Rorug02G0535900

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008793
Physical Location & Seq
Reverse (-)
66470228 .. 66471156
929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0535900.1

Sequence Viewer

Length: 921 bp
ATGGAGAGAGAGAGCCTGATGTCACTGCTTGTGAACGTGAAGGATCAGGAGAGCAGCAGCAAGTCGCCGGAGGAAACTCAGGCCACCTTCACCGGCATGTTCCCCGTCAACCTCCATTCCATCCTCGCCGCCAACACCACCCAGCCCTTACTCCCTCTCTCCTTGTTCGCCGATAACTACTGCAGCCTCACCGCTCCCAAAACCGAACCGCCGCTCTACCACCACCACCAGCCACCACAATACGGCGCCGTTTCGGCTCAGACGTCGTCCCTCAACGACTACTACTCCATCTTCAACCACCTCCCGGACCTCCTCTCCCTCGACCACTCTCCCTACAAGCGCCCTCGCCTCCTCGACCACCAAACCCTAGCCTCCTCCTCCTACCAGCTCTACCCGACGCCGCCAGAATCTCAGCCGGCTCCGGTGGTGGTGCCACGTGGAAGCAGCCTGGCGCGGAAGAGGCGGCAGACGCTGAGCGACAAGATGCGGTGCCTGCAGAAGCTGCTGCCGTGGGAGAAGAAGATGGACAACGCCACCATGCTCGAGGCGGCGCACCGCTACGTCCGGTTCCTCCAGGCTCAGGTCGCCGCCCTCCAGGCCATGCCCATCGTCGCCTCCGACTCTGCCGGCTCCCTCCCCGTCCTGACACGCGGAGTCGGCGGCGGCACCGGTGAGGTGGAGATGCTGTCCAGGCTGAGCCGGAGCCAGATGCTGCAGGTGGCGGTGAACTCGCCGGTGGCGCAGACGAGGATGTCGGATCTCGGGAGGTGTGTGTTCTCGGTGGAGCAGCTGGCTTTGCTGAACAAGAAGAGCGTCATCAGGTCTGCCATGGTCTCTGCTTCAGGCCATTACTATCCTACTACTACTACTAGCTTCATTGACTCTTCTGCTTCTGCCTCTACCACTAATTTAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

306

Amino Acids

33.55

Weight (kDa)

8.69

Isoelectric Point (pI)

