Rh2DG429300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010155
Physical Location & Seq
Reverse (-)
62037707 .. 62044977
7271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG429300.1

Sequence Viewer

Length: 1686 bp
ATGGTTGAGCGTACAGCTGTAACATGGACAATATTGATAGGTGGGTATTCGCAAGCTGGTCAATACCGTGAAGCATTTAAACTTTACGCTGAGATGCATAGGTGGGGAACTAAGCCAGATTATGTGACTTTTGCAACTCTCTTATCTGGATGCAGCAACATGGATGCTACAAAGGAAGTAGTTCAGGTTCATTCTCATGTCCTTAAATTGGGATATCATTCTACACTCATGGTCTGCAATTCTCTGGTTGATTCCTACTGCAAATCACATTGCCTGGATTTTGCTTTTCGGCTTTTCAAGGAAATGCCTGAAAGAGATGCTGTTACATTCAATGCATTGATAACAGGATACTCGAAAGATGGGTTGAATGAAGGTGCTATTAACCTCTTTGCTGAAATGCAAACTCTGGGTTACAAGCCTTCTGAATTTACATTTGCAGCAGTACTTTGTGCCGGTATCGGTTTAGATGACATTGTGCTTGGGCAACAGGTTCATGGTTTTGTGGTTAAGACTAATTTTGTTTGGAATGTTTTTGTGGGAAATGCATTGCTCGATTTCTACTCAAAGCATGACTATGTGGTTGAAGCAAGGAAACTTTTTGATGAGATGCCAGAGTTGGATTGTATTTCTTACAATATAATCATCTCCTCCTATGTATGGGATGGGCAATTTAAAGAGGCTCTTGATCTTTTCCGTGAATTGCAGCTTACCAAATATGACCGGAAGCAATTCCCTTTTGCAACCATGTTGAGCATAGCAGCGAATACTCTGAACATAAATGTGGGCCGGCAGATACATTCCCAGGCCATTGTAGCAACAGCTGATTCAGAATCTCAGGTTGGAAATTCTTTGGTTGACATGTATGGCAAATGTGGCAGATATACTGAAGCCAGAGGTATATTTGCTAGTCTGGCTGACAGAAGTACAGTGCCATGGACTGCACTCATCTCAGCATATGTTCAGAAGGGACTCCATGAAGAAGGCCTGGAACTGTACAATGAGATGCGCAGAGGAGATGTAACACCATTTTTGAATCTGGATATGGAGTTATTGCATGCAACTAGCAAGGGCCTGTTCATGACACCAATATTATTATTTGAAAAAATATGCCTCAGTGTTCTGCCCATCCAGCCATCTCTTCTCATAGTTGAACAGGACAGACAAATAGTGGAGATATGCTACTTTTCTGCAACATATAATGTCCCCAGAGGATGTTGCCCAGATTGCCTACTATCACTGACGATGGTATCAAACATTGGGGTTGAGGTGGTAAAACCCAGAGCACTCCGCCAAAGCCCTTTCTCCCAGTTCGCAGTCTCGACTGTTCTTCTTGGCAGCTTACCAGCTCTCAAACAGAAGATGGCTTGGAGGCAATTACTGTTCCACACCAGAGCAATTTTGACTCCACATTCAACAACTGGGCTCGCCAGCTTCTCCACCAAATCAACCCCATACTTGGAGAAAGTCGGGATACCCGAATTTTTAAAAGGAATAGGCAATGGGGTGGAAGCTCATGTGGCTAAAATTGAATCTGAGATTGGTGACTTTCAGAAGCTTCTCATCACTCGTACTCTCAGACTGAAGAAACTTGGCATCCCTGTCAAACAAAGGAAGCTGATCTTGAAATACACCCACAAGTATAGGCTGGGACTCTGGAGGCCTCTAGCTCAGCCAGTGAAATCCTAG

Protein Analysis

561

Amino Acids

63.01

Weight (kDa)

8.44

Isoelectric Point (pI)

