Rh1DG315100

IQ-DOMAIN 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012650
Physical Location & Seq
Forward (+)
54177576 .. 54181646
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG315100.1

Sequence Viewer

Length: 1236 bp
ATGGCTGCAGAGGCGGCTGTTGCTGCTGCTCGGGCTGCCGCAGAGGTTGTACGTCTCACTGGGCTGACTCATTTCCCGGGGAAATCTTTGGAGGAGGTAGCAGCCATCAAGATTCAGACGGCATTCCGAGGGCACTTGGCAAGGAGGGCATTACGGGCTTTGAGAGGGTTGGTGAGGTTGAAATCATTGGTAGAGGGTCAATCTGTTAAACGCCAGGCGACTACCACATTAAAATGCATGCAGACCGTTGCTCGTGTACAATCTGAGATTCGTGCAAGGAGGATTAGAATGTCTGAGGAGAATCAGGCACTTCAGAGACAGATTCAACAGAAACAAGAGAAAGAGTTGAAAGAGATAGAGAAACACAGAGTTGCTTCTATTGGAGACAACTGGAACCATAGCCCCCAATCTAAGGAGGAGGTTGAGGCAAAGCTACAGAGCAGACAAGAGGCTGCTAAGAGAAGAGAAAAGGCCTTGGCTTATTCATTCTCTCATCAGAGAATGCGGAAGAACTCTTCGAAATCTCAACATCCTACGTTCATGGATCCCAGCAATCCCCACTGGGGTTGGAGTTGGTTAGAGAGATGGATGGCTGCAAGACCATGGGAGAGCCAAAGCACATCAGATTACAACGACCGGGCCTCCTTGAGGAGTGCAGCAAGCCGTGCATCAATGTCAGTTGCGGAAATCACCAAAGCTTATGCTCTTCGTGATGTCCACCGCGATAACAAGCCTCCTTCCCCAACTGGCCAAAAATCAAGCCGGCCTCCTAGTCGCCAATCCCCTTCAGCTCTTACCAAGACACCATCTTCATCATGGAGAACCCCAAAAAAGAATGGCTCAGGCGGGGACGAGGACTCCAGAAGTACTTACAGTGTGCAGTCGGAGCGATTCAACAGGAGGCATAGCATTGCAGGGTCCTCAGTAAGAGATGATGAGAGCCTTGCAAGCACGCCTTCAGTTCCTTCTTCAAAAAGATACCTGACACCCACAAGCTCAACGAAGACAAGGTCACGACTGGCAAGCCCGAGCTCATCGGGGTTAGAAAAGAACAGGACACCAACACCAGACAAGGGATCATCACCATCAGTCAGTTCTGTAAAGAAGCGGTTGTCTTACCCTGCTTCCCCTGCTGGACAAAGGAGGCACTCGGGTCCTCCTAGGGTTGGCGCTAGCTCCCCTAAGGGTGCAGCTCACACAGAGAAGAAAGTTAGCAATGGTTGGGGAAGCAAATAG

Protein Analysis

411

Amino Acids

45.42

Weight (kDa)

10.96

Isoelectric Point (pI)

