Rh2BG619700

3-dehydroquinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008793
Physical Location & Seq
Reverse (-)
84240801 .. 84243897
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG619700.1

Sequence Viewer

Length: 1053 bp
ATGGCTCAGTCTCTGCTGCACTCTGCCTCTTTGGCCGGAATCCGCTGCTTCACTCCCCCCTCAGATAAATGGAGTAACATTTGCAGATTGATTTCTTCACATAATAAACACCCAATGGAAACCAAGGCCACCCAGAGTTCATCAGTTGCTTCTTCTACGACGTCGTTTGGGAGCTCGAACAAGACGGTGTGGGTTTGGACGGAGAGCAAGCAGGTCATGACGGCCGCCGTGGAGAGAGGCTGGAACACCTTCGTGTTTCAGAGTCAGAAACTCGCCGATGACTGGTCTTCAATTGCGTTGATAGATCCTCTTTTGATCAAAGAGGGAGGGATTTTCGATAGCAAGAACTCGAGAGTTGCTACAGTTGTTGAGGTTTCCAATCCCAAAGAGTTGGAAGAGCTTCAACCCGAAAACGGGGTGGCTGAGAATGTTGTTGTTGATTTGCTAGATTGGCAGGTGATACCGGCGGAGAATATAGTTGCAGCATTTCAAGGGAGTCAGAAAACAGTGTTTGCCGTTTCGAAAACCCCTGTCGAAGCACAAGTCTTCTTTGAGGCTCTCGAGCATGGTCTGGGTGGAGTTGTTTTGAAAGTTGAGGATGTTCAAGCAGTTCTTGACCTAAAGGACTATTTTGACAGAAGGGACGAAGTGGGCAACATATTGAGTTTGACCAAAGCCATTGTTACTGGAGTCCAAGTAGCTGGAATGGGGGATCGGGTCTGTGTTGATCTCTGTAGTCTCATGAGACCTGGTGAAGGACTGCTTGTTGGATCATTTGCCAGAGGACTATTTCTGGTTCATTCAGAATGCTTGGAGTCAAATTACATTGCCAGCAGACCTTTCCGTGTCAATGCAGGACCTGTACATGCCTATGTTGCTGTTCCAGGAGGAAAAACAAGCTACCTCTCAGAGTTGAAAGCAGGAAAAGAGGTGATTCTGGTTGATCAGAAAGGTCATCAACGAACAGCAATTGTTGGACGTGTAAAGATAGAGACGAGACCACTAATCCTTGTGGAGGCAAAGGTTTGTACTAAATACAATAACATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHQS

Protein Analysis

350

Amino Acids

38.14

Weight (kDa)

