Rh6BG325400

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016263
Physical Location & Seq
Forward (+)
55726718 .. 55732517
5800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG325400.1

Sequence Viewer

Length: 1203 bp
ATGGTGTTACAGAGTAGTAAATTGAATCTCTGTCTGCTGCCCAAAATCAAAGTTTTGGGTTTGAGTGGCGGATTGGGAAGAAACCTGTTTTTGTATCACAGCACCTTGATCCTCTCTGTTGACTTTCTTCAGGTCCAAGCTTCCAAAAACATGAAGCTGAAAGTAGGCAATTCAGTGGAGGTGTTGAGAAAAGAAAATGATCTGTGTATGTCCTGGTTTTCAGGTAAAGTAATGGCAGTTGATGGCGATGACTTCATTGTGAGGTATATGTCACTTATAGACCGCAAAGAGGTGATGGCGGTGGAGAGGGTGTACAGAAAGGCCATTAGGCCTCGGCCTCCACATGCAAAGAAAGAGAAGGGATGGATTGTTGGTGATATGGTTGAAGTGTTTGATACGAAGTGTTGGAAGGTTGGGAAGGTAGCCAAGGTAGGGAAGAACAATCGAGTTGTCATTAGATTTCTCGGGTCCATTCAGCTTAAGGAATTTGATGAATGTAACCTGAGGATCAGGCAAGCTTGGGATCAGAATAAGTGGTCAGTGATTGAGAAGGTTCAAAAGAAAAATCAGGTTAAGATTCGGAGCCATAGATGGGATCATTCAAAAGTTCAACTAGAGGCAAGTTGTGCAGAACCTTTAGCAAAAGAGAAGAGAATTAGCTGCAAGAGAAGAAGATCAAACATTAATGTGGGAGCATGTGACAGGCCATCAATGAGTAACCATTGGCTGTACATGCAGGTAGATAAAACATCAAGGAACAACTCTAGTACTGATATAGATCCCAAAATGAGAAAGGCAACCAGCTATGAGATGCATAGATCTTTTGAGTCTACTCAGTGCACTGAGGATAGTTACCAGTGTTCTGTGGCTAGTTGTAGTATGAATGAGCTTTCCGGATCTCAAAATGGGAGTTCTGCAGAACTTGGAGAAGATATTTGTGATAGTTCTGACGCAGAGTCATCATTTCCATCTTCGGCTGGGAAAAAACTCTCAGCTCCATTACCAGAGCCTTATGTGCTCGATGTTGACATCCACAAATTAGAGCTTCACGCATACAAGTCGACTTTGCAGGTGCTCTATGCTTCAGGTCCCTTGAGTTGGGAGCAGGAATCCCTCCTAACGAATCTTCGTCTGTCCCTTCATATAACAAATGAGGAACATTTGCTGCATTTGAGGCACCTCTTATCTTCTCAGGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

400

Amino Acids

45.47

Weight (kDa)

