Rroxscaffold_7G00177600

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016263
Physical Location & Seq
Reverse (-)
17151924 .. 17154437
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177600.1

Sequence Viewer

Length: 891 bp
ATGAAGCTGAAAGTAGGCAATTCAGTGGAGGTGTTGAGAAAAGAAAATGATCTGTGTATGTCCTGGTTTTCAGGTAAAGTAATGGCAGTTGATGGCGATGACTTCATTGTGAGGTATAAGTCACTTATAGACCGCAAAGAGGTGATGGCGGTGGAGAGGGTGTACAGAAAGGCCATTAGGCCTCGGCCTCCACATGCAAAGAAAGAGAAGGGATGGATTGTTGGTGATATGGTTGAAGCGTTTGATACGAAGTGTTGGAAGGTTGGGACGGTAGCCAAGGTAGGGAAGAACAATCGAGTTGTCATTAGATTTCTCGGGTCCATTCAGCTTAAGGAATTTGATCAATGTAACCTGAGGATCAGGCAAGCTTGGGATCAGAATAAGTGGTCAGTGATTGAGAAGGTTCAAAAGAAAAATCAGATTAAGATTCGGAGCCATAGATGGGATCATTCAAAAGTTCAACTAGAGGCAAGTTGGGCCGAACCTTTCTCAAAAGAGAAGAGAATTAGCTGCAAGAGAAGAAGATCAAACAGTAATGTGGGAGCATGTGACAGGCCATCAATGAGTAACCATTGGCTGTACATGCAGGTAGATAAAACATCAAGGAACAACTCTAGTACTGATATAGATCCCAAAATGAGAAAGGCAACCAGCTACGGGATGCATAGATCTTTTGAGTCTACTCAGTGCACTGAGGATAGTTACCAGTGTTCTGTGGCTAGTTGTAGTATGAATGAGCTTTCCGGCTCTCAAAATGGGAGTTCTGCAGAACTTGGAGAAGATATTTGTGATAGTTCTGACGCAGAGTCATCATTTCCATCTTCGGCTGGGAAAAACTCTCAGCTCCATTACCAGAGCCTTATGTGCTCGATGTTGACATCCACAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

296

Amino Acids

33.81

Weight (kDa)

