Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0017789

Physical Location & Seq

Genomic Coordinates Reverse (-)

47725042 .. 47734973

Gene Structure 9932 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0374500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7038 bp

ATGTGGAACAACGGCCAGATTGTGCCGCCCGGCACTGGTGGCTCGTCGATTCCGCCACCGCCCGCGGCTCAGCCCTCTTATACGGTGTTGCCGCCTCCGGCAGATGCCGAGGCCGTGCTCGAAGAGAAGGCTCGTAAATGGCAGCAACTCAATTCCAAACGGTACAGCGACAAGCGCAAGTTTGGCTTCGTCGAGACGCAGAAAGAGGACATGCCTCCTGAGCATGTCAGGAAGATCATTAGAGACCATGGGGACATGTCCTCGAAGAAATTCCGTCACGATAAACGCGTGTATCTAGGAGCACTTAAATTTGTTCCGCATGCAGTTTACAAGCTTCTTGAGAATATGCCAATGCCCTGGGAGCAGGTCCGTGATGTGAAGGTTCTGTACCATATAACTGGGGCAATTACATTTGTGAATGAGATACCTTGGGTTGTTGAACCTATATATCTGGCTCAGTGGGGTTCAATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGACATTTTAAAAGAATGCGGTTTCCACCTTTTGATGATGAGGAACCTCCATTAGATTATGCTGATAATCTGTTAGATGTTGATCCTCTAGAGCCAATTCAACTGGAGTTGGATGAAGACGAAGATTCTGCTGTTTATACTTGGTTTTATGACCATAAACCTCTAGTCAAGACAAAACTCATTAACGGTCCAAGTTACCGGAAATGGCATCTTTCCCTTCCAATTATGGCAACTCTCCACCGGCTTGCAGGACAGCTACTGTCTGATTTGATAGACCGCAATTATTTCTACTTGTTTGACATGGAGTCTTTCTTTACAGCGAAAGCACTCAATATGTGCATTCCAGGTGGGCCTAAGTTTGAACCATTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATTAACAAGCTGATTATTCGGTCACCTCTCAGAACAGAGTACAGAATTGCGTTCCCTCATCTCTACAACAATAGACCCAGGAAGGTAAAGCTCTGTGTGTATCACACACCCATGGTTATGTACATAAAAACTGAGGATCCTGATCTACCTGCATTTTATTATGATCCCTTGATACACCCAATCCCCAGCACAAACAAGGAGCGACGTGAAAAGAAGATTGATGAGGATGATGACACTTTCATACTGCCAGAAGGGGTAGAACCATTTCTGAGTGATACTCAGCTTTATACTGACACAACAGCAGCTGGAGTTTCCCTCTTGTTTGCACCACGCCCCTTTAACATGAGATCTGGGCGGACTCGCCGTGCAGAAGATATACCTCTTGTGTCAGAGTGGTATAAAGAGCATTGTCCTCCGTCCTATCCAGTGAAAGTGCGGGTCAGCTATCAGAAATTGTTAAAATGTTTTGTGTTGAACGAGTTGCATCATAGACCACCCAAGGCTCAAAAGAAGAAGCACTTGTTTCGATCACTTCAAGCTACTAAGTTCTTCCAAACTACAGAACTTGATTGGGCTGAAGCGGGTCTTCAAGTCTGCAAGCAGGGTTATAACATGCTAAATCTCTTGATCCACAGGAAAAATTTGAATTACCTTCACCTTGATTATAATTTCAATCTGAAGCCTGTGAAAACCCTGACAACTAAAGAGCGTAAAAAGTCCCGGTTTGGTAATGCTTTTCATCTTTGCCGTGAGATCTTGCGGTTGACAAAGCTGGTAGTTGATGCCAACATCCAGTTCCGTTTGGGTAATGTCGATGCCTTTCAATTGGCTGATGGCCTGCAGTACACTTTCTCTCATGTTGGCCAGTTGACTGGTATGTATCGTTACAAATACAGGTTGATGCGTCAGATTAGAATGTGCAAAGATCTGAAGCACTTGATTTACTACCGTTTCAACACGGGACCAGTTGGGAAAGGACCTGGATGTGGATTCTGGGCGCCGATGTGGAGGGTTTGGTTGTTCTTCCTTCGTGGCATAGTTCCTCTTTTGGAACGGTGGTTGGGAAATTTACTTGCACGGCAATTTGAAGGGCGCCATTCAAAAGGAGTGGCAAAAACTGTAACTAAGCAGCGTGTGGAAAGCCATTTTGATTTGGAGCTTCGAGCTGCTGTCATGCATGATGTTCTTGATGCGATGCCTGAGGGAATCAAGCAAAATAAGGCCAGAACTATATTGCAACATCTCAGTGAGGCATGGCGTTGCTGGAAAGCAAATATTCCTTGGAAGGTACCTGGTTTGCCAGTTCCCATTGAGAATATGATTCTTCGTTATGTCAAGTCAAAGGCAGACTGGTGGACAAATGTGGCTCATTACAATCGTGAACGTATAAGAAGAGGTGCTACAGTAGATAAGACAGTTTGTCGGAAGAATCTTGGAAGATTAACACGCCTTTGGCTGAAGGCAGAGCAGGAACGACAACACAATTATCTCAAAGATGGCCCATATGTTACACCAGAGGAGGCAGTTGCAATTTACACGACAACTGTGCATTGGTTGGAGTCAAGAAAGTTCAGCCCCATCCCATTTCCTCCGTTGTCGTACAAGCATGATACCAAGCTACTCATCCTTGCTCTGGAGAGGTTGAAGGAATCATATAGTGTTGCTGTGAGACTGAATCAGCTTCAAAGAGAAGAGCTTGGTCTTATTGAACAAGCCTATGATAACCCGCATGAAGCATTGTCACGAATTAAGCGTCACTTACTCACTCAGCGTGCCTTCAAAGAAGTTGGCATAGAGTTCATGGATTTGTACAGCTATCTCATTCCAGTCTATGAGATTGAACCTCTTGAGAAGATTACAGATGCATATCTCGATCAATATCTATGGTATGAGGGTGACAAACGTCATCTCTTCCCGAACTGGATCAAGCCTGCAGATTCGGAGCCGCCTCCACTCTTGGTTTATAAATGGTGTCAGGGAATAAACAATTTACAGAGTATCTGGGACACGAGTGACGGGCAGTGTGTTGTCATGCTTCAGACCAAGTTTGAGAAGTTCTTTGAAAAGATTGACTTGACAATGCTAAATAGGCTTCTACGTCTAGTTCTTGATCATAATATCGCTGATTATGTCACTGCGAAGAACAATGTGGTATTGTCCTACAAGGATATGAGTCATACAAATTCATATGGTCTTATTCGCGGTCTTCAGTTTGCATCCTTTGTTGTACAGTATTATGGGCTTGTGTTGGATCTATTGCTTCTTGGATTGACTCGAGCCAGTGAGATTGCTGGTCCACCCCAGATGCCAAATGAGTTCATCACTTATTGGGACACAAAGGTCGAGACCCGGCATCCTATCAGGCTGTACTCTCGATACATAGACAGGGTGCACATCTTGTTCCGCTTCACTCATGAGGAAGCTCGGGATCTTATCCAGCGATATCTCACTGAACATCCTGATCCTAATAATGAGAATATGGTGGGATATAACAACAAGAAGTGCTGGCCAAGAGATGCCAGAATGAGGCTCATGAAACATGATGTCAATCTTGGAAGGAGTGTTTTCTGGGATATGAAAAACCGTCTCCCTCGAAGCATCACAACTTTAGAGTGGGAGAACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTCTTCAGCATGTGTGGATTTGAGGTTCGGATACTTCCCAAGATAAGAATGAGCCAAGAGGCATTCAGCAACACAAGAGATGGAGTTTGGAATTTGCAGAATGAACAGACAAAGGAAAGGACTGCAGTTGCTTTCTTACGTGTTGATGATGAACACATGAAAGTATTTGAGAATCGTGTGAGGCAGATCCTTATGTCGTCAGGGTCTACAACATTTACAAAGATTGTCAACAAATGGAATACAGCTCTGATTGGTCTTATGACTTACTTCCGGGAAGCAACTGTACATACGCAAGAACTGTTAGATTTACTAGTCAAGTGCGAAAACAAAATTCAGACCCGTATTAAGATTGGATTAAATTCAAAGATGCCTAGTAGGTTTCCACCTGTCATCTTTTACACACCAAAAGAAATTGGAGGACTTGGAATGTTATCTATGGGTCACATATTGATTCCCCAAAGTGATCTCAGATACAGTCAGCAGACAGATGTTGGTGTGACACACTTTAGGAGTGGGATGAGTCATGAAGAGGACCAGCTGATTCCAAATCTGTACCGTTACATACAACCTTGGGAGAGTGAATTCATTGATTCCCAGCGGGTTTGGGCAGAATATGCTCTGAAAAGGCAGGAGGCTCAAGCACAAAACAGGCGCCTAACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATTCCACGCATAAATACTTTGTTCCAGAAAGATCGGCACACTTTAGCATATGACAAAGGGTGGAGAGTTCGTACTGATTTTAAGCAGTACCAGGTCTTGAAACAAAATCCTTTTTGGTGGACACACCAAAGGCATGACGGAAAACTTTGGAACTTGAACAACTACAGAACTGATGTTATTCAAGCTCTTGGAGGTGTGGAGGGAATCTTGGAGCATACCTTATTTAAAGGAACATACTTCCCTACCTGGGAGGGTCTCTTCTGGGAAAAAGCATCTGGTTTTGAGGAATCGATGAAGTACAAGAAGCTGACTAATGCACAGAGATCTGGGCTCAACCAAATTCCCAATCGTAGGTTCACGCTTTGGTGGTCGCCGACCATTAATCGGGCTAATGTATATGTCGGTTTCCAAGTGCAGTTAGATCTTACTGGGATCTTTATGCATGGGAAAATTCCAACCTTGAAGATATCCTTGATTCAAATATTCCGTGCACATTTGTGGCAAAAGATCCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAGGAGTTAGATGCTTTGGAAATTGAGACCGTGCAGAAGGAAACGATTCATCCCAGGAAGAGTTACAAAATGAACAGTTCTTGTGCTGACATTCTACTCTTTGCTGCCCATAGATGGCCAATGTCAAAACCTAGTCTTGTGGCGGAGCCAAAGGATGTGTTTGATCAGAAAGCAAGCAACAAGTACTGGATAGATGTACAGCTCCGATGGGGAGATTATGATTCCCACGATATTGAACGTTACACCCGAGCCAAGTTTATGGATTATACAACAGACAACATGTCAATCTATCCCTCCCCCACAGGGGTGATGATTGGTCTTGATCTGGCGTATAATTTACATTCTGCTTTTGGTAATTGGTTTCCTGGGTCCAAACCACTGCTTCAGCAGGCAATGAACAAAATAATGAAGTCAAATCCAGCTTTGTACGTCTTGCGGGAGCGGATACGGAAAGGATTGCAGCTTTACTCTTCAGAGCCTACAGAACCTTACTTGTCATCTCAGAACTATGGGGAGATTTTCAGCAATCAAATTATTTGGTTTGTTGATGATACTAATGTGTATCGTGTTACAATCCACAAGACATTTGAAGGAAATCTCACTACAAAACCAATTAATGGTGCGATTTTCATATTCAATCCAAGGACTGGGCAGCTGTTCCTGAAGGTCATCCACACAAGTGTATGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCCAAGTGGAAAACCGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTACCAGTTGAAGAGCAGCCAAAACAAATTATTGTGACTCGAAAGGGAATGCTTGATCCGCTGGAGGTGCACTTGTTGGATTTCCCTAACATAGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTCCAGGCTTGTCTTAAGATTGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAGCCACAGATGGTTCTTTTCAACATCTATGATGATTGGTTGAAGAGTATATCATCATATACTGCATTCTCTCGGCTCATTTTGATTCTTCGTGCACTTCATGTGAACAATGAGAAGGCAAAGATGTTACTGAAACCTGATACAACGGTCATTACTGAGCCACACCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTAAGGGATCTGATTCTCTCAGATTATGCAAAAAAGAACAACGTGAACACATCGGCTCTGACACAATCTGAGATTCGTGATATCATTCTTGGAGCTGAGATTACTCCTCCTTCACAACAAAGACAACAAATTGCAGAGATTGAAAAGCAGCATAAAGAAGCAAGTCAGCTGACAGCAGTTACTACTAGGACCACGAATGTGCATGGTGATGAACTCATTGTCACCACTACTAGTCCTTATGAGCAAGCTGCATTTGGGTCCAAGACTGATTGGCGTGTGAGAGCAATATCGGCTACCAATCTCTACCTTCGTGTCAATCATATATATGTGAATTCAGAGGATGTGAAGGAAACGGGTTACACTTATATCATGCCAAAGAACATATTGAAGAAGTTCATCTGTGTTGCGGATCTGAGGACACAAATTGCTGGTTACTTGTACGGTGTAAGCCCACCTGACAACCCTCAGGTTAAGGAAATTCGCTGCATTGCCATGCCACCGCAGTGGGGAACCCACCAGCAAGTTAATCTTCCAACGGCTCTTCCCGAGCATGATTTCCTCAATGATCTGGAACCTTTGGGATGGATGCACACACAACCAAACGAACTTCCTCAGCTTTCTCCTCAGGATCTAACCTCTCATGCTAAGATCTTGGAAAGCACCAAGCAATGGGATGGGGAGAAGTGCATCATTTTAACCTGCAGTTTCACTCCTGGTTCCTGCTCGTTAACTGCATACAAACTCACTCCATCTGGTTATGAGTGGGGACGGGTCAACAAAGACACAGGAAGCAATCCACACGGTTACCTCCCAACTCATTACGAGAAGGTCCAAATGCTTCTCAGTGATCGGTTCCTTGGTTTCTATATGATTCCTGATAATGGTCCATGGAACTACAACTTCATGGGGGTGAAGCATACACAAGGCATGAAGTATGGTATCAAGCTTGGGACACCGAGGGAGTACTATCATGAGGACCACCGGCCAACCCATTACCTCGAGTTTAGCAACATGGAGGAGGGTGACACAGTGGTGGGAGATCGTGACGATATATTCACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005788 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016592 GO:0022414 GO:0030154 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0060560 GO:0070013 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

2345

Amino Acids

273.96

Weight (kDa)

8.9

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRO8NT	PF08082	68 - 219	4.3e-85	PRO8NT (NUC069), PrP8 N-terminal domain
PROCN	PF08083	405 - 810	1.1e-235	PROCN (NUC071) domain
RRM_4	PF10598	995 - 1085	1.4e-46	RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg	PF10597	1247 - 1382	4.1e-75	U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg	PF10596	1452 - 1610	8.7e-89	U6-snRNA interacting domain of PrP8
PRP8_domainIV	PF12134	1770 - 1999	2.6e-129	PRP8 domain IV core
JAB	PF01398	2110 - 2209	1.1e-11	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT	PF08084	2222 - 2342	2.3e-48	PROCT (NUC072) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017789)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0142581
