Rw6G027420

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016263
Physical Location & Seq
Forward (+)
51259739 .. 51260985
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027420.1

Sequence Viewer

Length: 1053 bp
ATGAAGCTGAAAGTAGGCAATTCAGTGGAGGTGTTGAGAAAAGAAAATGATCTGTGTATGTCCTGGTTTTCAGGTAAAGTTATGGCAGTTGATGGCGATGAATTCATTGTGAGGTATAAGTCACTTATAGACCGCAAAGAGGTGATGGCGGTGGAGAGGGTGTACAGAAAGGCCATTAGGCCTCGGCCTCCACATGCAAAGAAAGAGAAGGGATGGATTGTTGGTGATATGGTTGAAGTGTTTGATACGAAGTGTTGGAAGGTTGGGAAGGTCGCCAAGGTAGGGAAGAACAATCGAGTTGTCATTAGATTTCTCGGGTCCATTCAGCTTAAGGAATTTGATGAATGTAACCTGAGGATCAGGCAAGCTTGGGATCAGAATAAGTGGTCAGTGATTGAGAAGGTTCAAAAGAATAATCAGATTAAGATTCGGAGCCATAGATGGGATCATTCAAAAGTTCAACTAGAGGCAAGTTGTGCAGAACCTTTCTCAAAAGAGAAGATAATTAGCTGCAAGAGAAGAAGATCAAACATTAATGTGGGAGCATGTGACAGGCCATCAATGAGTAACCATTGGCTGTACATGCAGGTAGATAAAACATCAAGGAACAACTCTAGTACTGATGTAGATCCCAAAATGAGAAAGGCAACCAGCTATGGGATGCATAGATCTTTTGAGTCGACTCAGTGCACTGAGGATAGTTACCAGTGTTCTGTGGCTAGTTGTAGTATGAATGAGCTTTCCGGCTCTCAAAATGGGAGTTCTGCAGAACTTGGAGAAGGTATTTGTGATAGTTCTGACGCAGAGTCATCATTTCCATCTTCGGCTGGGAAAAAACTCTCAGCTCCATTACCAGAGCCTTATGTGCTCGATGTTGACATCCACAAATTAGAGCTTCACGCATACAAGTCGACTTTGCACGTGCTCTATGCTTCAGGTCCCTTGAGTTGGGAGCAGGAATCCCTCCTAACGAATCTTCGTCTGTCCCTTAATATAACAAATGAGGAACATTTGCTGCATGTGAGGCACCTCTTATCTTCTCAGGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

350

Amino Acids

39.87

Weight (kDa)

