Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0008225

Physical Location & Seq

Genomic Coordinates Reverse (-)

54838508 .. 54842039

Gene Structure 3532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0427500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGCCACCACCAATTCCATATCAACCACCAAATCTCAAGGCCTTGTGGGCAGATCTCAATCAAAATGTATCATATCACACAGTTCCTCAAAGACTCCGATCAGCGTCGGCGGGATTCAAACCCGGGTGTGGGGGTTCCACACCTGGAAGCTCTTACCAACTCGACCTAAAAGCTTCTCTGTCAGGGAAATGGGGAGGCAACTGAATCTGCTTCTTATGCAAATGTGCTTGATTCTGGGTGTTTTGCCATTTCATGGATCTGCAGTATCAACATGTGGTTGTGTGCAGCCAATTGAAGTTAAACTCCAACTAGATGTTGCAATATACACATTCTTCCCTTCAGTTTCAAAGCTGGCTGAGGAAATCGCAGACAGTGTTGCACTAAACCATAGCCAAGTTCGCATAATGGGAGCAGATGCATCTAGTCAGCAGCTGGAGAAAACTGCCGTCCTCGTTAACTTGGTACCTATAGGACTAACATTTGATGGCACTACTGCATTTCAAATCTATGACAAATTCTGGCGCAGACAGGGTCTTCCACCTTCTCCCCCTTCAGCACCTTCAGCCATTTCTACTATAGATGACGGGCCATACATGGGCCAAGACAACAATGGAAGGGTAATAAAACCTATACCAGTTAATGTGCCAAGAAAAAGAAATGATGGAATCAAAGGAAGTATGCTTGCTGTGATCGTTCTATCATCTTTCACAGCCTTTCTTTTATGTGTGGGGTTTGTCTGGCTTTTGTTGCTGAAATGTCGATCTCACTCCCATAAGCCGGAACATTTTCCACAAGCTTTGACATCGTCCCCTACAAAACTCACAGGGGATCCCAGTGCTACTTTAGATGATGGACGAGAGGTGGCTGTGAAGGTTCTGAAGAGAGATGATCATCATGGTGGCCGCGAATTCTTAGCAGAAGTTGAGATGCTTGGCTGTCTTCACCACAGAAATTTGGTCAAATTAATTGGTATCTGCACAGAGGGCCATACTCGTTGCCTCGTTTACGAACTTGTTCCAAATGGAAGTGTTGAATGCCATTTACATGGTGTTGACAAGGAAACTGATCCACTTGATTGGGATGCCAGAATAAAGATTGCCCTTGGTGCAGCTCAGGGCTTAGCTTATCTCCACGAAGACTCAAATCCTCGAGTTATACATAGTGACTTCAAGGCCAGCAACATCTTACTGGAATATGATTTTACACCCAAGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

405

Amino Acids

44.36

Weight (kDa)

