Rorug02G0393800

von Willebrand factor type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004111
Physical Location & Seq
Forward (+)
50439585 .. 50442210
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0393800.1

Sequence Viewer

Length: 915 bp
ATGGCAGCCCTAGTGTACCAGATGATGTCGTCCACAGCACTGGTCTCACTGGGACTCTACCACATGATCGCCACCACCCGCAACCACCTGAGATTCCCACAGTCCTATTTCGCTAGACCCTACCACCCTTTCCCTCTCTCCACCTCCTCATCATCCCACAATCACCGCCTCAAGTTCCTCCAGCTCTACCTCGTCATTGCATTCCTCGCCGTCGCCATAGTCCACCGAACAGTCGCCTCCTTAGACGCCGATCCACTCCTCAAGGGTCGCACGCCGGTCCACTACTTCACCTCCCTCCAGTCCGCCGCCTCCCTCTTCCTCTTTCTACTCCTCACACTGGCGCTCCTTCTCTCCGAGTGGATCCCATCTTTGCTGCCCCTCCCCACCGACCTCATCTTCGGCCTCGCCGCCGCCCTCTTCTACTTGCAGTCATTTGTCTCTTGGGGCGCCGCAGCCGTCCAGACATCCGACCTTCAGGCCAAATGTGACTCCATCTCAGGAAGGATATCCGCCCAAGCCTCAATCCTCTGCCTCGTCTTAGCTTGCCAGCCAAGACTATTCTTGGCAGACGTAGGCTTGGGCGCTTCCATGTCTCTGCAGGGCCTGTGGGTCCTGCAGACGGGCCTCTCCCTCTCCGTGGATGCCTTCATCCCCGAGGGCTGCCACAAGCTTCTTGATGTGGTCAATGGAGTCGAGGGTTCAACCAAGTGTGATTTGGACGAGTCCAAGTTCAGGGCTGTGGCCATTCTGGATCTAGTGTTCTTGGTGCACGTAATGTTCGTGTTGCTGATTGTGATGGTCACCTATGCAATGACTGCCAAGTCGGTTGGTATTCGGAGAATGGGTTCTTACGAGGCCTTGCCTAATGACAACAGCCACATTCAGATGAAATCCTTGAGTGGCACCCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_3

Protein Analysis

304

Amino Acids

33.14

Weight (kDa)