9.03

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 6 - 64 2.4e-12 Agenet domain
ENT PF03735 293 - 338 1.9e-12 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 1026
AccI GTMKAC 2 cut(s) 680, 911
AciI CCGC 2 cut(s) 133, 149
AclWI GGATC 4 cut(s) 365, 381, 453, 623
AcsI RAATTY 2 cut(s) 101, 335
AcuI CTGAAG 1 cut(s) 918
AcvI CACGTG 1 cut(s) 922
AfaI GTAC 3 cut(s) 164, 581, 619
AfiI CCNNNNNNNGG 5 cut(s) 139, 282, 442, 657, 948
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 4 cut(s) 236, 407, 453, 461
AhdI GACNNNNNGTC 1 cut(s) 805
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 8 cut(s) 7, 328, 368, 510, 654, 739, 845, 895
AluI AGCT 8 cut(s) 7, 328, 368, 510, 654, 739, 845, 895
Alw21I GWGCWC 3 cut(s) 692, 870, 927
Alw44I GTGCAC 1 cut(s) 688
AlwI GGATC 4 cut(s) 365, 381, 453, 623
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 4 cut(s) 171, 179, 185, 554
ApaLI GTGCAC 1 cut(s) 688
ApeKI GCWGC 2 cut(s) 510, 1015
ApoI RAATTY 2 cut(s) 101, 335
AseI ATTAAT 1 cut(s) 534
AspS9I GGNCC 2 cut(s) 318, 938
AsuHPI GGTGA 2 cut(s) 154, 236
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 318, 938
AxyI CCTNAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 692
BanI GGYRCC 1 cut(s) 1026
BbrPI CACGTG 1 cut(s) 922
Bbv12I GWGCWC 3 cut(s) 692, 870, 927
BbvI GCAGC 2 cut(s) 497, 1002
BccI CCATC 6 cut(s) 86, 139, 207, 435, 565, 826
BcgI CGANNNNNNTGC 2 cut(s) 891, 925
BciT130I CCWGG 1 cut(s) 64
BfaI CTAG 3 cut(s) 464, 615, 720
BfmI CTRYAG 1 cut(s) 765
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 577
BglII AGATCT 1 cut(s) 668
BisI GCNGC 2 cut(s) 511, 1016
BlsI GCNGC 2 cut(s) 512, 1017
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 2 cut(s) 318, 938
BmeRI GACNNNNNGTC 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 318, 938
BmiI GGNNCC 4 cut(s) 319, 434, 940, 1028
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 964
BsaAI YACGTR 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 182, 276
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 282, 442, 657, 948
Bse1I ACTGG 1 cut(s) 706
Bse21I CCTNAGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 182, 276
BseGI GGATG 3 cut(s) 218, 666, 879
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 5 cut(s) 139, 282, 442, 657, 948
BseMII CTCAG 4 cut(s) 344, 684, 698, 855
BseNI ACTGG 1 cut(s) 706
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 2 cut(s) 497, 1002
BseYI CCCAGC 1 cut(s) 827
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 4 cut(s) 173, 181, 187, 556
BshNI GGYRCC 1 cut(s) 1026
BsiHKAI GWGCWC 3 cut(s) 692, 870, 927
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 744
BslFI GGGAC 2 cut(s) 924, 970
BslI CCNNNNNNNGG 5 cut(s) 139, 282, 442, 657, 948
BsmFI GGGAC 2 cut(s) 924, 970
BsnI GGCC 4 cut(s) 173, 181, 187, 556
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 3 cut(s) 692, 870, 927
Bsp1407I TGTACA 2 cut(s) 162, 579
Bsp143I GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
BspACI CCGC 2 cut(s) 133, 149
BspANI GGCC 4 cut(s) 173, 181, 187, 556
BspCNI CTCAG 4 cut(s) 345, 685, 697, 854
BspLI GGNNCC 4 cut(s) 319, 434, 940, 1028
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 4 cut(s) 365, 381, 453, 623
BspT107I GGYRCC 1 cut(s) 1026
BspTI CTTAAG 1 cut(s) 329
BsrGI TGTACA 2 cut(s) 162, 579
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 182, 276
BssMI GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 64
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 476
BstAUI TGTACA 2 cut(s) 162, 579
BstBAI YACGTR 1 cut(s) 922
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 5 cut(s) 353, 684, 693, 841, 1041
BstF5I GGATG 3 cut(s) 218, 666, 879
BstKTI GATC 7 cut(s) 52, 360, 376, 448, 527, 631, 671
BstMBI GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
BstMWI GCNNNNNNNGC 4 cut(s) 476, 583, 865, 1024
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 4 cut(s) 197, 549, 586, 1022
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 692
BstV1I GCAGC 2 cut(s) 497, 1002
BstX2I RGATCY 2 cut(s) 628, 668
BstYI RGATCY 2 cut(s) 628, 668
Bsu36I CCTNAGG 1 cut(s) 353
BsuRI GGCC 4 cut(s) 173, 181, 187, 556
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 3 cut(s) 218, 666, 879
BtsIMutI CAGTG 5 cut(s) 30, 396, 690, 692, 713
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 318, 938
CseI GACGC 1 cut(s) 809
Csp6I GTAC 3 cut(s) 163, 580, 618
CviAII CATG 4 cut(s) 194, 546, 583, 1019
CviQI GTAC 3 cut(s) 163, 580, 618
DdeI CTNAG 5 cut(s) 353, 684, 693, 841, 1041
DpnI GATC 7 cut(s) 51, 359, 375, 447, 526, 630, 670
DpnII GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
DriI GACNNNNNGTC 1 cut(s) 805
Eam1105I GACNNNNNGTC 1 cut(s) 805
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 318, 938