8.47

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	88 - 177	3.1e-38	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	283 - 400	1.5e-17	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	284 - 402	1.2e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42950 AT2G42950
fragaria_vesca	FvH4_6g38330
malus_domestica	MD17G1138500.v1.1
prunus_persica	Prupe.3G173900_v2.0.a1 Prupe.3G173900_v2.0.a1 Prupe.4G074700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0151641 RchiOBHm_Chr2g0151651
rosa_laevigata	RLG00000020566
rosa_multiflora	Rmu_co8346043.1_g000001 Rmu_sc0005287.1_g000001 Rmu_sc0029736.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096290
rosa_rugosa	Rorug02G0427500 Rorug02G0427600
rosa_samantha	Rh2AG488400 Rh2BG501000 Rh2BG501100 Rh2CG474900 Rh2DG512100 Rh2DG512200
rosa_wichuraiana	Rw2G040040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	463
AccB1I	GGYRCC	1 cut(s)	463
AccB7I	CCANNNNNTGG	1 cut(s)	254
AccII	CGCG	1 cut(s)	906
AciI	CCGC	2 cut(s)	111, 904
AclWI	GGATC	4 cut(s)	265, 824, 837, 1061
AcoI	YGGCCR	2 cut(s)	3, 901
AcsI	RAATTY	3 cut(s)	515, 908, 952
AcuI	CTGAAG	4 cut(s)	324, 537, 546, 899
AfaI	GTAC	1 cut(s)	465
AfiI	CCNNNNNNNGG	5 cut(s)	129, 130, 254, 596, 778
AflIII	ACRYGT	1 cut(s)	272
AgsI	TTSAA	6 cut(s)	119, 296, 348, 503, 1034, 1171
AjnI	CCWGG	1 cut(s)	143
AluBI	AGCT	7 cut(s)	151, 174, 352, 433, 797, 1112, 1124
AluI	AGCT	7 cut(s)	151, 174, 352, 433, 797, 1112, 1124
AlwI	GGATC	4 cut(s)	265, 824, 837, 1061
AlwNI	CAGNNNCTG	1 cut(s)	433
Ama87I	CYCGRG	2 cut(s)	123, 1149
AoxI	GGCC	7 cut(s)	3, 40, 587, 598, 901, 985, 1173
ApeKI	GCWGC	3 cut(s)	286, 430, 1109
ApoI	RAATTY	3 cut(s)	515, 908, 952
AseI	ATTAAT	1 cut(s)	965
Asp700I	GAANNNNTTC	2 cut(s)	786, 1014
Asp718I	GGTACC	1 cut(s)	463
AspLEI	GCGC	1 cut(s)	525
AspS9I	GGNCC	3 cut(s)	587, 598, 985
AsuC2I	CCSGG	2 cut(s)	124, 125
AsuHPI	GGTGA	1 cut(s)	935
AvaI	CYCGRG	2 cut(s)	123, 1149
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	829
BanI	GGYRCC	1 cut(s)	463
BbsI	GAAGAC	3 cut(s)	527, 932, 1143
BbvCI	CCTCAGC	1 cut(s)	357
BbvI	GCAGC	3 cut(s)	298, 442, 1121
BccI	CCATC	3 cut(s)	479, 656, 845
BceAI	ACGGC	1 cut(s)	431
BciT130I	CCWGG	1 cut(s)	145
BclI	TGATCA	1 cut(s)	889
BcnI	CCSGG	2 cut(s)	124, 125
BfaI	CTAG	2 cut(s)	311, 423
BfmI	CTRYAG	3 cut(s)	261, 468, 576
BglI	GCCNNNNNGGC	1 cut(s)	48
BglII	AGATCT	1 cut(s)	53
BisI	GCNGC	4 cut(s)	287, 431, 904, 1110
BlpI	GCTNAGC	1 cut(s)	1120
BlsI	GCNGC	4 cut(s)	288, 432, 905, 1111
Bme1390I	CCNGG	3 cut(s)	124, 125, 145
BmeT110I	CYCGRG	2 cut(s)	123, 1149
BmgT120I	GGNCC	3 cut(s)	587, 598, 985
BmiI	GGNNCC	3 cut(s)	137, 465, 831
BmrFI	CCNGG	3 cut(s)	124, 125, 145
BmrI	ACTGGG	1 cut(s)	828
BmsI	GCATC	4 cut(s)	406, 428, 918, 1072
BmuI	ACTGGG	1 cut(s)	828
BpiI	GAAGAC	3 cut(s)	527, 932, 1143
BpmI	CTGGAG	1 cut(s)	455
