Rorug02G0094300

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017772
Physical Location & Seq
Forward (+)
7731643 .. 7740309
8667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0094300.1

Sequence Viewer

Length: 3309 bp
ATGGCCGAGGAGTTCTCCAAAGCCGTCGACGACGGTCTCCGGCTCTCGAAACGACTATACTTAGGGAAGGATCGAGCCGTGGCGCCGCCGAGGCCGCCGCCGGTCATGGACAAGTCGGCGTCGTCGGCGGCGGCGGGGTGCGCCTACATCCCGACGTCTCCGATGGTGTACGCCGTGATATCGGACCCGAGGATCGTCGACAACCCGGACATCCCGAGCTACCAGCCGCACGTGCACGGCAGGTGCGACCCGCCGGCGCTCATTCCGCTTCCGATGAACAGGGTTGAGCTGGAGGTCGATTGCTTCATGGACACCGCCTTTATTCGGGTCACCGGGTCGTGGAGGGTCCATTGCGTCAAGAGCTCCAGGAGCTGTGGCTGCCGGATTGCCATCCCTATGGGCGAACAGGGTTCAATTCTAGGTGTTGAGGTCGACATTTCTGGTAAATCATTCTATACTCAGTTAAGTCAATTGAAAGATGACACGGATATGAGCAAGGTTCCCCAAGGAGAAGGAGGCTTTCTCAATGTTAAACCTCATATATTCACATTGACAACTCCGCCGGTTGATGGGGGAGTCAATCTCACAATTAAAATGCGTTGGTCTCAGAAGTTATTGTATCGAAATGGAGAGTTCTCTTTAGATATACCGTTCACATTTCCGGAGTTTGTCATTCCTCCCGGAAAGAAGTACCTGAAAAAGGAAAAGATTGAATTGAATGTGAACTCTGGTCTGGGAACTGAAATTTTATTCAAGGGAACTAGTCATCTTTTGAAGGAAGGACAGAGCCAAGAAGGAAAATTGGGGTTTAAATACGAGGGAAATGTTGTCAACTGGTCAAGCACCGACTTCCACTTCTCATATGCGGTCTCTTCAAGCCAAATACATGGCACTGTTATCACGCAGTCACCTTCCAAGGATGATGTTGATCAAAGAGAGATTTTCTCTGCCTATCTTCTTCCTGGAAACCAGCAAAGTGGAAAGGGTTTCAGAAGGAATGTAGTGGTTGTTGTTGATATAAGTGGAAGTATGCAGGGAAAGCCACTTGAAGATACTCAGAATGCACTATCTGAAGCCCTCTCTAAACTTGATCCAGAAGATTCATTCTGCATTATAGCTTTTAATGGACAGACTTATGTTTCATCTACATCATTGAAATCGGCTACCAAGGAGGCAGTCGAATCGGCCATTGAGTGGATTGGCATCAACTTTATTGCTGGTGGTGATACAAATATTATGTGTCCCCTTAACATGGCAATAGAGATGCTATCCAACTCTAGGGGTTCACTTCCTATCATATTTCTGGTTACTGATGGGGCAGTTGAAGATGAGAGACAAATATGCGATGAAATAAAAAAGCGTCTTGCAAGTGAGGATGCGATATCCCCTCGTATATACACTTTTGGAATAGGTTCGTTCTGCAACCATTATTTCCTGCGGATGCTATCAACAATTGGAAGGGGCCAATATGATGCTGCCTATGATATAGATTTGGTTCAGCCTCGAATACAGAACTTATTTGCGACAGCTTCATCTGTGATTCTTACAAATATAACCCTTGAGACATTGGACGATCTTGATGATGTTGAGGTGCTCCCTTGCCATATTCCAGACCTCTCATTTGAGAGTCCATTAAGTGTATCTGGCAGATTCCGAGGCAAGTTACCTGAGACCTTCAAAGTTAAAGGATTCTCAGCTGATATGTCGAATATTGTGATCAATTTGAAAGTACAAGATGCAAAGGACATACCTCTTCACAGGGTATGTGCAAAAGGAGAGATTGAGTTACTGACTGCTCAGGCATGGCACTCAGAGAACAAGCAGCTTGAGGATAAGGTAGCTAAGTTGAGTGTACATACCGGTGCTTTGAGCGAGTATACACGCATGGTCATATTTGAAAAAGAAGTAGCTTTGCAACAGATGGCCTCAAAGAAAAGCCAGAACAAGAAGGAGGATACTGAAACCTTGAAAATGATATTGCCACATAGTTTGTGCGTCGGCTTTGGAAACGTGGCCGCAACTTCGGACAACATTCCTCCAGGAGCCGAAGAACCAAAGCTTCCGGAAGCAGTTGAAATTTTCGCCAAAGCCGCTTCAGGCTGTTGTGGTCGCCAAACTCTCATCACAGTTCACCGGAGAACAATTTCACGTCCGATAAGGGTTAATTCAGTTGGCATTTTCACTGAACAAATATGGGTGGTTGCTGTTGTTTTCATGGTACCTGTCTATGCTACTGACTCGATGATATCCTTGTTGAATCCGAAGTTGTCTCTAGCATGTGACATTCTAAGAAGCTGTTATGAAGCGTTTGCCTTGTATTCTTTTGGAAGCTACTTGGTTGCTTGTCTGGGAGGTGAGAGAAGAGTAGTAGAATTACTTGAAAATCAATCTGGAAAGCTCCTAAACAAAGCATTGGTAGAAGGGGAAGATGAAAATTGCAGGAAACCACACTGGTCTCTTCGCAACTTCTTCAGGCGACCAACTATTATTGGGAAAAATTTGCTCACTATAGTGAAATTCGGTCTTGTACAATATATGATTCTGAAGACAGTTTGTGCATTCCTAGCACTCGTGTTGGAAATCTTTGGTGTCTATGGTGATGGGGAATTCAAGTTTCACTACGGGTATCCATACATCACGATAGTACTGAATTTCAGCCAGATGTGGGCATTGTATTGCCTTGTGCAATTCTATAATGTGACTCATGAAAGACTTCAACCAATAAGGCCGCTTGCAAAGTTCATCAGCTTTAAGGCTATTGTGTTTGCCACTTGGTGGCAAGGTGTAGGTATTGCGCTGTTGTGTTATTTTGGTGTTCTTCCCAACCAGGAAAAACTACAAACAGGGTTGCAGGATTTCTTGATTTGTATAGAAATGGCAATTGCAGCTGTTGCACATGTGTTTGTCTTTTCGGCAGAACCTTACCATTACATTCCATCTTCTTCTGGGTATGAGAAGATCACCACCACTGAAACAACCAGTGAGGAAGCAGTGAAGTTGGAGGAAGGTGACAGTAGTACACAAGCTGTGGTTGAAACAAAAGAAACTCAGGTCGAGGCTCCCGGAACAAGTGTGACTGAGAGTGTTCAGGACATAGTGTTTGAAGGTGGTCATCATGTTGTCAAGGACGTTGTGTTGACCATAAATCAAGCAATAGGGCCTGTGGAGAAGGGTGTGACGAAGGGTGTGACAAAGATACAAGAAACCTTCCACCAAAGATCAGATGAGCATGACGATAAGTCGAAGGTTTCAGTTGAGGAGAGTATTGAAGTGACTCTTTCCGATGAAGAAACACATTTGGACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

1102

Amino Acids

122.01

Weight (kDa)

5.54

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA PF00092 333 - 487 4.7e-13 von Willebrand factor type A domain
VWA_3 PF13768 333 - 491 2.7e-10 von Willebrand factor type A domain
VWA_2 PF13519 334 - 439 7e-11 von Willebrand factor type A domain
Solute_trans_a PF03619 729 - 975 5.1e-81 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 231
AatII GACGTC 1 cut(s) 158
Acc36I ACCTGC 1 cut(s) 231
Acc65I GGTACC 1 cut(s) 2218
AccB1I GGYRCC 2 cut(s) 82, 2218
AccB7I CCANNNNNTGG 2 cut(s) 1194, 2775
AccI GTMKAC 4 cut(s) 27, 198, 432, 1877
AccIII TCCGGA 2 cut(s) 661, 2062
AclWI GGATC 3 cut(s) 78, 200, 1085
AcoI YGGCCR 3 cut(s) 3, 1185, 2013
AcsI RAATTY 6 cut(s) 744, 2076, 2497, 2516, 2606, 2650
AcuI CTGAAG 4 cut(s) 1092, 2079, 2455, 2564
AcvI CACGTG 1 cut(s) 232
AcyI GRCGYC 3 cut(s) 83, 119, 155
AdeI CACNNNGTG 1 cut(s) 2775
AfaI GTAC 8 cut(s) 170, 692, 1731, 1854, 2220, 2529, 2646, 3019
AfiI CCNNNNNNNGG 9 cut(s) 324, 339, 569, 700, 1194, 1252, 1278, 2665, 2775
AflIII ACRYGT 1 cut(s) 2896
AgeI ACCGGT 1 cut(s) 1859
AhdI GACNNNNNGTC 1 cut(s) 3238
AhlI ACTAGT 1 cut(s) 761
AjiI CACGTC 1 cut(s) 2150
AjnI CCWGG 4 cut(s) 365, 961, 2038, 2826
