Rorug02G0478400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009058
Physical Location & Seq
Reverse (-)
60973046 .. 60974935
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0478400.1

Sequence Viewer

Length: 1890 bp
ATGTCTACTTCATCATCAAAAGATGCAGAGAAATGCATTCCCAAGTTGCTGGGTCGTCTCAGCGAAGCCCGGACGGCGGCCAACACCGACGATCCGGCGGTTCTAAATAGCTTCCAAAGGATCCAAGAAGAGCTTGAAATCATGAAAAACATGCTACTGCTACCCAGAGCCAAACAGTGGGAGCAAACTCTGTCAGACCAGTTCAATGCCTTGGAACGCCGCCTCGACAAGTTCATCTTGGGATCCCAGGACGGCGAGTCCATGAGCAGCGTGGCAATCAACCGAGTGCTGGATCCCATTTTCGAAAACGTCGAAAACATCAAACTCGCAATCCCTTTGGTGGAGTCGATGCCTTCATTTCAACACGTTCCTGAGTCATGGACGCGTTCGTCTCAGAGTCATCAAGCAGCTCAGAAAAAGTCACAGGTGTGGTCTCAACTGGACGTGGAGTATGGGCTGTACGAGAGTGCAGCCATGAGTAGTATAAAGCTGAGCTATGAGCGTTTAGAGAGCATCGAACTGAAGATGTGCTGTCTATCTCTGTCGATCTTTCCAGAGAGTTCTGTCATAAAGAAGAGGCCTCTAATTTACTTCTGGATTGGCCAGGGTTTCGTTACAAGCACACAAGACGAGACTGCAGAGGAGGTAGGAGAAGAAGTCTTTAGAAAACTCATGACATGCAACTTGATTCAACCACATTTAAATGCGTCCAGTACATTTGTGAATAATTGTACAATACATCCTTGGATTCATCGCATGTTGATGTTCTTGGCTCATGAAGCTGAGCTTTTCGACTTTAGTTCAAGATTGCCATTGAAGGAAACTATGAAAAATAGTATGGGTGTATGCTTAACTTTTGATAAGCCAGTCCCTGAGTTACCTCCAGCTGACTTTGACAATGTTATACCGACAGTTTTCAATGTAAGTGCCCAATATCTCATACTGAAACCAGAATGGCTAATCGGGTTGAAGACACTGACGGTTCTCCAGCTGGGACGGTGGCAGAACTCGACTTCTCATCACATTGAAGTGGATGATGAAGCATTTTTAGATGGATTAGGAGCCCTGAAGCACTTGAAGTATTTCAGCCTCCGCGGAATGTCAACAATTGCCAAGCTCCCTTCAGCTCTCCTTAAGCTTATCAACCTTGAAATTCTAGATCTGAGAGCATGTCACAACTTGGAGAAATTGCCTTCGGATATCTCATCGTTGAAAAAGCTCACACATTTGGATGTGTCAGAGTGTTACTTGCTTGACATTATGCCAAAAGCGATTGACAAGCTCTCTTGCCTTGAAGTTCTGGAAGGTTATGTGATAGGAGGTCCAAAAAGAACTCCCTGCAGAGTTGGTGATCTAGCCAAATTGAAGAAACTAAAGCGGCTCAGCATACACATGGGGAATGAGGCTGTGGTTAAAGATGGGGAGTTGGAAGAGTTGGAGAAAATTGAATCTCTTCGCCGCCTCAAAGTATCATGGGGAGTGGTTGCTCCAAAGTTGAGGAAAAAGGTCTCTGAACAGTCCTTGAAACTCTCATTTCCACCGGATTTAGAAAAATTGGAACTTCAAGGCATCCCCCTAGGACAAGTACCTGAATGGTTGAAGCCTAGCAAATTGAAGAAGTTAAAGAAGCTCCACATTAGGGGTGGGATGCTTGATAGTTTAAATCACAACGAGACTGAAGAGTGGAAAGTTGAAGTATTGCGTCTCAAGTACTTGAAGAAGCTGGATATAGGATTTCAACAGTTGCGGCAAGAGTTTCCTCATCTGCGGTACTTGGAGAAAATCGGCTGCCATCTTTGTCAAGGGGATACATATGATAAAGACATTGTTTGGAGCAAAGTGGAAGGTGATATATCAGATAGGTATATCAATGCTACGAATAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008150 GO:0009888 GO:0010087 GO:0010089 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

629

Amino Acids

71.64

Weight (kDa)

6.98

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 183 - 253 2.9e-16 Disease resistance protein Winged helix domain
LRR_14 PF23598 324 - 547 7.3e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 324 - 415 4.6e-07 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 451 - 577 1e-05 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 177