Bpu10I	CCTNAGC	2 cut(s)	357, 1113
Bpu1102I	GCTNAGC	1 cut(s)	1120
BpuEI	CTTGAG	1 cut(s)	21
BpuMI	CCSGG	2 cut(s)	124, 125
BsaBI	GATNNNNATC	2 cut(s)	58, 891
BsaJI	CCNNGG	3 cut(s)	123, 1102, 1209
Bsc4I	CCNNNNNNNGG	5 cut(s)	129, 130, 254, 596, 778
Bse1I	ACTGG	3 cut(s)	635, 834, 1194
Bse8I	GATNNNNATC	2 cut(s)	58, 891
BseBI	CCWGG	1 cut(s)	145
BseDI	CCNNGG	3 cut(s)	123, 1102, 1209
BseGI	GGATG	1 cut(s)	1087
BseJI	GATNNNNATC	2 cut(s)	58, 891
BseLI	CCNNNNNNNGG	5 cut(s)	129, 130, 254, 596, 778
BseMII	CTCAG	2 cut(s)	348, 1127
BseNI	ACTGG	3 cut(s)	635, 834, 1194
BseXI	GCAGC	3 cut(s)	298, 442, 1121
BsgI	GTGCAG	3 cut(s)	305, 961, 1128
Bsh1236I	CGCG	1 cut(s)	906
BshFI	GGCC	7 cut(s)	5, 42, 589, 600, 903, 987, 1175
BshNI	GGYRCC	1 cut(s)	463
BsiHKCI	CYCGRG	2 cut(s)	123, 1149
BsiSI	CCGG	2 cut(s)	124, 779
BslFI	GGGAC	1 cut(s)	793
BslI	CCNNNNNNNGG	5 cut(s)	129, 130, 254, 596, 778
BsmFI	GGGAC	1 cut(s)	793
BsmI	GAATGC	1 cut(s)	1040
BsnI	GGCC	7 cut(s)	5, 42, 589, 600, 903, 987, 1175
BsoBI	CYCGRG	2 cut(s)	123, 1149
Bsp143I	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
Bsp1720I	GCTNAGC	1 cut(s)	1120
BspACI	CCGC	2 cut(s)	111, 904
BspANI	GGCC	7 cut(s)	5, 42, 589, 600, 903, 987, 1175
BspCNI	CTCAG	2 cut(s)	349, 1126
BspFNI	CGCG	1 cut(s)	906
BspLI	GGNNCC	3 cut(s)	137, 465, 831
BspMAI	CTGCAG	1 cut(s)	265
BspPI	GGATC	4 cut(s)	265, 824, 837, 1061
BspT107I	GGYRCC	1 cut(s)	463
BsrI	ACTGG	3 cut(s)	635, 834, 1194
BssECI	CCNNGG	3 cut(s)	123, 1102, 1209
BssMI	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
BssT1I	CCWWGG	2 cut(s)	1102, 1209
Bst2UI	CCWGG	1 cut(s)	145
Bst4CI	ACNGT	2 cut(s)	83, 374
Bst6I	CTCTTC	1 cut(s)	875
BstC8I	GCNNGC	3 cut(s)	354, 684, 1177
BstDEI	CTNAG	4 cut(s)	357, 913, 1113, 1120
BstF5I	GGATG	1 cut(s)	1087
BstFNI	CGCG	1 cut(s)	906
BstHHI	GCGC	1 cut(s)	525
BstKTI	GATC	8 cut(s)	56, 102, 260, 693, 764, 832, 892, 1069
BstMBI	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
BstMWI	GCNNNNNNNGC	7 cut(s)	48, 217, 399, 563, 748, 984, 1106
BstNI	CCWGG	1 cut(s)	145
BstNSI	RCATGY	1 cut(s)	276
BstSCI	CCNGG	3 cut(s)	122, 123, 143
BstSFI	CTRYAG	3 cut(s)	261, 468, 576
BstUI	CGCG	1 cut(s)	906
BstV1I	GCAGC	3 cut(s)	298, 442, 1121
BstV2I	GAAGAC	3 cut(s)	527, 932, 1143
BstX2I	RGATCY	3 cut(s)	53, 257, 829
BstXI	CCANNNNNNTGG	2 cut(s)	1046, 1077
BstYI	RGATCY	3 cut(s)	53, 257, 829
BsuRI	GGCC	7 cut(s)	5, 42, 589, 600, 903, 987, 1175
BtsCI	GGATG	1 cut(s)	1087
BtsIMutI	CAGTG	2 cut(s)	379, 841
Cac8I	GCNNGC	3 cut(s)	354, 684, 1177
CaiI	CAGNNNCTG	1 cut(s)	433
CfoI	GCGC	1 cut(s)	525
Cfr13I	GGNCC	3 cut(s)	587, 598, 985
Cfr9I	CCCGGG	1 cut(s)	123
CseI	GACGC	1 cut(s)	94
Csp6I	GTAC	1 cut(s)	464
CspCI	CAANNNNNGTGG	2 cut(s)	780, 815
CviAII	CATG	5 cut(s)	254, 273, 595, 896, 1046
CviQI	GTAC	1 cut(s)	464
DdeI	CTNAG	4 cut(s)	357, 913, 1113, 1120
DpnI	GATC	8 cut(s)	55, 101, 259, 692, 763, 831, 891, 1068