9.16

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 56 - 114 2.2e-11 Agenet domain
ENT PF03735 343 - 389 3.8e-12 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1060
Acc36I ACCTGC 2 cut(s) 727, 1060
AccB1I GGYRCC 1 cut(s) 1176
AccI GTMKAC 2 cut(s) 830, 1061
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 3 cut(s) 69, 283, 299
AclWI GGATC 6 cut(s) 103, 515, 531, 603, 773, 904
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 2 cut(s) 113, 1068
AfaI GTAC 3 cut(s) 314, 731, 769
AfiI CCNNNNNNNGG 4 cut(s) 289, 432, 592, 1098
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 5 cut(s) 25, 386, 557, 603, 611
AhdI GACNNNNNGTC 1 cut(s) 955
AjnI CCWGG 1 cut(s) 212
AluBI AGCT 9 cut(s) 140, 157, 478, 518, 660, 804, 889, 995, 1045
AluI AGCT 9 cut(s) 140, 157, 478, 518, 660, 804, 889, 995, 1045
Alw21I GWGCWC 3 cut(s) 842, 1020, 1077
Alw44I GTGCAC 1 cut(s) 838
AlwI GGATC 6 cut(s) 103, 515, 531, 603, 773, 904
Ama87I CYCGRG 1 cut(s) 464
Aor13HI TCCGGA 1 cut(s) 893
AoxI GGCC 4 cut(s) 321, 329, 335, 704
ApaLI GTGCAC 1 cut(s) 838
ApeKI GCWGC 3 cut(s) 37, 660, 1165
ApoI RAATTY 1 cut(s) 485
AseI ATTAAT 1 cut(s) 684
AspS9I GGNCC 3 cut(s) 133, 468, 1088
AsuHPI GGTGA 2 cut(s) 304, 386
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 3 cut(s) 133, 468, 1088
AxyI CCTNAGG 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 842
BanI GGYRCC 1 cut(s) 1176
Bbv12I GWGCWC 3 cut(s) 842, 1020, 1077
BbvI GCAGC 3 cut(s) 24, 647, 1152
BccI CCATC 6 cut(s) 236, 289, 357, 585, 715, 976
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 1 cut(s) 214
BfaI CTAG 3 cut(s) 614, 765, 870
BfmI CTRYAG 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 2 cut(s) 727, 1060
BglII AGATCT 1 cut(s) 818
BisI GCNGC 3 cut(s) 38, 661, 1166
BlsI GCNGC 3 cut(s) 39, 662, 1167
BmcAI AGTACT 1 cut(s) 769
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 3 cut(s) 133, 468, 1088
BmeRI GACNNNNNGTC 1 cut(s) 955
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 3 cut(s) 133, 468, 1088
BmiI GGNNCC 4 cut(s) 469, 584, 1090, 1178
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 1114
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 332, 426
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 432, 592, 1098
Bse1I ACTGG 1 cut(s) 856
Bse21I CCTNAGG 1 cut(s) 503
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 893
BseBI CCWGG 1 cut(s) 214
BseDI CCNNGG 2 cut(s) 332, 426
BseGI GGATG 2 cut(s) 368, 1029
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 4 cut(s) 289, 432, 592, 1098
BseMII CTCAG 4 cut(s) 494, 834, 848, 1005
BseNI ACTGG 1 cut(s) 856
BseSI GKGCMC 1 cut(s) 842
BseXI GCAGC 3 cut(s) 24, 647, 1152
BseYI CCCAGC 1 cut(s) 977
BsgI GTGCAG 1 cut(s) 648
BshFI GGCC 4 cut(s) 323, 331, 337, 706
BshNI GGYRCC 1 cut(s) 1176
BsiHKAI GWGCWC 3 cut(s) 842, 1020, 1077
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 2 cut(s) 1074, 1120
BslI CCNNNNNNNGG 4 cut(s) 289, 432, 592, 1098
BsmFI GGGAC 2 cut(s) 1074, 1120
BsnI GGCC 4 cut(s) 323, 331, 337, 706
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 3 cut(s) 842, 1020, 1077
Bsp13I TCCGGA 1 cut(s) 893
Bsp1407I TGTACA 2 cut(s) 312, 729
Bsp143I GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
BspACI CCGC 3 cut(s) 69, 283, 299
