Rh6BG528200

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009835
Physical Location & Seq
Reverse (-)
71908396 .. 71912290
3895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG528200.1

Sequence Viewer

Length: 1293 bp
ATGGATGTTGGCTCATTGAAGCTTCAACTTGAAAGTCAAACTTACACGACCCCATTGTTAATGGAGCAATCTCAACCAGAAAATATTGCAAGCAACGAGCACATTATTGACATAGCTGGGAGTGAGCATGCCTCCTCATCTAGCTTGCCTCATGGTAGATCTTTAAATGGCCTAAATGCCACACAGCAAGACAGACCTTCAAGTAGTCCACGGGCACCCGTATCCCAACCTTCAATCTTTTCTTCTAATGGATCAAACTCTAGAAACACTTCAGTGGCAAGGAGAGGAGAGACTCGTCGCCGCAGAAGTCCGTTGAATTCTGGAATATGGATATCTATTGAACTGGTTCTTACTGTGAGCCAAATCGTTGCATCTATTGTCGTTTTGTCTTTATCTAGGAATGAACATCCACGCACTCCATTGTTTGCATGGATTGTTGGTTATGCATCTGGATGTGTTGCTACTCTTCCTCTTCTTTATTGGCGCTATCGTCATCGCAACCAGGTCTCAGAACAAGATTCTGTTCAAGCTCGTCAGAATTCTCAGCTATCGGCAAGACCATTTTCACTCTCTCTATCTAGGGCTTCAGAAGGAGAAGATCTTCAGACTGCCACTGCAACCCATAGAAGCAATCAAAGTTCGGCAGTACTAAGCAGAAGACTCAAAGTACTTGTGGAATACTTCAAGATGGCGTTGGATTGTTTCTTTGCGGTTTGGTTCGTGGTTGGCAATGTTTGGATATTTGGAGGACACTCATCTGCGAATGATGCACCTAACCAGTACAGGTTATGTATAGTATTTCTGACATTTAGCTGTATCGGATACGCCATGCCTTTCATCTTATGTGCAACAATCTGCTGCTGTCTTCCCTGTATAATATCAGTTCTTGGGTTTCGAGAAGATCTGACACAGGCACGAGGGGCAACGCCTGAATCAATTAATGCTCTCCCAACATACAAATTCAAGTTGAAGAAAAACAGAAATGGTGATAACAGTGAAGGTGTTGTGGCTGCAGGAACTGAGAAGGAGCGGGTGATTTCTGGAGAGGATGCGGTTTGTTGCATCTGCTTGGCAAAGTATGCAAACAATGACGAGCTGAGAGAGTTGCCGTGTTCTCATTTTTTCCACAAGGATTGTGTGGATAAGTGGTTGAAGATCAATGCGTCTTGTCCTCTTTGCAAGAGTGAGGTTGGTGAGAGCCTTTTAGGTTCTCTCTCCTCAATCAATGGCAGTCAGCAACGGAGTGATAGCAGGGTGGGCAATGAAGTGGCTGAGGTCAACACTGTGTTTTAG

Protein Analysis

430

Amino Acids

47.25

Weight (kDa)