74.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 34 - 52 7.7e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 723
AciI CCGC 7 cut(s) 14, 39, 505, 683, 721, 846, 1108
AclWI GGATC 3 cut(s) 539, 552, 1084
AcoI YGGCCR 1 cut(s) 748
AcuI CTGAAG 3 cut(s) 296, 771, 942
AfaI GTAC 3 cut(s) 51, 258, 868
AfiI CCNNNNNNNGG 5 cut(s) 412, 563, 648, 1073, 1166
AgsI TTSAA 5 cut(s) 181, 326, 349, 895, 972
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 7 cut(s) 433, 698, 791, 996, 1032, 1176, 1193
AluI AGCT 7 cut(s) 433, 698, 791, 996, 1032, 1176, 1193
Alw21I GWGCWC 1 cut(s) 1034
Alw26I GTCTC 3 cut(s) 59, 310, 378
AlwI GGATC 3 cut(s) 539, 552, 1084
Ama87I CYCGRG 4 cut(s) 30, 76, 1027, 1150
AoxI GGCC 4 cut(s) 471, 639, 748, 764
ApeKI GCWGC 9 cut(s) 5, 23, 26, 35, 101, 452, 593, 656, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 995, 1027
AspA2I CCTAGG 1 cut(s) 1160
AspLEI GCGC 1 cut(s) 1172
AspS9I GGNCC 3 cut(s) 639, 918, 1154
AsuC2I CCSGG 3 cut(s) 77, 78, 638
AsuHPI GGTGA 3 cut(s) 184, 682, 1074
AsuII TTCGAA 1 cut(s) 518
AsuNHI GCTAGC 1 cut(s) 1172
AvaI CYCGRG 4 cut(s) 30, 76, 1027, 1150
AvaII GGWCC 2 cut(s) 918, 1154
AvrII CCTAGG 1 cut(s) 1160
AxyI CCTNAGG 1 cut(s) 1182
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 750
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 1034
BauI CACGAG 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 1010
Bbv12I GWGCWC 1 cut(s) 1034
BbvI GCAGC 8 cut(s) 10, 13, 22, 113, 439, 580, 668, 1202
BccI CCATC 5 cut(s) 113, 579, 583, 814, 1093
BceAI ACGGC 2 cut(s) 135, 648
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 3 cut(s) 77, 78, 638
BcoDI GTCTC 3 cut(s) 59, 310, 378
BfaI CTAG 3 cut(s) 771, 1161, 1173
BfmI CTRYAG 2 cut(s) 6, 434
BfoI RGCGCY 1 cut(s) 1173
BlnI CCTAGG 1 cut(s) 1160
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 4 cut(s) 77, 78, 215, 638
Bme18I GGWCC 2 cut(s) 918, 1154
BmeT110I CYCGRG 4 cut(s) 30, 76, 1027, 1150
BmgT120I GGNCC 3 cut(s) 639, 918, 1154
BmiI GGNNCC 4 cut(s) 395, 546, 919, 1155
BmrFI CCNGG 4 cut(s) 77, 78, 215, 638
BmrI ACTGGG 2 cut(s) 69, 571
BmsI GCATC 1 cut(s) 677
BmtI GCTAGC 1 cut(s) 1176
BmuI ACTGGG 2 cut(s) 69, 571
BpiI GAAGAC 1 cut(s) 1010
BpmI CTGGAG 1 cut(s) 844
Bpu10I CCTNAGC 1 cut(s) 841
Bpu14I TTCGAA 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 667
BpuMI CCSGG 3 cut(s) 77, 78, 638
BsaJI CCNNGG 6 cut(s) 76, 77, 127, 474, 602, 1160
BsaXI ACNNNNNCTCC 4 cut(s) 626, 656, 842, 872
Bsc4I CCNNNNNNNGG 5 cut(s) 412, 563, 648, 1073, 1166
Bse118I RCCGGY 1 cut(s) 762
Bse1I ACTGG 5 cut(s) 64, 395, 566, 751, 1023
Bse21I CCTNAGG 1 cut(s) 1182
Bse3DI GCAATG 2 cut(s) 909, 1222
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 6 cut(s) 76, 77, 127, 474, 602, 1160
BseGI GGATG 2 cut(s) 529, 594
BseLI CCNNNNNNNGG 5 cut(s) 412, 563, 648, 1073, 1166
BseMI GCAATG 2 cut(s) 909, 1222
BseMII CTCAG 4 cut(s) 255, 285, 855, 936
