Rorug01G0313100

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012650
Physical Location & Seq
Reverse (-)
43120450 .. 43124412
3963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0313100.1

Sequence Viewer

Length: 1035 bp
ATGAAACGGGAGAATTTGAAGATGAAGCAACCAAGCAAAGACCAGGATACAAGTTCCACCAAGAGAATTCGCATCATATATGATGATCCCTATGCCACTGATTGTTCGAGTGAAGATGATGAGGAATATAATATTAAGAAAAATAGATCAGTGCGTAATAAGCGCATTGTTTCAGAGATTTTGGTTGGGGGTACCCCATGTGAATCATCTGCAAAGACTTCATTCAATTGCAATACCTATGGCACCAAGTTTGGTACTAGTATGGATTTCGATGACAGTAAGGAAACTCAAAGATGTGCTACCAAATATAAGGGAGTTCGGCGTAGAAAATGGGGAACGTATGCGGCGGAAATTCGAGATCCATTTCGAAAGACTAGATTATGGCTTGGCACTTATACTACTGCAGAGGAGGCTGCTATGGCTTATCAGAAAAAGAGGGTCGAGTTTGATAACATAAAGTTATTGCAAAAGACTAAGAGTAATCTTGAAGAGTTGCAATTATCAGATAAGGCCAAGGGAGTGTGTGAGACCAAGCATAATTATCAAGGCAATCATTTATCAGAGAGTCAGGATTTATCAGCTGATGCTGCTATTGAAACTGTTTCATATGAAGACACCAAAAGTGTGTTTTCTCATCCATCACCTTCATCTGTGCTTGATATTTCTAGTACAGCTGCACAAAATATTGGACTCAGAAAGTCAGATGAAGAGTCCACTTTGGAGGTTTCTGGACGAGAAGGCCCTGTGCCACTAGATTCTGAAGAAGAGCAATACAATTTGAAGGCTCCTGAAAAGCCAACATTGTTACCACCCGTACATTTGAATATTGGGTTTGTGGATAATTACATGCTTGAGTGTTTGGATCGGTATTTTGATGGTATGACTGACATAGTTGATCATCCTGCGTGTGATGATGTAAATGGACTCTTCCTTCCAGCTGTAGTGAGGCCAATGTTAGATTCCTTTGAAGCGTCTAAGCAAGTCCCTCAGCCTGCTTTAAGTGATGTGGTTGCAGGTATGACGGGTAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

38.66

Weight (kDa)

5.49

Isoelectric Point (pI)

