp
ATGGCAGAAGCACCAAAACCCAACTCGGCGAGCAAGAATGATCAGTCGCTGACGCTTGAGAAGGTAAGACAGGCATTGATCAGCCAAGAAGACACCATCGTGTTTAGGCTGATTGAGAGAGCCGCCTTCCCTCTCAACTCTCCTACCTACCACCACAAAATTTCGTCTTCACCTTCTGTGTTTAACTTCATTGTCAAGAGCACCGAGGCCCTTCAATCTCAGGCCGGTAGATATGAAATCCCTGAAGAACTGCCTTTTTTCCCAGAAAACTTACCACCTTCATTGCTGCCGCCTCCTGCTGATCCACCGGTATTGCATCCTGCTGCAGCTTCCATTAATCTTAACAAAAAGATATTGGATATATATTTGAAACAATTGCTTCCTCTGTTTGCTGTCCCCGGTGATGATGGAAACTATGCAGCAACTGCCTCCAGTGATCTTGATTGTTTACAGGCAATCTCTAGAAGGATTCATTATGGATACTATGTTGCTGAGGTCAAATTCAGGGACGCCCCTCAAGATTATGAGCCTGCAATTCGCGCTCAGGACAGAGAAGGTCTCATGAAATTGTTGACATTTACGAATGTGGAAGAGCAGGTGAAGAAGAGAGTTGAGAAGAAAGCAGTGATCTTTGGGCAAGAAGTGAGCCTTGACAACAACACTAACAGTGTAAAACAAAATGGAACTGTAAATGGAAAATACAAGGTTGATCCGTCTCTAGTTTCTCGTCTCTATGGAGAATGGGTAATGCCTCTCACAAAGGATGTTCAAGTTGAGTACCTCCTTAGACGCCTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOC TFIIS_M

Protein Analysis

266

Amino Acids

29.87

Weight (kDa)

6.14

Isoelectric Point (pI)

