Rh6DG519000

Transcription factor S-II (TFIIS), central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002519
Physical Location & Seq
Reverse (-)
66965096 .. 66967068
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG519000.1

Sequence Viewer

Length: 1242 bp
ATGTACAGAAATGTATGTTACCTCATGTATTGCCAAATAAAGTGTTTCTATTCATCTAATTTATTTATTTTTCTTGTTTTTTATTTCAGCGGTGAAAAAACCTCCGCAAAAGACTGGCCTGGGTCTCTCGAGATCAAGGGCAGAGTCAGGCTCGATGCTTTTGAGAAATTCCTGCAAGAGCTTCCACAATCCCGAAGTCGTGCTGTTATGGTTGTGCATTTTGTTCTTAAAGAGGGGTCGTCAGAGACTGAATCTGCAAGCCTTCGTGAGGTCCGAGATTCTTATATTTCAGATGAGAGAGTGGGTTTTGCTGAGCCTTGTTCTGGTGTTGAACTATATTTTTGCCCTCCCCATAATAAAACTTGTGACATGCTCAGCAAGATCATTCAGAAGGAACACGTTGAGGCACTGAACACTATTGATAATGGCCTTATTGGTGTTATTGTGTGGAGAAAGTTAACTTCCCCTAAATCATCTTCGCACCAGAAACACGCTTCAAAAAAGCAACACTATAGTAGTTCTACTTCTAGGAGGCATCATGATACAAATTCGAATGCCAATTACAATTCCAAACCTTCCCAGCCTAGGGTCTTACCTCCCACCCATACTAAAGTAACACATGATGATGAGGAGGACGAAGTTCCTCCTGGATTTGGCCCTCCGGCTTCCCGGGACGATGATGACCTTCCTGAATTCAATTATAGTGGTGCGTCAAATCCGTCTCTGCCAAAGTTTTCTACCCAAAGGTCCTCTAGGGGGCCTGGGGTGTACCCCGAGTCTCAGACTCCTCGCCCTGTAGATAAAATGAGAGAGCTGATTCTTAAATATGGACAAAATGACAGCCGTGCATCATGGAATGATGATGATGATGATGATGATGATGACATCCCAGAGTGGCAGCCAAATCCAACCCCATCCCAGTACCAGCGGCCACAGCTACAACCAGTGAACAACTACCAGCAACCAATGCTGCGAGCCCGTATCGGTTCTCCACTTCAGCAGCAACCATTGCAAACCCCGCAACCCCAAATGCATGTGGCATCAGGCTTACAGACTCCTAGTCCTTATTGGCAGCAAGGGAATCAGTGGGCTCCTCCTCCTCCACAGAGTGGCGGCGTGTGGGCGACTAATGTAGCTTGTCAGCCAGAATCAGGGCAGTTTTATGGAGAACCTGATAGAGGAACAGCAGGTCAGCCGGGAATAGCCTGGCGACAGAACGCTCCTAGAAGTAGAGGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPOC TFIIS_M

Protein Analysis

413

Amino Acids

46.69

Weight (kDa)

6.28

Isoelectric Point (pI)