29.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 5 - 53 8.9e-16 PPR repeat family
PPR PF01535 7 - 36 2.4e-08 PPR repeat
PPR_1 PF12854 105 - 134 9.3e-06 PPR repeat
PPR_2 PF13041 106 - 149 1.3e-10 PPR repeat family
PPR PF01535 108 - 137 1.5e-06 PPR repeat
PPR_2 PF13041 308 - 342 7e-06 PPR repeat family
PPR PF01535 311 - 337 2.6e-07 PPR repeat
SAM_Ribosomal_mS41 PF09597 484 - 550 1.7e-17 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1007
AciI CCGC 1 cut(s) 1288
AcsI RAATTY 3 cut(s) 425, 844, 1478
AcuI CTGAAG 2 cut(s) 906, 1601
AfaI GTAC 5 cut(s) 13, 444, 925, 995, 1570
AfiI CCNNNNNNNGG 3 cut(s) 208, 657, 1456
AflIII ACRYGT 1 cut(s) 858
AjnI CCWGG 3 cut(s) 273, 801, 984
AjuI GAANNNNNNNTTGG 4 cut(s) 822, 854, 1521, 1553
Alw21I GWGCWC 1 cut(s) 1285
Alw26I GTCTC 1 cut(s) 1321
AoxI GGCC 5 cut(s) 784, 804, 982, 1069, 1658
ApeKI GCWGC 5 cut(s) 153, 437, 703, 758, 1335
ApoI RAATTY 3 cut(s) 425, 844, 1478
Asp700I GAANNNNTTC 2 cut(s) 180, 728
AspLEI GCGC 1 cut(s) 1008
AspS9I GGNCC 2 cut(s) 784, 1069
AsuHPI GGTGA 1 cut(s) 1553
BaeI ACNNNNGTAYC 2 cut(s) 1230, 1263
BanII GRGCYC 1 cut(s) 1425
Bbv12I GWGCWC 1 cut(s) 1285
BbvI GCAGC 5 cut(s) 165, 449, 715, 770, 1347
BccI CCATC 6 cut(s) 353, 656, 1133, 1141, 1237, 1354
BciT130I CCWGG 3 cut(s) 275, 803, 986
BciVI GTATCC 2 cut(s) 341, 1464
BcoDI GTCTC 1 cut(s) 1321
BfaI CTAG 4 cut(s) 906, 1062, 1664, 1684
BfuI GTATCC 2 cut(s) 341, 1464
BisI GCNGC 5 cut(s) 154, 438, 704, 759, 1336
BlpI GCTNAGC 1 cut(s) 1668
BlsI GCNGC 5 cut(s) 155, 439, 705, 760, 1337
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 3 cut(s) 275, 803, 986
BmgT120I GGNCC 2 cut(s) 784, 1069
BmrFI CCNGG 3 cut(s) 275, 803, 986
BmrI ACTGGG 2 cut(s) 1300, 1428
BmsI GCATC 7 cut(s) 84, 140, 154, 307, 597, 993, 1602
BmuI ACTGGG 2 cut(s) 1300, 1428
BpmI CTGGAG 1 cut(s) 1675
Bpu1102I GCTNAGC 1 cut(s) 1668
BsaJI CCNNGG 2 cut(s) 801, 932
BsaWI WCCGGW 1 cut(s) 720
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 657, 1456
Bse118I RCCGGY 2 cut(s) 452, 786
Bse1I ACTGG 3 cut(s) 1306, 1423, 1673
Bse3DI GCAATG 3 cut(s) 268, 545, 1504
BseBI CCWGG 3 cut(s) 275, 803, 986
BseDI CCNNGG 2 cut(s) 801, 932
BseGI GGATG 6 cut(s) 155, 169, 667, 1125, 1217, 1593
BseLI CCNNNNNNNGG 3 cut(s) 208, 657, 1456
BseMI GCAATG 3 cut(s) 268, 545, 1504
BseMII CTCAG 7 cut(s) 81, 848, 963, 1126, 1524, 1588, 1682
BseNI ACTGG 3 cut(s) 1306, 1423, 1673
BseRI GAGGAG 2 cut(s) 637, 1026
BseXI GCAGC 5 cut(s) 165, 449, 715, 770, 1347