57.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CM_2 PF01817 140 - 257 7.4e-08 Chorismate mutase type II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 586
Acc36I ACCTGC 1 cut(s) 586
AccII CGCG 1 cut(s) 540
AciI CCGC 2 cut(s) 123, 290
AclWI GGATC 2 cut(s) 296, 704
AcsI RAATTY 2 cut(s) 159, 500
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 510, 790
AfaI GTAC 1 cut(s) 779
AgeI ACCGGT 1 cut(s) 307
AgsI TTSAA 3 cut(s) 215, 370, 770
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 563, 720, 734
AlwI GGATC 2 cut(s) 296, 704
AlwNI CAGNNNCTG 2 cut(s) 49, 425
AoxI GGCC 2 cut(s) 207, 222
ApeKI GCWGC 4 cut(s) 286, 323, 326, 419
ApoI RAATTY 2 cut(s) 159, 500
AseI ATTAAT 1 cut(s) 336
AsiGI ACCGGT 1 cut(s) 307
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 3 cut(s) 162, 413, 610
BbsI GAAGAC 2 cut(s) 96, 159
Bbv12I GWGCWC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 4 cut(s) 273, 310, 338, 431
BccI CCATC 2 cut(s) 104, 401
BciVI GTATCC 1 cut(s) 473
BclI TGATCA 2 cut(s) 40, 78
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 3 cut(s) 563, 720, 734
BfaI CTAG 4 cut(s) 462, 719, 794, 799
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 586
BfuI GTATCC 1 cut(s) 473
BisI GCNGC 6 cut(s) 123, 287, 290, 324, 327, 420
BlsI GCNGC 6 cut(s) 124, 288, 291, 325, 328, 421
Bme1390I CCNGG 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 2 cut(s) 96, 159
BplI GAGNNNNNCTC 2 cut(s) 543, 575
BpmI CTGGAG 1 cut(s) 415
Bpu10I CCTNAGC 2 cut(s) 492, 543
BpuEI CTTGAG 2 cut(s) 77, 501
BpuMI CCSGG 1 cut(s) 399
BsaHI GRCGYC 2 cut(s) 510, 790
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 2 cut(s) 204, 397
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bse118I RCCGGY 2 cut(s) 224, 307
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 204, 397
BseGI GGATG 2 cut(s) 316, 769
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 3 cut(s) 233, 483, 557
BseNI ACTGG 1 cut(s) 432
BseXI GCAGC 4 cut(s) 273, 310, 338, 431
Bsh1236I CGCG 1 cut(s) 540
BshFI GGCC 2 cut(s) 209, 224
BshTI ACCGGT 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 3 cut(s) 225, 308, 399
BslFI GGGAC 2 cut(s) 380, 521
BsmAI GTCTC 3 cut(s) 563, 720, 734
BsmBI CGTCTC 2 cut(s) 720, 734
BsmFI GGGAC 2 cut(s) 380, 521
BsnI GGCC 2 cut(s) 209, 224
Bso31I GGTCTC 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 6 cut(s) 40, 78, 301, 436, 627, 709
BspACI CCGC 2 cut(s) 123, 290
BspANI GGCC 2 cut(s) 209, 224
BspCNI CTCAG 3 cut(s) 232, 484, 556
BspFNI CGCG 1 cut(s) 540
BspHI TCATGA 1 cut(s) 561
BspMAI CTGCAG 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 296, 704
BspQI GCTCTTC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 281
BsrFI RCCGGY 2 cut(s) 224, 307
BsrI ACTGG 1 cut(s) 432
BssAI RCCGGY 2 cut(s) 224, 307
BssECI CCNNGG 2 cut(s) 204, 397
BssMI GATC 6 cut(s) 40, 78, 301, 436, 627, 709
BssNI GRCGYC 2 cut(s) 510, 790
Bst4CI ACNGT 2 cut(s) 668, 688
Bst6I CTCTTC 2 cut(s) 585, 599
BstACI GRCGYC 2 cut(s) 510, 790
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 31, 531
BstDEI CTNAG 4 cut(s) 219, 492, 543, 785
BstF5I GGATG 2 cut(s) 316, 769
BstFNI CGCG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 6 cut(s) 43, 81, 304, 439, 630, 712
BstMAI GTCTC 3 cut(s) 563, 720, 734
BstMBI GATC 6 cut(s) 40, 78, 301, 436, 627, 709
BstMWI GCNNNNNNNGC 2 cut(s) 425, 539
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 4 cut(s) 273, 310, 338, 431
BstV2I GAAGAC 2 cut(s) 96, 159
BsuI GTATCC 1 cut(s) 473
BsuRI GGCC 2 cut(s) 209, 224
BtsCI GGATG 2 cut(s) 316, 769
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 3 cut(s) 439, 630, 673
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 31, 531
CaiI CAGNNNCTG 2 cut(s) 49, 425
CciI TCATGA 1 cut(s) 561
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 2 cut(s) 224, 307
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 2 cut(s) 61, 518
Csp6I GTAC 1 cut(s) 778
CspAI ACCGGT 1 cut(s) 307
CspCI CAANNNNNGTGG 4 cut(s) 264, 294, 299, 329
