Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0012002

Physical Location & Seq

Genomic Coordinates Reverse (-)

10055458 .. 10059661

Gene Structure 4204 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0124600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4002 bp

ATGGCTGAGTATCTTACTTTTGCTACCAAGGAAATGTTGACCAAAGTGGCTTCACTTGCAGCTCAAGAGTTCAGTCTTGTATGGGGATTCAATGGAGAAATAACAAAGCTGCGTGAGTCGCTGCTCATGCTTGAGGCTGTGCTACGAGATGCTGAACATCTGCGACAAGATCAGGGAGAGGCTGTGAAGCTCTGGGTGAAGAAGCTTGGAGACATAGCTCAACATGCCGATGATGTGTTGGATGATTATGGATATGAGCTTCTCCGGCATAAGGTGCAACTGCGAAACCTGATGAAGAAAAAGGTGCAAGACTTTTTTTCCCTCTCCAATCCCATTATATTTCGTATTAAAATGGCACATAAAATTAAGAAGATCAACACCTCTTTGGATGAGTTGAATAAGAAGGCAAGTGCTATTGGCTTAGTTGCTAGACCATCCTTGGGTGCAACAAATTCATATGGTACAAGCATTGACAGGGAAACCTACTCTCTCTTAAAACAAGATGAAAACAATATCATTGGAAGAGACGATGTTGTGGCAGATATAGTTCAAGCCCTGACCAAATCACACCCCAATCAAGAAAGTGATCTCTCGGTAATGGCCATTGTGGGTATGGGAGGGCTTGGAAAGACAACTTTGGCTAAATTAATATATCATGAATCTGAGATAAGTAGGCACTTTGAGAAAAAGATATGGATATGTGTATCCACCCTTTTTGAAGTCAACTCAATTTTGAGAGGGATGTTGGAATCTCTTAAACCAGAATATGCTGCGGTGAAAGCTATAGATGTAATATGTAACATTCTCCAAGAAGAGTTGAAAGGAAAGAGATACCTTCTTATACTTGATGATGTATGGAATGAAGATACTAAAAAATGGAATGATTTGAAGAGTTGTTTGCTAAGAATTACAGACGCCCAAGGAAGCAGCATTGTTGTTACTACCCGCAGTGGCAAAGTTGCAAAAATGGTGGAGACCATTCGTAGGTGTGATTTGGAAAAACTATCAGATGATGAATGTTGGCTCATATTGAAGGATAAAGCAATTCCAATTGGAAGTGTTCCTATACTTGAAGATCAAGAGAGAATTGGAAGGGAAATTGCCAAAAAATGTGGAGGTGTACCATTAGTGGCAAAGGTCTTAGGAAACATTATGCACTCTAAAACGAGCCATGGATGGAATTCAATTGTGGAAAGTAAAATATGGGAATTACCAGAAGAAGAAGACAGAATCATGTCGATTTTAAAGTTGAGTTTTGATGAATTGGAACATTCATCATTGAAACAGTGTTTTGCATACTGCTCAATGTTCATCAAAGATTTTGAAATAGAAAAGACTGACTTGGTCCAACTTTGGATGGCTCAAGGATGGCTTCACTCTTTCCCAACCAAAAGTAATCTAGATATGGAGGATAGAGGCAACGTATATTTTAACATCTTATTAGAAAAGTCATTTTTTCAAGATGTTAGAAAAGACTTCCACGGTGATATTACTGCATGCAAGATGCACGATCTTGTGCATGATCTTGCGGAGCAAGTATCAAACATGACAAACTGGCGCTCACTATTTTCTAAGGGAGAAGCACTTGGGAGCATTTTGCCTAACTTTAGATCTTTACGTGTGTTAAATTTATACAAGGCAGACATTGACAAGTTGCCAAGTTCAATTGGAAAGTTGACACACTTGAGGTATCTGAATGTTATGGAAACAAATGTCAAAACATTTCCAAAATCTATTGGTCAACTTTATTACCTTGAGACGTTTAAAATGCCTTATCATGTTGAAGAGTTCCCAAAGATAATTGCTAATATGATCAACTTGAGGCATGTTTATTTTGGTAAATATGCGAAAGTTCCGGTTGGGATATTGGGAAGATTGAATAATCTCAGATCATTACCTTTTCTGAAGGTGGGTAAGGAGACTGGGACTCGGATTGAGGAACTGGGTGGGTTAAACCATTTGAGAGACACCTTATCTATCAGTAATCTGGAGAATGTAAGAGATGGAGAAGATGCAGTGAAAGCAAAGTTAGTTGAGAAGAAACATATACGGAAGTTAACTCTTGACTGGAATCTTAGTAGACCAAGCTACGATGTTGACAATGAGGATGATGTACTTGAAGGCCTGCAACCACATTGTCATTTGGAAATTTTGAAGATTCAGGGGTTCATGGGTGTTAAGTTTCCATCATGGTTATTGCTAGCCAGCAACTTGAGAGAAATTGAATTAGCAGAATGCAACCAATGCGAAGGAGTCCCAATGCTTGGCCATCTACCCAGTCTTATACATCTTGAGATGGTTGACATGCAGAACCTAAGATACTTGGGGTCTGAGTTTTATGGTTACGATCAGATTTCCAGTGCAAGAACAAGTGACGAGGGAAAGGCTTTGTTTCCTGCATTGAGATCATTACATATCGTGAACGCCGAGAATCTAATAGATTGGATGGAAGCACCAGAAGTGAGGCTGACAGCAAAAAGATTAACGATGTTTCCTTGCCTTGAGAAGCTGATCCTAGAGGGGTGTGAGCAATTGGGAAGTGCTCCCAATCATTTTCCATCTCTCAAGGAGTTGGTGATACGAGGAGTGGACAGCGGCATGCCAATAGCAAGTATTTTAAGCAATAAACTGACTAGTCTCACTCATCTCCAAATAAATGGTGTCGAGGGAGTTACTAGTCTGCCGGAAGGGACATTAAGAAACAACAAGAATCTTGCATCTTTGGTGTTATGGAACTGTTCGGAGTTAAGTTGCATTGCTCCCCATGGATTTGACTGCTGCGCATCTCTCCAGTTACTGAGTATTTGGAGCTGTCCTAAGTTGAGGTATTTACCTGATGGGCTACTTCTACTCTCTCTCAAGACGTTGAGCATAACAGATTGCGACAGTCTAGAGTCCATCCCAATCGTCCCTGAGCACGGTGGTCTCCCATCTCTACGCAGTTTGGGGATCAGTAATTGTCCTCAGTTATCCAGTTTACCACTACAATACTGCACATCTCTTCAAAAATTGGACATAAAGAGCTGCCCAAAGATAACGTCCGTTCCAATTCCATCTGAGGGCCTCCCATCCCTTGCTGAACTGCTGCTTTCGCAGTGTCCTGAGTTATCAAGCCTACCGAGTGGACTAGGCTGCTGCACCTCTCTTGTTTATTTGTCAATAACAGAGTGCCCTAAGGTAACATCCATTCCACTTGACAGTCTCACTACAACCCTCCGATACTTGTCTGTAAGTAATCTAGAGTCTCTTCCAATTTTACACGGCGGCGGCTTCACATCCCTCCGTCGATTGGGAGTCCAGGGATGTCAAAGTACACAAATTGAGCAGCAATTTTGTGCCTTTCTTCAAATCCTCGTCTCTCTTCAGGAGTTGATAATAGAAGATTGCCCTAATCTAGAGACTCTGCCAAGTTTAGACAAGCTGACATCCCTCCATGATTTGGAGATTTATGATTGTTCCCGATTAACATATCTGCCCAGTGGGTTAGCAGTGGCGTCCCCACACGTATTCACCCGTCTCAAGAAATTGAGACTTGGTCCCTTTTGGAATGAGCTGGATTCATTCCCCGCTCTTCAGGTTCTACCACAACTTGCAAGATTGCGTATCTTCGGGTGGCCTAAGCTCAAGTCTCTGCCTGAACAAATTCAACACTTCACTTCTTTGACCGATTTGTCGATAGAGGCCTTTGAGGGAGTGGAGGCTATTCCAGAATGGTTGGGAAACCTTGCATCTCTTCGCTGCTTGTTTTTTGACAGTTGCAAGAATCTGATGTATCTACCGTCCGTCCAAGCTATGCAACGCCTCACCAAATTACATTCGCTATACATCAGCAATTGTCCCCTTTTAAGCGAAAGATGCATCGAGGAGAGCGGCCCAGAGTGGCCTAAGATTTCTCATATTCCAAGTATTCCAGGTAACTCTTTCCTCTCATTCTCAAAAAAAAAAAAGGTTTTCTTTCCTCTACTTCTTTTCCTCAATTGGTCACTATTTAGATCACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0008285 GO:0009605 GO:0009607 GO:0009624 GO:0022622 GO:0032501 GO:0032502 GO:0042127 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0061062 GO:0065007 GO:0097159 GO:0099402 GO:1901363 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