AleI CACNNNNGTG 1 cut(s) 2510
Alw21I GWGCWC 3 cut(s) 237, 365, 1596
Alw26I GTCTC 9 cut(s) 41, 162, 609, 874, 1327, 1556, 1664, 2274, 2460
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 3 cut(s) 78, 200, 1085
AlwNI CAGNNNCTG 1 cut(s) 372
Ama87I CYCGRG 2 cut(s) 187, 214
Aor13HI TCCGGA 2 cut(s) 661, 2062
AoxI GGCC 8 cut(s) 3, 92, 1185, 1462, 1923, 2013, 2726, 3158
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 4 cut(s) 378, 1475, 1822, 2885
ApoI RAATTY 6 cut(s) 744, 2076, 2497, 2516, 2606, 2650
ArsI GACNNNNNNTTYG 4 cut(s) 1604, 1636, 3083, 3115
AsiGI ACCGGT 1 cut(s) 1859
Asp700I GAANNNNTTC 3 cut(s) 1411, 2143, 2712
Asp718I GGTACC 1 cut(s) 2218
AspLEI GCGC 4 cut(s) 85, 143, 259, 2797
AspS9I GGNCC 5 cut(s) 184, 346, 1462, 3158, 3301
AsuC2I CCSGG 4 cut(s) 206, 334, 681, 3063
AsuHPI GGTGA 8 cut(s) 322, 900, 1235, 2123, 2366, 2609, 2953, 3020
AvaI CYCGRG 2 cut(s) 187, 214
AvaII GGWCC 3 cut(s) 184, 346, 3301
BaeGI GKGCMC 1 cut(s) 237
BanI GGYRCC 2 cut(s) 82, 2218
BanII GRGCYC 1 cut(s) 365
BauI CACGAG 1 cut(s) 2570
BbrPI CACGTG 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 2552
Bbv12I GWGCWC 3 cut(s) 237, 365, 1596
BbvI GCAGC 4 cut(s) 365, 1462, 1834, 2897
BccI CCATC 7 cut(s) 157, 398, 563, 1307, 1915, 2594, 2944
BceAI ACGGC 4 cut(s) 8, 62, 158, 253
BcgI CGANNNNNNTGC 2 cut(s) 1164, 1198
BciT130I CCWGG 4 cut(s) 367, 963, 2040, 2828
BciVI GTATCC 2 cut(s) 1948, 2637
BclI TGATCA 2 cut(s) 928, 1716
BcnI CCSGG 4 cut(s) 206, 334, 681, 3063
BcoDI GTCTC 9 cut(s) 41, 162, 609, 874, 1327, 1556, 1664, 2274, 2460
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 5 cut(s) 419, 762, 1278, 2273, 2564
BfmI CTRYAG 1 cut(s) 2508
BfoI RGCGCY 2 cut(s) 86, 260
BfuAI ACCTGC 1 cut(s) 231
BfuI GTATCC 2 cut(s) 1948, 2637
BglI GCCNNNNNGGC 1 cut(s) 91
BmcAI AGTACT 1 cut(s) 2646
Bme1390I CCNGG 8 cut(s) 206, 334, 367, 681, 963, 2040, 2828, 3063
Bme18I GGWCC 3 cut(s) 184, 346, 3301
BmeRI GACNNNNNGTC 1 cut(s) 3238
BmeT110I CYCGRG 2 cut(s) 187, 214
BmgBI CACGTC 1 cut(s) 2150
BmgT120I GGNCC 5 cut(s) 184, 346, 1462, 3158, 3301
BmiI GGNNCC 8 cut(s) 84, 186, 347, 501, 1463, 2044, 2220, 3060
BmrFI CCNGG 8 cut(s) 206, 334, 367, 681, 963, 2040, 2828, 3063
BmsI GCATC 6 cut(s) 1212, 1254, 1366, 1431, 1462, 1726
BoxI GACNNNNGTC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 2552
BplI GAGNNNNNCTC 7 cut(s) 31, 507, 539, 567, 599, 929, 961
BpmI CTGGAG 3 cut(s) 311, 349, 2022