9.34

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 6 - 64 4.1e-12 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccI GTMKAC 1 cut(s) 680
AciI CCGC 2 cut(s) 133, 149
AclWI GGATC 4 cut(s) 365, 381, 453, 623
AcsI RAATTY 1 cut(s) 335
AfaI GTAC 3 cut(s) 164, 581, 619
AfiI CCNNNNNNNGG 4 cut(s) 139, 282, 442, 657
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 4 cut(s) 236, 407, 453, 461
AhdI GACNNNNNGTC 1 cut(s) 805
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 7 cut(s) 7, 328, 368, 510, 654, 739, 844
AluI AGCT 7 cut(s) 7, 328, 368, 510, 654, 739, 844
Alw21I GWGCWC 2 cut(s) 692, 869
Alw44I GTGCAC 1 cut(s) 688
AlwI GGATC 4 cut(s) 365, 381, 453, 623
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 5 cut(s) 171, 179, 185, 477, 554
ApaLI GTGCAC 1 cut(s) 688
ApeKI GCWGC 1 cut(s) 510
ApoI RAATTY 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 318, 477
AsuHPI GGTGA 2 cut(s) 154, 236
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 318
AxyI CCTNAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 692
Bbv12I GWGCWC 2 cut(s) 692, 869
BbvI GCAGC 1 cut(s) 497
BccI CCATC 6 cut(s) 86, 139, 207, 435, 565, 826
BciT130I CCWGG 1 cut(s) 64
BclI TGATCA 1 cut(s) 340
BfaI CTAG 3 cut(s) 464, 615, 720
BfmI CTRYAG 1 cut(s) 765
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 577
BglII AGATCT 1 cut(s) 668
BisI GCNGC 1 cut(s) 511
BlsI GCNGC 1 cut(s) 512
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 1 cut(s) 318
BmeRI GACNNNNNGTC 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 318, 477
BmiI GGNNCC 2 cut(s) 319, 434
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 182, 276
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 282, 442, 657
Bse1I ACTGG 1 cut(s) 706
Bse21I CCTNAGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 182, 276
BseGI GGATG 3 cut(s) 218, 666, 878
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 4 cut(s) 139, 282, 442, 657
BseMII CTCAG 4 cut(s) 344, 684, 698, 854
BseNI ACTGG 1 cut(s) 706
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 1 cut(s) 497
BseYI CCCAGC 1 cut(s) 827
BshFI GGCC 5 cut(s) 173, 181, 187, 479, 556
BsiHKAI GWGCWC 2 cut(s) 692, 869
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 744
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 4 cut(s) 139, 282, 442, 657
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 5 cut(s) 173, 181, 187, 479, 556
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 692, 869
Bsp1407I TGTACA 2 cut(s) 162, 579
Bsp143I GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
BspACI CCGC 2 cut(s) 133, 149
BspANI GGCC 5 cut(s) 173, 181, 187, 479, 556
BspCNI CTCAG 4 cut(s) 345, 685, 697, 853
BspLI GGNNCC 2 cut(s) 319, 434
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 4 cut(s) 365, 381, 453, 623
BspTI CTTAAG 1 cut(s) 329
BsrGI TGTACA 2 cut(s) 162, 579
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 182, 276
BssMI GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 271, 533
Bst6I CTCTTC 1 cut(s) 494
BstAFI CTTAAG 1 cut(s) 329
BstAUI TGTACA 2 cut(s) 162, 579
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 4 cut(s) 353, 684, 693, 840
BstF5I GGATG 3 cut(s) 218, 666, 878
BstKTI GATC 8 cut(s) 52, 343, 360, 376, 448, 527, 631, 671
BstMBI GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
BstMWI GCNNNNNNNGC 3 cut(s) 476, 583, 864
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 3 cut(s) 197, 549, 586
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 692
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 2 cut(s) 628, 668
BstYI RGATCY 2 cut(s) 628, 668
Bsu36I CCTNAGG 1 cut(s) 353
BsuRI GGCC 5 cut(s) 173, 181, 187, 479, 556
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 3 cut(s) 218, 666, 878
BtsIMutI CAGTG 5 cut(s) 30, 396, 690, 692, 713
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 318, 477
CseI GACGC 1 cut(s) 809
Csp6I GTAC 3 cut(s) 163, 580, 618
CviAII CATG 3 cut(s) 194, 546, 583
CviQI GTAC 3 cut(s) 163, 580, 618
DdeI CTNAG 4 cut(s) 353, 684, 693, 840
DpnI GATC 8 cut(s) 51, 342, 359, 375, 447, 526, 630, 670