BseNI ACTGG 5 cut(s) 64, 395, 566, 751, 1023
BseRI GAGGAG 4 cut(s) 107, 311, 431, 664
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 8 cut(s) 10, 13, 22, 113, 439, 580, 668, 1202
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 3 cut(s) 675, 899, 1209
Bsh1236I CGCG 1 cut(s) 723
Bsh1285I CGRYCG 1 cut(s) 637
BshFI GGCC 4 cut(s) 473, 641, 750, 766
BsiEI CGRYCG 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 1034
BsiHKCI CYCGRG 4 cut(s) 30, 76, 1027, 1150
BsiSI CCGG 3 cut(s) 77, 637, 763
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 5 cut(s) 412, 563, 648, 1073, 1166
BsmAI GTCTC 3 cut(s) 59, 310, 378
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 863
BsmI GAATGC 2 cut(s) 122, 507
BsnI GGCC 4 cut(s) 473, 641, 750, 766
BsoBI CYCGRG 4 cut(s) 30, 76, 1027, 1150
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 135, 1034
Bsp1407I TGTACA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 544, 1076
Bsp19I CCATGG 1 cut(s) 602
BspACI CCGC 7 cut(s) 14, 39, 505, 683, 721, 846, 1108
BspANI GGCC 4 cut(s) 473, 641, 750, 766
BspCNI CTCAG 4 cut(s) 256, 286, 854, 935
BspFNI CGCG 1 cut(s) 723
BspLI GGNNCC 4 cut(s) 395, 546, 919, 1155
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 1176
BspPI GGATC 3 cut(s) 539, 552, 1084
BspQI GCTCTTC 1 cut(s) 711
BspT104I TTCGAA 1 cut(s) 518
BsrDI GCAATG 2 cut(s) 909, 1222
BsrFI RCCGGY 1 cut(s) 762
BsrGI TGTACA 1 cut(s) 256
BsrI ACTGG 5 cut(s) 64, 395, 566, 751, 1023
BssAI RCCGGY 1 cut(s) 762
BssECI CCNNGG 6 cut(s) 76, 77, 127, 474, 602, 1160
BssMI GATC 2 cut(s) 544, 1076
BssSI CACGAG 1 cut(s) 252
BssT1I CCWWGG 3 cut(s) 474, 602, 1160
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 247, 875
Bst6I CTCTTC 3 cut(s) 457, 520, 711
BstAPI GCANNNNNTGC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 7 cut(s) 239, 661, 764, 949, 953, 1024, 1174
BstDEI CTNAG 7 cut(s) 264, 294, 411, 456, 841, 922, 1182
BstDSI CCRYGG 1 cut(s) 602
BstENI CCTNNNNNAGG 1 cut(s) 646
BstF5I GGATG 2 cut(s) 529, 594
BstFNI CGCG 1 cut(s) 723
BstH2I RGCGCY 1 cut(s) 1173
BstHHI GCGC 1 cut(s) 1172
BstKTI GATC 2 cut(s) 547, 1079
BstMAI GTCTC 3 cut(s) 59, 310, 378
BstMBI GATC 2 cut(s) 544, 1076
BstMCI CGRYCG 1 cut(s) 637
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 4 cut(s) 75, 76, 213, 636
BstSFI CTRYAG 2 cut(s) 6, 434
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 8 cut(s) 10, 13, 22, 113, 439, 580, 668, 1202
BstV2I GAAGAC 1 cut(s) 1010
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 1182
BsuRI GGCC 4 cut(s) 473, 641, 750, 766
BtgI CCRYGG 1 cut(s) 602
BtsCI GGATG 2 cut(s) 529, 594
BtsIMutI CAGTG 3 cut(s) 57, 559, 880
Cac8I GCNNGC 7 cut(s) 239, 661, 764, 949, 953, 1024, 1174
CfoI GCGC 1 cut(s) 1172
Cfr10I RCCGGY 1 cut(s) 762
Cfr13I GGNCC 3 cut(s) 639, 918, 1154
Cfr9I CCCGGG 1 cut(s) 76
Csp6I GTAC 3 cut(s) 50, 257, 867
CspCI CAANNNNNGTGG 2 cut(s) 548, 583
