Rorug03G0100000

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014364
Physical Location & Seq
Reverse (-)
7844788 .. 7847519
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0100000.1

Sequence Viewer

Length: 351 bp
ATGGCATCTGCAGTGGTGAAGCTTGCTTTGGTGGCTCTCATGTGCATTGTGGTGGCTGTCCCCGTCGCCCAGGCCATCACCTGCGGCCAAGTGACCCAAAACGTGGCACCGTGCATCAACTACGTCAAGAGTGGTGGCCCTGTCCCTGCCGCTTGCTGCAACGGAGTCCGCAACCTTAACAGCCAGGCGAAGACCACAGCCGACCGCAAGCAAACTTGTACTTGCCTAAAGAACGCAGCTGGATCCATTCCCGGAGTTAACCCTAACCTCGCCGCTGGTCTTCCCGGCAAATGTGGTGTCAGCGTTCCCTACAAGATCAGCACGTCCACCAACTGCAACAATGTGAAGTGA

Protein Analysis

116

Amino Acids

11.67

Weight (kDa)

9.34

Isoelectric Point (pI)

26.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 17 - 105 1.5e-06 Probable lipid transfer
Tryp_alpha_amyl PF00234 28 - 112 4.5e-15 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37130 AT2G37130
fragaria_vesca FvH4_6g15580
malus_domestica MD04G1101700.v1.1
prunus_persica Prupe.6G239400_v2.0.a1 Prupe.6G239400_v2.0.a1
pyrus_communis pycom04g09720
rosa_chinensis RchiOBHm_Chr3g0469141
rosa_laevigata RLG00000024370
rosa_multiflora Rmu_sc0000302.1_g000012
rosa_roxburghii Rroxscaffold_6G00412160
rosa_rugosa Rorug03G0100000 Rorug03G0100000
rosa_samantha Rh3AG149100 Rh3BG172300 Rh3DG203300
rosa_wichuraiana Rw3G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 5 cut(s) 84, 150, 169, 205, 273
AclWI GGATC 2 cut(s) 237, 250
AcoI YGGCCR 1 cut(s) 85
AfaI GTAC 1 cut(s) 220
AfiI CCNNNNNNNGG 1 cut(s) 103
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 2 cut(s) 69, 183
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 2 cut(s) 22, 239
AluI AGCT 2 cut(s) 22, 239
AlwI GGATC 2 cut(s) 237, 250
AoxI GGCC 3 cut(s) 72, 85, 136
ApeKI GCWGC 2 cut(s) 156, 236
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspS9I GGNCC 1 cut(s) 137
AsuC2I CCSGG 2 cut(s) 252, 285
AsuHPI GGTGA 2 cut(s) 28, 70
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 106
BbsI GAAGAC 2 cut(s) 197, 272
BbvI GCAGC 2 cut(s) 143, 248
BccI CCATC 1 cut(s) 83
BciT130I CCWGG 2 cut(s) 71, 185
BcnI CCSGG 2 cut(s) 252, 285
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 89
BisI GCNGC 5 cut(s) 85, 150, 157, 237, 273
BlsI GCNGC 5 cut(s) 86, 151, 158, 238, 274
Bme1390I CCNGG 4 cut(s) 71, 185, 252, 285
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 2 cut(s) 108, 244
BmrFI CCNGG 4 cut(s) 71, 185, 252, 285
BmsI GCATC 2 cut(s) 14, 123
BpiI GAAGAC 2 cut(s) 197, 272
BpuMI CCSGG 2 cut(s) 252, 285
BsaJI CCNNGG 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 103
BseBI CCWGG 2 cut(s) 71, 185
BseDI CCNNGG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 103
BseXI GCAGC 2 cut(s) 143, 248
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 74, 87, 138
BshNI GGYRCC 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 252, 285
BslFI GGGAC 2 cut(s) 44, 128
BslI CCNNNNNNNGG 1 cut(s) 103
BsmFI GGGAC 2 cut(s) 44, 128
BsnI GGCC 3 cut(s) 74, 87, 138
Bsp143I GATC 2 cut(s) 242, 315
BspACI CCGC 5 cut(s) 84, 150, 169, 205, 273
BspANI GGCC 3 cut(s) 74, 87, 138
BspLI GGNNCC 2 cut(s) 108, 244
BspMAI CTGCAG 1 cut(s) 13
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 2 cut(s) 237, 250
BspT107I GGYRCC 1 cut(s) 106
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 2 cut(s) 242, 315
Bst2UI CCWGG 2 cut(s) 71, 185
Bst4CI ACNGT 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 24, 154, 209
BstKTI GATC 2 cut(s) 245, 318
BstMBI GATC 2 cut(s) 242, 315