8.27

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 130 - 270 4e-30 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 820
AccB1I GGYRCC 2 cut(s) 446, 902
AciI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
AclWI GGATC 4 cut(s) 245, 355, 368, 759
AcoI YGGCCR 1 cut(s) 741
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 2 cut(s) 246, 447
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
AgsI TTSAA 1 cut(s) 702
AjnI CCWGG 1 cut(s) 906
AloI GAACNNNNNNTCC 4 cut(s) 743, 775, 829, 861
AluBI AGCT 3 cut(s) 184, 542, 670
AluI AGCT 3 cut(s) 184, 542, 670
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 3 cut(s) 49, 442, 597
Alw44I GTGCAC 1 cut(s) 767
AlwI GGATC 4 cut(s) 245, 355, 368, 759
AlwNI CAGNNNCTG 1 cut(s) 604
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 6 cut(s) 400, 477, 601, 622, 741, 855
ApaLI GTGCAC 1 cut(s) 767
ApeKI GCWGC 4 cut(s) 5, 373, 452, 660
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
Asp700I GAANNNNTTC 1 cut(s) 844
AspLEI GCGC 3 cut(s) 343, 449, 584
AspS9I GGNCC 4 cut(s) 277, 601, 610, 622
AsuHPI GGTGA 3 cut(s) 155, 280, 793
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 2 cut(s) 277, 610
BaeGI GKGCMC 1 cut(s) 771
BalI TGGCCA 1 cut(s) 743
BamHI GGATCC 1 cut(s) 360
BanI GGYRCC 2 cut(s) 446, 902
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 4 cut(s) 17, 360, 464, 647
BccI CCATC 3 cut(s) 373, 500, 790
BceAI ACGGC 2 cut(s) 194, 440
BciT130I CCWGG 1 cut(s) 908
BcoDI GTCTC 3 cut(s) 49, 442, 597
BfaI CTAG 3 cut(s) 11, 114, 755
BfmI CTRYAG 2 cut(s) 596, 614
BfoI RGCGCY 3 cut(s) 344, 450, 585
BisI GCNGC 8 cut(s) 6, 306, 374, 408, 411, 450, 453, 661
BlsI GCNGC 8 cut(s) 7, 307, 375, 409, 412, 451, 454, 662
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 2 cut(s) 277, 610
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 4 cut(s) 277, 601, 610, 622
BmiI GGNNCC 4 cut(s) 362, 448, 611, 904
BmrFI CCNGG 1 cut(s) 908
BmrI ACTGGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 631
BmuI ACTGGG 1 cut(s) 59
BpmI CTGGAG 2 cut(s) 164, 281
BpuEI CTTGAG 2 cut(s) 155, 245
BsaAI YACGTR 1 cut(s) 772
BsaHI GRCGYC 2 cut(s) 246, 447
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 636, 654, 906
BsaXI ACNNNNNCTCC 6 cut(s) 275, 305, 327, 357, 829, 859
Bsc4I CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 45, 54, 298, 342
Bse3DI GCAATG 2 cut(s) 195, 816
BseBI CCWGG 1 cut(s) 908
BseDI CCNNGG 3 cut(s) 636, 654, 906
BseGI GGATG 4 cut(s) 152, 464, 646, 648
BseLI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
BseMI GCAATG 2 cut(s) 195, 816
BseMII CTCAG 2 cut(s) 80, 510
BseNI ACTGG 4 cut(s) 45, 54, 298, 342
BseRI GAGGAG 3 cut(s) 136, 248, 320
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 4 cut(s) 17, 360, 464, 647
BshFI GGCC 6 cut(s) 402, 479, 603, 624, 743, 857
BshNI GGYRCC 2 cut(s) 446, 902
BsiHKAI GWGCWC 1 cut(s) 771
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
BsmAI GTCTC 3 cut(s) 49, 442, 597
BsmFI GGGAC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 6 cut(s) 402, 479, 603, 624, 743, 857
Bso31I GGTCTC 1 cut(s) 49
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 771
Bsp143I GATC 4 cut(s) 66, 250, 360, 751
BspACI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
BspANI GGCC 6 cut(s) 402, 479, 603, 624, 743, 857
BspCNI CTCAG 2 cut(s) 81, 509
BspLI GGNNCC 4 cut(s) 362, 448, 611, 904
BspMAI CTGCAG 2 cut(s) 600, 618
BspPI GGATC 4 cut(s) 245, 355, 368, 759