DpnII GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
DriI GACNNNNNGTC 1 cut(s) 805
Eam1104I CTCTTC 1 cut(s) 494
Eam1105I GACNNNNNGTC 1 cut(s) 805
EarI CTCTTC 1 cut(s) 494
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 318
Eco81I CCTNAGG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 276
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 3 cut(s) 197, 549, 586
FaqI GGGAC 1 cut(s) 280
FatI CATG 3 cut(s) 193, 545, 582
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 680
Fnu4HI GCNGC 1 cut(s) 511
FokI GGATG 3 cut(s) 225, 673, 865
Fsp4HI GCNGC 1 cut(s) 511
FspBI CTAG 3 cut(s) 464, 615, 720
GluI GCNGC 1 cut(s) 511
GsaI CCCAGC 1 cut(s) 831
HaeIII GGCC 5 cut(s) 173, 181, 187, 479, 556
HapII CCGG 1 cut(s) 744
HgaI GACGC 1 cut(s) 809
Hin1II CATG 3 cut(s) 197, 549, 586
HincII GTYRAC 1 cut(s) 876
HindII GTYRAC 1 cut(s) 876
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 3 cut(s) 427, 677, 806
HpaII CCGG 1 cut(s) 744
HphI GGTGA 2 cut(s) 154, 236
Hpy166II GTNNAC 4 cut(s) 163, 681, 690, 876
Hpy188I TCNGA 4 cut(s) 378, 420, 432, 799
Hpy8I GTNNAC 4 cut(s) 163, 681, 690, 876
HpyAV CCTTC 3 cut(s) 202, 253, 394
HpyCH4III ACNGT 2 cut(s) 271, 533
HpyCH4V TGCA 6 cut(s) 197, 513, 586, 664, 690, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 583, 864
HpyF3I CTNAG 4 cut(s) 353, 684, 693, 840
Hsp92II CATG 3 cut(s) 197, 549, 586
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
LmnI GCTCC 3 cut(s) 432, 542, 849
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 651
MaeI CTAG 3 cut(s) 464, 615, 720
MaeIII GTNAC 5 cut(s) 120, 347, 548, 566, 701
MalI GATC 8 cut(s) 51, 342, 359, 375, 447, 526, 630, 670
MboI GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
MboII GAAGA 6 cut(s) 298, 511, 531, 534, 791, 813
MflI RGATCY 2 cut(s) 628, 668
MhlI GDGCHC 2 cut(s) 692, 869
MluCI AATT 4 cut(s) 19, 335, 504, 886
MlyI GAGTC 2 cut(s) 686, 815
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 9 cut(s) 22, 105, 133, 150, 192, 198, 348, 460, 688
Mph1103I ATGCAT 1 cut(s) 666
MseI TTAA 2 cut(s) 330, 423
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 1 cut(s) 744
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 3 cut(s) 476, 583, 864
NdeII GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
NlaIII CATG 3 cut(s) 197, 549, 586
NlaIV GGNNCC 2 cut(s) 319, 434
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 120, 548
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 3 cut(s) 197, 549, 586
PceI AGGCCT 1 cut(s) 181
PfeI GAWTC 1 cut(s) 427
PkrI GCNGC 1 cut(s) 512
PleI GAGTC 2 cut(s) 685, 814
PpsI GAGTC 2 cut(s) 685, 814
Psp6I CCWGG 1 cut(s) 62
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 2 cut(s) 319, 434
PspPI GGNCC 2 cut(s) 318, 477
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 2 cut(s) 628, 668
RsaI GTAC 3 cut(s) 164, 581, 619
RsaNI GTAC 3 cut(s) 163, 580, 618
SaqAI TTAA 2 cut(s) 330, 423
SatI GCNGC 1 cut(s) 511
Sau3AI GATC 8 cut(s) 49, 340, 357, 373, 445, 524, 628, 668
Sau96I GGNCC 2 cut(s) 318, 477
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 2 cut(s) 686, 815
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 692, 869
SfaNI GCATC 1 cut(s) 651
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 4 cut(s) 19, 335, 504, 886
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 2 cut(s) 133, 149
SspMI CTAG 3 cut(s) 464, 615, 720
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 2 cut(s) 271, 533
TaqI TCGA 2 cut(s) 295, 869
TasI AATT 4 cut(s) 19, 335, 504, 886
TatI WGTACW 3 cut(s) 162, 579, 617
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 2 cut(s) 330, 423
Tru9I TTAA 2 cut(s) 330, 423
TscAI CASTG 5 cut(s) 30, 396, 692, 697, 713
TseFI GTSAC 2 cut(s) 120, 548
TseI GCWGC 1 cut(s) 510
Tsp45I GTSAC 2 cut(s) 120, 548
TspDTI ATGAA 3 cut(s) 17, 94, 746
TspRI CASTG 5 cut(s) 30, 396, 692, 697, 713
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 335
XceI RCATGY 3 cut(s) 197, 549, 586
XmiI GTMKAC 1 cut(s) 680
XspI CTAG 3 cut(s) 464, 615, 720
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.