rosa_laevigata	RLG00000019972
rosa_multiflora	Rmu_co8304105.1_g000001 Rmu_sc0004937.1_g000006
rosa_roxburghii	Rroxscaffold_2G00102470
rosa_rugosa	Rorug02G0374500
rosa_samantha	Rh2AG426100 Rh2AG426200 Rh2BG435800 Rh2CG412700 Rh2DG445600
rosa_wichuraiana	Rw2G034930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1530, 1587, 2886
AasI	GACNNNNNNGTC	2 cut(s)	3260, 5523
Acc36I	ACCTGC	3 cut(s)	355, 1078, 6683
Acc65I	GGTACC	1 cut(s)	2209
AccB1I	GGYRCC	4 cut(s)	1918, 2013, 2209, 4233
AccB7I	CCANNNNNTGG	3 cut(s)	5441, 5471, 6645
AccBSI	CCGCTC	1 cut(s)	5266
AccI	GTMKAC	2 cut(s)	505, 3788
AccII	CGCG	3 cut(s)	65, 288, 3123
AclI	AACGTT	1 cut(s)	5062
AcoI	YGGCCR	6 cut(s)	13, 1783, 3428, 4940, 5538, 6959
AcyI	GRCGYC	3 cut(s)	1919, 2014, 4234
AflII	CTTAAG	1 cut(s)	5720
AflIII	ACRYGT	4 cut(s)	255, 286, 3721, 5103
AhdI	GACNNNNNGTC	3 cut(s)	808, 2340, 5104
AhlI	ACTAGT	2 cut(s)	3892, 6206
AjiI	CACGTC	1 cut(s)	1127
AjuI	GAANNNNNNNTTGG	4 cut(s)	1700, 1732, 4613, 4645
AleI	CACNNNNGTG	6 cut(s)	4771, 5129, 5502, 6173, 6478, 7007
AloI	GAACNNNNNNTCC	2 cut(s)	3591, 3623
Alw21I	GWGCWC	6 cut(s)	120, 304, 3315, 4768, 5655, 5857
Alw26I	GTCTC	7 cut(s)	188, 237, 2584, 3260, 3512, 4535, 4844
Alw44I	GTGCAC	4 cut(s)	3311, 4764, 5651, 5853
AlwNI	CAGNNNCTG	2 cut(s)	763, 1226
Ama87I	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
ApaLI	GTGCAC	4 cut(s)	3311, 4764, 5651, 5853
ArsI	GACNNNNNNTTYG	2 cut(s)	2659, 2691
AseI	ATTAAT	2 cut(s)	4656, 5439
Asp700I	GAANNNNTTC	4 cut(s)	269, 1185, 4869, 6368
Asp718I	GGTACC	1 cut(s)	2209
AspLEI	GCGC	4 cut(s)	177, 1921, 2016, 4236
AsuC2I	CCSGG	4 cut(s)	30, 1642, 3271, 3854
AvaI	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
AxyI	CCTNAGG	3 cut(s)	2121, 6441, 6600
BaeGI	GKGCMC	4 cut(s)	3315, 4768, 5655, 5857
BaeI	ACNNNNGTAYC	8 cut(s)	275, 308, 857, 890, 5367, 5400, 5892, 5925
BalI	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
BamHI	GGATCC	1 cut(s)	1057
BanI	GGYRCC	4 cut(s)	1918, 2013, 2209, 4233
BanII	GRGCYC	1 cut(s)	4608
BarI	GAAGNNNNNNTAC	6 cut(s)	371, 403, 2305, 2337, 3849, 3881
BauI	CACGAG	1 cut(s)	2929
BbsI	GAAGAC	3 cut(s)	627, 1499, 3119
Bbv12I	GWGCWC	6 cut(s)	120, 304, 3315, 4768, 5655, 5857
BbvCI	CCTCAGC	1 cut(s)	6588
BceAI	ACGGC	6 cut(s)	28, 98, 1269, 1653, 2015, 6528
BcgI	CGANNNNNNTGC	6 cut(s)	614, 648, 1427, 1461, 2449, 2483
BciVI	GTATCC	2 cut(s)	3606, 5262
BclI	TGATCA	3 cut(s)	3031, 4987, 5950
BcnI	CCSGG	4 cut(s)	30, 1642, 3271, 3854
BcoDI	GTCTC	7 cut(s)	188, 237, 2584, 3260, 3512, 4535, 4844
BcuI	ACTAGT	2 cut(s)	3892, 6206
BfaI	CTAG	9 cut(s)	296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
BfmI	CTRYAG	9 cut(s)	1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
BfoI	RGCGCY	3 cut(s)	1922, 2017, 4237