CviAII CATG 1 cut(s) 562
CviJI RGCY 8 cut(s) 84, 109, 122, 209, 224, 329, 529, 648
CviKI_1 RGCY 8 cut(s) 84, 109, 122, 209, 224, 329, 529, 648
CviQI GTAC 1 cut(s) 778
DdeI CTNAG 4 cut(s) 219, 492, 543, 785
DpnI GATC 6 cut(s) 42, 80, 303, 438, 629, 711
DpnII GATC 6 cut(s) 40, 78, 301, 436, 627, 709
Eam1104I CTCTTC 2 cut(s) 585, 599
EarI CTCTTC 2 cut(s) 585, 599
Eco31I GGTCTC 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 208
Esp3I CGTCTC 2 cut(s) 720, 734
FaeI CATG 1 cut(s) 565
FaiI YATR 9 cut(s) 234, 362, 364, 417, 477, 486, 525, 563, 735
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 2 cut(s) 380, 521
FatI CATG 1 cut(s) 561
FbaI TGATCA 2 cut(s) 40, 78
Fnu4HI GCNGC 6 cut(s) 123, 287, 290, 324, 327, 420
FokI GGATG 2 cut(s) 303, 776
Fsp4HI GCNGC 6 cut(s) 123, 287, 290, 324, 327, 420
FspBI CTAG 4 cut(s) 462, 719, 794, 799
GlaI GCGC 1 cut(s) 541
GluI GCNGC 6 cut(s) 123, 287, 290, 324, 327, 420
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 2 cut(s) 209, 224
HapII CCGG 3 cut(s) 225, 308, 399
HgaI GACGC 2 cut(s) 61, 518
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 2 cut(s) 510, 790
Hin1II CATG 1 cut(s) 565
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HinfI GANTC 1 cut(s) 469
HpaII CCGG 3 cut(s) 225, 308, 399
HphI GGTGA 3 cut(s) 162, 413, 610
Hpy166II GTNNAC 2 cut(s) 449, 573
Hpy188III TCNNGA 6 cut(s) 196, 440, 462, 518, 545, 562
Hpy8I GTNNAC 2 cut(s) 449, 573
HpyAV CCTTC 7 cut(s) 55, 136, 183, 221, 288, 459, 548
HpyCH4III ACNGT 2 cut(s) 668, 688
HpyCH4V TGCA 4 cut(s) 316, 326, 419, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 539
HpyF3I CTNAG 4 cut(s) 219, 492, 543, 785
Hsp92I GRCGYC 2 cut(s) 510, 790
Hsp92II CATG 1 cut(s) 565
HspAI GCGC 1 cut(s) 540
Ksp22I TGATCA 2 cut(s) 40, 78
Kzo9I GATC 6 cut(s) 40, 78, 301, 436, 627, 709
LguI GCTCTTC 1 cut(s) 585
Lsp1109I GCAGC 4 cut(s) 273, 310, 338, 431
LweI GCATC 1 cut(s) 325
MaeI CTAG 4 cut(s) 462, 719, 794, 799
MalI GATC 6 cut(s) 42, 80, 303, 438, 629, 711
MboI GATC 6 cut(s) 40, 78, 301, 436, 627, 709
MboII GAAGA 7 cut(s) 101, 159, 257, 602, 613, 616, 628
MfeI CAATTG 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 5 cut(s) 159, 374, 500, 534, 566
MnlI CCTC 9 cut(s) 141, 199, 303, 393, 439, 487, 525, 762, 791
MseI TTAA 3 cut(s) 183, 336, 342
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 3 cut(s) 225, 308, 399
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 374
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 2 cut(s) 425, 539
NciI CCSGG 1 cut(s) 399
NdeII GATC 6 cut(s) 40, 78, 301, 436, 627, 709
NlaIII CATG 1 cut(s) 565
NmeAIII GCCGAG 1 cut(s) 5
OliI CACNNNNGTG 1 cut(s) 98
PagI TCATGA 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 469
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 6 cut(s) 124, 288, 291, 325, 328, 421
PshBI ATTAAT 1 cut(s) 336
PspPI GGNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 49, 425
RsaI GTAC 1 cut(s) 779
RsaNI GTAC 1 cut(s) 778
RseI CAYNNNNRTG 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 3 cut(s) 183, 336, 342
SatI GCNGC 6 cut(s) 123, 287, 290, 324, 327, 420
Sau3AI GATC 6 cut(s) 40, 78, 301, 436, 627, 709
Sau96I GGNCC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 56, 516
SmoI CTYRAG 2 cut(s) 56, 516
Sse9I AATT 5 cut(s) 159, 374, 500, 534, 566
SsiI CCGC 2 cut(s) 123, 290
SspMI CTAG 4 cut(s) 462, 719, 794, 799
StyD4I CCNGG 1 cut(s) 397
TaaI ACNGT 2 cut(s) 668, 688
TasI AATT 5 cut(s) 159, 374, 500, 534, 566
TauI GCSGC 2 cut(s) 125, 292
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 3 cut(s) 183, 336, 342
Tru9I TTAA 3 cut(s) 183, 336, 342
TscAI CASTG 3 cut(s) 439, 630, 673
TseI GCWGC 4 cut(s) 286, 323, 326, 419
TspDTI ATGAA 5 cut(s) 178, 249, 270, 461, 578
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 3 cut(s) 439, 630, 673
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 2 cut(s) 159, 500
XbaI TCTAGA 1 cut(s) 461
XspI CTAG 4 cut(s) 462, 719, 794, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.