Rorug02G0173200

RNA-binding protein 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008166
Physical Location & Seq
Reverse (-)
15744046 .. 15744897
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0173200.1

Sequence Viewer

Length: 561 bp
ATGAAAAGCAGTGGGAGTAAAATGCCCAAGGAGCAGGAGCACGCTATCATTGTCTCCACCCTGAAACGGGTAATCCGCGGTGGCGTTGGCACAGACAATGCACCCTCCCTGCAGCCACCGCCAGCAACGGGTGGCACCGATCAATTCGTGATACCATTTTCGGATTGTGATGACACGTGTCAGGTATGTAAGATGGGGAAGGACGATTGCCTTGGCTGTGGTTTGTTCCCGCCAAGCGAGCAGGACAAAGGTAAAAAGGTGAAGACGAGCAAGTACAGGGGAGTGCGGCAGAGACCAGGAGGAAAATGGGCTGCGGAGATTAGGGACCCGCATCTCGCCGTTCGGCTTTGGCTCGGGACTTTCCAGACGGAGGAGGAGGCAGCCAGGGCTTATGACACGAAGGCCCTCGAGCTTCGAGGAGCTGACAGAGCCAAGCTAAACTTCCCGCCATCCTCGGACACTGGTACTACCAGTGGAGCAATGGCTGATGAGACCAAAGTCCAAAAAATTCCAGTCGGAGAGAGCAGCGCTGCTGAGGAAGAAGAAGAGATGGATCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.08

Weight (kDa)