61.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 101 - 144 1.8e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1004
Acc65I GGTACC 1 cut(s) 191
AccB1I GGYRCC 2 cut(s) 191, 242
AciI CCGC 2 cut(s) 344, 347
AclWI GGATC 3 cut(s) 80, 353, 870
AcsI RAATTY 3 cut(s) 13, 66, 351
AcuI CTGAAG 1 cut(s) 780
AfaI GTAC 4 cut(s) 193, 256, 670, 816
AgsI TTSAA 7 cut(s) 19, 226, 488, 596, 781, 823, 968
AhlI ACTAGT 1 cut(s) 257
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 2 cut(s) 943, 975
AluBI AGCT 3 cut(s) 581, 674, 938
AluI AGCT 3 cut(s) 581, 674, 938
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 3 cut(s) 80, 353, 870
AoxI GGCC 3 cut(s) 510, 739, 947
ApeKI GCWGC 3 cut(s) 413, 587, 674
ApoI RAATTY 3 cut(s) 13, 66, 351
Asp718I GGTACC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 740
AsuHPI GGTGA 1 cut(s) 633
AsuII TTCGAA 1 cut(s) 367
BanI GGYRCC 2 cut(s) 191, 242
BbsI GAAGAC 1 cut(s) 618
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 3 cut(s) 400, 574, 661
BccI CCATC 2 cut(s) 646, 869
BciT130I CCWGG 1 cut(s) 44
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 1 cut(s) 895
BcoDI GTCTC 1 cut(s) 521
BcuI ACTAGT 1 cut(s) 257
BfaI CTAG 4 cut(s) 258, 375, 666, 752
BfmI CTRYAG 2 cut(s) 402, 939
BfuAI ACCTGC 1 cut(s) 1004
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 4 cut(s) 345, 414, 588, 675
BlsI GCNGC 4 cut(s) 346, 415, 589, 676
Bme1390I CCNGG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 740
BmiI GGNNCC 3 cut(s) 193, 244, 786
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 81, 574
BpiI GAAGAC 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 872
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 2 cut(s) 634, 898
BseMII CTCAG 2 cut(s) 706, 1001
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 3 cut(s) 400, 574, 661
BsgI GTGCAG 1 cut(s) 660
BshFI GGCC 3 cut(s) 512, 741, 949
BshNI GGYRCC 2 cut(s) 191, 242
BslFI GGGAC 1 cut(s) 968
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 968
BsnI GGCC 3 cut(s) 512, 741, 949
Bso31I GGTCTC 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 367
Bsp143I GATC 5 cut(s) 85, 146, 358, 862, 895
BspACI CCGC 2 cut(s) 344, 347
BspANI GGCC 3 cut(s) 512, 741, 949
BspCNI CTCAG 2 cut(s) 705, 1000
BspLI GGNNCC 3 cut(s) 193, 244, 786
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 1004
BspPI GGATC 3 cut(s) 80, 353, 870
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 367
BspT107I GGYRCC 2 cut(s) 191, 242
BspTNI GGTCTC 1 cut(s) 521
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 5 cut(s) 85, 146, 358, 862, 895
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 278, 601
Bst6I CTCTTC 4 cut(s) 483, 702, 759, 932
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 993
BstDEI CTNAG 4 cut(s) 474, 692, 975, 987
BstF5I GGATG 2 cut(s) 634, 898
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 5 cut(s) 88, 149, 361, 865, 898
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 5 cut(s) 85, 146, 358, 862, 895
BstMWI GCNNNNNNNGC 4 cut(s) 160, 410, 419, 587
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 2 cut(s) 402, 939
BstV1I GCAGC 3 cut(s) 400, 574, 661
BstV2I GAAGAC 1 cut(s) 618
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 512, 741, 949
BtsCI GGATG 2 cut(s) 634, 898
BtsIMutI CAGTG 2 cut(s) 96, 156
BveI ACCTGC 1 cut(s) 1004
Cac8I GCNNGC 1 cut(s) 993
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 740
CseI GACGC 1 cut(s) 960
Csp6I GTAC 4 cut(s) 192, 255, 669, 815
CviAII CATG 2 cut(s) 198, 847
CviQI GTAC 4 cut(s) 192, 255, 669, 815
DdeI CTNAG 4 cut(s) 474, 692, 975, 987
DpnI GATC 5 cut(s) 87, 148, 360, 864, 897
DpnII GATC 5 cut(s) 85, 146, 358, 862, 895
Eam1104I CTCTTC 4 cut(s) 483, 702, 759, 932
EarI CTCTTC 4 cut(s) 483, 702, 759, 932
EciI GGCGGA 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 513
Eco31I GGTCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 780