QLQ WRC

Protein Analysis

1333

Amino Acids

150.03

Weight (kDa)

6.27

Isoelectric Point (pI)

49.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	11 - 97	4.5e-21	Rx N-terminal domain
NB-ARC	PF00931	179 - 349	1.1e-34	NB-ARC domain
WHD_DRP	PF23559	436 - 510	1.5e-22	Disease resistance protein Winged helix domain
LRR_14	PF23598	522 - 807	2.7e-30	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	645 - 769	7.5e-37	R13L1/DRL21 LRRs
LRR_14	PF23598	969 - 1202	7.2e-06	Leucine-rich repeat region
LRR_14	PF23598	1190 - 1276	8.6e-06	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	1213 - 1277	2.1e-06	R13L1/DRL21 LRRs

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012002)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36400 AT3G52910
fragaria_vesca	FvH4_6g18130
malus_domestica	MD12G1020500.v1.1 MD14G1017600.v1.1
prunus_persica	Prupe.7G115500_v2.0.a1 Prupe.7G115500_v2.0.a1
pyrus_communis	pycom14g01600
rosa_chinensis	RchiOBHm_Chr3g0472411
rosa_laevigata	RLG00000024082
rosa_multiflora	Rmu_sc0001096.1_g000014
rosa_roxburghii	Rroxscaffold_6G00408940
rosa_rugosa	Rorug03G0124600
rosa_samantha	Rh3AG177000 Rh3BG203000 Rh3CG193300 Rh3CG200500 Rh3DG172100
rosa_wichuraiana	Rw3G016450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3670
Acc16I	TGCGCA	1 cut(s)	2782
AccB7I	CCANNNNNTGG	2 cut(s)	2264, 3439
AccBSI	CCGCTC	2 cut(s)	3569, 3870
AccI	GTMKAC	1 cut(s)	2080
AciI	CCGC	8 cut(s)	773, 946, 1529, 2595, 3264, 3267, 3567, 3870
AclWI	GGATC	2 cut(s)	2506, 2957
AcoI	YGGCCR	2 cut(s)	600, 2266
AcsI	RAATTY	5 cut(s)	451, 1180, 1627, 2148, 3642
AcuI	CTGAAG	3 cut(s)	1925, 3347, 3557
AcyI	GRCGYC	2 cut(s)	915, 3494
AfaI	GTAC	4 cut(s)	463, 1122, 2115, 3313
AfiI	CCNNNNNNNGG	8 cut(s)	271, 440, 984, 2264, 2918, 3059, 3289, 3439
AflIII	ACRYGT	2 cut(s)	1618, 3502
AhlI	ACTAGT	2 cut(s)	2633, 2675
AjnI	CCWGG	2 cut(s)	3297, 3910
Alw21I	GWGCWC	2 cut(s)	2545, 2919
AlwI	GGATC	2 cut(s)	2506, 2957
AlwNI	CAGNNNCTG	1 cut(s)	2797