Bpu10I CCTNAGC 1 cut(s) 1797
BpuEI CTTGAG 2 cut(s) 1580, 1847
BpuMI CCSGG 4 cut(s) 206, 334, 681, 3063
BsaAI YACGTR 1 cut(s) 232
BsaBI GATNNNNATC 3 cut(s) 389, 927, 1202
BsaHI GRCGYC 3 cut(s) 83, 119, 155
BsaI GGTCTC 5 cut(s) 41, 609, 874, 1664, 2460
BsaJI CCNNGG 8 cut(s) 6, 78, 89, 188, 505, 915, 1167, 1654
BsaWI WCCGGW 4 cut(s) 661, 1859, 2062, 2133
Bsc4I CCNNNNNNNGG 9 cut(s) 324, 339, 569, 700, 1194, 1252, 1278, 2665, 2775
Bse118I RCCGGY 4 cut(s) 100, 253, 562, 1859
Bse1I ACTGG 3 cut(s) 839, 2456, 2979
Bse3DI GCAATG 1 cut(s) 349
Bse8I GATNNNNATC 3 cut(s) 389, 927, 1202
BseAI TCCGGA 2 cut(s) 661, 2062
BseBI CCWGG 4 cut(s) 367, 963, 2040, 2828
BseDI CCNNGG 8 cut(s) 6, 78, 89, 188, 505, 915, 1167, 1654
BseGI GGATG 6 cut(s) 147, 210, 390, 925, 1381, 1446
BseJI GATNNNNATC 3 cut(s) 389, 927, 1202
BseLI CCNNNNNNNGG 9 cut(s) 324, 339, 569, 700, 1194, 1252, 1278, 2665, 2775
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 9 cut(s) 473, 620, 1070, 1659, 1707, 1811, 1824, 3062, 3069
BseNI ACTGG 3 cut(s) 839, 2456, 2979
BseRI GAGGAG 2 cut(s) 23, 3272
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 4 cut(s) 365, 1462, 1834, 2897
BshFI GGCC 8 cut(s) 5, 94, 1187, 1464, 1925, 2015, 2728, 3160
BshNI GGYRCC 2 cut(s) 82, 2218
BshTI ACCGGT 1 cut(s) 1859
BsiHKAI GWGCWC 3 cut(s) 237, 365, 1596
BsiHKCI CYCGRG 2 cut(s) 187, 214
BslFI GGGAC 1 cut(s) 1227
BslI CCNNNNNNNGG 9 cut(s) 324, 339, 569, 700, 1194, 1252, 1278, 2665, 2775
BsmAI GTCTC 9 cut(s) 41, 162, 609, 874, 1327, 1556, 1664, 2274, 2460
BsmBI CGTCTC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 1227
BsmI GAATGC 2 cut(s) 1066, 2558
BsnI GGCC 8 cut(s) 5, 94, 1187, 1464, 1925, 2015, 2728, 3160
Bso31I GGTCTC 5 cut(s) 41, 609, 874, 1664, 2460
BsoBI CYCGRG 2 cut(s) 187, 214
Bsp1286I GDGCHC 3 cut(s) 237, 365, 1596
Bsp13I TCCGGA 2 cut(s) 661, 2062
Bsp1407I TGTACA 2 cut(s) 1852, 2527
Bsp143I GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
BspANI GGCC 8 cut(s) 5, 94, 1187, 1464, 1925, 2015, 2728, 3160
BspCNI CTCAG 9 cut(s) 472, 619, 1069, 1660, 1706, 1810, 1823, 3061, 3070
BspEI TCCGGA 2 cut(s) 661, 2062
BspHI TCATGA 1 cut(s) 2704
BspLI GGNNCC 8 cut(s) 84, 186, 347, 501, 1463, 2044, 2220, 3060
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 3 cut(s) 78, 200, 1085
BspT107I GGYRCC 2 cut(s) 82, 2218
BspTNI GGTCTC 5 cut(s) 41, 609, 874, 1664, 2460
BsrDI GCAATG 1 cut(s) 349
BsrFI RCCGGY 4 cut(s) 100, 253, 562, 1859