59.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 706
AasI GACNNNNNNGTC 1 cut(s) 751
AatII GACGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 706
AccB1I GGYRCC 4 cut(s) 245, 430, 489, 665
AccBSI CCGCTC 2 cut(s) 194, 214
AccII CGCG 2 cut(s) 454, 651
AclWI GGATC 2 cut(s) 51, 765
AcuI CTGAAG 1 cut(s) 825
AcvI CACGTG 1 cut(s) 437
AcyI GRCGYC 3 cut(s) 246, 263, 398
AfiI CCNNNNNNNGG 3 cut(s) 242, 304, 580
AgeI ACCGGT 1 cut(s) 668
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 4 cut(s) 447, 573, 594, 689
AluBI AGCT 5 cut(s) 388, 502, 790, 873, 917
AluI AGCT 5 cut(s) 388, 502, 790, 873, 917
Alw26I GTCTC 1 cut(s) 838
AlwI GGATC 2 cut(s) 51, 765
AlwNI CAGNNNCTG 3 cut(s) 472, 502, 712
Ama87I CYCGRG 2 cut(s) 542, 761
AoxI GGCC 3 cut(s) 81, 597, 844
ApeKI GCWGC 8 cut(s) 54, 57, 183, 444, 502, 505, 712, 787
AsiGI ACCGGT 1 cut(s) 668
AspLEI GCGC 5 cut(s) 248, 342, 454, 553, 742
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 4 cut(s) 82, 181, 683, 736
AvaI CYCGRG 2 cut(s) 542, 761
AvaII GGWCC 1 cut(s) 307
BanI GGYRCC 4 cut(s) 245, 430, 489, 665
BbrPI CACGTG 1 cut(s) 437
BbvI GCAGC 8 cut(s) 66, 69, 195, 456, 489, 492, 699, 799
BccI CCATC 4 cut(s) 128, 296, 517, 614
BceAI ACGGC 3 cut(s) 233, 259, 493
BciT130I CCWGG 4 cut(s) 449, 575, 596, 691
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 1 cut(s) 838
BfaI CTAG 2 cut(s) 368, 870
BfmI CTRYAG 3 cut(s) 181, 494, 713
BfoI RGCGCY 2 cut(s) 249, 343
BfuAI ACCTGC 1 cut(s) 706
BglI GCCNNNNNGGC 2 cut(s) 254, 596
BlpI GCTNAGC 2 cut(s) 473, 695
Bme1390I CCNGG 5 cut(s) 305, 449, 575, 596, 691
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 542, 761
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 8 cut(s) 247, 420, 432, 491, 569, 631, 667, 704
BmrFI CCNGG 5 cut(s) 305, 449, 575, 596, 691
BmsI GCATC 3 cut(s) 474, 672, 699
BpmI CTGGAG 2 cut(s) 557, 578
Bpu10I CCTNAGC 1 cut(s) 579
Bpu1102I GCTNAGC 2 cut(s) 473, 695
BpuMI CCSGG 1 cut(s) 305
BsaAI YACGTR 1 cut(s) 437
BsaHI GRCGYC 3 cut(s) 246, 263, 398
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 2 cut(s) 509, 828
BsaWI WCCGGW 3 cut(s) 421, 564, 668
BsaXI ACNNNNNCTCC 6 cut(s) 269, 299, 299, 329, 671, 701
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 304, 580
Bse118I RCCGGY 5 cut(s) 92, 415, 626, 668, 733
BseBI CCWGG 4 cut(s) 449, 575, 596, 691
BseDI CCNNGG 2 cut(s) 509, 828
BseGI GGATG 2 cut(s) 120, 756
BseLI CCNNNNNNNGG 3 cut(s) 242, 304, 580
BseMII CTCAG 6 cut(s) 92, 272, 425, 464, 593, 686
BseRI GAGGAG 4 cut(s) 302, 341, 364, 367
BseXI GCAGC 8 cut(s) 66, 69, 195, 456, 489, 492, 699, 799
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 454, 651
BshFI GGCC 3 cut(s) 83, 599, 846
BshNI GGYRCC 4 cut(s) 245, 430, 489, 665
BshTI ACCGGT 1 cut(s) 668
BsiHKCI CYCGRG 2 cut(s) 542, 761
BslFI GGGAC 1 cut(s) 253
BslI CCNNNNNNNGG 3 cut(s) 242, 304, 580
BsmAI GTCTC 1 cut(s) 838
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 3 cut(s) 83, 599, 846
Bso31I GGTCTC 1 cut(s) 838
BsoBI CYCGRG 2 cut(s) 542, 761
Bsp143I GATC 2 cut(s) 43, 757
Bsp1720I GCTNAGC 2 cut(s) 473, 695
Bsp19I CCATGG 1 cut(s) 828
BspANI GGCC 3 cut(s) 83, 599, 846
BspCNI CTCAG 6 cut(s) 91, 271, 424, 465, 592, 687
BspFNI CGCG 2 cut(s) 454, 651
BspLI GGNNCC 8 cut(s) 247, 420, 432, 491, 569, 631, 667, 704
BspMAI CTGCAG 3 cut(s) 185, 498, 717
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 2 cut(s) 51, 765