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 385, 1095
AciI CCGC 9 cut(s) 77, 98, 220, 1093, 1095, 1378, 1459, 1747, 1768
AclWI GGATC 7 cut(s) 86, 115, 128, 237, 250, 287, 300
AcoI YGGCCR 2 cut(s) 78, 601
AcsI RAATTY 1 cut(s) 1152
AcuI CTGAAG 4 cut(s) 542, 1088, 1107, 1698
AfaI GTAC 6 cut(s) 461, 715, 733, 1587, 1712, 1772
AfiI CCNNNNNNNGG 5 cut(s) 76, 177, 289, 340, 1639
AflII CTTAAG 1 cut(s) 1133
AflIII ACRYGT 2 cut(s) 364, 383
AhdI GACNNNNNGTC 1 cut(s) 256
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 2 cut(s) 246, 603
AleI CACNNNNGTG 1 cut(s) 427
Alw26I GTCTC 7 cut(s) 62, 396, 438, 626, 1513, 1667, 1709
AlwI GGATC 7 cut(s) 86, 115, 128, 237, 250, 287, 300
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 3 cut(s) 78, 578, 601
ApeKI GCWGC 4 cut(s) 267, 407, 470, 1788
ApoI RAATTY 1 cut(s) 1152
Asp700I GAANNNNTTC 2 cut(s) 1082, 1452
AspA2I CCTAGG 1 cut(s) 1576
AspS9I GGNCC 1 cut(s) 1322
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 1361, 1859
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 1 cut(s) 1322
AvrII CCTAGG 1 cut(s) 1576
BaeGI GKGCMC 1 cut(s) 931
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 3 cut(s) 120, 242, 292
BanII GRGCYC 1 cut(s) 1066
BbsI GAAGAC 1 cut(s) 977
BbvI GCAGC 4 cut(s) 279, 419, 482, 1775
BccI CCATC 3 cut(s) 1046, 1412, 1800
BceAI ACGGC 2 cut(s) 90, 268
BciT130I CCWGG 2 cut(s) 248, 605
BciVI GTATCC 1 cut(s) 1801
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 7 cut(s) 62, 396, 438, 626, 1513, 1667, 1709
BfaI CTAG 5 cut(s) 1157, 1355, 1577, 1605, 1888
BfmI CTRYAG 2 cut(s) 636, 1339
BfrI CTTAAG 1 cut(s) 1133
BfuI GTATCC 1 cut(s) 1801
BglI GCCNNNNNGGC 1 cut(s) 74
BglII AGATCT 1 cut(s) 1159
BisI GCNGC 9 cut(s) 78, 220, 268, 408, 471, 1379, 1459, 1748, 1789
BlnI CCTAGG 1 cut(s) 1576
BlpI GCTNAGC 3 cut(s) 491, 783, 1382
BlsI GCNGC 9 cut(s) 79, 221, 269, 409, 472, 1380, 1460, 1749, 1790
BmcAI AGTACT 1 cut(s) 1712
Bme1390I CCNGG 3 cut(s) 70, 248, 605
Bme18I GGWCC 1 cut(s) 1322
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 1322
BmiI GGNNCC 4 cut(s) 122, 244, 294, 1063
BmrFI CCNGG 3 cut(s) 70, 248, 605
BmsI GCATC 5 cut(s) 13, 339, 522, 1578, 1638
BpiI GAAGAC 1 cut(s) 977
BpmI CTGGAG 2 cut(s) 867, 971
Bpu1102I GCTNAGC 3 cut(s) 491, 783, 1382
Bpu14I TTCGAA 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 1691
BpuMI CCSGG 1 cut(s) 70
BsaI GGTCTC 2 cut(s) 438, 1513
BsaJI CCNNGG 6 cut(s) 210, 246, 604, 743, 1093, 1576
BsaWI WCCGGW 1 cut(s) 1540
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 177, 289, 340, 1639
Bse1I ACTGG 4 cut(s) 199, 444, 711, 866