AoxI	GGCC	8 cut(s)	600, 2122, 2266, 3061, 3614, 3681, 3871, 3881
ApoI	RAATTY	5 cut(s)	451, 1180, 1627, 2148, 3642
ArsI	GACNNNNNNTTYG	2 cut(s)	3023, 3055
AseI	ATTAAT	1 cut(s)	647
Asp700I	GAANNNNTTC	1 cut(s)	3642
AspLEI	GCGC	2 cut(s)	1560, 2783
AspS9I	GGNCC	4 cut(s)	1345, 3061, 3536, 3872
AsuHPI	GGTGA	6 cut(s)	208, 787, 1496, 2587, 3502, 3796
AsuNHI	GCTAGC	1 cut(s)	2200
AvaII	GGWCC	2 cut(s)	1345, 3536
AxyI	CCTNAGG	1 cut(s)	3174
BaeGI	GKGCMC	1 cut(s)	3173
BalI	TGGCCA	2 cut(s)	602, 2268
BbsI	GAAGAC	1 cut(s)	1230
Bbv12I	GWGCWC	2 cut(s)	2545, 2919
BceAI	ACGGC	1 cut(s)	3277
BciT130I	CCWGG	2 cut(s)	3299, 3912
BciVI	GTATCC	1 cut(s)	715
BclI	TGATCA	1 cut(s)	1812
BcuI	ACTAGT	2 cut(s)	2633, 2675
BfmI	CTRYAG	1 cut(s)	783
BfoI	RGCGCY	1 cut(s)	1561
BfuI	GTATCC	1 cut(s)	715
BglII	AGATCT	1 cut(s)	1610
Bme1390I	CCNGG	2 cut(s)	3299, 3912
Bme18I	GGWCC	2 cut(s)	1345, 3536
BmgT120I	GGNCC	4 cut(s)	1345, 3061, 3536, 3872
BmiI	GGNNCC	1 cut(s)	3538
BmrFI	CCNGG	2 cut(s)	3299, 3912
BmrI	ACTGGG	4 cut(s)	1932, 1952, 2271, 3471
BmsI	GCATC	8 cut(s)	139, 1494, 2002, 2726, 2792, 3737, 3845, 3867
BmtI	GCTAGC	1 cut(s)	2204
BmuI	ACTGGG	4 cut(s)	1932, 1952, 2271, 3471
BpiI	GAAGAC	1 cut(s)	1230
BpmI	CTGGAG	2 cut(s)	2009, 2774
Bpu10I	CCTNAGC	2 cut(s)	2913, 3618
BsaAI	YACGTR	2 cut(s)	1619, 3505
BsaHI	GRCGYC	2 cut(s)	915, 3494
BsaI	GGTCTC	2 cut(s)	968, 2930
BsaJI	CCNNGG	7 cut(s)	27, 438, 919, 1171, 1480, 2764, 3298
BsaWI	WCCGGW	1 cut(s)	1855
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bsc4I	CCNNNNNNNGG	8 cut(s)	271, 440, 984, 2264, 2918, 3059, 3289, 3439
Bse1I	ACTGG	9 cut(s)	1559, 1927, 1947, 2072, 2277, 2358, 2791, 2973, 3477
Bse21I	CCTNAGG	1 cut(s)	3174
Bse3DI	GCAATG	1 cut(s)	2754
BseBI	CCWGG	2 cut(s)	3299, 3912
BseDI	CCNNGG	7 cut(s)	27, 438, 919, 1171, 1480, 2764, 3298
BseLI	CCNNNNNNNGG	8 cut(s)	271, 440, 984, 2264, 2918, 3059, 3289, 3439
BseMI	GCAATG	1 cut(s)	2754
BseMII	CTCAG	8 cut(s)	654, 1900, 2322, 2789, 2904, 2978, 3048, 3093