6.2

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHQS PF01959 60 - 211 6.8e-45 3-dehydroquinate synthase II N-terminal domain
DHQS_2nd PF26558 226 - 344 3.2e-58 3-dehydroquinate synthase II C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
AatII GACGTC 1 cut(s) 164
Acc36I ACCTGC 2 cut(s) 202, 445
AciI CCGC 3 cut(s) 43, 225, 467
AclWI GGATC 3 cut(s) 299, 720, 778
AcoI YGGCCR 2 cut(s) 33, 222
AcyI GRCGYC 1 cut(s) 161
AfaI GTAC 2 cut(s) 864, 1030
AfiI CCNNNNNNNGG 5 cut(s) 282, 413, 414, 755, 1015
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 6 cut(s) 291, 404, 491, 589, 605, 916
AjiI CACGTC 1 cut(s) 980
AjnI CCWGG 2 cut(s) 748, 883
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 4 cut(s) 174, 400, 701, 900
AluI AGCT 4 cut(s) 174, 400, 701, 900
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 5 cut(s) 15, 739, 743, 986, 991
AlwI GGATC 3 cut(s) 299, 720, 778
AlwNI CAGNNNCTG 2 cut(s) 13, 860
Ama87I CYCGRG 2 cut(s) 349, 560
AoxI GGCC 3 cut(s) 33, 126, 222
ApeKI GCWGC 3 cut(s) 16, 45, 482
ArsI GACNNNNNNTTYG 2 cut(s) 528, 560
Asp700I GAANNNNTTC 2 cut(s) 248, 399
AspS9I GGNCC 1 cut(s) 857
AsuHPI GGTGA 3 cut(s) 469, 764, 943
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 2 cut(s) 349, 560
AvaII GGWCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 176
BbsI GAAGAC 2 cut(s) 279, 538
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 3 cut(s) 3, 32, 494
BceAI ACGGC 3 cut(s) 212, 237, 500
BciT130I CCWGG 2 cut(s) 750, 885
BclI TGATCA 2 cut(s) 315, 943
BcoDI GTCTC 5 cut(s) 15, 739, 743, 986, 991
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 360, 733
BfuAI ACCTGC 2 cut(s) 202, 445
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 4 cut(s) 17, 46, 225, 483
BlsI GCNGC 4 cut(s) 18, 47, 226, 484
Bme1390I CCNGG 2 cut(s) 750, 885
Bme18I GGWCC 1 cut(s) 857
BmeT110I CYCGRG 2 cut(s) 349, 560
BmgBI CACGTC 1 cut(s) 980
BmgT120I GGNCC 1 cut(s) 857
BmrFI CCNGG 2 cut(s) 750, 885
BpiI GAAGAC 2 cut(s) 279, 538
BpmI CTGGAG 1 cut(s) 708
Bpu14I TTCGAA 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 161
BsaI GGTCTC 2 cut(s) 739, 991
BsaJI CCNNGG 2 cut(s) 123, 228
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 413, 414, 755, 1015
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 2 cut(s) 287, 691
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 2 cut(s) 750, 885
BseDI CCNNGG 2 cut(s) 123, 228
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 5 cut(s) 282, 413, 414, 755, 1015
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 4 cut(s) 20, 75, 414, 921
BseNI ACTGG 2 cut(s) 287, 691
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 3 cut(s) 3, 32, 494
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 35, 128, 224
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 2 cut(s) 349, 560
BsiSI CCGG 2 cut(s) 36, 464
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 5 cut(s) 282, 413, 414, 755, 1015
BsmAI GTCTC 5 cut(s) 15, 739, 743, 986, 991
BsmBI CGTCTC 1 cut(s) 986
BsmFI GGGAC 1 cut(s) 656
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 3 cut(s) 35, 128, 224
Bso31I GGTCTC 2 cut(s) 739, 991
BsoBI CYCGRG 2 cut(s) 349, 560
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 862
Bsp143I GATC 6 cut(s) 304, 315, 712, 727, 770, 943
BspACI CCGC 3 cut(s) 43, 225, 467
BspANI GGCC 3 cut(s) 35, 128, 224
BspCNI CTCAG 4 cut(s) 19, 74, 415, 920