CviAII CATG 4 cut(s) 238, 541, 603, 816
CviQI GTAC 3 cut(s) 50, 257, 867
DdeI CTNAG 7 cut(s) 264, 294, 411, 456, 841, 922, 1182
DpnI GATC 2 cut(s) 546, 1078
DpnII GATC 2 cut(s) 544, 1076
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 3 cut(s) 457, 520, 711
EarI CTCTTC 3 cut(s) 457, 520, 711
Ecl136II GAGCTC 1 cut(s) 1032
Eco130I CCWWGG 3 cut(s) 474, 602, 1160
Eco147I AGGCCT 1 cut(s) 473
Eco24I GRGCYC 1 cut(s) 1034
Eco47I GGWCC 2 cut(s) 918, 1154
Eco53kI GAGCTC 1 cut(s) 1032
Eco57I CTGAAG 3 cut(s) 296, 771, 942
Eco81I CCTNAGG 1 cut(s) 1182
Eco88I CYCGRG 4 cut(s) 30, 76, 1027, 1150
EcoICRI GAGCTC 1 cut(s) 1032
EcoNI CCTNNNNNAGG 1 cut(s) 646
EcoO109I RGGNCCY 2 cut(s) 918, 1154
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 3 cut(s) 474, 602, 1160
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 1034
ErhI CCWWGG 3 cut(s) 474, 602, 1160
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 4 cut(s) 241, 544, 606, 819
FaiI YATR 7 cut(s) 239, 399, 542, 604, 702, 817, 906
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 1 cut(s) 863
FatI CATG 4 cut(s) 237, 540, 602, 815
FauI CCCGC 1 cut(s) 839
FokI GGATG 2 cut(s) 516, 601
FriOI GRGCYC 1 cut(s) 1034
FspBI CTAG 3 cut(s) 771, 1161, 1173
GlaI GCGC 1 cut(s) 1171
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 844
HaeII RGCGCY 1 cut(s) 1173
HaeIII GGCC 4 cut(s) 473, 641, 750, 766
HapII CCGG 3 cut(s) 77, 637, 763
HhaI GCGC 1 cut(s) 1172
Hin1II CATG 4 cut(s) 241, 544, 606, 819
Hin6I GCGC 1 cut(s) 1170
HinP1I GCGC 1 cut(s) 1170
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 7 cut(s) 67, 112, 268, 301, 322, 857, 891
HpaII CCGG 3 cut(s) 77, 637, 763
HphI GGTGA 3 cut(s) 184, 682, 1074
Hpy166II GTNNAC 2 cut(s) 257, 718
Hpy188I TCNGA 8 cut(s) 117, 128, 265, 295, 315, 498, 625, 886
Hpy188III TCNNGA 4 cut(s) 109, 710, 861, 1014
Hpy8I GTNNAC 2 cut(s) 257, 718
HpyAV CCTTC 4 cut(s) 747, 795, 966, 975
HpyCH4III ACNGT 2 cut(s) 247, 875
HpyCH4IV ACGT 2 cut(s) 52, 536
HpyF3I CTNAG 7 cut(s) 264, 294, 411, 456, 841, 922, 1182
HpySE526I ACGT 2 cut(s) 52, 536
Hsp92II CATG 4 cut(s) 241, 544, 606, 819
HspAI GCGC 1 cut(s) 1170
KroI GCCGGC 1 cut(s) 762
KroNI GCCGGC 1 cut(s) 764
Kzo9I GATC 2 cut(s) 544, 1076
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 2 cut(s) 886, 1181
Lsp1109I GCAGC 8 cut(s) 10, 13, 22, 113, 439, 580, 668, 1202
LweI GCATC 1 cut(s) 677
MaeI CTAG 3 cut(s) 771, 1161, 1173
MaeII ACGT 2 cut(s) 52, 536
MaeIII GTNAC 1 cut(s) 1011
MalI GATC 2 cut(s) 546, 1078
MboI GATC 2 cut(s) 544, 1076
MboII GAAGA 8 cut(s) 474, 507, 520, 698, 801, 960, 1015, 1216
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 2 cut(s) 135, 1034
MlsI TGGCCA 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 2 cut(s) 61, 851
MmeI TCCRAC 2 cut(s) 548, 864
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 1 cut(s) 239
MroNI GCCGGC 1 cut(s) 762
MscI TGGCCA 1 cut(s) 750