BseBI CCWGG 2 cut(s) 248, 605
BseDI CCNNGG 6 cut(s) 210, 246, 604, 743, 1093, 1576
BseGI GGATG 5 cut(s) 739, 1039, 1237, 1569, 1653
BseLI CCNNNNNNNGG 5 cut(s) 76, 177, 289, 340, 1639
BseMII CTCAG 9 cut(s) 73, 363, 407, 425, 482, 774, 864, 1154, 1396
BseNI ACTGG 4 cut(s) 199, 444, 711, 866
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 4 cut(s) 279, 419, 482, 1775
BseYI CCCAGC 2 cut(s) 49, 991
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 2 cut(s) 385, 1095
BshFI GGCC 3 cut(s) 80, 580, 603
BsiSI CCGG 3 cut(s) 70, 95, 1541
BslFI GGGAC 2 cut(s) 854, 1008
BslI CCNNNNNNNGG 5 cut(s) 76, 177, 289, 340, 1639
BsmAI GTCTC 7 cut(s) 62, 396, 438, 626, 1513, 1667, 1709
BsmBI CGTCTC 3 cut(s) 62, 396, 1709
BsmFI GGGAC 2 cut(s) 854, 1008
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 3 cut(s) 80, 580, 603
Bso31I GGTCTC 2 cut(s) 438, 1513
Bsp119I TTCGAA 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 931, 1066
Bsp1407I TGTACA 1 cut(s) 731
Bsp143I GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
Bsp1720I GCTNAGC 3 cut(s) 491, 783, 1382
BspACI CCGC 9 cut(s) 77, 98, 220, 1093, 1095, 1378, 1459, 1747, 1768
BspANI GGCC 3 cut(s) 80, 580, 603
BspCNI CTCAG 9 cut(s) 72, 364, 406, 424, 483, 775, 865, 1155, 1395
BspFNI CGCG 2 cut(s) 385, 1095
BspHI TCATGA 3 cut(s) 141, 672, 775
BspLI GGNNCC 4 cut(s) 122, 244, 294, 1063
BspMAI CTGCAG 2 cut(s) 640, 1343
BspPI GGATC 7 cut(s) 86, 115, 128, 237, 250, 287, 300
BspQI GCTCTTC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 1133
BspTNI GGTCTC 2 cut(s) 438, 1513
BsrGI TGTACA 1 cut(s) 731
BsrI ACTGG 4 cut(s) 199, 444, 711, 866
BssECI CCNNGG 6 cut(s) 210, 246, 604, 743, 1093, 1576
BssMI GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
BssT1I CCWWGG 3 cut(s) 210, 743, 1576
Bst2UI CCWGG 2 cut(s) 248, 605
Bst4CI ACNGT 6 cut(s) 177, 913, 982, 999, 1518, 1743
Bst6I CTCTTC 5 cut(s) 123, 569, 1425, 1458, 1674
BstAFI CTTAAG 1 cut(s) 1133
BstAUI TGTACA 1 cut(s) 731
BstBI TTCGAA 1 cut(s) 303
BstDEI CTNAG 9 cut(s) 59, 372, 393, 411, 491, 783, 873, 1163, 1382
BstDSI CCRYGG 1 cut(s) 1093
BstF5I GGATG 5 cut(s) 739, 1039, 1237, 1569, 1653
BstFNI CGCG 2 cut(s) 385, 1095
BstKTI GATC 7 cut(s) 94, 123, 245, 295, 549, 1162, 1354
BstMAI GTCTC 7 cut(s) 62, 396, 438, 626, 1513, 1667, 1709
BstMBI GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
BstMWI GCNNNNNNNGC 2 cut(s) 74, 779
BstNI CCWGG 2 cut(s) 248, 605
BstNSI RCATGY 4 cut(s) 154, 681, 760, 1173
BstSCI CCNGG 3 cut(s) 68, 246, 603
BstSFI CTRYAG 2 cut(s) 636, 1339
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 2 cut(s) 385, 1095