BfrI	CTTAAG	1 cut(s)	5720
BfuAI	ACCTGC	3 cut(s)	355, 1078, 6683
BfuI	GTATCC	2 cut(s)	3606, 5262
BglII	AGATCT	6 cut(s)	1268, 1674, 1846, 4598, 4696, 6624
BlpI	GCTNAGC	1 cut(s)	69
BmcAI	AGTACT	2 cut(s)	5009, 6941
BmeRI	GACNNNNNGTC	3 cut(s)	808, 2340, 5104
BmeT110I	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
BmgBI	CACGTC	1 cut(s)	1127
BmrI	ACTGGG	3 cut(s)	408, 4713, 5481
BmuI	ACTGGG	3 cut(s)	408, 4713, 5481
BoxI	GACNNNNGTC	1 cut(s)	2823
BpiI	GAAGAC	3 cut(s)	627, 1499, 3119
BplI	GAGNNNNNCTC	4 cut(s)	1183, 1215, 1221, 1253
BpmI	CTGGAG	4 cut(s)	629, 1248, 2576, 5666
Bpu10I	CCTNAGC	2 cut(s)	219, 6588
Bpu1102I	GCTNAGC	1 cut(s)	69
BpuEI	CTTGAG	3 cut(s)	359, 2789, 4203
BpuMI	CCSGG	4 cut(s)	30, 1642, 3271, 3854
Bsa29I	ATCGAT	1 cut(s)	4565
BsaAI	YACGTR	1 cut(s)	3722
BsaBI	GATNNNNATC	5 cut(s)	585, 1062, 2787, 2799, 3468
BsaHI	GRCGYC	3 cut(s)	1919, 2014, 4234
BsaI	GGTCTC	4 cut(s)	237, 3260, 4535, 4844
BsaWI	WCCGGW	1 cut(s)	702
BsaXI	ACNNNNNCTCC	2 cut(s)	4330, 4360
Bse118I	RCCGGY	2 cut(s)	744, 6957
Bse21I	CCTNAGG	3 cut(s)	2121, 6441, 6600
Bse3DI	GCAATG	3 cut(s)	5223, 6462, 6650
Bse8I	GATNNNNATC	5 cut(s)	585, 1062, 2787, 2799, 3468
BseCI	ATCGAT	1 cut(s)	4565
BseJI	GATNNNNATC	5 cut(s)	585, 1062, 2787, 2799, 3468
BseMI	GCAATG	3 cut(s)	5223, 6462, 6650
BseRI	GAGGAG	4 cut(s)	2453, 6072, 6588, 7007
BseSI	GKGCMC	4 cut(s)	3315, 4768, 5655, 5857
BseYI	CCCAGC	2 cut(s)	1106, 4176
BsgI	GTGCAG	4 cut(s)	1308, 4709, 4877, 5553
Bsh1236I	CGCG	3 cut(s)	65, 288, 3123
BshNI	GGYRCC	4 cut(s)	1918, 2013, 2209, 4233
BshVI	ATCGAT	1 cut(s)	4565
BsiHKAI	GWGCWC	6 cut(s)	120, 304, 3315, 4768, 5655, 5857
BsiHKCI	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
BsiSI	CCGG	8 cut(s)	30, 98, 703, 745, 1642, 3271, 3853, 6958
BslFI	GGGAC	7 cut(s)	266, 1624, 1896, 2939, 3266, 6756, 6940
BsmAI	GTCTC	7 cut(s)	188, 237, 2584, 3260, 3512, 4535, 4844
BsmBI	CGTCTC	2 cut(s)	188, 3512
BsmFI	GGGAC	7 cut(s)	266, 1624, 1896, 2939, 3266, 6756, 6940
BsmI	GAATGC	5 cut(s)	525, 843, 3644, 5637, 5825
Bso31I	GGTCTC	4 cut(s)	237, 3260, 4535, 4844
BsoBI	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
Bsp1286I	GDGCHC	7 cut(s)	120, 304, 3315, 4608, 4768, 5655, 5857
Bsp1407I	TGTACA	5 cut(s)	1041, 2730, 3148, 3865, 5020
Bsp1720I	GCTNAGC	1 cut(s)	69
Bsp19I	CCATGG	3 cut(s)	247, 1032, 6863
BspDI	ATCGAT	1 cut(s)	4565
BspFNI	CGCG	3 cut(s)	65, 288, 3123
BspHI	TCATGA	4 cut(s)	3334, 3453, 4105, 6946
BspMAI	CTGCAG	5 cut(s)	1764, 2857, 3709, 5700, 6680
BspMI	ACCTGC	3 cut(s)	355, 1078, 6683
BspQI	GCTCTTC	4 cut(s)	2607, 3590, 5589, 6522
BspT107I	GGYRCC	4 cut(s)	1918, 2013, 2209, 4233
BspTI	CTTAAG	1 cut(s)	5720
BspTNI	GGTCTC	4 cut(s)	237, 3260, 4535, 4844
BsrBI	CCGCTC	1 cut(s)	5266
BsrDI	GCAATG	3 cut(s)	5223, 6462, 6650
BsrFI	RCCGGY	2 cut(s)	744, 6957
BsrGI	TGTACA	5 cut(s)	1041, 2730, 3148, 3865, 5020
BssAI	RCCGGY	2 cut(s)	744, 6957
