Rorug03G0106500

DVL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0016494
Physical Location & Seq
Reverse (-)
8522038 .. 8523806
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0106500.1

Sequence Viewer

Length: 639 bp
ATGACGACGAATCAAGACGACGCATCGCAGAATCATGACCAGCAACAACAAAAACAATCCCCGCCGGTTGTCCCCACGGCTCCACCGGCTGCTTCTGCTGACGTGGAGAGCCAGACGGAGCCGGCACCGGCCCAGGCCCGGGCGGCTGGGAGCGGGTTTGGGGTGGCAGGGATCATACGGCGGTGGAAGAGAGATGACTTGTTGAGGAAAGGCTCTCTGGCATTGAGAGGACTGTCCTTGGTCTTCTCTTTGCTGGCTTTCATCATCATGGCCAGTAACAAACACGGCGATTGGAAAGACTTCGATAAATATGAAGAATACAGGTATTTGTTGGGAATAGCGATATTGGCGACGTTGTACACCGGCGCACAAGCTTTCAAAAATGCCACCGGGAAAGAGATGTTTCAGCGCCGGACGTCGGCGTTGGCCGACTTCATCGGAGATCAGATTATGGCATACCTTCTGATATCATCATCATCTTCGGCGATTCCGTTAACAAATAGGATGAGGGAATCACAAGACAACATCTTTACAGACGCCTCTGCATCTGCCATTAGTATGGCTTTTCTCGCTTTCGTTTCCCTTGCACTCTTGGCCCTCATTTCCGGACACAAGTTATCTTCTCAGTCTTACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DVL

Protein Analysis

212

Amino Acids

23.02

Weight (kDa)