BstV1I GCAGC 4 cut(s) 279, 419, 482, 1775
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 4 cut(s) 120, 242, 292, 1159
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 4 cut(s) 120, 242, 292, 1159
BsuI GTATCC 1 cut(s) 1801
BsuRI GGCC 3 cut(s) 80, 580, 603
BtgI CCRYGG 1 cut(s) 1093
BtgZI GCGATG 1 cut(s) 737
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 5 cut(s) 739, 1039, 1237, 1569, 1653
BtsIMutI CAGTG 2 cut(s) 182, 974
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 3 cut(s) 141, 672, 775
Cfr13I GGNCC 1 cut(s) 1322
Cfr42I CCGCGG 1 cut(s) 1096
CseI GACGC 3 cut(s) 391, 696, 1691
Csp6I GTAC 6 cut(s) 460, 714, 732, 1586, 1711, 1771
CviQI GTAC 6 cut(s) 460, 714, 732, 1586, 1711, 1771
DdeI CTNAG 9 cut(s) 59, 372, 393, 411, 491, 783, 873, 1163, 1382
DpnI GATC 7 cut(s) 93, 122, 244, 294, 548, 1161, 1353
DpnII GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
DraI TTTAAA 2 cut(s) 702, 1662
DrdI GACNNNNNNGTC 1 cut(s) 388
DriI GACNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 78, 601
Eam1104I CTCTTC 5 cut(s) 123, 569, 1425, 1458, 1674
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 5 cut(s) 123, 569, 1425, 1458, 1674
Eco130I CCWWGG 3 cut(s) 210, 743, 1576
Eco147I AGGCCT 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 1066
Eco31I GGTCTC 2 cut(s) 438, 1513
Eco32I GATATC 1 cut(s) 1201
Eco47I GGWCC 1 cut(s) 1322
Eco57I CTGAAG 4 cut(s) 542, 1088, 1107, 1698
EcoRII CCWGG 2 cut(s) 246, 603
EcoRV GATATC 1 cut(s) 1201
EcoT14I CCWWGG 3 cut(s) 210, 743, 1576
EcoT22I ATGCAT 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 1066
ErhI CCWWGG 3 cut(s) 210, 743, 1576
Esp3I CGTCTC 3 cut(s) 62, 396, 1709
FalI AAGNNNNNCTT 6 cut(s) 117, 149, 221, 253, 771, 803
FaqI GGGAC 2 cut(s) 854, 1008
FauNDI CATATG 1 cut(s) 1813
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 9 cut(s) 78, 220, 268, 408, 471, 1379, 1459, 1748, 1789
FokI GGATG 5 cut(s) 726, 1046, 1244, 1556, 1660
FriOI GRGCYC 1 cut(s) 1066
Fsp4HI GCNGC 9 cut(s) 78, 220, 268, 408, 471, 1379, 1459, 1748, 1789
FspBI CTAG 5 cut(s) 1157, 1355, 1577, 1605, 1888
GluI GCNGC 9 cut(s) 78, 220, 268, 408, 471, 1379, 1459, 1748, 1789
GsaI CCCAGC 2 cut(s) 53, 995
GsuI CTGGAG 2 cut(s) 867, 971
HaeIII GGCC 3 cut(s) 80, 580, 603
HapII CCGG 3 cut(s) 70, 95, 1541
HgaI GACGC 3 cut(s) 391, 696, 1691
HincII GTYRAC 1 cut(s) 1104
HindII GTYRAC 1 cut(s) 1104
HindIII AAGCTT 1 cut(s) 1136
HinfI GANTC 7 cut(s) 257, 344, 374, 397, 688, 748, 1448
HpaII CCGG 3 cut(s) 70, 95, 1541
HphI GGTGA 2 cut(s) 1361, 1859
Hpy166II GTNNAC 2 cut(s) 6, 1104
Hpy188I TCNGA 8 cut(s) 196, 396, 414, 1164, 1198, 1240, 1513, 1858