BssNI	GRCGYC	3 cut(s)	1919, 2014, 4234
BssSI	CACGAG	1 cut(s)	2929
BssT1I	CCWWGG	9 cut(s)	247, 428, 1032, 1419, 2201, 4151, 5465, 6832, 6863
Bst2BI	CACGAG	1 cut(s)	2929
BstACI	GRCGYC	3 cut(s)	1919, 2014, 4234
BstAFI	CTTAAG	1 cut(s)	5720
BstAPI	GCANNNNNTGC	1 cut(s)	4196
BstAUI	TGTACA	5 cut(s)	1041, 2730, 3148, 3865, 5020
BstBAI	YACGTR	1 cut(s)	3722
BstDSI	CCRYGG	4 cut(s)	63, 247, 1032, 6863
BstEII	GGTNACC	2 cut(s)	942, 6779
BstFNI	CGCG	3 cut(s)	65, 288, 3123
BstH2I	RGCGCY	3 cut(s)	1922, 2017, 4237
BstHHI	GCGC	4 cut(s)	177, 1921, 2016, 4236
BstMAI	GTCTC	7 cut(s)	188, 237, 2584, 3260, 3512, 4535, 4844
BstNSI	RCATGY	7 cut(s)	214, 227, 259, 323, 1537, 3595, 5107
BstPAI	GACNNNNGTC	1 cut(s)	2823
BstPI	GGTNACC	2 cut(s)	942, 6779
BstSFI	CTRYAG	9 cut(s)	1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
BstSLI	GKGCMC	4 cut(s)	3315, 4768, 5655, 5857
BstUI	CGCG	3 cut(s)	65, 288, 3123
BstV2I	GAAGAC	3 cut(s)	627, 1499, 3119
BstXI	CCANNNNNNTGG	5 cut(s)	357, 398, 1793, 5083, 6480
Bsu15I	ATCGAT	1 cut(s)	4565
Bsu36I	CCTNAGG	3 cut(s)	2121, 6441, 6600
BsuI	GTATCC	2 cut(s)	3606, 5262
BsuTUI	ATCGAT	1 cut(s)	4565
BtgI	CCRYGG	4 cut(s)	63, 247, 1032, 6863
BtgZI	GCGATG	1 cut(s)	2129
BtrI	CACGTC	1 cut(s)	1127
BtsI	GCAGTG	4 cut(s)	2948, 3054, 5201, 6485
BveI	ACCTGC	3 cut(s)	355, 1078, 6683
CaiI	CAGNNNCTG	2 cut(s)	763, 1226
CciI	TCATGA	4 cut(s)	3334, 3453, 4105, 6946
CfoI	GCGC	4 cut(s)	177, 1921, 2016, 4236
Cfr10I	RCCGGY	2 cut(s)	744, 6957
Cfr42I	CCGCGG	1 cut(s)	66
ClaI	ATCGAT	1 cut(s)	4565
CseI	GACGC	4 cut(s)	205, 1814, 2663, 5513
CsiI	ACCWGGT	2 cut(s)	2212, 4365
CspCI	CAANNNNNGTGG	2 cut(s)	2010, 2045
DinI	GGCGCC	3 cut(s)	1920, 2015, 4235
DraI	TTTAAA	2 cut(s)	514, 4501
DrdI	GACNNNNNNGTC	2 cut(s)	3260, 5523
DriI	GACNNNNNGTC	3 cut(s)	808, 2340, 5104
DseDI	GACNNNNNNGTC	2 cut(s)	3260, 5523
EaeI	YGGCCR	6 cut(s)	13, 1783, 3428, 4940, 5538, 6959
Eam1105I	GACNNNNNGTC	3 cut(s)	808, 2340, 5104
EciI	GGCGGA	3 cut(s)	42, 1291, 4982
Eco130I	CCWWGG	9 cut(s)	247, 428, 1032, 1419, 2201, 4151, 5465, 6832, 6863
Eco24I	GRGCYC	1 cut(s)	4608
Eco31I	GGTCTC	4 cut(s)	237, 3260, 4535, 4844
Eco32I	GATATC	3 cut(s)	3365, 4743, 6058
Eco81I	CCTNAGG	3 cut(s)	2121, 6441, 6600
Eco88I	CYCGRG	5 cut(s)	3195, 3345, 5070, 6521, 6974
Eco91I	GGTNACC	2 cut(s)	942, 6779
EcoO109I	RGGNCCY	1 cut(s)	1898
EcoO65I	GGTNACC	2 cut(s)	942, 6779
EcoRI	GAATTC	3 cut(s)	4163, 4275, 6307
EcoRV	GATATC	3 cut(s)	3365, 4743, 6058
EcoT14I	CCWWGG	9 cut(s)	247, 428, 1032, 1419, 2201, 4151, 5465, 6832, 6863
EcoT22I	ATGCAT	3 cut(s)	2100, 2788, 4719
EcoT38I	GRGCYC	1 cut(s)	4608
EgeI	GGCGCC	3 cut(s)	1920, 2015, 4235
EheI	GGCGCC	3 cut(s)	1920, 2015, 4235
ErhI	CCWWGG	9 cut(s)	247, 428, 1032, 1419, 2201, 4151, 5465, 6832, 6863
Esp3I	CGTCTC	2 cut(s)	188, 3512
FaqI	GGGAC	7 cut(s)	266, 1624, 1896, 2939, 3266, 6756, 6940