BspHI TCATGA 2 cut(s) 216, 741
BspMI ACCTGC 2 cut(s) 202, 445
BspPI GGATC 3 cut(s) 299, 720, 778
BspQI GCTCTTC 1 cut(s) 390
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 739, 991
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 463
BsrGI TGTACA 1 cut(s) 862
BsrI ACTGG 2 cut(s) 287, 691
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 123, 228
BssMI GATC 6 cut(s) 304, 315, 712, 727, 770, 943
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 750, 885
Bst4CI ACNGT 3 cut(s) 187, 364, 508
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 862
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 209, 832
BstDEI CTNAG 4 cut(s) 6, 61, 423, 907
BstDSI CCRYGG 1 cut(s) 228
BstENI CCTNNNNNAGG 2 cut(s) 753, 1013
BstF5I GGATG 1 cut(s) 604
BstKTI GATC 6 cut(s) 307, 318, 715, 730, 773, 946
BstMAI GTCTC 5 cut(s) 15, 739, 743, 986, 991
BstMBI GATC 6 cut(s) 304, 315, 712, 727, 770, 943
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 3 cut(s) 32, 451, 875
BstNI CCWGG 2 cut(s) 750, 885
BstNSI RCATGY 1 cut(s) 869
BstSCI CCNGG 2 cut(s) 748, 883
BstSFI CTRYAG 2 cut(s) 360, 733
BstV1I GCAGC 3 cut(s) 3, 32, 494
BstV2I GAAGAC 2 cut(s) 279, 538
BstX2I RGATCY 1 cut(s) 304
BstXI CCANNNNNNTGG 2 cut(s) 391, 701
BstYI RGATCY 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 35, 128, 224
BtgI CCRYGG 1 cut(s) 228
BtrI CACGTC 1 cut(s) 980
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 2 cut(s) 202, 445
Cac8I GCNNGC 2 cut(s) 209, 832
CaiI CAGNNNCTG 2 cut(s) 13, 860
CciI TCATGA 2 cut(s) 216, 741
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 857
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 2 cut(s) 863, 1029
CviAII CATG 5 cut(s) 217, 566, 742, 866, 1045
CviQI GTAC 2 cut(s) 863, 1029
DdeI CTNAG 4 cut(s) 6, 61, 423, 907
DpnI GATC 6 cut(s) 306, 317, 714, 729, 772, 945
DpnII GATC 6 cut(s) 304, 315, 712, 727, 770, 943
EaeI YGGCCR 2 cut(s) 33, 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 176
Eco31I GGTCTC 2 cut(s) 739, 991
Eco47I GGWCC 1 cut(s) 857
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 174
Eco88I CYCGRG 2 cut(s) 349, 560
EcoICRI GAGCTC 1 cut(s) 174
EcoNI CCTNNNNNAGG 2 cut(s) 753, 1013
EcoO109I RGGNCCY 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 748, 883
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 986
FaeI CATG 5 cut(s) 220, 569, 745, 869, 1048
FaiI YATR 9 cut(s) 102, 218, 476, 567, 659, 743, 867, 873, 1046
FalI AAGNNNNNCTT 4 cut(s) 597, 629, 747, 779
FaqI GGGAC 1 cut(s) 656
FatI CATG 5 cut(s) 216, 565, 741, 865, 1044
FbaI TGATCA 2 cut(s) 315, 943
Fnu4HI GCNGC 4 cut(s) 17, 46, 225, 483
FokI GGATG 1 cut(s) 611
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 4 cut(s) 17, 46, 225, 483
FspBI CTAG 1 cut(s) 446
GluI GCNGC 4 cut(s) 17, 46, 225, 483
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 3 cut(s) 35, 128, 224
HapII CCGG 2 cut(s) 36, 464
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 5 cut(s) 220, 569, 745, 869, 1048
HinfI GANTC 6 cut(s) 39, 262, 496, 690, 815, 934
HpaII CCGG 2 cut(s) 36, 464
HphI GGTGA 3 cut(s) 469, 764, 943
Hpy188I TCNGA 7 cut(s) 64, 261, 267, 501, 805, 910, 948
Hpy188III TCNNGA 5 cut(s) 217, 351, 560, 614, 742
Hpy99I CGWCG 2 cut(s) 163, 166
HpyAV CCTTC 3 cut(s) 259, 633, 749
HpyCH4III ACNGT 3 cut(s) 187, 364, 508
HpyCH4IV ACGT 2 cut(s) 161, 979