BsrGI TGTACA 2 cut(s) 1852, 2527
BsrI ACTGG 3 cut(s) 839, 2456, 2979
BssAI RCCGGY 4 cut(s) 100, 253, 562, 1859
BssECI CCNNGG 8 cut(s) 6, 78, 89, 188, 505, 915, 1167, 1654
BssMI GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
BssNAI GTATAC 1 cut(s) 1878
BssNI GRCGYC 3 cut(s) 83, 119, 155
BssSI CACGAG 1 cut(s) 2570
BssT1I CCWWGG 3 cut(s) 505, 915, 1167
Bst1107I GTATAC 1 cut(s) 1878
Bst2BI CACGAG 1 cut(s) 2570
Bst2UI CCWGG 4 cut(s) 367, 963, 2040, 2828
Bst4CI ACNGT 7 cut(s) 35, 651, 895, 2128, 2551, 3014, 3305
Bst6I CTCTTC 4 cut(s) 877, 1758, 2356, 2463
BstACI GRCGYC 3 cut(s) 83, 119, 155
BstAPI GCANNNNNTGC 1 cut(s) 2891
BstAUI TGTACA 2 cut(s) 1852, 2527
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 255, 2733
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 6 cut(s) 147, 210, 390, 925, 1381, 1446
BstH2I RGCGCY 2 cut(s) 86, 260
BstHHI GCGC 4 cut(s) 85, 143, 259, 2797
BstKTI GATC 8 cut(s) 73, 195, 931, 1093, 1576, 1719, 2961, 3221
BstMAI GTCTC 9 cut(s) 41, 162, 609, 874, 1327, 1556, 1664, 2274, 2460
BstMBI GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
BstNI CCWGG 4 cut(s) 367, 963, 2040, 2828
BstNSI RCATGY 2 cut(s) 2280, 2900
BstPAI GACNNNNGTC 1 cut(s) 33
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 8 cut(s) 204, 332, 365, 679, 961, 2038, 2826, 3061
BstSFI CTRYAG 1 cut(s) 2508
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 4 cut(s) 365, 1462, 1834, 2897
BstV2I GAAGAC 1 cut(s) 2552
BstXI CCANNNNNNTGG 3 cut(s) 397, 887, 977
BstZ17I GTATAC 1 cut(s) 1878
BsuI GTATCC 2 cut(s) 1948, 2637
BsuRI GGCC 8 cut(s) 5, 94, 1187, 1464, 1925, 2015, 2728, 3160
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 1359
BtrI CACGTC 1 cut(s) 2150
BtsCI GGATG 6 cut(s) 147, 210, 390, 925, 1381, 1446
BtsI GCAGTG 1 cut(s) 2997
BtsIMutI CAGTG 6 cut(s) 891, 2181, 2449, 2967, 2986, 2997
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 2 cut(s) 255, 2733
CaiI CAGNNNCTG 1 cut(s) 372
CciI TCATGA 1 cut(s) 2704
CfoI GCGC 4 cut(s) 85, 143, 259, 2797
Cfr10I RCCGGY 4 cut(s) 100, 253, 562, 1859
Cfr13I GGNCC 5 cut(s) 184, 346, 1462, 3158, 3301
CseI GACGC 4 cut(s) 108, 343, 1349, 1984
Csp6I GTAC 8 cut(s) 169, 691, 1730, 1853, 2219, 2528, 2645, 3018
CspAI ACCGGT 1 cut(s) 1859
CspCI CAANNNNNGTGG 2 cut(s) 1971, 2006
CviQI GTAC 8 cut(s) 169, 691, 1730, 1853, 2219, 2528, 2645, 3018
DinI GGCGCC 1 cut(s) 84
DpnI GATC 8 cut(s) 72, 194, 930, 1092, 1575, 1718, 2960, 3220