EcoO109I RGGNCCY 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 201, 850
FaqI GGGAC 1 cut(s) 968
FatI CATG 2 cut(s) 197, 846
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 895
Fnu4HI GCNGC 4 cut(s) 345, 414, 588, 675
FokI GGATG 2 cut(s) 621, 885
Fsp4HI GCNGC 4 cut(s) 345, 414, 588, 675
FspBI CTAG 4 cut(s) 258, 375, 666, 752
GlaI GCGC 1 cut(s) 164
GluI GCNGC 4 cut(s) 345, 414, 588, 675
HaeIII GGCC 3 cut(s) 512, 741, 949
HgaI GACGC 1 cut(s) 960
HhaI GCGC 1 cut(s) 165
Hin1II CATG 2 cut(s) 201, 850
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 7 cut(s) 203, 565, 690, 710, 755, 924, 959
HphI GGTGA 1 cut(s) 633
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 7 cut(s) 175, 429, 505, 562, 695, 703, 760
Hpy188III TCNNGA 5 cut(s) 356, 485, 569, 729, 788
Hpy8I GTNNAC 1 cut(s) 714
HpyAV CCTTC 4 cut(s) 654, 731, 775, 941
HpyCH4III ACNGT 2 cut(s) 278, 601
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 7 cut(s) 212, 231, 404, 466, 496, 677, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 410, 419, 587
HpyF3I CTNAG 4 cut(s) 474, 692, 975, 987
HpySE526I ACGT 1 cut(s) 338
Hsp92II CATG 2 cut(s) 201, 850
HspAI GCGC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 5 cut(s) 85, 146, 358, 862, 895
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 3 cut(s) 400, 574, 661
LweI GCATC 2 cut(s) 81, 574
MaeI CTAG 4 cut(s) 258, 375, 666, 752
MaeII ACGT 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 804
MalI GATC 5 cut(s) 87, 148, 360, 864, 897
MboI GATC 5 cut(s) 85, 146, 358, 862, 895
MboII GAAGA 8 cut(s) 31, 125, 500, 623, 719, 773, 776, 919
MfeI CAATTG 1 cut(s) 226
MflI RGATCY 1 cut(s) 358
MluCI AATT 8 cut(s) 13, 66, 226, 351, 497, 538, 775, 841
MlyI GAGTC 4 cut(s) 574, 684, 719, 918
MnlI CCTC 7 cut(s) 115, 400, 403, 429, 715, 939, 996
MseI TTAA 2 cut(s) 135, 998
MspA1I CMGCKG 3 cut(s) 581, 674, 938
MspR9I CCNGG 1 cut(s) 44
MunI CAATTG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 160, 410, 419, 587
NdeI CATATG 1 cut(s) 607
NdeII GATC 5 cut(s) 85, 146, 358, 862, 895
NlaIII CATG 2 cut(s) 201, 850
NlaIV GGNNCC 3 cut(s) 193, 244, 786
NspI RCATGY 1 cut(s) 850
NspV TTCGAA 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 759
PfeI GAWTC 3 cut(s) 203, 755, 959
PkrI GCNGC 4 cut(s) 346, 415, 589, 676
PleI GAGTC 4 cut(s) 573, 684, 718, 918
PpsI GAGTC 4 cut(s) 573, 684, 718, 918
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 193, 244, 786
PspPI GGNCC 1 cut(s) 740
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 3 cut(s) 581, 674, 938
RsaI GTAC 4 cut(s) 193, 256, 670, 816
RsaNI GTAC 4 cut(s) 192, 255, 669, 815
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 2 cut(s) 135, 998
SatI GCNGC 4 cut(s) 345, 414, 588, 675
Sau3AI GATC 5 cut(s) 85, 146, 358, 862, 895
Sau96I GGNCC 1 cut(s) 740
SchI GAGTC 4 cut(s) 574, 684, 719, 918
ScrFI CCNGG 1 cut(s) 44
SetI ASST 8 cut(s) 239, 341, 583, 646, 676, 726, 940, 1018
SfaNI GCATC 2 cut(s) 81, 574
SfcI CTRYAG 2 cut(s) 402, 939
SfuI TTCGAA 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
SpeI ACTAGT 1 cut(s) 257
Sse9I AATT 8 cut(s) 13, 66, 226, 351, 497, 538, 775, 841
SsiI CCGC 2 cut(s) 344, 347
SspI AATATT 3 cut(s) 133, 685, 826
SspMI CTAG 4 cut(s) 258, 375, 666, 752
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 278, 601
TaiI ACGT 1 cut(s) 341
TaqI TCGA 5 cut(s) 107, 270, 355, 367, 441
TasI AATT 8 cut(s) 13, 66, 226, 351, 497, 538, 775, 841
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 3 cut(s) 203, 755, 959
Tru1I TTAA 2 cut(s) 135, 998
Tru9I TTAA 2 cut(s) 135, 998
TscAI CASTG 2 cut(s) 103, 156
TseI GCWGC 3 cut(s) 413, 587, 674
TspDTI ATGAA 7 cut(s) 17, 38, 210, 594, 624, 636, 720
TspRI CASTG 2 cut(s) 103, 156
XapI RAATTY 3 cut(s) 13, 66, 351
XceI RCATGY 1 cut(s) 850
XspI CTAG 4 cut(s) 258, 375, 666, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.