60.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 26 - 151 5.5e-25 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1178
AccB7I CCANNNNNTGG 1 cut(s) 1109
AciI CCGC 5 cut(s) 90, 105, 928, 1019, 1113
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 3 cut(s) 167, 547, 692
AcuI CTGAAG 1 cut(s) 980
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 3 cut(s) 5, 770, 923
AfiI CCNNNNNNNGG 9 cut(s) 268, 323, 585, 586, 653, 756, 1109, 1151, 1178
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 3 cut(s) 332, 498, 697
AhdI GACNNNNNGTC 1 cut(s) 1059
AjnI CCWGG 4 cut(s) 118, 646, 760, 1205
AluBI AGCT 4 cut(s) 181, 814, 937, 1136
AluI AGCT 4 cut(s) 181, 814, 937, 1136
Alw26I GTCTC 4 cut(s) 129, 239, 726, 783
AlwNI CAGNNNCTG 1 cut(s) 248
Ama87I CYCGRG 3 cut(s) 128, 669, 773
AoxI GGCC 5 cut(s) 116, 427, 655, 758, 929
ApeKI GCWGC 4 cut(s) 898, 970, 1000, 1072
ApoI RAATTY 3 cut(s) 167, 547, 692
AspA2I CCTAGG 1 cut(s) 584
AspS9I GGNCC 4 cut(s) 271, 656, 747, 758
AsuC2I CCSGG 3 cut(s) 670, 671, 1197
AsuHPI GGTGA 1 cut(s) 104
AsuII TTCGAA 1 cut(s) 551
AvaI CYCGRG 3 cut(s) 128, 669, 773
AvaII GGWCC 2 cut(s) 271, 747
AvrII CCTAGG 1 cut(s) 584
BanII GRGCYC 2 cut(s) 979, 1093
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
BbvI GCAGC 4 cut(s) 910, 957, 1012, 1084
BccI CCATC 1 cut(s) 922
BceAI ACGGC 1 cut(s) 828
BciT130I CCWGG 4 cut(s) 120, 648, 762, 1207
BcnI CCSGG 3 cut(s) 670, 671, 1197
BcoDI GTCTC 4 cut(s) 129, 239, 726, 783
BfaI CTAG 5 cut(s) 528, 585, 753, 1059, 1224
BfmI CTRYAG 2 cut(s) 511, 795
BfuAI ACCTGC 1 cut(s) 1178
BisI GCNGC 6 cut(s) 899, 929, 971, 1001, 1073, 1114
BlnI CCTAGG 1 cut(s) 584
BlpI GCTNAGC 2 cut(s) 312, 374
BlsI GCNGC 6 cut(s) 900, 930, 972, 1002, 1074, 1115
Bme1390I CCNGG 7 cut(s) 120, 648, 670, 671, 762, 1197, 1207
Bme18I GGWCC 2 cut(s) 271, 747
BmeRI GACNNNNNGTC 1 cut(s) 1059
BmeT110I CYCGRG 3 cut(s) 128, 669, 773
BmgT120I GGNCC 4 cut(s) 271, 656, 747, 758
BmiI GGNNCC 2 cut(s) 759, 1092
BmrFI CCNGG 7 cut(s) 120, 648, 670, 671, 762, 1197, 1207
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 4 cut(s) 145, 544, 857, 1049
BmuI ACTGGG 1 cut(s) 913
BplI GAGNNNNNCTC 2 cut(s) 135, 167
Bpu1102I GCTNAGC 2 cut(s) 312, 374
Bpu14I TTCGAA 1 cut(s) 551
BpuMI CCSGG 3 cut(s) 670, 671, 1197
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 119, 584, 669, 761
Bsc4I CCNNNNNNNGG 9 cut(s) 268, 323, 585, 586, 653, 756, 1109, 1151, 1178
Bse1I ACTGG 3 cut(s) 119, 919, 944
Bse3DI GCAATG 1 cut(s) 1007
BseBI CCWGG 4 cut(s) 120, 648, 762, 1207
BseDI CCNNGG 4 cut(s) 119, 584, 669, 761
BseGI GGATG 2 cut(s) 885, 914
BseLI CCNNNNNNNGG 9 cut(s) 268, 323, 585, 586, 653, 756, 1109, 1151, 1178
BseMI GCAATG 1 cut(s) 1007
BseMII CTCAG 3 cut(s) 303, 388, 794
BseNI ACTGG 3 cut(s) 119, 919, 944
BseRI GAGGAG 5 cut(s) 644, 777, 1083, 1086, 1089
BseXI GCAGC 4 cut(s) 910, 957, 1012, 1084
BseYI CCCAGC 1 cut(s) 579
BshFI GGCC 5 cut(s) 118, 429, 657, 760, 931
BsiHKCI CYCGRG 3 cut(s) 128, 669, 773
BsiSI CCGG 3 cut(s) 662, 670, 1196
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 9 cut(s) 268, 323, 585, 586, 653, 756, 1109, 1151, 1178
BsmAI GTCTC 4 cut(s) 129, 239, 726, 783
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 5 cut(s) 118, 429, 657, 760, 931
Bso31I GGTCTC 1 cut(s) 129
BsoBI CYCGRG 3 cut(s) 128, 669, 773
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 979, 1093
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 132, 381
Bsp1720I GCTNAGC 2 cut(s) 312, 374
BspACI CCGC 5 cut(s) 90, 105, 928, 1019, 1113
BspANI GGCC 5 cut(s) 118, 429, 657, 760, 931