DpnII GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
DraI TTTAAA 1 cut(s) 811
DraIII CACNNNGTG 1 cut(s) 2775
DriI GACNNNNNGTC 1 cut(s) 3238
EaeI YGGCCR 3 cut(s) 3, 1185, 2013
Eam1104I CTCTTC 4 cut(s) 877, 1758, 2356, 2463
Eam1105I GACNNNNNGTC 1 cut(s) 3238
EarI CTCTTC 4 cut(s) 877, 1758, 2356, 2463
EciI GGCGGA 1 cut(s) 549
Ecl136II GAGCTC 1 cut(s) 363
Eco130I CCWWGG 3 cut(s) 505, 915, 1167
Eco24I GRGCYC 1 cut(s) 365
Eco31I GGTCTC 5 cut(s) 41, 609, 874, 1664, 2460
Eco32I GATATC 3 cut(s) 180, 1383, 2247
Eco47I GGWCC 3 cut(s) 184, 346, 3301
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 4 cut(s) 1092, 2079, 2455, 2564
Eco72I CACGTG 1 cut(s) 232
Eco88I CYCGRG 2 cut(s) 187, 214
Eco91I GGTNACC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 363
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 3158
EcoO65I GGTNACC 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 2606
EcoRII CCWGG 4 cut(s) 365, 961, 2038, 2826
EcoRV GATATC 3 cut(s) 180, 1383, 2247
EcoT14I CCWWGG 3 cut(s) 505, 915, 1167
EcoT38I GRGCYC 1 cut(s) 365
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 505, 915, 1167
Esp3I CGTCTC 1 cut(s) 162
FalI AAGNNNNNCTT 2 cut(s) 3261, 3293
FaqI GGGAC 1 cut(s) 1227
FauI CCCGC 2 cut(s) 127, 258
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 2 cut(s) 928, 1716
FblI GTMKAC 4 cut(s) 27, 198, 432, 1877
FokI GGATG 6 cut(s) 134, 197, 377, 932, 1388, 1453
FriOI GRGCYC 1 cut(s) 365
FspBI CTAG 5 cut(s) 419, 762, 1278, 2273, 2564
GlaI GCGC 4 cut(s) 84, 142, 258, 2796
GsuI CTGGAG 3 cut(s) 311, 349, 2022
HaeII RGCGCY 2 cut(s) 86, 260
HaeIII GGCC 8 cut(s) 5, 94, 1187, 1464, 1925, 2015, 2728, 3160
HgaI GACGC 4 cut(s) 108, 343, 1349, 1984
HhaI GCGC 4 cut(s) 85, 143, 259, 2797
Hin1I GRCGYC 3 cut(s) 83, 119, 155
Hin6I GCGC 4 cut(s) 83, 141, 257, 2795
HinP1I GCGC 4 cut(s) 83, 141, 257, 2795
HincII GTYRAC 5 cut(s) 28, 199, 433, 832, 3138
HindII GTYRAC 5 cut(s) 28, 199, 433, 832, 3138
HindIII AAGCTT 1 cut(s) 2057
HphI GGTGA 8 cut(s) 322, 900, 1235, 2123, 2366, 2609, 2953, 3020
Hpy99I CGWCG 8 cut(s) 29, 32, 35, 124, 127, 157, 200, 2000
HpyCH4III ACNGT 7 cut(s) 35, 651, 895, 2128, 2551, 3014, 3305
HpyCH4IV ACGT 5 cut(s) 155, 231, 2010, 2149, 3129
HpySE526I ACGT 5 cut(s) 155, 231, 2010, 2149, 3129
Hsp92I GRCGYC 3 cut(s) 83, 119, 155
HspAI GCGC 4 cut(s) 83, 141, 257, 2795
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 2 cut(s) 661, 2062