MseI TTAA 2 cut(s) 207, 230
MslI CAYNNNNRTG 1 cut(s) 232
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 3 cut(s) 77, 637, 763
MspR9I CCNGG 4 cut(s) 77, 78, 215, 638
Mva1269I GAATGC 2 cut(s) 122, 507
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 723
NaeI GCCGGC 1 cut(s) 764
NciI CCSGG 3 cut(s) 77, 78, 638
NcoI CCATGG 1 cut(s) 602
NdeII GATC 2 cut(s) 544, 1076
NgoMIV GCCGGC 1 cut(s) 762
NheI GCTAGC 1 cut(s) 1172
NlaIII CATG 4 cut(s) 241, 544, 606, 819
NlaIV GGNNCC 4 cut(s) 395, 546, 919, 1155
NmuCI GTSAC 1 cut(s) 1011
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 1 cut(s) 518
PaeI GCATGC 1 cut(s) 241
PceI AGGCCT 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 711
PctI GAATGC 2 cut(s) 122, 507
PdiI GCCGGC 1 cut(s) 764
PfeI GAWTC 5 cut(s) 112, 268, 301, 322, 891
PflFI GACNNNGTC 1 cut(s) 1009
PleI GAGTC 2 cut(s) 61, 851
PpsI GAGTC 2 cut(s) 61, 851
PpuMI RGGWCCY 2 cut(s) 918, 1154
Psp124BI GAGCTC 1 cut(s) 1034
Psp5II RGGWCCY 2 cut(s) 918, 1154
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 4 cut(s) 395, 546, 919, 1155
PspPI GGNCC 3 cut(s) 639, 918, 1154
PspPPI RGGWCCY 2 cut(s) 918, 1154
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 544
PsyI GACNNNGTC 1 cut(s) 1009
RsaI GTAC 3 cut(s) 51, 258, 868
RsaNI GTAC 3 cut(s) 50, 257, 867
RseI CAYNNNNRTG 1 cut(s) 232
SacI GAGCTC 1 cut(s) 1034
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 2 cut(s) 207, 230
Sau3AI GATC 2 cut(s) 544, 1076
Sau96I GGNCC 3 cut(s) 639, 918, 1154
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 2 cut(s) 61, 851
ScrFI CCNGG 4 cut(s) 77, 78, 215, 638
SduI GDGCHC 2 cut(s) 135, 1034
SfaNI GCATC 1 cut(s) 677
SfcI CTRYAG 2 cut(s) 6, 434
SfuI TTCGAA 1 cut(s) 518
SinI GGWCC 2 cut(s) 918, 1154
SmaI CCCGGG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SphI GCATGC 1 cut(s) 241
SseBI AGGCCT 1 cut(s) 473
SsiI CCGC 7 cut(s) 14, 39, 505, 683, 721, 846, 1108
SspMI CTAG 3 cut(s) 771, 1161, 1173
SstI GAGCTC 1 cut(s) 1034
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 4 cut(s) 75, 76, 213, 636
StyI CCWWGG 3 cut(s) 474, 602, 1160
TaaI ACNGT 2 cut(s) 247, 875
TaiI ACGT 2 cut(s) 55, 539
TaqI TCGA 1 cut(s) 518
TatI WGTACW 2 cut(s) 256, 866
TauI GCSGC 2 cut(s) 17, 41
TfiI GAWTC 5 cut(s) 112, 268, 301, 322, 891
Tru1I TTAA 2 cut(s) 207, 230
Tru9I TTAA 2 cut(s) 207, 230
TscAI CASTG 3 cut(s) 64, 566, 880
TseFI GTSAC 1 cut(s) 1011
TseI GCWGC 9 cut(s) 5, 23, 26, 35, 101, 452, 593, 656, 1190
Tsp45I GTSAC 1 cut(s) 1011
TspDTI ATGAA 3 cut(s) 474, 529, 801
TspMI CCCGGG 1 cut(s) 76
TspRI CASTG 3 cut(s) 64, 566, 880
Tth111I GACNNNGTC 1 cut(s) 1009
VpaK11BI GGWCC 2 cut(s) 918, 1154
XagI CCTNNNNNAGG 1 cut(s) 646
XceI RCATGY 1 cut(s) 241
XcmI CCANNNNNNNNNTGG 1 cut(s) 813
XmaI CCCGGG 1 cut(s) 76
XmaJI CCTAGG 1 cut(s) 1160
XspI CTAG 3 cut(s) 771, 1161, 1173
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.