Rorug02G0359800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010155
Physical Location & Seq
Reverse (-)
45574038 .. 45575005
968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0359800.1

Sequence Viewer

Length: 864 bp
ATGGAGATTCTATCATGGAATATCAGGGGTACCAAAGACCCTAAGAAATTCAGGGCTTTGGCGTCGATTATTCAGCAGAGACGTCCTAGTATTGTGTTCTGGATCGAGACAAAGTGTAGAGACAAGAAAGCATTCGAACGGTTCCGCAACGATTTGGGTTTCCGTCATGGCATAATGCAAGAGTGTTCTGATACAGGTGGAGGAGGTTTGATGCTATTGTGGGTGGATGAAATTAATCTTCATCTCCGGTCGCACTCACCGCATCACATTGATGTAGATGTTGTGCTGCCGGAATCTGAGGGAGGGAGATGGCGACTCACAGGTTTTTATGGGCATCCAAAAGCTCATCTCCGCTCTGCGTCATGGGATTTGTTGCGTCTGCTTGCCTTCATGCCAGGTTCTCCTAACTGGTTGGTGGTTGGTGATCTAAATGAAATCATCAGCTCGAGGGAGAAATCAGGAGGAGGTCTTCGTCCCTCAAGGCAAATGGATGCGTTCCAAACAGTATTGGATGACTGTACTCTTACAGATTTAGGGTTTGAGGGCAGTCTATTCACATGGCATAATTCAGAGACTCAATGTAGATTAGACCGTGCAGTTGCCACGCAGGGTTGGTGTCAAGTGTATGGACTGAATAGGGTGCTCCACCTTCCGCCCTCTTGTTCTGATCATGTCCCGATTCTTGTTGAAGCACATATTCAGGCTCCAATTCAGTTTCCATCACGCAGAACCAGATTTCGGTTTGAAGAGATGTGGACGAAGGATGATTCTTGTGAAGAAGTGATCCGGGAGAAGTGGCAGACGGATAGGGATGGTTTCGAGCAGTTTCGGTTGCAGGGAAAACTTTATAGCACGAGTGTCTGA

Protein Analysis

287

Amino Acids

33.14

Weight (kDa)