BspT107I GGYRCC 2 cut(s) 446, 902
BspTNI GGTCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 195, 816
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 4 cut(s) 45, 54, 298, 342
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 3 cut(s) 636, 654, 906
BssMI GATC 4 cut(s) 66, 250, 360, 751
BssNI GRCGYC 2 cut(s) 246, 447
Bst2UI CCWGG 1 cut(s) 908
Bst4CI ACNGT 2 cut(s) 102, 232
Bst6I CTCTTC 2 cut(s) 320, 422
BstACI GRCGYC 2 cut(s) 246, 447
BstAPI GCANNNNNTGC 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 272, 544, 548
BstDEI CTNAG 5 cut(s) 89, 241, 496, 538, 912
BstDSI CCRYGG 1 cut(s) 636
BstEII GGTNACC 1 cut(s) 799
BstF5I GGATG 4 cut(s) 152, 464, 646, 648
BstH2I RGCGCY 3 cut(s) 344, 450, 585
BstHHI GCGC 3 cut(s) 343, 449, 584
BstKTI GATC 4 cut(s) 69, 253, 363, 754
BstMAI GTCTC 3 cut(s) 49, 442, 597
BstMBI GATC 4 cut(s) 66, 250, 360, 751
BstMWI GCNNNNNNNGC 2 cut(s) 206, 815
BstNI CCWGG 1 cut(s) 908
BstPI GGTNACC 1 cut(s) 799
BstSCI CCNGG 1 cut(s) 906
BstSFI CTRYAG 2 cut(s) 596, 614
BstSLI GKGCMC 1 cut(s) 771
BstV1I GCAGC 4 cut(s) 17, 360, 464, 647
BstX2I RGATCY 2 cut(s) 360, 751
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 2 cut(s) 360, 751
BsuRI GGCC 6 cut(s) 402, 479, 603, 624, 743, 857
BtgI CCRYGG 1 cut(s) 636
BtsCI GGATG 4 cut(s) 152, 464, 646, 648
BtsIMutI CAGTG 3 cut(s) 38, 47, 335
Cac8I GCNNGC 3 cut(s) 272, 544, 548
CaiI CAGNNNCTG 1 cut(s) 604
CfoI GCGC 3 cut(s) 343, 449, 584
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 4 cut(s) 277, 601, 610, 622
CseI GACGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 16
CviAII CATG 2 cut(s) 64, 589
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 5 cut(s) 89, 241, 496, 538, 912
DinI GGCGCC 1 cut(s) 448
DpnI GATC 4 cut(s) 68, 252, 362, 753
DpnII GATC 4 cut(s) 66, 250, 360, 751
DrdI GACNNNNNNGTC 1 cut(s) 820
DseDI GACNNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 2 cut(s) 320, 422
EarI CTCTTC 2 cut(s) 320, 422
EciI GGCGGA 2 cut(s) 292, 499
Eco147I AGGCCT 1 cut(s) 857
Eco31I GGTCTC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 277, 610
Eco57I CTGAAG 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 799
EcoO109I RGGNCCY 2 cut(s) 601, 610
EcoO65I GGTNACC 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 906
EcoRV GATATC 1 cut(s) 507
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
FaeI CATG 2 cut(s) 67, 592
FaiI YATR 4 cut(s) 65, 218, 590, 807
FaqI GGGAC 1 cut(s) 66
FatI CATG 2 cut(s) 63, 588
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 8 cut(s) 6, 306, 374, 408, 411, 450, 453, 661
FokI GGATG 4 cut(s) 139, 451, 635, 653
Fsp4HI GCNGC 8 cut(s) 6, 306, 374, 408, 411, 450, 453, 661
FspBI CTAG 3 cut(s) 11, 114, 755
GlaI GCGC 3 cut(s) 342, 448, 583
GluI GCNGC 8 cut(s) 6, 306, 374, 408, 411, 450, 453, 661
GsuI CTGGAG 2 cut(s) 164, 281
HaeII RGCGCY 3 cut(s) 344, 450, 585
HaeIII GGCC 6 cut(s) 402, 479, 603, 624, 743, 857
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 254
HhaI GCGC 3 cut(s) 343, 449, 584
Hin1I GRCGYC 2 cut(s) 246, 447
Hin1II CATG 2 cut(s) 67, 592
Hin6I GCGC 3 cut(s) 341, 447, 582
HinP1I GCGC 3 cut(s) 341, 447, 582
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 54, 93, 488, 690, 722
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 155, 280, 793
Hpy166II GTNNAC 5 cut(s) 16, 33, 223, 280, 769
Hpy188I TCNGA 4 cut(s) 355, 469, 837, 885
Hpy188III TCNNGA 4 cut(s) 460, 498, 674, 749