DpnII	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
EaeI	YGGCCR	2 cut(s)	3, 901
Eam1104I	CTCTTC	1 cut(s)	875
EarI	CTCTTC	1 cut(s)	875
Eco130I	CCWWGG	2 cut(s)	1102, 1209
Eco147I	AGGCCT	1 cut(s)	42
Eco57I	CTGAAG	4 cut(s)	324, 537, 546, 899
Eco88I	CYCGRG	2 cut(s)	123, 1149
EcoRI	GAATTC	1 cut(s)	908
EcoRII	CCWGG	1 cut(s)	143
EcoT14I	CCWWGG	2 cut(s)	1102, 1209
EcoT22I	ATGCAT	1 cut(s)	421
ErhI	CCWWGG	2 cut(s)	1102, 1209
FaeI	CATG	5 cut(s)	257, 276, 598, 899, 1049
FaqI	GGGAC	1 cut(s)	793
FatI	CATG	5 cut(s)	253, 272, 594, 895, 1045
FauI	CCCGC	1 cut(s)	104
FbaI	TGATCA	1 cut(s)	889
Fnu4HI	GCNGC	4 cut(s)	287, 431, 904, 1110
FokI	GGATG	1 cut(s)	1094
Fsp4HI	GCNGC	4 cut(s)	287, 431, 904, 1110
FspBI	CTAG	2 cut(s)	311, 423
GlaI	GCGC	1 cut(s)	524
GluI	GCNGC	4 cut(s)	287, 431, 904, 1110
GsuI	CTGGAG	1 cut(s)	455
HaeIII	GGCC	7 cut(s)	5, 42, 589, 600, 903, 987, 1175
HapII	CCGG	2 cut(s)	124, 779
HgaI	GACGC	1 cut(s)	94
HhaI	GCGC	1 cut(s)	525
Hin1II	CATG	5 cut(s)	257, 276, 598, 899, 1049
Hin6I	GCGC	1 cut(s)	523
HinP1I	GCGC	1 cut(s)	523
HincII	GTYRAC	2 cut(s)	457, 1054
HindII	GTYRAC	2 cut(s)	457, 1054
HindIII	AAGCTT	2 cut(s)	172, 795
HinfI	GANTC	6 cut(s)	94, 115, 205, 232, 666, 1139
HpaI	GTTAAC	1 cut(s)	457
HpaII	CCGG	2 cut(s)	124, 779
HphI	GGTGA	1 cut(s)	935
Hpy166II	GTNNAC	3 cut(s)	457, 1006, 1054
Hpy188I	TCNGA	2 cut(s)	99, 879
Hpy8I	GTNNAC	3 cut(s)	457, 1006, 1054
Hpy99I	CGWCG	1 cut(s)	110
HpyAV	CCTTC	6 cut(s)	348, 552, 561, 570, 609, 865
HpyCH4III	ACNGT	2 cut(s)	83, 374
HpyCH4V	TGCA	9 cut(s)	220, 263, 286, 320, 380, 419, 497, 978, 1109
HpyF10VI	GCNNNNNNNGC	7 cut(s)	48, 217, 399, 563, 748, 984, 1106
HpyF3I	CTNAG	4 cut(s)	357, 913, 1113, 1120
Hsp92II	CATG	5 cut(s)	257, 276, 598, 899, 1049
HspAI	GCGC	1 cut(s)	523
KpnI	GGTACC	1 cut(s)	467
Ksp22I	TGATCA	1 cut(s)	889
KspAI	GTTAAC	1 cut(s)	457
Kzo9I	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
LmnI	GCTCC	1 cut(s)	410
Lsp1109I	GCAGC	3 cut(s)	298, 442, 1121
LweI	GCATC	4 cut(s)	406, 428, 918, 1072
MaeI	CTAG	2 cut(s)	311, 423
MaeIII	GTNAC	1 cut(s)	1163
MalI	GATC	8 cut(s)	55, 101, 259, 692, 763, 831, 891, 1068
MboI	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
MboII	GAAGA	5 cut(s)	325, 527, 892, 932, 1148
MfeI	CAATTG	1 cut(s)	291
MflI	RGATCY	3 cut(s)	53, 257, 829
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	7 cut(s)	13, 291, 515, 908, 952, 962, 966
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	88, 1133
MmeI	TCCRAC	1 cut(s)	331
MnlI	CCTC	8 cut(s)	97, 189, 352, 461, 853, 976, 1010, 1158
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	421
MroXI	GAANNNNTTC	2 cut(s)	786, 1014
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	4 cut(s)	300, 456, 639, 965
MslI	CAYNNNNRTG	2 cut(s)	897, 1044
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	433