BspQI GCTCTTC 1 cut(s) 803
BspT107I GGYRCC 4 cut(s) 245, 430, 489, 665
BspTNI GGTCTC 1 cut(s) 838
BsrBI CCGCTC 2 cut(s) 194, 214
BsrFI RCCGGY 5 cut(s) 92, 415, 626, 668, 733
BssAI RCCGGY 5 cut(s) 92, 415, 626, 668, 733
BssECI CCNNGG 2 cut(s) 509, 828
BssMI GATC 2 cut(s) 43, 757
BssNI GRCGYC 3 cut(s) 246, 263, 398
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 4 cut(s) 449, 575, 596, 691
Bst6I CTCTTC 3 cut(s) 452, 803, 889
BstACI GRCGYC 3 cut(s) 246, 263, 398
BstAPI GCANNNNNTGC 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 4 cut(s) 417, 494, 628, 792
BstDEI CTNAG 6 cut(s) 78, 258, 411, 473, 579, 695
BstDSI CCRYGG 2 cut(s) 509, 828
BstF5I GGATG 2 cut(s) 120, 756
BstFNI CGCG 2 cut(s) 454, 651
BstH2I RGCGCY 2 cut(s) 249, 343
BstHHI GCGC 5 cut(s) 248, 342, 454, 553, 742
BstKTI GATC 2 cut(s) 46, 760
BstMAI GTCTC 1 cut(s) 838
BstMBI GATC 2 cut(s) 43, 757
BstNI CCWGG 4 cut(s) 449, 575, 596, 691
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 5 cut(s) 303, 447, 573, 594, 689
BstSFI CTRYAG 3 cut(s) 181, 494, 713
BstUI CGCG 2 cut(s) 454, 651
BstV1I GCAGC 8 cut(s) 66, 69, 195, 456, 489, 492, 699, 799
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BsuRI GGCC 3 cut(s) 83, 599, 846
BtgI CCRYGG 2 cut(s) 509, 828
BtsCI GGATG 2 cut(s) 120, 756
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 23
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 4 cut(s) 417, 494, 628, 792
CaiI CAGNNNCTG 3 cut(s) 472, 502, 712
CfoI GCGC 5 cut(s) 248, 342, 454, 553, 742
Cfr10I RCCGGY 5 cut(s) 92, 415, 626, 668, 733
Cfr13I GGNCC 1 cut(s) 307
CseI GACGC 3 cut(s) 406, 478, 802
CspAI ACCGGT 1 cut(s) 668
CviAII CATG 4 cut(s) 97, 538, 601, 829
DdeI CTNAG 6 cut(s) 78, 258, 411, 473, 579, 695
DinI GGCGCC 1 cut(s) 247
DpnI GATC 2 cut(s) 45, 759
DpnII GATC 2 cut(s) 43, 757
DrdI GACNNNNNNGTC 1 cut(s) 751
DseDI GACNNNNNNGTC 1 cut(s) 751
Eam1104I CTCTTC 3 cut(s) 452, 803, 889
EarI CTCTTC 3 cut(s) 452, 803, 889
Eco130I CCWWGG 1 cut(s) 828
Eco31I GGTCTC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 825
Eco72I CACGTG 1 cut(s) 437
Eco88I CYCGRG 2 cut(s) 542, 761
EcoRII CCWGG 4 cut(s) 447, 573, 594, 689
EcoT14I CCWWGG 1 cut(s) 828
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 4 cut(s) 100, 541, 604, 832
FaiI YATR 4 cut(s) 98, 539, 602, 830
FaqI GGGAC 1 cut(s) 253
FatI CATG 4 cut(s) 96, 537, 600, 828
FokI GGATG 2 cut(s) 107, 763
FspBI CTAG 2 cut(s) 368, 870
GlaI GCGC 5 cut(s) 247, 341, 453, 552, 741
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 2 cut(s) 557, 578
HaeII RGCGCY 2 cut(s) 249, 343
HaeIII GGCC 3 cut(s) 83, 599, 846
HgaI GACGC 3 cut(s) 406, 478, 802
HhaI GCGC 5 cut(s) 248, 342, 454, 553, 742
Hin1I GRCGYC 3 cut(s) 246, 263, 398
Hin1II CATG 4 cut(s) 100, 541, 604, 832
Hin6I GCGC 5 cut(s) 246, 340, 452, 551, 740
HinP1I GCGC 5 cut(s) 246, 340, 452, 551, 740
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 4 cut(s) 407, 620, 654, 881
HphI GGTGA 4 cut(s) 82, 181, 683, 736
Hpy166II GTNNAC 3 cut(s) 34, 109, 727
Hpy188I TCNGA 3 cut(s) 261, 619, 757
Hpy188III TCNNGA 3 cut(s) 47, 643, 763
Hpy8I GTNNAC 3 cut(s) 34, 109, 727
Hpy99I CGWCG 3 cut(s) 268, 400, 614
HpyAV CCTTC 2 cut(s) 34, 97
HpyCH4IV ACGT 4 cut(s) 36, 263, 436, 561
HpyCH4V TGCA 3 cut(s) 183, 496, 715
HpyF3I CTNAG 6 cut(s) 78, 258, 411, 473, 579, 695
HpySE526I ACGT 4 cut(s) 36, 263, 436, 561
Hsp92I GRCGYC 3 cut(s) 246, 263, 398