Hpy188III TCNNGA 9 cut(s) 142, 371, 554, 595, 673, 776, 804, 1157, 1301
Hpy8I GTNNAC 2 cut(s) 6, 1104
Hpy99I CGWCG 2 cut(s) 92, 314
HpyAV CCTTC 6 cut(s) 363, 811, 1131, 1203, 1298, 1838
HpyCH4III ACNGT 6 cut(s) 177, 913, 982, 999, 1518, 1743
HpyCH4IV ACGT 3 cut(s) 309, 366, 444
HpyCH4V TGCA 6 cut(s) 26, 36, 470, 638, 681, 1341
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 779
HpyF3I CTNAG 9 cut(s) 59, 372, 393, 411, 491, 783, 873, 1163, 1382
HpySE526I ACGT 3 cut(s) 309, 366, 444
KspI CCGCGG 1 cut(s) 1096
Kzo9I GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 6 cut(s) 181, 1061, 1122, 1492, 1635, 1833
Lsp1109I GCAGC 4 cut(s) 279, 419, 482, 1775
LweI GCATC 5 cut(s) 13, 339, 522, 1578, 1638
MaeI CTAG 5 cut(s) 1157, 1355, 1577, 1605, 1888
MaeII ACGT 3 cut(s) 309, 366, 444
MaeIII GTNAC 5 cut(s) 420, 613, 876, 1172, 1244
MalI GATC 7 cut(s) 93, 122, 244, 294, 548, 1161, 1353
MboI GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
MfeI CAATTG 1 cut(s) 1107
MflI RGATCY 4 cut(s) 120, 242, 292, 1159
MhlI GDGCHC 2 cut(s) 931, 1066
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 9 cut(s) 585, 727, 1107, 1152, 1187, 1361, 1443, 1553, 1610
MluI ACGCGT 1 cut(s) 383
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 4 cut(s) 266, 353, 383, 406
MmeI TCCRAC 2 cut(s) 1407, 1416
Mox20I TGGCCA 1 cut(s) 603
Mph1103I ATGCAT 1 cut(s) 38
MroXI GAANNNNTTC 2 cut(s) 1082, 1452
MscI TGGCCA 1 cut(s) 603
MseI TTAA 6 cut(s) 701, 851, 1134, 1413, 1622, 1661
MslI CAYNNNNRTG 6 cut(s) 427, 702, 761, 1028, 1230, 1391
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 3 cut(s) 887, 991, 1095
MspCI CTTAAG 1 cut(s) 1133
MspI CCGG 3 cut(s) 70, 95, 1541
MspR9I CCNGG 3 cut(s) 70, 248, 605
MunI CAATTG 1 cut(s) 1107
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 2 cut(s) 248, 605
MvnI CGCG 2 cut(s) 385, 1095
MwoI GCNNNNNNNGC 2 cut(s) 74, 779
NciI CCSGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 1813
NdeII GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
NlaIV GGNNCC 4 cut(s) 122, 244, 294, 1063
NmuCI GTSAC 2 cut(s) 420, 1172
NsiI ATGCAT 1 cut(s) 38
NspI RCATGY 4 cut(s) 154, 681, 760, 1173
NspV TTCGAA 1 cut(s) 303
OliI CACNNNNGTG 1 cut(s) 427
PagI TCATGA 3 cut(s) 141, 672, 775
PceI AGGCCT 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 1082, 1452
PfeI GAWTC 3 cut(s) 688, 748, 1448
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 9 cut(s) 79, 221, 269, 409, 472, 1380, 1460, 1749, 1790
PleI GAGTC 4 cut(s) 265, 352, 382, 405
PpsI GAGTC 4 cut(s) 265, 352, 382, 405
Psp6I CCWGG 2 cut(s) 246, 603
PspFI CCCAGC 2 cut(s) 49, 991
PspGI CCWGG 2 cut(s) 246, 603
