Rroxscaffold_7G00158740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013621
Physical Location & Seq
Forward (+)
2080547 .. 2080999
453 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00158740.1

Sequence Viewer

Length: 453 bp
ATGGAAGAGAGCAGCAGACATTGCTCTGAAGCAGCTGCTTTTTTTCTTGGGGAGAAGGCACTCAAATGGTGCCTCATTGCTTTCTTTGTGTTGAGCTTCTTTTCAGATGCCATATTTTCGATCTCCAAGAACAGAGAACTGGTTATACCTTTTGGTCTCTTTGTTGGGTGCTTAGTTACCGACATTTTGAAAGAGACATTGCAGCAAGTGCTTCCCATTTCAGAGGAGAAGGGATTCGAAAAGCACCTTATCGGAATCGGCTGCCTCTTTGCTGCTGTTAAGTTCATCTCTTATGGTCTCCCAGTACCGGCGCAGGTGTTTCTTCTAAATGTGGCAAATGGCGGGTTCCTGCAAATTTTGTGGCTCTGGAGAGGTTTGTTGAACAAAAGTGACGAAGAAGATGATGTTGGTAATAGTACCAATGCTTCATTGGCCATGGATGGGAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.58

Weight (kDa)

4.98

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 304
Acc36I ACCTGC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 69
AciI CCGC 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 2 cut(s) 306, 418
AfiI CCNNNNNNNGG 2 cut(s) 307, 441
AgsI TTSAA 2 cut(s) 190, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 390, 422
AluBI AGCT 2 cut(s) 35, 96
AluI AGCT 2 cut(s) 35, 96
Alw26I GTCTC 3 cut(s) 161, 188, 302
AoxI GGCC 1 cut(s) 432
ApeKI GCWGC 6 cut(s) 12, 32, 35, 202, 261, 272
ApoI RAATTY 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 237
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 1 cut(s) 69
BarI GAAGNNNNNNTAC 2 cut(s) 409, 441
BbvI GCAGC 6 cut(s) 22, 24, 44, 214, 248, 259
BccI CCATC 1 cut(s) 434
BcoDI GTCTC 3 cut(s) 161, 188, 302
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 6 cut(s) 13, 33, 36, 203, 262, 273
BlsI GCNGC 6 cut(s) 14, 34, 37, 204, 263, 274
BmiI GGNNCC 2 cut(s) 71, 347
BmrI ACTGGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 237
BsaI GGTCTC 2 cut(s) 161, 302
BsaJI CCNNGG 1 cut(s) 435
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 441
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 144, 302
Bse3DI GCAATG 3 cut(s) 19, 75, 197
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 2 cut(s) 307, 441
BseMI GCAATG 3 cut(s) 19, 75, 197
BseNI ACTGG 2 cut(s) 144, 302
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 6 cut(s) 22, 24, 44, 214, 248, 259
BshFI GGCC 1 cut(s) 434
BshNI GGYRCC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 307, 441
BsmAI GTCTC 3 cut(s) 161, 188, 302
BsnI GGCC 1 cut(s) 434
Bso31I GGTCTC 2 cut(s) 161, 302
Bsp119I TTCGAA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 120
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 1 cut(s) 342
BspANI GGCC 1 cut(s) 434
BspLI GGNNCC 2 cut(s) 71, 347
BspMI ACCTGC 1 cut(s) 304
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 161, 302
BsrDI GCAATG 3 cut(s) 19, 75, 197
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 2 cut(s) 144, 302
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 435
BstAPI GCANNNNNTGC 2 cut(s) 21, 208
BstBI TTCGAA 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 172
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 1 cut(s) 123
BstMAI GTCTC 3 cut(s) 161, 188, 302
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 3 cut(s) 21, 208, 431
BstV1I GCAGC 6 cut(s) 22, 24, 44, 214, 248, 259
BsuRI GGCC 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 1 cut(s) 445
BveI ACCTGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 307
Csp6I GTAC 2 cut(s) 305, 417
CspCI CAANNNNNGTGG 2 cut(s) 341, 376
CviAII CATG 1 cut(s) 436
CviJI RGCY 5 cut(s) 35, 96, 261, 364, 434
CviKI_1 RGCY 5 cut(s) 35, 96, 261, 364, 434
CviQI GTAC 2 cut(s) 305, 417
DdeI CTNAG 1 cut(s) 172
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 435
Eco31I GGTCTC 2 cut(s) 161, 302
Eco57I CTGAAG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 1 cut(s) 439
FaiI YATR 4 cut(s) 113, 146, 294, 437
FatI CATG 1 cut(s) 435
FauI CCCGC 1 cut(s) 335
Fnu4HI GCNGC 6 cut(s) 13, 33, 36, 203, 262, 273
Fsp4HI GCNGC 6 cut(s) 13, 33, 36, 203, 262, 273
GlaI GCGC 1 cut(s) 312
GluI GCNGC 6 cut(s) 13, 33, 36, 203, 262, 273
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 1 cut(s) 434
HapII CCGG 1 cut(s) 308
HhaI GCGC 1 cut(s) 313
Hin1II CATG 1 cut(s) 439
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HinfI GANTC 2 cut(s) 234, 255
HpaII CCGG 1 cut(s) 308
Hpy188I TCNGA 4 cut(s) 28, 106, 223, 254
Hpy188III TCNNGA 1 cut(s) 367
HpyAV CCTTC 2 cut(s) 49, 223
HpyCH4V TGCA 2 cut(s) 202, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 208, 431
HpyF3I CTNAG 1 cut(s) 172
Hsp92II CATG 1 cut(s) 439
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 1 cut(s) 120
LpnPI CCDG 6 cut(s) 125, 299, 315, 321, 352, 362
Lsp1109I GCAGC 6 cut(s) 22, 24, 44, 214, 248, 259
LweI GCATC 1 cut(s) 97
MaeIII GTNAC 2 cut(s) 175, 389
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 4 cut(s) 17, 314, 407, 410
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 1 cut(s) 354
MluNI TGGCCA 1 cut(s) 434
MnlI CCTC 4 cut(s) 83, 217, 275, 365
Mox20I TGGCCA 1 cut(s) 434
MroXI GAANNNNTTC 1 cut(s) 233
MscI TGGCCA 1 cut(s) 434
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 21, 208, 431
NcoI CCATGG 1 cut(s) 435
NdeII GATC 1 cut(s) 120
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 2 cut(s) 71, 347
NmuCI GTSAC 1 cut(s) 389
NspV TTCGAA 1 cut(s) 237
PaqCI CACCTGC 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 234, 255
PkrI GCNGC 6 cut(s) 14, 34, 37, 204, 263, 274
PspN4I GGNNCC 2 cut(s) 71, 347
PsrI GAACNNNNNNTAC 2 cut(s) 129, 161
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 306, 418
RsaNI GTAC 2 cut(s) 305, 417
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 6 cut(s) 13, 33, 36, 203, 262, 273
Sau3AI GATC 1 cut(s) 120
SetI ASST 6 cut(s) 37, 98, 151, 249, 318, 376
SfaNI GCATC 1 cut(s) 97
SfuI TTCGAA 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 1 cut(s) 354
SsiI CCGC 1 cut(s) 342
StyI CCWWGG 1 cut(s) 435
TaqI TCGA 2 cut(s) 119, 237
TasI AATT 1 cut(s) 354
TfiI GAWTC 2 cut(s) 234, 255
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 6 cut(s) 12, 32, 35, 202, 261, 272
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 274, 417
XapI RAATTY 1 cut(s) 354
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.