8.3

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 353 - 394 1.4e-14 Ring finger domain
zf-RING_11 PF17123 353 - 380 5.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 353 - 393 5e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 353 - 393 5.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 355 - 394 1.5e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 1030
AciI CCGC 4 cut(s) 301, 710, 1030, 1052
AclWI GGATC 1 cut(s) 259
AcsI RAATTY 3 cut(s) 316, 538, 959
AcuI CTGAAG 3 cut(s) 255, 570, 587
AfaI GTAC 3 cut(s) 648, 669, 782
AjnI CCWGG 1 cut(s) 501
AleI CACNNNNGTG 1 cut(s) 272
AluBI AGCT 7 cut(s) 22, 116, 144, 530, 547, 813, 1096
AluI AGCT 7 cut(s) 22, 116, 144, 530, 547, 813, 1096
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 284, 511
AlwI GGATC 1 cut(s) 259
AlwNI CAGNNNCTG 1 cut(s) 1019
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 2 cut(s) 858, 1010
ApoI RAATTY 3 cut(s) 316, 538, 959
AseI ATTAAT 1 cut(s) 939
Asp700I GAANNNNTTC 3 cut(s) 268, 345, 600
AspLEI GCGC 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 998, 1045, 1205
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 214
BauI CACGAG 1 cut(s) 915
BbsI GAAGAC 2 cut(s) 664, 857
Bbv12I GWGCWC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 1272
BbvI GCAGC 2 cut(s) 845, 997
BccI CCATC 1 cut(s) 682
BceAI ACGGC 1 cut(s) 1093
BciT130I CCWGG 1 cut(s) 503
BciVI GTATCC 2 cut(s) 232, 815
BcoDI GTCTC 2 cut(s) 284, 511
BfaI CTAG 4 cut(s) 141, 261, 396, 579
BfmI CTRYAG 1 cut(s) 1011
BfoI RGCGCY 1 cut(s) 487
BfuI GTATCC 2 cut(s) 232, 815
BglII AGATCT 3 cut(s) 158, 598, 901
BisI GCNGC 3 cut(s) 301, 859, 1011
BlsI GCNGC 3 cut(s) 302, 860, 1012
BmcAI AGTACT 2 cut(s) 648, 669
Bme1390I CCNGG 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 5 cut(s) 380, 455, 757, 1039, 1071
BpiI GAAGAC 2 cut(s) 664, 857
BplI GAGNNNNNCTC 2 cut(s) 116, 148
BpmI CTGGAG 1 cut(s) 1062
Bpu10I CCTNAGC 1 cut(s) 1272
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 1 cut(s) 209
Bse1I ACTGG 2 cut(s) 348, 778
Bse3DI GCAATG 2 cut(s) 736, 1267
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 4 cut(s) 10, 406, 458, 1054
BseMI GCAATG 2 cut(s) 736, 1267
BseMII CTCAG 5 cut(s) 522, 557, 1011, 1088, 1263
BseNI ACTGG 2 cut(s) 348, 778
BseRI GAGGAG 3 cut(s) 124, 300, 1207
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 845, 997
BseYI CCCAGC 1 cut(s) 116
BshFI GGCC 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 284, 511
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 1 cut(s) 511
Bsp1286I GDGCHC 2 cut(s) 102, 217
Bsp143I GATC 5 cut(s) 158, 251, 598, 901, 1155
BspACI CCGC 4 cut(s) 301, 710, 1030, 1052
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 5 cut(s) 521, 556, 1012, 1089, 1264
BspLI GGNNCC 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 511
BsrBI CCGCTC 1 cut(s) 1030
BsrDI GCAATG 2 cut(s) 736, 1267
BsrI ACTGG 2 cut(s) 348, 778