BspCNI CTCAG 3 cut(s) 304, 387, 793
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 759, 1092
BspMI ACCTGC 1 cut(s) 1178
BspT104I TTCGAA 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 1007
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 119, 919, 944
BssECI CCNNGG 4 cut(s) 119, 584, 669, 761
BssMI GATC 2 cut(s) 132, 381
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 4 cut(s) 120, 648, 762, 1207
BstAPI GCANNNNNTGC 2 cut(s) 967, 1009
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 259, 975
BstDEI CTNAG 3 cut(s) 312, 374, 780
BstENI CCTNNNNNAGG 2 cut(s) 266, 1176
BstF5I GGATG 2 cut(s) 885, 914
BstKTI GATC 2 cut(s) 135, 384
BstMAI GTCTC 4 cut(s) 129, 239, 726, 783
BstMBI GATC 2 cut(s) 132, 381
BstMWI GCNNNNNNNGC 4 cut(s) 934, 967, 1009, 1018
BstNI CCWGG 4 cut(s) 120, 648, 762, 1207
BstNSI RCATGY 2 cut(s) 373, 1037
BstSCI CCNGG 7 cut(s) 118, 646, 668, 669, 760, 1195, 1205
BstSFI CTRYAG 2 cut(s) 511, 795
BstV1I GCAGC 4 cut(s) 910, 957, 1012, 1084
BsuRI GGCC 5 cut(s) 118, 429, 657, 760, 931
BtsCI GGATG 2 cut(s) 885, 914
BtsIMutI CAGTG 3 cut(s) 407, 951, 1091
BveI ACCTGC 1 cut(s) 1178
Cac8I GCNNGC 2 cut(s) 259, 975
CaiI CAGNNNCTG 1 cut(s) 248
CciI TCATGA 1 cut(s) 538
Cfr13I GGNCC 4 cut(s) 271, 656, 747, 758
Cfr9I CCCGGG 1 cut(s) 669
CseI GACGC 1 cut(s) 699
Csp6I GTAC 3 cut(s) 4, 769, 922
CspCI CAANNNNNGTGG 2 cut(s) 685, 720
CviAII CATG 6 cut(s) 25, 370, 539, 620, 852, 1034
CviQI GTAC 3 cut(s) 4, 769, 922
DdeI CTNAG 3 cut(s) 312, 374, 780
DpnI GATC 2 cut(s) 134, 383
DpnII GATC 2 cut(s) 132, 381
DraIII CACNNNGTG 1 cut(s) 1109
DriI GACNNNNNGTC 1 cut(s) 1059
EaeI YGGCCR 1 cut(s) 929
Eam1105I GACNNNNNGTC 1 cut(s) 1059
Eco130I CCWWGG 1 cut(s) 584
Eco24I GRGCYC 2 cut(s) 979, 1093
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 271, 747
Eco57I CTGAAG 1 cut(s) 980
Eco88I CYCGRG 3 cut(s) 128, 669, 773
EcoNI CCTNNNNNAGG 2 cut(s) 266, 1176
EcoO109I RGGNCCY 2 cut(s) 747, 758
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 4 cut(s) 118, 646, 760, 1205
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 2 cut(s) 979, 1093
ErhI CCWWGG 1 cut(s) 584
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 6 cut(s) 28, 373, 542, 623, 855, 1037
FaqI GGGAC 1 cut(s) 686
FatI CATG 6 cut(s) 24, 369, 538, 619, 851, 1033
FauI CCCGC 1 cut(s) 1026
Fnu4HI GCNGC 6 cut(s) 899, 929, 971, 1001, 1073, 1114
FokI GGATG 2 cut(s) 872, 901
FriOI GRGCYC 2 cut(s) 979, 1093
Fsp4HI GCNGC 6 cut(s) 899, 929, 971, 1001, 1073, 1114
FspBI CTAG 5 cut(s) 528, 585, 753, 1059, 1224
GluI GCNGC 6 cut(s) 899, 929, 971, 1001, 1073, 1114
GsaI CCCAGC 1 cut(s) 583
HaeIII GGCC 5 cut(s) 118, 429, 657, 760, 931
HapII CCGG 3 cut(s) 662, 670, 1196
HgaI GACGC 1 cut(s) 699
Hin1II CATG 6 cut(s) 28, 373, 542, 623, 855, 1037
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 9 cut(s) 144, 251, 278, 776, 784, 817, 1054, 1081, 1148
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 3 cut(s) 662, 670, 1196
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 3 cut(s) 459, 769, 949
Hpy188I TCNGA 5 cut(s) 244, 275, 292, 390, 783
Hpy188III TCNNGA 6 cut(s) 128, 130, 192, 266, 539, 689
Hpy8I GTNNAC 3 cut(s) 459, 769, 949
HpyAV CCTTC 4 cut(s) 272, 385, 585, 695
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 6 cut(s) 175, 217, 257, 848, 1012, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 934, 967, 1009, 1018
HpyF3I CTNAG 3 cut(s) 312, 374, 780
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 6 cut(s) 28, 373, 542, 623, 855, 1037
KspAI GTTAAC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 132, 381