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNI CCWGG 2 cut(s) 71, 185
BstSCI CCNGG 4 cut(s) 69, 183, 250, 283
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 143, 248
BstV2I GAAGAC 2 cut(s) 197, 272
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 3 cut(s) 74, 87, 138
BtrI CACGTC 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 3 cut(s) 24, 154, 209
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 219
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 1 cut(s) 40
CviJI RGCY 9 cut(s) 22, 35, 56, 74, 87, 138, 183, 200, 239
CviKI_1 RGCY 9 cut(s) 22, 35, 56, 74, 87, 138, 183, 200, 239
CviQI GTAC 1 cut(s) 219
DpnI GATC 2 cut(s) 244, 317
DpnII GATC 2 cut(s) 242, 315
EaeI YGGCCR 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 69, 183
FaeI CATG 1 cut(s) 43
FaiI YATR 1 cut(s) 41
FaqI GGGAC 2 cut(s) 44, 128
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 5 cut(s) 85, 150, 157, 237, 273
Fsp4HI GCNGC 5 cut(s) 85, 150, 157, 237, 273
GluI GCNGC 5 cut(s) 85, 150, 157, 237, 273
HaeIII GGCC 3 cut(s) 74, 87, 138
HapII CCGG 2 cut(s) 252, 285
Hin1II CATG 1 cut(s) 43
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 1 cut(s) 165
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 2 cut(s) 252, 285
HphI GGTGA 2 cut(s) 28, 70
Hpy166II GTNNAC 2 cut(s) 259, 327
Hpy188III TCNNGA 1 cut(s) 127
Hpy8I GTNNAC 2 cut(s) 259, 327
Hpy99I CGWCG 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 3 cut(s) 102, 123, 323
HpyCH4V TGCA 5 cut(s) 11, 45, 114, 159, 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpySE526I ACGT 3 cut(s) 102, 123, 323
Hsp92II CATG 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 242, 315
Lsp1109I GCAGC 2 cut(s) 143, 248
LweI GCATC 2 cut(s) 14, 123
MaeII ACGT 3 cut(s) 102, 123, 323
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 244, 317
MboI GATC 2 cut(s) 242, 315
MboII GAAGA 2 cut(s) 202, 272
MflI RGATCY 1 cut(s) 242
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 1 cut(s) 278
MseI TTAA 2 cut(s) 177, 258
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 2 cut(s) 239, 275
MspI CCGG 2 cut(s) 252, 285
MspR9I CCNGG 4 cut(s) 71, 185, 252, 285
MvaI CCWGG 2 cut(s) 71, 185
MwoI GCNNNNNNNGC 1 cut(s) 32
NciI CCSGG 2 cut(s) 252, 285
NdeII GATC 2 cut(s) 242, 315
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 2 cut(s) 108, 244
NmuCI GTSAC 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 89
PflMI CCANNNNNTGG 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 5 cut(s) 86, 151, 158, 238, 274
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 69, 183
PspGI CCWGG 2 cut(s) 69, 183
PspN4I GGNNCC 2 cut(s) 108, 244
PspPI GGNCC 1 cut(s) 137
PstI CTGCAG 1 cut(s) 13
PsuI RGATCY 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 177, 258
SatI GCNGC 5 cut(s) 85, 150, 157, 237, 273
Sau3AI GATC 2 cut(s) 242, 315
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 4 cut(s) 71, 185, 252, 285
SetI ASST 8 cut(s) 24, 83, 105, 126, 177, 241, 270, 326
SfaNI GCATC 2 cut(s) 14, 123
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 50
SsiI CCGC 5 cut(s) 84, 150, 169, 205, 273
StyD4I CCNGG 4 cut(s) 69, 183, 250, 283
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 3 cut(s) 105, 126, 326
TatI WGTACW 1 cut(s) 218
TauI GCSGC 3 cut(s) 87, 152, 275
Tru1I TTAA 2 cut(s) 177, 258
Tru9I TTAA 2 cut(s) 177, 258
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 2 cut(s) 156, 236
Tsp45I GTSAC 1 cut(s) 91
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.