7.95

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 67 - 193 1e-26 Casparian strip membrane protein domain
MARVEL PF01284 69 - 198 1.6e-08 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 124
AccBSI CCGCTC 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 605
AciI CCGC 4 cut(s) 62, 143, 153, 181
AclWI GGATC 1 cut(s) 179
AcoI YGGCCR 2 cut(s) 270, 426
AcyI GRCGYC 2 cut(s) 416, 537
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 3 cut(s) 138, 139, 418
AgsI TTSAA 1 cut(s) 379
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
AlwI GGATC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 138
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 5 cut(s) 129, 135, 270, 426, 594
ApeKI GCWGC 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 299
AspLEI GCGC 2 cut(s) 368, 411
AspS9I GGNCC 3 cut(s) 130, 136, 595
AsuC2I CCSGG 3 cut(s) 139, 140, 391
AvaI CYCGRG 1 cut(s) 138
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 76
BceAI ACGGC 3 cut(s) 93, 194, 301
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 3 cut(s) 139, 140, 391
BfoI RGCGCY 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 2 cut(s) 90, 144
BlsI GCNGC 2 cut(s) 91, 145
Bme1390I CCNGG 4 cut(s) 134, 139, 140, 391
BmeT110I CYCGRG 1 cut(s) 138
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 130, 136, 595
BmiI GGNNCC 3 cut(s) 81, 120, 126
BmrFI CCNGG 4 cut(s) 134, 139, 140, 391
BmsI GCATC 2 cut(s) 32, 554
BpiI GAAGAC 1 cut(s) 235
BpuMI CCSGG 3 cut(s) 139, 140, 391
BsaHI GRCGYC 2 cut(s) 416, 537
BsaJI CCNNGG 4 cut(s) 75, 132, 138, 237
BsaWI WCCGGW 1 cut(s) 605
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 139, 418
Bse118I RCCGGY 5 cut(s) 64, 85, 121, 127, 362
Bse1I ACTGG 1 cut(s) 273
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 4 cut(s) 75, 132, 138, 237
BseGI GGATG 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 138, 139, 418
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 146
BshFI GGCC 5 cut(s) 131, 137, 272, 428, 596
BshNI GGYRCC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 9 cut(s) 65, 86, 122, 128, 139, 363, 390, 412, 606
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 3 cut(s) 138, 139, 418
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 5 cut(s) 131, 137, 272, 428, 596
BsoBI CYCGRG 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 605
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 2 cut(s) 171, 442
BspACI CCGC 4 cut(s) 62, 143, 153, 181
BspANI GGCC 5 cut(s) 131, 137, 272, 428, 596
BspCNI CTCAG 1 cut(s) 637
BspEI TCCGGA 1 cut(s) 605
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 3 cut(s) 81, 120, 126
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 153
BsrFI RCCGGY 5 cut(s) 64, 85, 121, 127, 362
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 1 cut(s) 273
BssAI RCCGGY 5 cut(s) 64, 85, 121, 127, 362
BssECI CCNNGG 4 cut(s) 75, 132, 138, 237
BssMI GATC 2 cut(s) 171, 442
BssNI GRCGYC 2 cut(s) 416, 537
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 234
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 416, 537
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 123, 255
BstDEI CTNAG 1 cut(s) 624
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 510
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 2 cut(s) 368, 411
BstKTI GATC 2 cut(s) 174, 445
BstMBI GATC 2 cut(s) 171, 442
BstMWI GCNNNNNNNGC 6 cut(s) 86, 95, 143, 347, 569, 593
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 132, 137, 138, 389
BstV1I GCAGC 1 cut(s) 76
BstV2I GAAGAC 1 cut(s) 235
BstXI CCANNNNNNTGG 1 cut(s) 559
BsuRI GGCC 5 cut(s) 131, 137, 272, 428, 596
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 123, 255
CciI TCATGA 1 cut(s) 34
CfoI GCGC 2 cut(s) 368, 411
Cfr10I RCCGGY 5 cut(s) 64, 85, 121, 127, 362
Cfr13I GGNCC 3 cut(s) 130, 136, 595
Cfr9I CCCGGG 1 cut(s) 138
CseI GACGC 2 cut(s) 29, 545
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 35, 268
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 1 cut(s) 624
DpnI GATC 2 cut(s) 173, 444
DpnII GATC 2 cut(s) 171, 442
EaeI YGGCCR 2 cut(s) 270, 426
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 237
Eco32I GATATC 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 2 cut(s) 38, 271
FaiI YATR 7 cut(s) 36, 176, 269, 312, 452, 457, 560
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 34, 267
FauI CCCGC 2 cut(s) 69, 146
Fnu4HI GCNGC 2 cut(s) 90, 144
FokI GGATG 1 cut(s) 517
Fsp4HI GCNGC 2 cut(s) 90, 144
GlaI GCGC 2 cut(s) 367, 410
GluI GCNGC 2 cut(s) 90, 144
GsaI CCCAGC 1 cut(s) 150
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 5 cut(s) 131, 137, 272, 428, 596
HapII CCGG 9 cut(s) 65, 86, 122, 128, 139, 363, 390, 412, 606
HgaI GACGC 2 cut(s) 29, 545
HhaI GCGC 2 cut(s) 368, 411
Hin1I GRCGYC 2 cut(s) 416, 537
Hin1II CATG 2 cut(s) 38, 271
Hin6I GCGC 2 cut(s) 366, 409
HinP1I GCGC 2 cut(s) 366, 409
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 4 cut(s) 10, 31, 487, 512
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 9 cut(s) 65, 86, 122, 128, 139, 363, 390, 412, 606
Hpy166II GTNNAC 2 cut(s) 360, 495
Hpy188I TCNGA 4 cut(s) 440, 447, 465, 638
Hpy188III TCNNGA 3 cut(s) 14, 35, 606
Hpy8I GTNNAC 2 cut(s) 360, 495
Hpy99I CGWCG 4 cut(s) 10, 23, 355, 421
HpyAV CCTTC 1 cut(s) 470
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4IV ACGT 3 cut(s) 102, 353, 416
HpyCH4V TGCA 2 cut(s) 545, 587
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 95, 143, 347, 569, 593
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 3 cut(s) 102, 353, 416
Hsp92I GRCGYC 2 cut(s) 416, 537
Hsp92II CATG 2 cut(s) 38, 271
HspAI GCGC 2 cut(s) 366, 409
Kpn2I TCCGGA 1 cut(s) 605
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 2 cut(s) 171, 442
LmnI GCTCC 3 cut(s) 85, 118, 150
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 2 cut(s) 32, 554
MaeII ACGT 3 cut(s) 102, 353, 416
MaeIII GTNAC 1 cut(s) 275
MalI GATC 2 cut(s) 173, 444
MbiI CCGCTC 1 cut(s) 153
MboI GATC 2 cut(s) 171, 442
MboII GAAGA 5 cut(s) 199, 235, 326, 471, 612
MlsI TGGCCA 1 cut(s) 272
MluNI TGGCCA 1 cut(s) 272
MnlI CCTC 5 cut(s) 198, 221, 501, 550, 608
Mox20I TGGCCA 1 cut(s) 272
MroI TCCGGA 1 cut(s) 605
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 272
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 2 cut(s) 266, 557
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 9 cut(s) 65, 86, 122, 128, 139, 363, 390, 412, 606
MspR9I CCNGG 4 cut(s) 134, 139, 140, 391
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 6 cut(s) 86, 95, 143, 347, 569, 593
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 3 cut(s) 139, 140, 391
NdeII GATC 2 cut(s) 171, 442
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 38, 271
NlaIV GGNNCC 3 cut(s) 81, 120, 126
PagI TCATGA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 4 cut(s) 10, 31, 487, 512
PkrI GCNGC 2 cut(s) 91, 145
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 3 cut(s) 81, 120, 126
PspPI GGNCC 3 cut(s) 130, 136, 595
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 2 cut(s) 266, 557
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 2 cut(s) 90, 144
Sau3AI GATC 2 cut(s) 171, 442
Sau96I GGNCC 3 cut(s) 130, 136, 595
ScrFI CCNGG 4 cut(s) 134, 139, 140, 391
SetI ASST 6 cut(s) 105, 326, 356, 376, 419, 462
SfaNI GCATC 2 cut(s) 32, 554
SgrAI CRCCGGYG 1 cut(s) 362
SmaI CCCGGG 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 266, 557
SrfI GCCCGGGC 1 cut(s) 140
SsiI CCGC 4 cut(s) 62, 143, 153, 181
StyD4I CCNGG 4 cut(s) 132, 137, 138, 389
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 234
TaiI ACGT 3 cut(s) 105, 356, 419
TaqI TCGA 1 cut(s) 303
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 4 cut(s) 10, 31, 487, 512
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TseI GCWGC 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 250, 327, 424
TspGWI ACGGA 2 cut(s) 131, 480
TspMI CCCGGG 1 cut(s) 138
XmaI CCCGGG 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 299
ZraI GACGTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.