Hsp92II CATG 4 cut(s) 100, 541, 604, 832
HspAI GCGC 5 cut(s) 246, 340, 452, 551, 740
KasI GGCGCC 1 cut(s) 245
KroI GCCGGC 2 cut(s) 415, 626
KroNI GCCGGC 2 cut(s) 417, 628
Kzo9I GATC 2 cut(s) 43, 757
LguI GCTCTTC 1 cut(s) 803
LmnI GCTCC 5 cut(s) 199, 424, 635, 702, 784
Lsp1109I GCAGC 8 cut(s) 66, 69, 195, 456, 489, 492, 699, 799
LweI GCATC 3 cut(s) 474, 672, 699
MaeI CTAG 2 cut(s) 368, 870
MaeII ACGT 4 cut(s) 36, 263, 436, 561
MaeIII GTNAC 1 cut(s) 21
MalI GATC 2 cut(s) 45, 759
MbiI CCGCTC 2 cut(s) 194, 214
MboI GATC 2 cut(s) 43, 757
MboII GAAGA 6 cut(s) 283, 469, 529, 532, 820, 876
MflI RGATCY 1 cut(s) 757
MluCI AATT 1 cut(s) 907
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 614, 663, 875
MmeI TCCRAC 2 cut(s) 642, 735
MroNI GCCGGC 2 cut(s) 415, 626
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 790
MspR9I CCNGG 5 cut(s) 305, 449, 575, 596, 691
MvaI CCWGG 4 cut(s) 449, 575, 596, 691
MvnI CGCG 2 cut(s) 454, 651
NaeI GCCGGC 2 cut(s) 417, 628
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 305
NcoI CCATGG 1 cut(s) 828
NdeII GATC 2 cut(s) 43, 757
NgoMIV GCCGGC 2 cut(s) 415, 626
NlaIII CATG 4 cut(s) 100, 541, 604, 832
NlaIV GGNNCC 8 cut(s) 247, 420, 432, 491, 569, 631, 667, 704
NmuCI GTSAC 1 cut(s) 21
NspI RCATGY 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 542
PaqCI CACCTGC 1 cut(s) 706
PciSI GCTCTTC 1 cut(s) 803
PcsI WCGNNNNNNNCGW 2 cut(s) 260, 615
PdiI GCCGGC 2 cut(s) 417, 628
PfeI GAWTC 1 cut(s) 407
PflFI GACNNNGTC 1 cut(s) 265
PfoI TCCNGGA 1 cut(s) 303
PinAI ACCGGT 1 cut(s) 668
PleI GAGTC 3 cut(s) 614, 662, 875
PluTI GGCGCC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 437
PmlI CACGTG 1 cut(s) 437
PpsI GAGTC 3 cut(s) 614, 662, 875
Ppu21I YACGTR 1 cut(s) 437
Psp6I CCWGG 4 cut(s) 447, 573, 594, 689
PspCI CACGTG 1 cut(s) 437
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 4 cut(s) 447, 573, 594, 689
PspN4I GGNNCC 8 cut(s) 247, 420, 432, 491, 569, 631, 667, 704
PspPI GGNCC 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 542
PstI CTGCAG 3 cut(s) 185, 498, 717
PstNI CAGNNNCTG 3 cut(s) 472, 502, 712
PsuI RGATCY 1 cut(s) 757
PsyI GACNNNGTC 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 790
RseI CAYNNNNRTG 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 803
Sau3AI GATC 2 cut(s) 43, 757
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 3 cut(s) 614, 663, 875
ScrFI CCNGG 5 cut(s) 305, 449, 575, 596, 691
SfaNI GCATC 3 cut(s) 474, 672, 699
SfcI CTRYAG 3 cut(s) 181, 494, 713
SfoI GGCGCC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 542
SgrAI CRCCGGYG 2 cut(s) 668, 733
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 1 cut(s) 907
SspDI GGCGCC 1 cut(s) 245
SspMI CTAG 2 cut(s) 368, 870
StyD4I CCNGG 5 cut(s) 303, 447, 573, 594, 689
StyI CCWWGG 1 cut(s) 828
TaiI ACGT 4 cut(s) 39, 266, 439, 564
TaqI TCGA 3 cut(s) 321, 354, 543
TasI AATT 1 cut(s) 907
TauI GCSGC 8 cut(s) 131, 214, 403, 466, 551, 590, 663, 666
TfiI GAWTC 1 cut(s) 407
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 8 cut(s) 54, 57, 183, 444, 502, 505, 712, 787
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 1 cut(s) 865
TspRI CASTG 1 cut(s) 30
Tth111I GACNNNGTC 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 307
XceI RCATGY 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 542
XspI CTAG 2 cut(s) 368, 870
ZraI GACGTC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.