7.17

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 4 - 224 5.3e-15 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 85
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 354
AciI CCGC 4 cut(s) 145, 260, 352, 653
AclWI GGATC 2 cut(s) 110, 778
AcsI RAATTY 1 cut(s) 47
AcyI GRCGYC 2 cut(s) 62, 82
AfaI GTAC 2 cut(s) 31, 520
AfiI CCNNNNNNNGG 1 cut(s) 738
AgsI TTSAA 2 cut(s) 689, 746
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 2 cut(s) 344, 444
AluI AGCT 2 cut(s) 344, 444
Alw21I GWGCWC 1 cut(s) 645
Alw26I GTCTC 4 cut(s) 73, 101, 114, 566
AlwI GGATC 2 cut(s) 110, 778
Ama87I CYCGRG 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 47
AseI ATTAAT 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 131
Asp718I GGTACC 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 2 cut(s) 249, 434
AsuII TTCGAA 1 cut(s) 135
AvaI CYCGRG 1 cut(s) 445
BanI GGYRCC 1 cut(s) 29
BauI CACGAG 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 645
BbvI GCAGC 1 cut(s) 273
BccI CCATC 3 cut(s) 303, 727, 806
BciT130I CCWGG 1 cut(s) 396
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 4 cut(s) 73, 101, 114, 566
BfaI CTAG 1 cut(s) 87
BisI GCNGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 288
Bme1390I CCNGG 2 cut(s) 396, 788
BmeT110I CYCGRG 1 cut(s) 445
BmiI GGNNCC 3 cut(s) 31, 143, 705
BmrFI CCNGG 2 cut(s) 396, 788
BmsI GCATC 4 cut(s) 201, 271, 343, 481
BpiI GAAGAC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 463
BpuMI CCSGG 1 cut(s) 788
BsaHI GRCGYC 2 cut(s) 62, 82
BsaWI WCCGGW 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 738
Bse1I ACTGG 1 cut(s) 413
BseBI CCWGG 1 cut(s) 396
BseGI GGATG 6 cut(s) 232, 334, 496, 517, 769, 817
BseLI CCNNNNNNNGG 1 cut(s) 738
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 216, 477
BseXI GCAGC 1 cut(s) 273
BsgI GTGCAG 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 3 cut(s) 247, 290, 787
BslFI GGGAC 2 cut(s) 459, 659
BslI CCNNNNNNNGG 1 cut(s) 738
BsmAI GTCTC 4 cut(s) 73, 101, 114, 566
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 2 cut(s) 459, 659
BsmI GAATGC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 445
Bsp119I TTCGAA 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 645
Bsp143I GATC 4 cut(s) 102, 424, 667, 783
BspACI CCGC 4 cut(s) 145, 260, 352, 653
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 31, 143, 705
BspPI GGATC 2 cut(s) 110, 778
BspT104I TTCGAA 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 354
BsrI ACTGG 1 cut(s) 413
BssMI GATC 4 cut(s) 102, 424, 667, 783
BssNI GRCGYC 2 cut(s) 62, 82
BssSI CACGAG 1 cut(s) 853
Bst2BI CACGAG 1 cut(s) 853
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 4 cut(s) 141, 505, 518, 593
Bst6I CTCTTC 1 cut(s) 741
BstACI GRCGYC 2 cut(s) 62, 82
BstBI TTCGAA 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 42, 297
BstF5I GGATG 6 cut(s) 232, 334, 496, 517, 769, 817
BstKTI GATC 4 cut(s) 105, 427, 670, 786
BstMAI GTCTC 4 cut(s) 73, 101, 114, 566
BstMBI GATC 4 cut(s) 102, 424, 667, 783
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 2 cut(s) 394, 786
BstV1I GCAGC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 461
BtsCI GGATG 6 cut(s) 232, 334, 496, 517, 769, 817
Cac8I GCNNGC 1 cut(s) 384
CseI GACGC 3 cut(s) 51, 348, 365
Csp6I GTAC 2 cut(s) 30, 519
CviAII CATG 6 cut(s) 15, 167, 363, 391, 558, 671
CviJI RGCY 4 cut(s) 56, 344, 444, 704
CviKI_1 RGCY 4 cut(s) 56, 344, 444, 704
CviQI GTAC 2 cut(s) 30, 519
DdeI CTNAG 2 cut(s) 42, 297
DpnI GATC 4 cut(s) 104, 426, 669, 785
DpnII GATC 4 cut(s) 102, 424, 667, 783
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
EciI GGCGGA 1 cut(s) 642
Eco88I CYCGRG 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 394
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 6 cut(s) 18, 170, 366, 394, 561, 674
FaqI GGGAC 2 cut(s) 459, 659
FatI CATG 6 cut(s) 14, 166, 362, 390, 557, 670
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 6 cut(s) 239, 321, 503, 524, 776, 824
Fsp4HI GCNGC 1 cut(s) 287
FspBI CTAG 1 cut(s) 87
GluI GCNGC 1 cut(s) 287
HapII CCGG 3 cut(s) 247, 290, 787
HgaI GACGC 3 cut(s) 51, 348, 365
Hin1I GRCGYC 2 cut(s) 62, 82
Hin1II CATG 6 cut(s) 18, 170, 366, 394, 561, 674
HinfI GANTC 6 cut(s) 7, 293, 315, 574, 679, 767
HpaII CCGG 3 cut(s) 247, 290, 787
HphI GGTGA 2 cut(s) 249, 434
Hpy166II GTNNAC 1 cut(s) 756
Hpy188I TCNGA 5 cut(s) 190, 298, 571, 667, 863
Hpy188III TCNNGA 4 cut(s) 100, 106, 459, 676
Hpy8I GTNNAC 1 cut(s) 756
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 3 cut(s) 397, 659, 754
HpyCH4III ACNGT 4 cut(s) 141, 505, 518, 593
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 3 cut(s) 178, 596, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 2 cut(s) 42, 297
HpySE526I ACGT 1 cut(s) 82
Hsp92I GRCGYC 2 cut(s) 62, 82
Hsp92II CATG 6 cut(s) 18, 170, 366, 394, 561, 674
KpnI GGTACC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 4 cut(s) 102, 424, 667, 783
LmnI GCTCC 2 cut(s) 648, 709
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 4 cut(s) 201, 271, 343, 481
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 82
MalI GATC 4 cut(s) 104, 426, 669, 785
MbiI CCGCTC 1 cut(s) 354
MboI GATC 4 cut(s) 102, 424, 667, 783
MboII GAAGA 4 cut(s) 230, 461, 758, 788
MhlI GDGCHC 1 cut(s) 645
MluCI AATT 4 cut(s) 47, 231, 565, 708
MlyI GAGTC 2 cut(s) 309, 568
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 3 cut(s) 247, 290, 787
MspR9I CCNGG 2 cut(s) 396, 788
Mva1269I GAATGC 1 cut(s) 131
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 259
NciI CCSGG 1 cut(s) 788
NdeII GATC 4 cut(s) 102, 424, 667, 783
NlaIII CATG 6 cut(s) 18, 170, 366, 394, 561, 674
NlaIV GGNNCC 3 cut(s) 31, 143, 705
NspV TTCGAA 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 445
PctI GAATGC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 4 cut(s) 7, 293, 679, 767
PfoI TCCNGGA 1 cut(s) 786
PkrI GCNGC 1 cut(s) 288
PleI GAGTC 2 cut(s) 309, 568
PpsI GAGTC 2 cut(s) 309, 568
PshBI ATTAAT 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 3 cut(s) 31, 143, 705
PspXI VCTCGAGB 1 cut(s) 445
RsaI GTAC 2 cut(s) 31, 520
RsaNI GTAC 2 cut(s) 30, 519
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 287
Sau3AI GATC 4 cut(s) 102, 424, 667, 783
SchI GAGTC 2 cut(s) 309, 568
ScrFI CCNGG 2 cut(s) 396, 788
SduI GDGCHC 1 cut(s) 645
SetI ASST 9 cut(s) 85, 199, 208, 325, 346, 400, 446, 469, 651
SfaNI GCATC 4 cut(s) 201, 271, 343, 481
Sfr274I CTCGAG 1 cut(s) 445
SfuI TTCGAA 1 cut(s) 135
SlaI CTCGAG 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 2 cut(s) 445, 478
SmoI CTYRAG 2 cut(s) 445, 478
Sse9I AATT 4 cut(s) 47, 231, 565, 708
SsiI CCGC 4 cut(s) 145, 260, 352, 653
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 394, 786
TaaI ACNGT 4 cut(s) 141, 505, 518, 593
TaiI ACGT 1 cut(s) 85
TaqI TCGA 5 cut(s) 65, 105, 135, 446, 819
TasI AATT 4 cut(s) 47, 231, 565, 708
TatI WGTACW 1 cut(s) 518
TfiI GAWTC 4 cut(s) 7, 293, 679, 767
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseI GCWGC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 230, 243, 379, 447
TspGWI ACGGA 2 cut(s) 152, 818
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 87
ZraI GACGTC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.