BseYI CCCAGC 1 cut(s) 1645
BsgI GTGCAG 1 cut(s) 924
BshFI GGCC 5 cut(s) 786, 806, 984, 1071, 1660
BsiHKAI GWGCWC 1 cut(s) 1285
BsiSI CCGG 3 cut(s) 453, 721, 787
BslFI GGGAC 3 cut(s) 981, 1187, 1662
BslI CCNNNNNNNGG 3 cut(s) 208, 657, 1456
BsmAI GTCTC 1 cut(s) 1321
BsmFI GGGAC 3 cut(s) 981, 1187, 1662
BsnI GGCC 5 cut(s) 786, 806, 984, 1071, 1660
Bsp1286I GDGCHC 2 cut(s) 1285, 1425
Bsp1407I TGTACA 1 cut(s) 993
Bsp143I GATC 2 cut(s) 685, 1617
Bsp1720I GCTNAGC 1 cut(s) 1668
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 1 cut(s) 1288
BspANI GGCC 5 cut(s) 786, 806, 984, 1071, 1660
BspCNI CTCAG 7 cut(s) 82, 847, 962, 1125, 1525, 1587, 1681
BspHI TCATGA 1 cut(s) 1077
BsrDI GCAATG 3 cut(s) 268, 545, 1504
BsrFI RCCGGY 2 cut(s) 452, 786
BsrGI TGTACA 1 cut(s) 993
BsrI ACTGG 3 cut(s) 1306, 1423, 1673
BssAI RCCGGY 2 cut(s) 452, 786
BssECI CCNNGG 2 cut(s) 801, 932
BssMI GATC 2 cut(s) 685, 1617
BssT1I CCWWGG 1 cut(s) 932
Bst2UI CCWGG 3 cut(s) 275, 803, 986
Bst4CI ACNGT 5 cut(s) 68, 928, 993, 1324, 1380
Bst6I CTCTTC 1 cut(s) 1143
BstAUI TGTACA 1 cut(s) 993
BstC8I GCNNGC 5 cut(s) 54, 788, 1056, 1425, 1429
BstDEI CTNAG 8 cut(s) 90, 111, 834, 949, 1112, 1533, 1574, 1668
BstDSI CCRYGG 1 cut(s) 932
BstF5I GGATG 6 cut(s) 155, 169, 667, 1125, 1217, 1593
BstHHI GCGC 1 cut(s) 1008
BstKTI GATC 2 cut(s) 688, 1620
BstMAI GTCTC 1 cut(s) 1321
BstMBI GATC 2 cut(s) 685, 1617
BstMWI GCNNNNNNNGC 6 cut(s) 812, 873, 911, 1129, 1224, 1517
BstNI CCWGG 3 cut(s) 275, 803, 986
BstNSI RCATGY 2 cut(s) 862, 1058
BstSCI CCNGG 3 cut(s) 273, 801, 984
BstV1I GCAGC 5 cut(s) 165, 449, 715, 770, 1347
BsuI GTATCC 2 cut(s) 341, 1464
BsuRI GGCC 5 cut(s) 786, 806, 984, 1071, 1660
BtgI CCRYGG 1 cut(s) 932
BtsCI GGATG 6 cut(s) 155, 169, 667, 1125, 1217, 1593
BtsIMutI CAGTG 4 cut(s) 933, 1120, 1235, 1680
Cac8I GCNNGC 5 cut(s) 54, 788, 1056, 1425, 1429
CciI TCATGA 1 cut(s) 1077
CfoI GCGC 1 cut(s) 1008
Cfr10I RCCGGY 2 cut(s) 452, 786
Cfr13I GGNCC 2 cut(s) 784, 1069
Csp6I GTAC 5 cut(s) 12, 443, 924, 994, 1569
CviQI GTAC 5 cut(s) 12, 443, 924, 994, 1569
DdeI CTNAG 8 cut(s) 90, 111, 834, 949, 1112, 1533, 1574, 1668
DpnI GATC 2 cut(s) 687, 1619
DpnII GATC 2 cut(s) 685, 1617
DraI TTTAAA 3 cut(s) 79, 673, 1485
Eam1104I CTCTTC 1 cut(s) 1143
EarI CTCTTC 1 cut(s) 1143
EciI GGCGGA 1 cut(s) 1277
Eco130I CCWWGG 1 cut(s) 932
Eco147I AGGCCT 2 cut(s) 984, 1660
Eco24I GRGCYC 1 cut(s) 1425