BspANI GGCC 4 cut(s) 323, 331, 337, 706
BspCNI CTCAG 4 cut(s) 495, 835, 847, 1004
BspEI TCCGGA 1 cut(s) 893
BspLI GGNNCC 4 cut(s) 469, 584, 1090, 1178
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 2 cut(s) 727, 1060
BspPI GGATC 6 cut(s) 103, 515, 531, 603, 773, 904
BspT107I GGYRCC 1 cut(s) 1176
BspTI CTTAAG 1 cut(s) 479
BsrGI TGTACA 2 cut(s) 312, 729
BsrI ACTGG 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 332, 426
BssMI GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 644
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 2 cut(s) 312, 729
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 5 cut(s) 503, 834, 843, 991, 1191
BstF5I GGATG 2 cut(s) 368, 1029
BstKTI GATC 9 cut(s) 111, 202, 510, 526, 598, 677, 781, 821, 899
BstMBI GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
BstMWI GCNNNNNNNGC 4 cut(s) 626, 733, 1015, 1174
BstNI CCWGG 1 cut(s) 214
BstNSI RCATGY 3 cut(s) 347, 699, 736
BstSCI CCNGG 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 842
BstV1I GCAGC 3 cut(s) 24, 647, 1152
BstX2I RGATCY 3 cut(s) 778, 818, 896
BstYI RGATCY 3 cut(s) 778, 818, 896
Bsu36I CCTNAGG 1 cut(s) 503
BsuRI GGCC 4 cut(s) 323, 331, 337, 706
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 368, 1029
BtsIMutI CAGTG 5 cut(s) 180, 546, 840, 842, 863
BveI ACCTGC 2 cut(s) 727, 1060
Cac8I GCNNGC 1 cut(s) 516
Cfr13I GGNCC 3 cut(s) 133, 468, 1088
CseI GACGC 1 cut(s) 959
Csp6I GTAC 3 cut(s) 313, 730, 768
CviAII CATG 4 cut(s) 151, 344, 696, 733
CviQI GTAC 3 cut(s) 313, 730, 768
DdeI CTNAG 5 cut(s) 503, 834, 843, 991, 1191
DpnI GATC 9 cut(s) 110, 201, 509, 525, 597, 676, 780, 820, 898
DpnII GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
DriI GACNNNNNGTC 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 644
Eam1105I GACNNNNNGTC 1 cut(s) 955
EarI CTCTTC 1 cut(s) 644
EciI GGCGGA 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 331
Eco47I GGWCC 3 cut(s) 133, 468, 1088
Eco57I CTGAAG 2 cut(s) 113, 1068
Eco81I CCTNAGG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 464
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 4 cut(s) 154, 347, 699, 736
FaqI GGGAC 2 cut(s) 1074, 1120
FatI CATG 4 cut(s) 150, 343, 695, 732
FblI GTMKAC 2 cut(s) 830, 1061
Fnu4HI GCNGC 3 cut(s) 38, 661, 1166
FokI GGATG 2 cut(s) 375, 1016
Fsp4HI GCNGC 3 cut(s) 38, 661, 1166
FspBI CTAG 3 cut(s) 614, 765, 870
GluI GCNGC 3 cut(s) 38, 661, 1166
GsaI CCCAGC 1 cut(s) 981
HaeIII GGCC 4 cut(s) 323, 331, 337, 706
HapII CCGG 1 cut(s) 894
HgaI GACGC 1 cut(s) 959
Hin1II CATG 4 cut(s) 154, 347, 699, 736
HincII GTYRAC 3 cut(s) 121, 1027, 1062
HindII GTYRAC 3 cut(s) 121, 1027, 1062
HindIII AAGCTT 2 cut(s) 138, 516
HinfI GANTC 6 cut(s) 25, 577, 827, 956, 1109, 1123
HpaII CCGG 1 cut(s) 894
HphI GGTGA 2 cut(s) 304, 386
Hpy166II GTNNAC 6 cut(s) 121, 313, 831, 840, 1027, 1062
Hpy188I TCNGA 3 cut(s) 528, 582, 949
Hpy188III TCNNGA 1 cut(s) 894
Hpy8I GTNNAC 6 cut(s) 121, 313, 831, 840, 1027, 1062
HpyAV CCTTC 5 cut(s) 352, 403, 412, 544, 1148
HpyCH4V TGCA 9 cut(s) 347, 629, 663, 736, 814, 840, 917, 1069, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 626, 733, 1015, 1174
HpyF3I CTNAG 5 cut(s) 503, 834, 843, 991, 1191
Hsp92II CATG 4 cut(s) 154, 347, 699, 736