MspI	CCGG	2 cut(s)	124, 779
MspR9I	CCNGG	3 cut(s)	124, 125, 145
MunI	CAATTG	1 cut(s)	291
Mva1269I	GAATGC	1 cut(s)	1040
MvaI	CCWGG	1 cut(s)	145
MvnI	CGCG	1 cut(s)	906
MwoI	GCNNNNNNNGC	7 cut(s)	48, 217, 399, 563, 748, 984, 1106
NciI	CCSGG	2 cut(s)	124, 125
NdeII	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
NlaIII	CATG	5 cut(s)	257, 276, 598, 899, 1049
NlaIV	GGNNCC	3 cut(s)	137, 465, 831
NmuCI	GTSAC	1 cut(s)	1163
NsiI	ATGCAT	1 cut(s)	421
NspI	RCATGY	1 cut(s)	276
PaeR7I	CTCGAG	1 cut(s)	1149
PceI	AGGCCT	1 cut(s)	42
PciI	ACATGT	1 cut(s)	272
PctI	GAATGC	1 cut(s)	1040
PdmI	GAANNNNTTC	2 cut(s)	786, 1014
PfeI	GAWTC	4 cut(s)	115, 205, 232, 666
PflFI	GACNNNGTC	2 cut(s)	531, 805
PflMI	CCANNNNNTGG	1 cut(s)	254
PkrI	GCNGC	4 cut(s)	288, 432, 905, 1111
PleI	GAGTC	2 cut(s)	88, 1133
PpsI	GAGTC	2 cut(s)	88, 1133
PscI	ACATGT	1 cut(s)	272
PshBI	ATTAAT	1 cut(s)	965
Psp6I	CCWGG	1 cut(s)	143
PspGI	CCWGG	1 cut(s)	143
PspN4I	GGNNCC	3 cut(s)	137, 465, 831
PspPI	GGNCC	3 cut(s)	587, 598, 985
PspXI	VCTCGAGB	1 cut(s)	1149
PstI	CTGCAG	1 cut(s)	265
PstNI	CAGNNNCTG	1 cut(s)	433
PsuI	RGATCY	3 cut(s)	53, 257, 829
PsyI	GACNNNGTC	2 cut(s)	531, 805
PvuII	CAGCTG	1 cut(s)	433
RsaI	GTAC	1 cut(s)	465
RsaNI	GTAC	1 cut(s)	464
RseI	CAYNNNNRTG	2 cut(s)	897, 1044
SaqAI	TTAA	4 cut(s)	300, 456, 639, 965
SatI	GCNGC	4 cut(s)	287, 431, 904, 1110
Sau3AI	GATC	8 cut(s)	53, 99, 257, 690, 761, 829, 889, 1066
Sau96I	GGNCC	3 cut(s)	587, 598, 985
SchI	GAGTC	2 cut(s)	88, 1133
ScrFI	CCNGG	3 cut(s)	124, 125, 145
SfaNI	GCATC	4 cut(s)	406, 428, 918, 1072
SfcI	CTRYAG	3 cut(s)	261, 468, 576
Sfr274I	CTCGAG	1 cut(s)	1149
SlaI	CTCGAG	1 cut(s)	1149
SmaI	CCCGGG	1 cut(s)	125
SmiMI	CAYNNNNRTG	2 cut(s)	897, 1044
SmlI	CTYRAG	2 cut(s)	36, 1149
SmoI	CTYRAG	2 cut(s)	36, 1149
Sse9I	AATT	7 cut(s)	13, 291, 515, 908, 952, 962, 966
SseBI	AGGCCT	1 cut(s)	42
SsiI	CCGC	2 cut(s)	111, 904
SspMI	CTAG	2 cut(s)	311, 423
StuI	AGGCCT	1 cut(s)	42
StyD4I	CCNGG	3 cut(s)	122, 123, 143
StyI	CCWWGG	2 cut(s)	1102, 1209
TaaI	ACNGT	2 cut(s)	83, 374
TaqI	TCGA	3 cut(s)	163, 760, 1150
TasI	AATT	7 cut(s)	13, 291, 515, 908, 952, 962, 966
TauI	GCSGC	1 cut(s)	906
TfiI	GAWTC	4 cut(s)	115, 205, 232, 666
Tru1I	TTAA	4 cut(s)	300, 456, 639, 965
Tru9I	TTAA	4 cut(s)	300, 456, 639, 965
TscAI	CASTG	2 cut(s)	379, 841
TseFI	GTSAC	1 cut(s)	1163
TseI	GCWGC	3 cut(s)	286, 430, 1109
Tsp45I	GTSAC	1 cut(s)	1163
TspDTI	ATGAA	1 cut(s)	242
TspMI	CCCGGG	1 cut(s)	123
TspRI	CASTG	2 cut(s)	379, 841
Tth111I	GACNNNGTC	2 cut(s)	531, 805
Van91I	CCANNNNNTGG	1 cut(s)	254
VspI	ATTAAT	1 cut(s)	965
XapI	RAATTY	3 cut(s)	515, 908, 952
XceI	RCATGY	1 cut(s)	276
XcmI	CCANNNNNNNNNTGG	1 cut(s)	608
XhoI	CTCGAG	1 cut(s)	1149
XmaI	CCCGGG	1 cut(s)	123
XmnI	GAANNNNTTC	2 cut(s)	786, 1014
XspI	CTAG	2 cut(s)	311, 423
Zsp2I	ATGCAT	1 cut(s)	421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.