KpnI GGTACC 1 cut(s) 2222
KroI GCCGGC 1 cut(s) 253
KroNI GCCGGC 1 cut(s) 255
Ksp22I TGATCA 2 cut(s) 928, 1716
Kzo9I GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
LmnI GCTCC 6 cut(s) 368, 369, 1599, 2042, 2403, 3064
Lsp1109I GCAGC 4 cut(s) 365, 1462, 1834, 2897
LweI GCATC 6 cut(s) 1212, 1254, 1366, 1431, 1462, 1726
MaeI CTAG 5 cut(s) 419, 762, 1278, 2273, 2564
MaeII ACGT 5 cut(s) 155, 231, 2010, 2149, 3129
MalI GATC 8 cut(s) 72, 194, 930, 1092, 1575, 1718, 2960, 3220
MboI GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
MfeI CAATTG 3 cut(s) 470, 1452, 2880
MhlI GDGCHC 3 cut(s) 237, 365, 1596
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 5 cut(s) 585, 1636, 2231, 2695, 3268
MmeI TCCRAC 3 cut(s) 1296, 2556, 2979
MreI CGCCGGCG 1 cut(s) 253
MroI TCCGGA 2 cut(s) 661, 2062
MroNI GCCGGC 1 cut(s) 253
MroXI GAANNNNTTC 3 cut(s) 1411, 2143, 2712
MslI CAYNNNNRTG 4 cut(s) 395, 488, 1860, 2510
MspA1I CMGCKG 2 cut(s) 1697, 2888
MspR9I CCNGG 8 cut(s) 206, 334, 367, 681, 963, 2040, 2828, 3063
MunI CAATTG 3 cut(s) 470, 1452, 2880
Mva1269I GAATGC 2 cut(s) 1066, 2558
MvaI CCWGG 4 cut(s) 367, 963, 2040, 2828
NaeI GCCGGC 1 cut(s) 255
NarI GGCGCC 1 cut(s) 83
NciI CCSGG 4 cut(s) 206, 334, 681, 3063
NdeI CATATG 1 cut(s) 862
NdeII GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
NgoMIV GCCGGC 1 cut(s) 253
NlaIV GGNNCC 8 cut(s) 84, 186, 347, 501, 1463, 2044, 2220, 3060
NmeAIII GCCGAG 2 cut(s) 31, 114
NmuCI GTSAC 9 cut(s) 328, 906, 2279, 2698, 3008, 3073, 3175, 3187, 3271
NspI RCATGY 2 cut(s) 2280, 2900
OliI CACNNNNGTG 1 cut(s) 2510
PagI TCATGA 1 cut(s) 2704
PaqCI CACCTGC 1 cut(s) 231
PciI ACATGT 1 cut(s) 2896
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 2 cut(s) 1066, 2558
PdiI GCCGGC 1 cut(s) 255
PdmI GAANNNNTTC 3 cut(s) 1411, 2143, 2712
PfeI GAWTC 7 cut(s) 1100, 1181, 1540, 1650, 1689, 2257, 2539
PflMI CCANNNNNTGG 2 cut(s) 1194, 2775
PfoI TCCNGGA 5 cut(s) 365, 679, 961, 2038, 3061
PinAI ACCGGT 1 cut(s) 1859
PleI GAGTC 5 cut(s) 584, 1635, 2231, 2695, 3268
PluTI GGCGCC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
PpsI GAGTC 5 cut(s) 584, 1635, 2231, 2695, 3268
Ppu21I YACGTR 1 cut(s) 232
PscI ACATGT 1 cut(s) 2896
PshAI GACNNNNGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 365
Psp6I CCWGG 4 cut(s) 365, 961, 2038, 2826
PspCI CACGTG 1 cut(s) 232
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 4 cut(s) 365, 961, 2038, 2826