Kpn2I TCCGGA 1 cut(s) 893
Kzo9I GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
LmnI GCTCC 4 cut(s) 582, 692, 1000, 1102
Lsp1109I GCAGC 3 cut(s) 24, 647, 1152
LweI GCATC 1 cut(s) 801
MaeI CTAG 3 cut(s) 614, 765, 870
MaeIII GTNAC 6 cut(s) 6, 270, 497, 698, 716, 851
MalI GATC 9 cut(s) 110, 201, 509, 525, 597, 676, 780, 820, 898
MboI GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
MflI RGATCY 3 cut(s) 778, 818, 896
MhlI GDGCHC 3 cut(s) 842, 1020, 1077
MluCI AATT 5 cut(s) 20, 169, 485, 654, 1037
MlyI GAGTC 2 cut(s) 836, 965
MmeI TCCRAC 1 cut(s) 386
Mph1103I ATGCAT 1 cut(s) 816
MroI TCCGGA 1 cut(s) 893
MseI TTAA 3 cut(s) 480, 573, 684
MslI CAYNNNNRTG 1 cut(s) 686
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 1 cut(s) 214
MvaI CCWGG 1 cut(s) 214
MwoI GCNNNNNNNGC 4 cut(s) 626, 733, 1015, 1174
NdeII GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
NlaIII CATG 4 cut(s) 154, 347, 699, 736
NlaIV GGNNCC 4 cut(s) 469, 584, 1090, 1178
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 2 cut(s) 270, 698
NsiI ATGCAT 1 cut(s) 816
NspI RCATGY 3 cut(s) 347, 699, 736
PaqCI CACCTGC 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 331
PfeI GAWTC 4 cut(s) 25, 577, 1109, 1123
PkrI GCNGC 3 cut(s) 39, 662, 1167
PleI GAGTC 2 cut(s) 835, 964
PpsI GAGTC 2 cut(s) 835, 964
PpuMI RGGWCCY 1 cut(s) 1088
PshBI ATTAAT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 4 cut(s) 469, 584, 1090, 1178
PspPI GGNCC 3 cut(s) 133, 468, 1088
PspPPI RGGWCCY 1 cut(s) 1088
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 3 cut(s) 778, 818, 896
RsaI GTAC 3 cut(s) 314, 731, 769
RsaNI GTAC 3 cut(s) 313, 730, 768
RseI CAYNNNNRTG 1 cut(s) 686
SalI GTCGAC 1 cut(s) 1060
SaqAI TTAA 3 cut(s) 480, 573, 684
SatI GCNGC 3 cut(s) 38, 661, 1166
Sau3AI GATC 9 cut(s) 108, 199, 507, 523, 595, 674, 778, 818, 896
Sau96I GGNCC 3 cut(s) 133, 468, 1088
ScaI AGTACT 1 cut(s) 769
SchI GAGTC 2 cut(s) 836, 965
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 3 cut(s) 842, 1020, 1077
SfaNI GCATC 1 cut(s) 801
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 3 cut(s) 133, 468, 1088
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 2 cut(s) 479, 1093
SmoI CTYRAG 2 cut(s) 479, 1093
Sse9I AATT 5 cut(s) 20, 169, 485, 654, 1037
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 3 cut(s) 69, 283, 299
SspMI CTAG 3 cut(s) 614, 765, 870
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 426
TaqI TCGA 3 cut(s) 445, 1020, 1061
TasI AATT 5 cut(s) 20, 169, 485, 654, 1037
TatI WGTACW 3 cut(s) 312, 729, 767
TfiI GAWTC 4 cut(s) 25, 577, 1109, 1123
Tru1I TTAA 3 cut(s) 480, 573, 684
Tru9I TTAA 3 cut(s) 480, 573, 684
TscAI CASTG 5 cut(s) 180, 546, 842, 847, 863
TseFI GTSAC 2 cut(s) 270, 698
TseI GCWGC 3 cut(s) 37, 660, 1165
Tsp45I GTSAC 2 cut(s) 270, 698
TspDTI ATGAA 5 cut(s) 167, 244, 507, 896, 1130
TspRI CASTG 5 cut(s) 180, 546, 842, 847, 863
Vha464I CTTAAG 1 cut(s) 479
VneI GTGCAC 1 cut(s) 838
VpaK11BI GGWCC 3 cut(s) 133, 468, 1088
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 1 cut(s) 485
XceI RCATGY 3 cut(s) 347, 699, 736
XmiI GTMKAC 2 cut(s) 830, 1061
XspI CTAG 3 cut(s) 614, 765, 870
ZrmI AGTACT 1 cut(s) 769
Zsp2I ATGCAT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.