LmnI GCTCC 2 cut(s) 1096, 1225
Lsp1109I GCAGC 4 cut(s) 910, 957, 1012, 1084
LweI GCATC 4 cut(s) 145, 544, 857, 1049
MaeI CTAG 5 cut(s) 528, 585, 753, 1059, 1224
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 3 cut(s) 17, 365, 613
MalI GATC 2 cut(s) 134, 383
MboI GATC 2 cut(s) 132, 381
MboII GAAGA 1 cut(s) 468
MhlI GDGCHC 2 cut(s) 979, 1093
MluCI AATT 7 cut(s) 58, 167, 547, 559, 565, 692, 697
MlyI GAGTC 4 cut(s) 153, 778, 785, 1048
MmeI TCCRAC 1 cut(s) 932
Mph1103I ATGCAT 1 cut(s) 1035
MseI TTAA 4 cut(s) 228, 458, 822, 1240
MslI CAYNNNNRTG 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 90, 928
MspI CCGG 3 cut(s) 662, 670, 1196
MspR9I CCNGG 7 cut(s) 120, 648, 670, 671, 762, 1197, 1207
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 4 cut(s) 120, 648, 762, 1207
MwoI GCNNNNNNNGC 4 cut(s) 934, 967, 1009, 1018
NciI CCSGG 3 cut(s) 670, 671, 1197
NdeII GATC 2 cut(s) 132, 381
NlaIII CATG 6 cut(s) 28, 373, 542, 623, 855, 1037
NlaIV GGNNCC 2 cut(s) 759, 1092
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 2 cut(s) 373, 1037
NspV TTCGAA 1 cut(s) 551
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 5 cut(s) 251, 278, 817, 1081, 1148
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 646
PkrI GCNGC 6 cut(s) 900, 930, 972, 1002, 1074, 1115
PleI GAGTC 4 cut(s) 152, 778, 784, 1048
PpsI GAGTC 4 cut(s) 152, 778, 784, 1048
PpuMI RGGWCCY 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 747
Psp6I CCWGG 4 cut(s) 118, 646, 760, 1205
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 4 cut(s) 118, 646, 760, 1205
PspN4I GGNNCC 2 cut(s) 759, 1092
PspPI GGNCC 4 cut(s) 271, 656, 747, 758
PspPPI RGGWCCY 1 cut(s) 747
PstNI CAGNNNCTG 1 cut(s) 248
RsaI GTAC 3 cut(s) 5, 770, 923
RsaNI GTAC 3 cut(s) 4, 769, 922
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 4 cut(s) 228, 458, 822, 1240
SatI GCNGC 6 cut(s) 899, 929, 971, 1001, 1073, 1114
Sau3AI GATC 2 cut(s) 132, 381
Sau96I GGNCC 4 cut(s) 271, 656, 747, 758
SchI GAGTC 4 cut(s) 153, 778, 785, 1048
ScrFI CCNGG 7 cut(s) 120, 648, 670, 671, 762, 1197, 1207
SduI GDGCHC 2 cut(s) 979, 1093
SfaNI GCATC 4 cut(s) 145, 544, 857, 1049
SfcI CTRYAG 2 cut(s) 511, 795
Sfr274I CTCGAG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 2 cut(s) 271, 747
SlaI CTCGAG 1 cut(s) 128
SmaI CCCGGG 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 624
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 7 cut(s) 58, 167, 547, 559, 565, 692, 697
SsiI CCGC 5 cut(s) 90, 105, 928, 1019, 1113
SspMI CTAG 5 cut(s) 528, 585, 753, 1059, 1224
StyD4I CCNGG 7 cut(s) 118, 646, 668, 669, 760, 1195, 1205
StyI CCWWGG 1 cut(s) 584
TaiI ACGT 1 cut(s) 402
TaqI TCGA 3 cut(s) 129, 153, 551
TasI AATT 7 cut(s) 58, 167, 547, 559, 565, 692, 697
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 931, 1116
TfiI GAWTC 5 cut(s) 251, 278, 817, 1081, 1148
Tru1I TTAA 4 cut(s) 228, 458, 822, 1240
Tru9I TTAA 4 cut(s) 228, 458, 822, 1240
TscAI CASTG 3 cut(s) 414, 951, 1091
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 4 cut(s) 898, 970, 1000, 1072
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 1 cut(s) 708
TspMI CCCGGG 1 cut(s) 669
TspRI CASTG 3 cut(s) 414, 951, 1091
Van91I CCANNNNNTGG 1 cut(s) 1109
VpaK11BI GGWCC 2 cut(s) 271, 747
XagI CCTNNNNNAGG 2 cut(s) 266, 1176
XapI RAATTY 3 cut(s) 167, 547, 692
XceI RCATGY 2 cut(s) 373, 1037
XhoI CTCGAG 1 cut(s) 128
XmaI CCCGGG 1 cut(s) 669
XmaJI CCTAGG 1 cut(s) 584
XspI CTAG 5 cut(s) 528, 585, 753, 1059, 1224
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.