PspN4I GGNNCC 8 cut(s) 84, 186, 347, 501, 1463, 2044, 2220, 3060
PspPI GGNCC 5 cut(s) 184, 346, 1462, 3158, 3301
PstNI CAGNNNCTG 1 cut(s) 372
PvuII CAGCTG 2 cut(s) 1697, 2888
RsaI GTAC 8 cut(s) 170, 692, 1731, 1854, 2220, 2529, 2646, 3019
RsaNI GTAC 8 cut(s) 169, 691, 1730, 1853, 2219, 2528, 2645, 3018
RseI CAYNNNNRTG 4 cut(s) 395, 488, 1860, 2510
SacI GAGCTC 1 cut(s) 365
SalI GTCGAC 3 cut(s) 26, 197, 431
Sau3AI GATC 8 cut(s) 70, 192, 928, 1090, 1573, 1716, 2958, 3218
Sau96I GGNCC 5 cut(s) 184, 346, 1462, 3158, 3301
ScaI AGTACT 1 cut(s) 2646
SchI GAGTC 5 cut(s) 585, 1636, 2231, 2695, 3268
ScrFI CCNGG 8 cut(s) 206, 334, 367, 681, 963, 2040, 2828, 3063
SduI GDGCHC 3 cut(s) 237, 365, 1596
SfaNI GCATC 6 cut(s) 1212, 1254, 1366, 1431, 1462, 1726
SfcI CTRYAG 1 cut(s) 2508
SfoI GGCGCC 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 253
SgrDI CGTCGACG 1 cut(s) 26
SinI GGWCC 3 cut(s) 184, 346, 3301
SmiMI CAYNNNNRTG 4 cut(s) 395, 488, 1860, 2510
SmlI CTYRAG 2 cut(s) 1559, 1826
SmoI CTYRAG 2 cut(s) 1559, 1826
SpeI ACTAGT 1 cut(s) 761
SspDI GGCGCC 1 cut(s) 82
SspI AATATT 2 cut(s) 1234, 1711
SspMI CTAG 5 cut(s) 419, 762, 1278, 2273, 2564
SstI GAGCTC 1 cut(s) 365
StyD4I CCNGG 8 cut(s) 204, 332, 365, 679, 961, 2038, 2826, 3061
StyI CCWWGG 3 cut(s) 505, 915, 1167
TaaI ACNGT 7 cut(s) 35, 651, 895, 2128, 2551, 3014, 3305
TaiI ACGT 5 cut(s) 158, 234, 2013, 2152, 3132
TaqII GACCGA 1 cut(s) 2510
TatI WGTACW 5 cut(s) 1729, 1852, 2527, 2644, 3017
TauI GCSGC 9 cut(s) 88, 97, 100, 131, 134, 229, 2018, 2093, 2731
TfiI GAWTC 7 cut(s) 1100, 1181, 1540, 1650, 1689, 2257, 2539
TscAI CASTG 6 cut(s) 898, 2188, 2456, 2974, 2986, 2997
TseFI GTSAC 9 cut(s) 328, 906, 2279, 2698, 3008, 3073, 3175, 3187, 3271
TseI GCWGC 4 cut(s) 378, 1475, 1822, 2885
Tsp45I GTSAC 9 cut(s) 328, 906, 2279, 2698, 3008, 3073, 3175, 3187, 3271
TspGWI ACGGA 1 cut(s) 500
TspRI CASTG 6 cut(s) 898, 2188, 2456, 2974, 2986, 2997
Van91I CCANNNNNTGG 2 cut(s) 1194, 2775
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 3 cut(s) 184, 346, 3301
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 6 cut(s) 744, 2076, 2497, 2516, 2606, 2650
XceI RCATGY 2 cut(s) 2280, 2900
XmiI GTMKAC 4 cut(s) 27, 198, 432, 1877
XmnI GAANNNNTTC 3 cut(s) 1411, 2143, 2712
XspI CTAG 5 cut(s) 419, 762, 1278, 2273, 2564
ZraI GACGTC 1 cut(s) 156
ZrmI AGTACT 1 cut(s) 2646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.