Eco32I GATATC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 906, 1601
EcoO109I RGGNCCY 1 cut(s) 1069
EcoRII CCWGG 3 cut(s) 273, 801, 984
EcoRV GATATC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 932
EcoT22I ATGCAT 3 cut(s) 99, 337, 547
EcoT38I GRGCYC 1 cut(s) 1425
ErhI CCWWGG 1 cut(s) 932
FalI AAGNNNNNCTT 4 cut(s) 666, 698, 1604, 1636
FaqI GGGAC 3 cut(s) 981, 1187, 1662
FauNDI CATATG 1 cut(s) 955
Fnu4HI GCNGC 5 cut(s) 154, 438, 704, 759, 1336
FokI GGATG 6 cut(s) 162, 176, 674, 1112, 1224, 1580
FriOI GRGCYC 1 cut(s) 1425
Fsp4HI GCNGC 5 cut(s) 154, 438, 704, 759, 1336
FspBI CTAG 4 cut(s) 906, 1062, 1664, 1684
FspI TGCGCA 1 cut(s) 1007
GlaI GCGC 1 cut(s) 1007
GluI GCNGC 5 cut(s) 154, 438, 704, 759, 1336
GsaI CCCAGC 1 cut(s) 1649
GsuI CTGGAG 1 cut(s) 1675
HaeIII GGCC 5 cut(s) 786, 806, 984, 1071, 1660
HapII CCGG 3 cut(s) 453, 721, 787
HhaI GCGC 1 cut(s) 1008
Hin6I GCGC 1 cut(s) 1006
HinP1I GCGC 1 cut(s) 1006
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 1553
HinfI GANTC 8 cut(s) 251, 824, 830, 969, 1033, 1402, 1529, 1650
HpaII CCGG 3 cut(s) 453, 721, 787
HphI GGTGA 1 cut(s) 1553
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 7 cut(s) 424, 771, 829, 963, 1534, 1551, 1577
Hpy188III TCNNGA 8 cut(s) 147, 683, 1037, 1078, 1318, 1468, 1621, 1654
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 4 cut(s) 365, 429, 958, 974
HpyCH4III ACNGT 5 cut(s) 68, 928, 993, 1324, 1380
HpyF10VI GCNNNNNNNGC 6 cut(s) 812, 873, 911, 1129, 1224, 1517
HpyF3I CTNAG 8 cut(s) 90, 111, 834, 949, 1112, 1533, 1574, 1668
HspAI GCGC 1 cut(s) 1006
KroI GCCGGC 1 cut(s) 786
KroNI GCCGGC 1 cut(s) 788
Kzo9I GATC 2 cut(s) 685, 1617
Lsp1109I GCAGC 5 cut(s) 165, 449, 715, 770, 1347
LweI GCATC 7 cut(s) 84, 140, 154, 307, 597, 993, 1602
MaeI CTAG 4 cut(s) 906, 1062, 1664, 1684
MaeIII GTNAC 6 cut(s) 19, 124, 322, 410, 1018, 1541
MalI GATC 2 cut(s) 687, 1619
MboI GATC 2 cut(s) 685, 1617
MboII GAAGA 5 cut(s) 989, 1130, 1319, 1369, 1594
MhlI GDGCHC 2 cut(s) 1285, 1425
MlyI GAGTC 3 cut(s) 963, 1396, 1644
MmeI TCCRAC 2 cut(s) 597, 820
Mph1103I ATGCAT 3 cut(s) 99, 337, 547
MroNI GCCGGC 1 cut(s) 786
MroXI GAANNNNTTC 2 cut(s) 180, 728
MseI TTAA 6 cut(s) 78, 204, 381, 507, 672, 1484
MslI CAYNNNNRTG 3 cut(s) 195, 573, 779
MspA1I CMGCKG 2 cut(s) 17, 821
MspI CCGG 3 cut(s) 453, 721, 787
MspR9I CCNGG 3 cut(s) 275, 803, 986
MvaI CCWGG 3 cut(s) 275, 803, 986
MwoI GCNNNNNNNGC 6 cut(s) 812, 873, 911, 1129, 1224, 1517
NaeI GCCGGC 1 cut(s) 788
