Rorug04G0121800

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017772
Physical Location & Seq
Forward (+)
19319594 .. 19319877
284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0121800.1

Sequence Viewer

Length: 192 bp
ATGGTTGGTTGTAAGCTGCATAGGGATCTGGCACACAAAACTGTTCTCAAGTCATTGGTTCTGTTGAAGAATGGAAAGGAGCCAAGAAAACCTTTTCTTCCATTAGGTAGAAAAGCCAAAAGAATATTGGTTACCGGAATGCATGCTGATGATCTTGGATATCAGTGTGGAGGATGGACAGTTAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

63

Amino Acids

6.98

Weight (kDa)

10.11

Isoelectric Point (pI)

22.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3_C PF01915 19 - 61 5.1e-06 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 33
AfaI GTAC 1 cut(s) 187
AgsI TTSAA 1 cut(s) 67
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
AlwI GGATC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 3
BccI CCATC 1 cut(s) 168
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 32
BsaWI WCCGGW 1 cut(s) 134
BseGI GGATG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 3
BsiSI CCGG 1 cut(s) 135
BsmI GAATGC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 25, 151
BspLI GGNNCC 1 cut(s) 81
BspPI GGATC 1 cut(s) 33
BssMI GATC 2 cut(s) 25, 151
Bst4CI ACNGT 2 cut(s) 43, 181
BstC8I GCNNGC 1 cut(s) 144
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 2 cut(s) 28, 154
BstMBI GATC 2 cut(s) 25, 151
BstNSI RCATGY 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 130
BstV1I GCAGC 1 cut(s) 3
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BtsCI GGATG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 186
CviAII CATG 1 cut(s) 143
CviJI RGCY 3 cut(s) 16, 82, 116
CviKI_1 RGCY 3 cut(s) 16, 82, 116
CviQI GTAC 1 cut(s) 186
DpnI GATC 2 cut(s) 27, 153
DpnII GATC 2 cut(s) 25, 151
Eco32I GATATC 1 cut(s) 161
Eco91I GGTNACC 1 cut(s) 130
EcoO65I GGTNACC 1 cut(s) 130
EcoRV GATATC 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 144
FaeI CATG 1 cut(s) 146
FaiI YATR 2 cut(s) 21, 144
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 17
GluI GCNGC 1 cut(s) 17
HapII CCGG 1 cut(s) 135
Hin1II CATG 1 cut(s) 146
HpaII CCGG 1 cut(s) 135
HpyCH4III ACNGT 2 cut(s) 43, 181
HpyCH4V TGCA 2 cut(s) 19, 142
Hsp92II CATG 1 cut(s) 146
Kzo9I GATC 2 cut(s) 25, 151
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 2 cut(s) 14, 148
Lsp1109I GCAGC 1 cut(s) 3
MaeIII GTNAC 1 cut(s) 130
MalI GATC 2 cut(s) 27, 153
MboI GATC 2 cut(s) 25, 151
MboII GAAGA 2 cut(s) 79, 89
MflI RGATCY 1 cut(s) 25
MnlI CCTC 1 cut(s) 164
Mph1103I ATGCAT 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 147
MspI CCGG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 144
NdeII GATC 2 cut(s) 25, 151
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 144
NspI RCATGY 1 cut(s) 146
PaeI GCATGC 1 cut(s) 146
PctI GAATGC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 18
PspEI GGTNACC 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 81
PsuI RGATCY 1 cut(s) 25
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 1 cut(s) 147
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 2 cut(s) 25, 151
ScaI AGTACT 1 cut(s) 187
SetI ASST 3 cut(s) 18, 94, 109
SgeI CNNG 6 cut(s) 41, 61, 96, 147, 155, 167
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
SphI GCATGC 1 cut(s) 146
SspI AATATT 1 cut(s) 126
TaaI ACNGT 2 cut(s) 43, 181
TatI WGTACW 1 cut(s) 185
TscAI CASTG 1 cut(s) 170
TseI GCWGC 1 cut(s) 16
TspRI CASTG 1 cut(s) 170
XceI RCATGY 1 cut(s) 146
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 187
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.