FauI	CCCGC	6 cut(s)	70, 1350, 1495, 2655, 4173, 5253
FauNDI	CATATG	3 cut(s)	2425, 3109, 4324
FbaI	TGATCA	3 cut(s)	3031, 4987, 5950
FblI	GTMKAC	2 cut(s)	505, 3788
FriOI	GRGCYC	1 cut(s)	4608
FspBI	CTAG	9 cut(s)	296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
GlaI	GCGC	4 cut(s)	176, 1920, 2015, 4235
GsaI	CCCAGC	2 cut(s)	1110, 4180
GsuI	CTGGAG	4 cut(s)	629, 1248, 2576, 5666
HaeII	RGCGCY	3 cut(s)	1922, 2017, 4237
HapII	CCGG	8 cut(s)	30, 98, 703, 745, 1642, 3271, 3853, 6958
HgaI	GACGC	4 cut(s)	205, 1814, 2663, 5513
HhaI	GCGC	4 cut(s)	177, 1921, 2016, 4236
Hin1I	GRCGYC	3 cut(s)	1919, 2014, 4234
Hin6I	GCGC	4 cut(s)	175, 1919, 2014, 4234
HinP1I	GCGC	4 cut(s)	175, 1919, 2014, 4234
HincII	GTYRAC	6 cut(s)	506, 1686, 1791, 3811, 6705, 6751
HindII	GTYRAC	6 cut(s)	506, 1686, 1791, 3811, 6705, 6751
HindIII	AAGCTT	2 cut(s)	332, 6920
HpaI	GTTAAC	1 cut(s)	6705
HpaII	CCGG	8 cut(s)	30, 98, 703, 745, 1642, 3271, 3853, 6958
Hpy99I	CGWCG	4 cut(s)	49, 194, 507, 1128
HpyCH4IV	ACGT	8 cut(s)	1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
HpySE526I	ACGT	8 cut(s)	1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
Hsp92I	GRCGYC	3 cut(s)	1919, 2014, 4234
HspAI	GCGC	4 cut(s)	175, 1919, 2014, 4234
KasI	GGCGCC	3 cut(s)	1918, 2013, 4233
KpnI	GGTACC	1 cut(s)	2213
Ksp22I	TGATCA	3 cut(s)	3031, 4987, 5950
KspAI	GTTAAC	1 cut(s)	6705
KspI	CCGCGG	1 cut(s)	66
LguI	GCTCTTC	4 cut(s)	2607, 3590, 5589, 6522
MabI	ACCWGGT	2 cut(s)	2212, 4365
MaeI	CTAG	9 cut(s)	296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
MaeII	ACGT	8 cut(s)	1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
MbiI	CCGCTC	1 cut(s)	5266
MfeI	CAATTG	1 cut(s)	1745
MhlI	GDGCHC	7 cut(s)	120, 304, 3315, 4608, 4768, 5655, 5857
MlsI	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
MluI	ACGCGT	1 cut(s)	286
MluNI	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
Mly113I	GGCGCC	3 cut(s)	1919, 2014, 4234
MlyI	GAGTC	7 cut(s)	818, 1273, 2489, 3103, 3187, 4111, 5614
MmeI	TCCRAC	7 cut(s)	594, 2324, 2457, 3150, 4754, 5640, 6533
Mox20I	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
Mph1103I	ATGCAT	3 cut(s)	2100, 2788, 4719
MroXI	GAANNNNTTC	4 cut(s)	269, 1185, 4869, 6368
MscI	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
Msp20I	TGGCCA	4 cut(s)	1785, 3430, 4942, 5540
MspA1I	CMGCKG	7 cut(s)	65, 1226, 4120, 4180, 5479, 5644, 6145
MspCI	CTTAAG	1 cut(s)	5720
MspI	CCGG	8 cut(s)	30, 98, 703, 745, 1642, 3271, 3853, 6958
MunI	CAATTG	1 cut(s)	1745
Mva1269I	GAATGC	5 cut(s)	525, 843, 3644, 5637, 5825
MvnI	CGCG	3 cut(s)	65, 288, 3123
NarI	GGCGCC	3 cut(s)	1919, 2014, 4234
NciI	CCSGG	4 cut(s)	30, 1642, 3271, 3854
NcoI	CCATGG	3 cut(s)	247, 1032, 6863
NdeI	CATATG	3 cut(s)	2425, 3109, 4324
NmeAIII	GCCGAG	2 cut(s)	133, 5812
NsiI	ATGCAT	3 cut(s)	2100, 2788, 4719
NspI	RCATGY	7 cut(s)	214, 227, 259, 323, 1537, 3595, 5107