NcoI CCATGG 1 cut(s) 932
NdeI CATATG 1 cut(s) 955
NdeII GATC 2 cut(s) 685, 1617
NgoMIV GCCGGC 1 cut(s) 786
NmuCI GTSAC 2 cut(s) 124, 1541
NsbI TGCGCA 1 cut(s) 1007
NsiI ATGCAT 3 cut(s) 99, 337, 547
NspI RCATGY 2 cut(s) 862, 1058
PaeI GCATGC 1 cut(s) 1058
PagI TCATGA 1 cut(s) 1077
PceI AGGCCT 2 cut(s) 984, 1660
PciI ACATGT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1473
PdiI GCCGGC 1 cut(s) 788
PdmI GAANNNNTTC 2 cut(s) 180, 728
PfeI GAWTC 5 cut(s) 251, 824, 830, 1033, 1529
PkrI GCNGC 5 cut(s) 155, 439, 705, 760, 1337
PleI GAGTC 3 cut(s) 963, 1396, 1644
PpsI GAGTC 3 cut(s) 963, 1396, 1644
PscI ACATGT 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 273, 801, 984
PspFI CCCAGC 1 cut(s) 1645
PspGI CCWGG 3 cut(s) 273, 801, 984
PspPI GGNCC 2 cut(s) 784, 1069
PsrI GAACNNNNNNTAC 2 cut(s) 171, 203
PvuII CAGCTG 2 cut(s) 17, 821
RsaI GTAC 5 cut(s) 13, 444, 925, 995, 1570
RsaNI GTAC 5 cut(s) 12, 443, 924, 994, 1569
RseI CAYNNNNRTG 3 cut(s) 195, 573, 779
SaqAI TTAA 6 cut(s) 78, 204, 381, 507, 672, 1484
SatI GCNGC 5 cut(s) 154, 438, 704, 759, 1336
Sau3AI GATC 2 cut(s) 685, 1617
Sau96I GGNCC 2 cut(s) 784, 1069
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 3 cut(s) 963, 1396, 1644
ScrFI CCNGG 3 cut(s) 275, 803, 986
SduI GDGCHC 2 cut(s) 1285, 1425
SfaNI GCATC 7 cut(s) 84, 140, 154, 307, 597, 993, 1602
SmiMI CAYNNNNRTG 3 cut(s) 195, 573, 779
SphI GCATGC 1 cut(s) 1058
SseBI AGGCCT 2 cut(s) 984, 1660
SsiI CCGC 1 cut(s) 1288
SspI AATATT 2 cut(s) 33, 1089
SspMI CTAG 4 cut(s) 906, 1062, 1664, 1684
StuI AGGCCT 2 cut(s) 984, 1660
StyD4I CCNGG 3 cut(s) 273, 801, 984
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 5 cut(s) 68, 928, 993, 1324, 1380
TaqI TCGA 3 cut(s) 353, 552, 1319
TatI WGTACW 3 cut(s) 442, 923, 993
TfiI GAWTC 5 cut(s) 251, 824, 830, 1033, 1529
Tru1I TTAA 6 cut(s) 78, 204, 381, 507, 672, 1484
Tru9I TTAA 6 cut(s) 78, 204, 381, 507, 672, 1484
TscAI CASTG 4 cut(s) 933, 1120, 1242, 1680
TseFI GTSAC 2 cut(s) 124, 1541
TseI GCWGC 5 cut(s) 153, 437, 703, 758, 1335
Tsp45I GTSAC 2 cut(s) 124, 1541
TspDTI ATGAA 5 cut(s) 179, 384, 482, 990, 1066
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 4 cut(s) 933, 1120, 1242, 1680
XapI RAATTY 3 cut(s) 425, 844, 1478
XceI RCATGY 2 cut(s) 862, 1058
XmnI GAANNNNTTC 2 cut(s) 180, 728
XspI CTAG 4 cut(s) 906, 1062, 1664, 1684
ZrmI AGTACT 1 cut(s) 444
Zsp2I ATGCAT 3 cut(s) 99, 337, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.