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 5 cut(s) 158, 251, 598, 901, 1155
BssSI CACGAG 1 cut(s) 915
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 3 cut(s) 355, 995, 1285
Bst6I CTCTTC 2 cut(s) 471, 477
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstC8I GCNNGC 3 cut(s) 91, 129, 146
BstDEI CTNAG 6 cut(s) 508, 543, 650, 1020, 1097, 1272
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 10, 406, 458, 1054
BstH2I RGCGCY 1 cut(s) 487
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 5 cut(s) 161, 254, 601, 904, 1158
BstMAI GTCTC 2 cut(s) 284, 511
BstMBI GATC 5 cut(s) 158, 251, 598, 901, 1155
BstMWI GCNNNNNNNGC 4 cut(s) 767, 920, 1079, 1257
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 845, 997
BstV2I GAAGAC 2 cut(s) 664, 857
BstX2I RGATCY 3 cut(s) 158, 598, 901
BstYI RGATCY 3 cut(s) 158, 598, 901
BsuI GTATCC 2 cut(s) 232, 815
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 209
BtgZI GCGATG 1 cut(s) 479
BtsCI GGATG 4 cut(s) 10, 406, 458, 1054
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 4 cut(s) 279, 612, 1000, 1281
Cac8I GCNNGC 3 cut(s) 91, 129, 146
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 1 cut(s) 486
CseI GACGC 1 cut(s) 1152
CsiI ACCWGGT 1 cut(s) 501
Csp6I GTAC 3 cut(s) 647, 668, 781
CspCI CAANNNNNGTGG 2 cut(s) 1115, 1150
CviAII CATG 4 cut(s) 128, 152, 429, 829
CviQI GTAC 3 cut(s) 647, 668, 781
DdeI CTNAG 6 cut(s) 508, 543, 650, 1020, 1097, 1272
DpnI GATC 5 cut(s) 160, 253, 600, 903, 1157
DpnII GATC 5 cut(s) 158, 251, 598, 901, 1155
DraI TTTAAA 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 471, 477
EarI CTCTTC 2 cut(s) 471, 477
Eco31I GGTCTC 1 cut(s) 511
Eco32I GATATC 1 cut(s) 333
Eco57I CTGAAG 3 cut(s) 255, 570, 587
EcoRI GAATTC 2 cut(s) 316, 538
EcoRII CCWGG 1 cut(s) 501
EcoRV GATATC 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 448
FaeI CATG 4 cut(s) 131, 155, 432, 832
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FatI CATG 4 cut(s) 127, 151, 428, 828
FauI CCCGC 1 cut(s) 1023
Fnu4HI GCNGC 3 cut(s) 301, 859, 1011
FokI GGATG 4 cut(s) 17, 393, 465, 1061
Fsp4HI GCNGC 3 cut(s) 301, 859, 1011
FspBI CTAG 4 cut(s) 141, 261, 396, 579
GlaI GCGC 1 cut(s) 485
GluI GCNGC 3 cut(s) 301, 859, 1011
GsaI CCCAGC 1 cut(s) 120
GsuI CTGGAG 1 cut(s) 1062
HaeII RGCGCY 1 cut(s) 487
HaeIII GGCC 1 cut(s) 171
HgaI GACGC 1 cut(s) 1152
HhaI GCGC 1 cut(s) 486
Hin1II CATG 4 cut(s) 131, 155, 432, 832
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HincII GTYRAC 1 cut(s) 1279
HindII GTYRAC 1 cut(s) 1279
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 4 cut(s) 292, 518, 660, 932
HphI GGTGA 3 cut(s) 998, 1045, 1205
Hpy166II GTNNAC 2 cut(s) 209, 1279
Hpy188I TCNGA 7 cut(s) 511, 537, 589, 606, 804, 821, 906
Hpy188III TCNNGA 6 cut(s) 261, 321, 450, 685, 896, 1041
Hpy8I GTNNAC 2 cut(s) 209, 1279
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 5 cut(s) 207, 240, 584, 992, 1018
HpyCH4III ACNGT 3 cut(s) 355, 995, 1285