OliI	CACNNNNGTG	6 cut(s)	4771, 5129, 5502, 6173, 6478, 7007
PaeI	GCATGC	1 cut(s)	323
PaeR7I	CTCGAG	2 cut(s)	3195, 6974
PagI	TCATGA	4 cut(s)	3334, 3453, 4105, 6946
PasI	CCCWGGG	1 cut(s)	357
PciI	ACATGT	2 cut(s)	255, 5103
PciSI	GCTCTTC	4 cut(s)	2607, 3590, 5589, 6522
PcsI	WCGNNNNNNNCGW	3 cut(s)	285, 2671, 5068
PctI	GAATGC	5 cut(s)	525, 843, 3644, 5637, 5825
PdmI	GAANNNNTTC	4 cut(s)	269, 1185, 4869, 6368
PflMI	CCANNNNNTGG	3 cut(s)	5441, 5471, 6645
PfoI	TCCNGGA	1 cut(s)	3852
PleI	GAGTC	7 cut(s)	817, 1273, 2488, 3102, 3187, 4110, 5614
PluTI	GGCGCC	3 cut(s)	1922, 2017, 4237
PpsI	GAGTC	7 cut(s)	817, 1273, 2488, 3102, 3187, 4110, 5614
Ppu21I	YACGTR	1 cut(s)	3722
PpuMI	RGGWCCY	1 cut(s)	1898
PscI	ACATGT	2 cut(s)	255, 5103
PshAI	GACNNNNGTC	1 cut(s)	2823
PshBI	ATTAAT	2 cut(s)	4656, 5439
PsiI	TTATAA	3 cut(s)	1530, 1587, 2886
Psp1406I	AACGTT	1 cut(s)	5062
Psp5II	RGGWCCY	1 cut(s)	1898
PspEI	GGTNACC	2 cut(s)	942, 6779
PspFI	CCCAGC	2 cut(s)	1106, 4176
PspPPI	RGGWCCY	1 cut(s)	1898
PspXI	VCTCGAGB	2 cut(s)	3195, 6974
PstI	CTGCAG	5 cut(s)	1764, 2857, 3709, 5700, 6680
PstNI	CAGNNNCTG	2 cut(s)	763, 1226
PvuII	CAGCTG	4 cut(s)	1226, 4120, 5479, 6145
SacII	CCGCGG	1 cut(s)	66
SalI	GTCGAC	1 cut(s)	504
SapI	GCTCTTC	4 cut(s)	2607, 3590, 5589, 6522
ScaI	AGTACT	2 cut(s)	5009, 6941
SchI	GAGTC	7 cut(s)	818, 1273, 2489, 3103, 3187, 4111, 5614
SduI	GDGCHC	7 cut(s)	120, 304, 3315, 4608, 4768, 5655, 5857
SexAI	ACCWGGT	2 cut(s)	2212, 4365
SfcI	CTRYAG	9 cut(s)	1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
SfoI	GGCGCC	3 cut(s)	1920, 2015, 4235
Sfr274I	CTCGAG	2 cut(s)	3195, 6974
Sfr303I	CCGCGG	1 cut(s)	66
SgrBI	CCGCGG	1 cut(s)	66
SlaI	CTCGAG	2 cut(s)	3195, 6974
SmlI	CTYRAG	6 cut(s)	338, 2768, 3195, 4218, 5720, 6974
SmoI	CTYRAG	6 cut(s)	338, 2768, 3195, 4218, 5720, 6974
SpeI	ACTAGT	2 cut(s)	3892, 6206
SphI	GCATGC	1 cut(s)	323
SspDI	GGCGCC	3 cut(s)	1918, 2013, 4233
SspI	AATATT	2 cut(s)	2197, 4758
SspMI	CTAG	9 cut(s)	296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
StyI	CCWWGG	9 cut(s)	247, 428, 1032, 1419, 2201, 4151, 5465, 6832, 6863
TaiI	ACGT	8 cut(s)	1129, 2308, 2827, 3022, 3724, 5065, 5256, 6021
TaqII	GACCGA	1 cut(s)	930
TauI	GCSGC	4 cut(s)	28, 68, 94, 2869
TspGWI	ACGGA	8 cut(s)	263, 359, 1326, 1709, 2502, 4428, 4751, 5287
Van91I	CCANNNNNTGG	3 cut(s)	5441, 5471, 6645
Vha464I	CTTAAG	1 cut(s)	5720
VneI	GTGCAC	4 cut(s)	3311, 4764, 5651, 5853
VspI	ATTAAT	2 cut(s)	4656, 5439
XbaI	TCTAGA	1 cut(s)	592
XceI	RCATGY	7 cut(s)	214, 227, 259, 323, 1537, 3595, 5107
XcmI	CCANNNNNNNNNTGG	1 cut(s)	5928
XhoI	CTCGAG	2 cut(s)	3195, 6974
XmiI	GTMKAC	2 cut(s)	505, 3788
XmnI	GAANNNNTTC	4 cut(s)	269, 1185, 4869, 6368
XspI	CTAG	9 cut(s)	296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
ZrmI	AGTACT	2 cut(s)	5009, 6941
Zsp2I	ATGCAT	3 cut(s)	2100, 2788, 4719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.