Eco57I CTGAAG 1 cut(s) 918
Eco72I CACGTG 1 cut(s) 922
Eco81I CCTNAGG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 938
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 276
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 4 cut(s) 197, 549, 586, 1022
FaqI GGGAC 2 cut(s) 924, 970
FatI CATG 4 cut(s) 193, 545, 582, 1018
FblI GTMKAC 2 cut(s) 680, 911
Fnu4HI GCNGC 2 cut(s) 511, 1016
FokI GGATG 3 cut(s) 225, 673, 866
Fsp4HI GCNGC 2 cut(s) 511, 1016
FspBI CTAG 3 cut(s) 464, 615, 720
GluI GCNGC 2 cut(s) 511, 1016
GsaI CCCAGC 1 cut(s) 831
HaeIII GGCC 4 cut(s) 173, 181, 187, 556
HapII CCGG 1 cut(s) 744
HgaI GACGC 1 cut(s) 809
Hin1II CATG 4 cut(s) 197, 549, 586, 1022
HincII GTYRAC 3 cut(s) 681, 877, 912
HindII GTYRAC 3 cut(s) 681, 877, 912
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 6 cut(s) 427, 677, 682, 806, 959, 973
HpaII CCGG 1 cut(s) 744
HphI GGTGA 2 cut(s) 154, 236
Hpy166II GTNNAC 5 cut(s) 163, 681, 690, 877, 912
Hpy188I TCNGA 4 cut(s) 378, 420, 432, 799
Hpy8I GTNNAC 5 cut(s) 163, 681, 690, 877, 912
HpyAV CCTTC 5 cut(s) 202, 253, 262, 394, 773
HpyCH4IV ACGT 1 cut(s) 921
HpyCH4V TGCA 9 cut(s) 197, 479, 513, 586, 664, 690, 767, 919, 1018
HpyF10VI GCNNNNNNNGC 4 cut(s) 476, 583, 865, 1024
HpyF3I CTNAG 5 cut(s) 353, 684, 693, 841, 1041
HpySE526I ACGT 1 cut(s) 921
Hsp92II CATG 4 cut(s) 197, 549, 586, 1022
Kzo9I GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
LmnI GCTCC 4 cut(s) 432, 542, 850, 952
Lsp1109I GCAGC 2 cut(s) 497, 1002
LweI GCATC 1 cut(s) 651
MaeI CTAG 3 cut(s) 464, 615, 720
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 5 cut(s) 120, 347, 548, 566, 701
MalI GATC 7 cut(s) 51, 359, 375, 447, 526, 630, 670
MboI GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
MboII GAAGA 7 cut(s) 298, 511, 531, 534, 813, 968, 1029
MflI RGATCY 2 cut(s) 628, 668
MhlI GDGCHC 3 cut(s) 692, 870, 927
MluCI AATT 5 cut(s) 19, 101, 335, 504, 887
MlyI GAGTC 3 cut(s) 676, 686, 815
MmeI TCCRAC 1 cut(s) 236
Mph1103I ATGCAT 1 cut(s) 666
MseI TTAA 4 cut(s) 330, 423, 534, 990
MslI CAYNNNNRTG 1 cut(s) 536
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 1 cut(s) 744
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 4 cut(s) 476, 583, 865, 1024
NdeII GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
NlaIII CATG 4 cut(s) 197, 549, 586, 1022
NlaIV GGNNCC 4 cut(s) 319, 434, 940, 1028
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 120, 548
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 4 cut(s) 197, 549, 586, 1022
PceI AGGCCT 1 cut(s) 181
PfeI GAWTC 3 cut(s) 427, 959, 973
PkrI GCNGC 2 cut(s) 512, 1017
PleI GAGTC 3 cut(s) 676, 685, 814
PmaCI CACGTG 1 cut(s) 922
PmlI CACGTG 1 cut(s) 922
PpsI GAGTC 3 cut(s) 676, 685, 814
Ppu21I YACGTR 1 cut(s) 922
PpuMI RGGWCCY 1 cut(s) 938
PshBI ATTAAT 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 62
PspCI CACGTG 1 cut(s) 922
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 4 cut(s) 319, 434, 940, 1028
PspPI GGNCC 2 cut(s) 318, 938
PspPPI RGGWCCY 1 cut(s) 938
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 2 cut(s) 628, 668
RsaI GTAC 3 cut(s) 164, 581, 619
RsaNI GTAC 3 cut(s) 163, 580, 618
RseI CAYNNNNRTG 1 cut(s) 536
SalI GTCGAC 2 cut(s) 679, 910
SaqAI TTAA 4 cut(s) 330, 423, 534, 990
SatI GCNGC 2 cut(s) 511, 1016
Sau3AI GATC 7 cut(s) 49, 357, 373, 445, 524, 628, 668
Sau96I GGNCC 2 cut(s) 318, 938
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 3 cut(s) 676, 686, 815
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 3 cut(s) 692, 870, 927
SfaNI GCATC 1 cut(s) 651
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 2 cut(s) 318, 938
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 329, 943
SmoI CTYRAG 2 cut(s) 329, 943
Sse9I AATT 5 cut(s) 19, 101, 335, 504, 887
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 2 cut(s) 133, 149
SspMI CTAG 3 cut(s) 464, 615, 720
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 276
TaiI ACGT 1 cut(s) 924
TaqI TCGA 4 cut(s) 295, 680, 870, 911
TasI AATT 5 cut(s) 19, 101, 335, 504, 887
TatI WGTACW 3 cut(s) 162, 579, 617
TfiI GAWTC 3 cut(s) 427, 959, 973
Tru1I TTAA 4 cut(s) 330, 423, 534, 990
Tru9I TTAA 4 cut(s) 330, 423, 534, 990
TscAI CASTG 5 cut(s) 30, 396, 692, 697, 713
TseFI GTSAC 2 cut(s) 120, 548
TseI GCWGC 2 cut(s) 510, 1015
Tsp45I GTSAC 2 cut(s) 120, 548
TspDTI ATGAA 5 cut(s) 17, 94, 114, 357, 746
TspRI CASTG 5 cut(s) 30, 396, 692, 697, 713
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 318, 938
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 2 cut(s) 101, 335
XceI RCATGY 4 cut(s) 197, 549, 586, 1022
XmiI GTMKAC 2 cut(s) 680, 911
XspI CTAG 3 cut(s) 464, 615, 720
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.