Hpy8I GTNNAC 5 cut(s) 16, 33, 223, 280, 769
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 356, 482, 495, 655
HpyCH4III ACNGT 2 cut(s) 102, 232
HpyCH4IV ACGT 2 cut(s) 570, 771
HpyCH4V TGCA 6 cut(s) 200, 427, 598, 616, 769, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 815
HpyF3I CTNAG 5 cut(s) 89, 241, 496, 538, 912
HpySE526I ACGT 2 cut(s) 570, 771
Hsp92I GRCGYC 2 cut(s) 246, 447
Hsp92II CATG 2 cut(s) 67, 592
HspAI GCGC 3 cut(s) 341, 447, 582
KasI GGCGCC 1 cut(s) 446
Kzo9I GATC 4 cut(s) 66, 250, 360, 751
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 4 cut(s) 17, 360, 464, 647
LweI GCATC 1 cut(s) 631
MaeI CTAG 3 cut(s) 11, 114, 755
MaeII ACGT 2 cut(s) 570, 771
MaeIII GTNAC 2 cut(s) 485, 799
MalI GATC 4 cut(s) 68, 252, 362, 753
MboI GATC 4 cut(s) 66, 250, 360, 751
MboII GAAGA 3 cut(s) 307, 388, 409
MflI RGATCY 2 cut(s) 360, 751
MhlI GDGCHC 1 cut(s) 771
MlsI TGGCCA 1 cut(s) 743
MluNI TGGCCA 1 cut(s) 743
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 4 cut(s) 48, 482, 699, 731
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 743
MroXI GAANNNNTTC 1 cut(s) 844
MscI TGGCCA 1 cut(s) 743
MslI CAYNNNNRTG 1 cut(s) 884
Msp20I TGGCCA 1 cut(s) 743
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 908
Mva1269I GAATGC 1 cut(s) 200
MvaI CCWGG 1 cut(s) 908
MwoI GCNNNNNNNGC 2 cut(s) 206, 815
NarI GGCGCC 1 cut(s) 447
NdeII GATC 4 cut(s) 66, 250, 360, 751
NlaIII CATG 2 cut(s) 67, 592
NlaIV GGNNCC 4 cut(s) 362, 448, 611, 904
NmuCI GTSAC 2 cut(s) 485, 799
PceI AGGCCT 1 cut(s) 857
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PctI GAATGC 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 1 cut(s) 93
PkrI GCNGC 8 cut(s) 7, 307, 375, 409, 412, 451, 454, 662
PleI GAGTC 4 cut(s) 48, 482, 698, 730
PluTI GGCGCC 1 cut(s) 450
PpsI GAGTC 4 cut(s) 48, 482, 698, 730
Ppu21I YACGTR 1 cut(s) 772
PpuMI RGGWCCY 1 cut(s) 610
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 906
PspEI GGTNACC 1 cut(s) 799
PspGI CCWGG 1 cut(s) 906
PspN4I GGNNCC 4 cut(s) 362, 448, 611, 904
PspPI GGNCC 4 cut(s) 277, 601, 610, 622
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 2 cut(s) 600, 618
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 2 cut(s) 360, 751
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 884
SatI GCNGC 8 cut(s) 6, 306, 374, 408, 411, 450, 453, 661
Sau3AI GATC 4 cut(s) 66, 250, 360, 751
Sau96I GGNCC 4 cut(s) 277, 601, 610, 622
SchI GAGTC 4 cut(s) 48, 482, 699, 731
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 2 cut(s) 596, 614
SfoI GGCGCC 1 cut(s) 448
SinI GGWCC 2 cut(s) 277, 610
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 3 cut(s) 170, 260, 895
SmoI CTYRAG 3 cut(s) 170, 260, 895
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
SspDI GGCGCC 1 cut(s) 446
SspMI CTAG 3 cut(s) 11, 114, 755
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 1 cut(s) 906
TaaI ACNGT 2 cut(s) 102, 232
TaiI ACGT 2 cut(s) 573, 774
TaqI TCGA 1 cut(s) 693
TauI GCSGC 4 cut(s) 308, 410, 413, 452
TfiI GAWTC 1 cut(s) 93
TscAI CASTG 3 cut(s) 45, 54, 342
TseFI GTSAC 2 cut(s) 485, 799
TseI GCWGC 4 cut(s) 5, 373, 452, 660
Tsp45I GTSAC 2 cut(s) 485, 799
TspDTI ATGAA 2 cut(s) 637, 902
TspGWI ACGGA 1 cut(s) 625
TspRI CASTG 3 cut(s) 45, 54, 342
VneI GTGCAC 1 cut(s) 767
VpaK11BI GGWCC 2 cut(s) 277, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 3 cut(s) 11, 114, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.