HpyCH4V TGCA 4 cut(s) 19, 84, 482, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 451, 875
HpyF3I CTNAG 4 cut(s) 6, 61, 423, 907
HpySE526I ACGT 2 cut(s) 161, 979
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 5 cut(s) 220, 569, 745, 869, 1048
Ksp22I TGATCA 2 cut(s) 315, 943
Kzo9I GATC 6 cut(s) 304, 315, 712, 727, 770, 943
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 171
Lsp1109I GCAGC 3 cut(s) 3, 32, 494
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 1 cut(s) 446
MaeII ACGT 2 cut(s) 161, 979
MaeIII GTNAC 2 cut(s) 74, 682
MalI GATC 6 cut(s) 306, 317, 714, 729, 772, 945
MboI GATC 6 cut(s) 304, 315, 712, 727, 770, 943
MboII GAAGA 5 cut(s) 87, 144, 279, 407, 538
MfeI CAATTG 2 cut(s) 291, 969
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 3 cut(s) 291, 820, 969
MlyI GAGTC 4 cut(s) 271, 505, 699, 824
MmeI TCCRAC 3 cut(s) 372, 748, 955
MroXI GAANNNNTTC 2 cut(s) 248, 399
MslI CAYNNNNRTG 2 cut(s) 251, 870
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 2 cut(s) 36, 464
MspR9I CCNGG 2 cut(s) 750, 885
MunI CAATTG 2 cut(s) 291, 969
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 2 cut(s) 750, 885
MwoI GCNNNNNNNGC 3 cut(s) 32, 451, 875
NdeII GATC 6 cut(s) 304, 315, 712, 727, 770, 943
NlaIII CATG 5 cut(s) 220, 569, 745, 869, 1048
NspI RCATGY 1 cut(s) 869
NspV TTCGAA 1 cut(s) 521
OliI CACNNNNGTG 1 cut(s) 251
PaeR7I CTCGAG 2 cut(s) 349, 560
PagI TCATGA 2 cut(s) 216, 741
PaqCI CACCTGC 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 390
PctI GAATGC 1 cut(s) 812
PdmI GAANNNNTTC 2 cut(s) 248, 399
PfeI GAWTC 2 cut(s) 39, 934
PfoI TCCNGGA 1 cut(s) 883
PkrI GCNGC 4 cut(s) 18, 47, 226, 484
PleI GAGTC 4 cut(s) 270, 504, 698, 823
PpsI GAGTC 4 cut(s) 270, 504, 698, 823
PpuMI RGGWCCY 1 cut(s) 857
Psp124BI GAGCTC 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 748, 883
PspGI CCWGG 2 cut(s) 748, 883
PspPI GGNCC 1 cut(s) 857
PspPPI RGGWCCY 1 cut(s) 857
PstNI CAGNNNCTG 2 cut(s) 13, 860
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 2 cut(s) 864, 1030
RsaNI GTAC 2 cut(s) 863, 1029
RseI CAYNNNNRTG 2 cut(s) 251, 870
SacI GAGCTC 1 cut(s) 176
SapI GCTCTTC 1 cut(s) 390
SatI GCNGC 4 cut(s) 17, 46, 225, 483
Sau3AI GATC 6 cut(s) 304, 315, 712, 727, 770, 943
Sau96I GGNCC 1 cut(s) 857
SchI GAGTC 4 cut(s) 271, 505, 699, 824
ScrFI CCNGG 2 cut(s) 750, 885
SduI GDGCHC 1 cut(s) 176
SexAI ACCWGGT 1 cut(s) 748
SfcI CTRYAG 2 cut(s) 360, 733
Sfr274I CTCGAG 2 cut(s) 349, 560
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 857
SlaI CTCGAG 2 cut(s) 349, 560
SmiMI CAYNNNNRTG 2 cut(s) 251, 870
SmlI CTYRAG 2 cut(s) 349, 560
SmoI CTYRAG 2 cut(s) 349, 560
Sse9I AATT 3 cut(s) 291, 820, 969
SsiI CCGC 3 cut(s) 43, 225, 467
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 748, 883
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 187, 364, 508
TaiI ACGT 2 cut(s) 164, 982
TaqI TCGA 6 cut(s) 176, 336, 350, 521, 534, 561
TasI AATT 3 cut(s) 291, 820, 969
TatI WGTACW 2 cut(s) 862, 1028
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 2 cut(s) 39, 934
TscAI CASTG 1 cut(s) 513
TseI GCWGC 3 cut(s) 16, 45, 482
TspDTI ATGAA 2 cut(s) 129, 788
TspGWI ACGGA 2 cut(s) 215, 833
TspRI CASTG 1 cut(s) 513
VpaK11BI GGWCC 1 cut(s) 857
XagI CCTNNNNNAGG 2 cut(s) 753, 1013
XceI RCATGY 1 cut(s) 869
XhoI CTCGAG 2 cut(s) 349, 560
XmnI GAANNNNTTC 2 cut(s) 248, 399
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.