PspN4I GGNNCC 4 cut(s) 122, 244, 294, 1063
PspPI GGNCC 1 cut(s) 1322
PstI CTGCAG 2 cut(s) 640, 1343
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 4 cut(s) 120, 242, 292, 1159
PvuII CAGCTG 2 cut(s) 887, 991
RsaI GTAC 6 cut(s) 461, 715, 733, 1587, 1712, 1772
RsaNI GTAC 6 cut(s) 460, 714, 732, 1586, 1711, 1771
RseI CAYNNNNRTG 6 cut(s) 427, 702, 761, 1028, 1230, 1391
SacII CCGCGG 1 cut(s) 1096
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 6 cut(s) 701, 851, 1134, 1413, 1622, 1661
SatI GCNGC 9 cut(s) 78, 220, 268, 408, 471, 1379, 1459, 1748, 1789
Sau3AI GATC 7 cut(s) 91, 120, 242, 292, 546, 1159, 1351
Sau96I GGNCC 1 cut(s) 1322
ScaI AGTACT 1 cut(s) 1712
SchI GAGTC 4 cut(s) 266, 353, 383, 406
ScrFI CCNGG 3 cut(s) 70, 248, 605
SduI GDGCHC 2 cut(s) 931, 1066
SfaNI GCATC 5 cut(s) 13, 339, 522, 1578, 1638
SfcI CTRYAG 2 cut(s) 636, 1339
Sfr303I CCGCGG 1 cut(s) 1096
SfuI TTCGAA 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 1096
SinI GGWCC 1 cut(s) 1322
SmiI ATTTAAAT 1 cut(s) 702
SmiMI CAYNNNNRTG 6 cut(s) 427, 702, 761, 1028, 1230, 1391
SmlI CTYRAG 2 cut(s) 1133, 1706
SmoI CTYRAG 2 cut(s) 1133, 1706
Sse9I AATT 9 cut(s) 585, 727, 1107, 1152, 1187, 1361, 1443, 1553, 1610
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 9 cut(s) 77, 98, 220, 1093, 1095, 1378, 1459, 1747, 1768
SspMI CTAG 5 cut(s) 1157, 1355, 1577, 1605, 1888
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 3 cut(s) 68, 246, 603
StyI CCWWGG 3 cut(s) 210, 743, 1576
SwaI ATTTAAAT 1 cut(s) 702
TaaI ACNGT 6 cut(s) 177, 913, 982, 999, 1518, 1743
TaiI ACGT 3 cut(s) 312, 369, 447
TaqI TCGA 8 cut(s) 225, 303, 312, 347, 516, 545, 792, 1010
TasI AATT 9 cut(s) 585, 727, 1107, 1152, 1187, 1361, 1443, 1553, 1610
TatI WGTACW 3 cut(s) 713, 731, 1710
TauI GCSGC 5 cut(s) 80, 222, 1381, 1461, 1750
TfiI GAWTC 3 cut(s) 688, 748, 1448
Tru1I TTAA 6 cut(s) 701, 851, 1134, 1413, 1622, 1661
Tru9I TTAA 6 cut(s) 701, 851, 1134, 1413, 1622, 1661
TscAI CASTG 2 cut(s) 182, 981
TseFI GTSAC 2 cut(s) 420, 1172
TseI GCWGC 4 cut(s) 267, 407, 470, 1788
Tsp45I GTSAC 2 cut(s) 420, 1172
TspDTI ATGAA 7 cut(s) 158, 223, 345, 740, 792, 842, 1053
TspRI CASTG 2 cut(s) 182, 981
Van91I CCANNNNNTGG 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 1133
VpaK11BI GGWCC 1 cut(s) 1322
XapI RAATTY 1 cut(s) 1152
XbaI TCTAGA 1 cut(s) 1156
XceI RCATGY 4 cut(s) 154, 681, 760, 1173
XcmI CCANNNNNNNNNTGG 2 cut(s) 268, 1640
XmaJI CCTAGG 1 cut(s) 1576
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 1082, 1452
XspI CTAG 5 cut(s) 1157, 1355, 1577, 1605, 1888
ZrmI AGTACT 1 cut(s) 1712
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.