5.54

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 90 - 140 1.8e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 78
AciI CCGC 8 cut(s) 76, 78, 119, 230, 286, 314, 329, 446
AclWI GGATC 1 cut(s) 548
AcsI RAATTY 1 cut(s) 507
AcvI CACGTG 1 cut(s) 177
AfaI GTAC 2 cut(s) 275, 466
AfeI AGCGCT 1 cut(s) 529
AfiI CCNNNNNNNGG 4 cut(s) 66, 67, 128, 370
AflIII ACRYGT 2 cut(s) 174, 176
AjnI CCWGG 2 cut(s) 295, 383
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 3 cut(s) 412, 422, 436
AluI AGCT 3 cut(s) 412, 422, 436
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 3 cut(s) 58, 286, 485
AlwI GGATC 1 cut(s) 548
Ama87I CYCGRG 2 cut(s) 353, 407
Aor51HI AGCGCT 1 cut(s) 529
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 5 cut(s) 112, 311, 380, 525, 530
ApoI RAATTY 1 cut(s) 507
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 2 cut(s) 325, 403
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 2 cut(s) 353, 407
AvaII GGWCC 1 cut(s) 325
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 134
BbrPI CACGTG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 5 cut(s) 124, 298, 392, 517, 537
BccI CCATC 3 cut(s) 187, 457, 544
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 2 cut(s) 297, 385
BcoDI GTCTC 3 cut(s) 58, 286, 485
BfmI CTRYAG 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 531
BisI GCNGC 6 cut(s) 113, 287, 312, 381, 526, 531
BlsI GCNGC 6 cut(s) 114, 288, 313, 382, 527, 532
Bme1390I CCNGG 2 cut(s) 297, 385
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 2 cut(s) 353, 407
BmgT120I GGNCC 2 cut(s) 325, 403
BmiI GGNNCC 4 cut(s) 136, 326, 327, 555
BmrFI CCNGG 2 cut(s) 297, 385
BmsI GCATC 1 cut(s) 340
BoxI GACNNNNGTC 2 cut(s) 177, 497
BpiI GAAGAC 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 286, 485
BsaJI CCNNGG 5 cut(s) 27, 76, 211, 384, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 67, 128, 370
Bse1I ACTGG 3 cut(s) 466, 471, 512
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 2 cut(s) 297, 385
BseDI CCNNGG 5 cut(s) 27, 76, 211, 384, 453
BseGI GGATG 1 cut(s) 449
BseLI CCNNNNNNNGG 4 cut(s) 66, 67, 128, 370
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 3 cut(s) 466, 471, 512
BseRI GAGGAG 3 cut(s) 386, 389, 432
BseXI GCAGC 5 cut(s) 124, 298, 392, 517, 537
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 404
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 2 cut(s) 353, 407
BslFI GGGAC 2 cut(s) 338, 370
BslI CCNNNNNNNGG 4 cut(s) 66, 67, 128, 370
BsmAI GTCTC 3 cut(s) 58, 286, 485
BsmFI GGGAC 2 cut(s) 338, 370
BsnI GGCC 1 cut(s) 404
Bso31I GGTCTC 2 cut(s) 286, 485
BsoBI CYCGRG 2 cut(s) 353, 407
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 139, 553
BspACI CCGC 8 cut(s) 76, 78, 119, 230, 286, 314, 329, 446
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 136, 326, 327, 555
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 2 cut(s) 286, 485
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 3 cut(s) 466, 471, 512
BssECI CCNNGG 5 cut(s) 27, 76, 211, 384, 453
BssMI GATC 2 cut(s) 139, 553
BssT1I CCWWGG 2 cut(s) 27, 211
Bst2UI CCWGG 2 cut(s) 297, 385
Bst6I CTCTTC 1 cut(s) 540
BstBAI YACGTR 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 42, 123, 239
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 449
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 2 cut(s) 142, 556
BstMAI GTCTC 3 cut(s) 58, 286, 485
BstMBI GATC 2 cut(s) 139, 553
BstMWI GCNNNNNNNGC 5 cut(s) 31, 118, 238, 386, 428
BstNI CCWGG 2 cut(s) 297, 385
BstPAI GACNNNNGTC 2 cut(s) 177, 497
BstSCI CCNGG 2 cut(s) 295, 383
BstSFI CTRYAG 1 cut(s) 110
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 124, 298, 392, 517, 537
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 1 cut(s) 404
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 449
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 459, 478
Cac8I GCNNGC 3 cut(s) 42, 123, 239
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 325, 403
Cfr42I CCGCGG 1 cut(s) 79
Csp6I GTAC 2 cut(s) 274, 465
CviQI GTAC 2 cut(s) 274, 465
DdeI CTNAG 1 cut(s) 534
DpnI GATC 2 cut(s) 141, 555
DpnII GATC 2 cut(s) 139, 553
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco130I CCWWGG 2 cut(s) 27, 211
Eco31I GGTCTC 2 cut(s) 286, 485
Eco47I GGWCC 1 cut(s) 325
Eco47III AGCGCT 1 cut(s) 529
Eco72I CACGTG 1 cut(s) 177
Eco88I CYCGRG 2 cut(s) 353, 407
EcoO109I RGGNCCY 2 cut(s) 325, 403
EcoRII CCWGG 2 cut(s) 295, 383
EcoT14I CCWWGG 2 cut(s) 27, 211
ErhI CCWWGG 2 cut(s) 27, 211
FaiI YATR 2 cut(s) 187, 393
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 2 cut(s) 338, 370
FauI CCCGC 3 cut(s) 237, 336, 453
Fnu4HI GCNGC 6 cut(s) 113, 287, 312, 381, 526, 531
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 6 cut(s) 113, 287, 312, 381, 526, 531
GlaI GCGC 1 cut(s) 529
GluI GCNGC 6 cut(s) 113, 287, 312, 381, 526, 531
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 1 cut(s) 404
HhaI GCGC 1 cut(s) 530
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 3 cut(s) 163, 457, 518
Hpy188III TCNNGA 3 cut(s) 148, 355, 364
HpyAV CCTTC 2 cut(s) 193, 394
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 101, 112
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 118, 238, 386, 428
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 1 cut(s) 176
HspAI GCGC 1 cut(s) 528
KflI GGGWCCC 1 cut(s) 325
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 2 cut(s) 139, 553
LmnI GCTCC 4 cut(s) 31, 37, 419, 476
Lsp1109I GCAGC 5 cut(s) 124, 298, 392, 517, 537
LweI GCATC 1 cut(s) 340
MaeII ACGT 1 cut(s) 176
MalI GATC 2 cut(s) 141, 555
MboI GATC 2 cut(s) 139, 553
MboII GAAGA 4 cut(s) 274, 551, 554, 557
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 2 cut(s) 143, 507
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 9 cut(s) 115, 293, 364, 367, 370, 410, 416, 463, 529
MspA1I CMGCKG 1 cut(s) 78
MspR9I CCNGG 2 cut(s) 297, 385
MvaI CCWGG 2 cut(s) 297, 385
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 5 cut(s) 31, 118, 238, 386, 428
NdeII GATC 2 cut(s) 139, 553
NlaIV GGNNCC 4 cut(s) 136, 326, 327, 555
PaeR7I CTCGAG 1 cut(s) 407
PkrI GCNGC 6 cut(s) 114, 288, 313, 382, 527, 532
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 325
PshAI GACNNNNGTC 2 cut(s) 177, 497
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 295, 383
PspCI CACGTG 1 cut(s) 177
PspGI CCWGG 2 cut(s) 295, 383
PspN4I GGNNCC 4 cut(s) 136, 326, 327, 555
PspPI GGNCC 2 cut(s) 325, 403
PspPPI RGGWCCY 1 cut(s) 325
PspXI VCTCGAGB 1 cut(s) 407
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 2 cut(s) 275, 466
RsaNI GTAC 2 cut(s) 274, 465
SacII CCGCGG 1 cut(s) 79
SatI GCNGC 6 cut(s) 113, 287, 312, 381, 526, 531
Sau3AI GATC 2 cut(s) 139, 553
Sau96I GGNCC 2 cut(s) 325, 403
ScrFI CCNGG 2 cut(s) 297, 385
SduI GDGCHC 1 cut(s) 42
SetI ASST 7 cut(s) 179, 186, 253, 261, 414, 424, 438
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 110
Sfr274I CTCGAG 1 cut(s) 407
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 1 cut(s) 325
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 2 cut(s) 143, 507
SsiI CCGC 8 cut(s) 76, 78, 119, 230, 286, 314, 329, 446
StyD4I CCNGG 2 cut(s) 295, 383
StyI CCWWGG 2 cut(s) 27, 211
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 408, 415
TasI AATT 2 cut(s) 143, 507
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 289
TscAI CASTG 3 cut(s) 16, 466, 478
TseI GCWGC 5 cut(s) 112, 311, 380, 525, 530
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 383, 546
TspRI CASTG 3 cut(s) 16, 466, 478
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 2 cut(s) 303, 478
XhoI CTCGAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.