HpyF10VI GCNNNNNNNGC 4 cut(s) 767, 920, 1079, 1257
HpyF3I CTNAG 6 cut(s) 508, 543, 650, 1020, 1097, 1272
Hsp92II CATG 4 cut(s) 131, 155, 432, 832
HspAI GCGC 1 cut(s) 484
Kzo9I GATC 5 cut(s) 158, 251, 598, 901, 1155
LmnI GCTCC 2 cut(s) 64, 1027
Lsp1109I GCAGC 2 cut(s) 845, 997
LweI GCATC 5 cut(s) 380, 455, 757, 1039, 1071
MabI ACCWGGT 1 cut(s) 501
MaeI CTAG 4 cut(s) 141, 261, 396, 579
MalI GATC 5 cut(s) 160, 253, 600, 903, 1157
MbiI CCGCTC 1 cut(s) 1030
MboI GATC 5 cut(s) 158, 251, 598, 901, 1155
MflI RGATCY 3 cut(s) 158, 598, 901
MhlI GDGCHC 2 cut(s) 102, 217
MluCI AATT 4 cut(s) 316, 538, 936, 959
MlyI GAGTC 2 cut(s) 286, 654
MmeI TCCRAC 1 cut(s) 675
Mph1103I ATGCAT 1 cut(s) 448
MroXI GAANNNNTTC 3 cut(s) 268, 345, 600
MseI TTAA 3 cut(s) 59, 164, 939
MslI CAYNNNNRTG 2 cut(s) 272, 451
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MwoI GCNNNNNNNGC 4 cut(s) 767, 920, 1079, 1257
NdeII GATC 5 cut(s) 158, 251, 598, 901, 1155
NlaIII CATG 4 cut(s) 131, 155, 432, 832
NlaIV GGNNCC 1 cut(s) 216
NsiI ATGCAT 1 cut(s) 448
NspI RCATGY 1 cut(s) 131
OliI CACNNNNGTG 1 cut(s) 272
PaeI GCATGC 1 cut(s) 131
PdmI GAANNNNTTC 3 cut(s) 268, 345, 600
PfeI GAWTC 2 cut(s) 518, 932
PkrI GCNGC 3 cut(s) 302, 860, 1012
PleI GAGTC 2 cut(s) 286, 654
PpsI GAGTC 2 cut(s) 286, 654
PshBI ATTAAT 1 cut(s) 939
Psp6I CCWGG 1 cut(s) 501
PspFI CCCAGC 1 cut(s) 116
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 216
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 3 cut(s) 158, 598, 901
RsaI GTAC 3 cut(s) 648, 669, 782
RsaNI GTAC 3 cut(s) 647, 668, 781
RseI CAYNNNNRTG 2 cut(s) 272, 451
SaqAI TTAA 3 cut(s) 59, 164, 939
SatI GCNGC 3 cut(s) 301, 859, 1011
Sau3AI GATC 5 cut(s) 158, 251, 598, 901, 1155
ScaI AGTACT 2 cut(s) 648, 669
SchI GAGTC 2 cut(s) 286, 654
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 2 cut(s) 102, 217
SexAI ACCWGGT 1 cut(s) 501
SfaNI GCATC 5 cut(s) 380, 455, 757, 1039, 1071
SfcI CTRYAG 1 cut(s) 1011
SmiMI CAYNNNNRTG 2 cut(s) 272, 451
SphI GCATGC 1 cut(s) 131
Sse9I AATT 4 cut(s) 316, 538, 936, 959
SsiI CCGC 4 cut(s) 301, 710, 1030, 1052
SspI AATATT 1 cut(s) 85
SspMI CTAG 4 cut(s) 141, 261, 396, 579
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 3 cut(s) 355, 995, 1285
TaqI TCGA 1 cut(s) 895
TasI AATT 4 cut(s) 316, 538, 936, 959
TatI WGTACW 3 cut(s) 646, 667, 780
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 2 cut(s) 518, 932
Tru1I TTAA 3 cut(s) 59, 164, 939
Tru9I TTAA 3 cut(s) 59, 164, 939
TscAI CASTG 4 cut(s) 279, 619, 1000, 1288
TseI GCWGC 2 cut(s) 858, 1010
TspDTI ATGAA 3 cut(s) 417, 826, 1278
TspGWI ACGGA 2 cut(s) 300, 1255
TspRI CASTG 4 cut(s) 279, 619, 1000, 1288
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 3 cut(s) 316, 538, 959
XbaI TCTAGA 1 cut(s) 260
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XmnI GAANNNNTTC 3 cut(s) 268, 345, 600
XspI CTAG 4 cut(s) 141, 261, 396, 579
ZrmI AGTACT 2 cut(s) 648, 669
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.