BseNI	ACTGG	9 cut(s)	1559, 1927, 1947, 2072, 2277, 2358, 2791, 2973, 3477
BseRI	GAGGAG	2 cut(s)	2598, 3878
BseSI	GKGCMC	1 cut(s)	3173
BsgI	GTGCAG	2 cut(s)	2977, 3121
BshFI	GGCC	8 cut(s)	602, 2124, 2268, 3063, 3616, 3683, 3873, 3883
BsiHKAI	GWGCWC	2 cut(s)	2545, 2919
BsiSI	CCGG	3 cut(s)	265, 1856, 2684
BslFI	GGGAC	7 cut(s)	1939, 2240, 2704, 2894, 3481, 3522, 3822
BslI	CCNNNNNNNGG	8 cut(s)	271, 440, 984, 2264, 2918, 3059, 3289, 3439
BsmBI	CGTCTC	4 cut(s)	519, 1751, 3361, 3521
BsmFI	GGGAC	7 cut(s)	1939, 2240, 2704, 2894, 3481, 3522, 3822
BsmI	GAATGC	1 cut(s)	2240
BsnI	GGCC	8 cut(s)	602, 2124, 2268, 3063, 3616, 3683, 3873, 3883
Bso31I	GGTCTC	2 cut(s)	968, 2930
Bsp1286I	GDGCHC	3 cut(s)	2545, 2919, 3173
Bsp19I	CCATGG	2 cut(s)	1171, 2764
BspACI	CCGC	8 cut(s)	773, 946, 1529, 2595, 3264, 3267, 3567, 3870
BspANI	GGCC	8 cut(s)	602, 2124, 2268, 3063, 3616, 3683, 3873, 3883
BspCNI	CTCAG	8 cut(s)	655, 1899, 2323, 2790, 2905, 2977, 3049, 3094
BspHI	TCATGA	1 cut(s)	655
BspLI	GGNNCC	1 cut(s)	3538
BspOI	GCTAGC	1 cut(s)	2204
BspPI	GGATC	2 cut(s)	2506, 2957
BspQI	GCTCTTC	1 cut(s)	3576
BspTNI	GGTCTC	2 cut(s)	968, 2930
BsrBI	CCGCTC	2 cut(s)	3569, 3870
BsrDI	GCAATG	1 cut(s)	2754
BsrI	ACTGG	9 cut(s)	1559, 1927, 1947, 2072, 2277, 2358, 2791, 2973, 3477
BssECI	CCNNGG	7 cut(s)	27, 438, 919, 1171, 1480, 2764, 3298
BssNI	GRCGYC	2 cut(s)	915, 3494
BssT1I	CCWWGG	5 cut(s)	27, 438, 919, 1171, 2764
Bst2UI	CCWGG	2 cut(s)	3299, 3912
Bst4CI	ACNGT	8 cut(s)	1287, 1484, 2738, 2888, 2921, 3200, 3755, 3780
Bst6I	CTCTTC	9 cut(s)	517, 807, 884, 1779, 3006, 3252, 3366, 3576, 3738
BstACI	GRCGYC	2 cut(s)	915, 3494
BstAPI	GCANNNNNTGC	2 cut(s)	274, 2244
BstBAI	YACGTR	2 cut(s)	1619, 3505
BstC8I	GCNNGC	5 cut(s)	1498, 2126, 2202, 2206, 2600
BstDSI	CCRYGG	3 cut(s)	1171, 1480, 2764
BstH2I	RGCGCY	1 cut(s)	1561
BstHHI	GCGC	2 cut(s)	1560, 2783
BstNI	CCWGG	2 cut(s)	3299, 3912
BstNSI	RCATGY	5 cut(s)	227, 1500, 1829, 2308, 2602
BstSCI	CCNGG	2 cut(s)	3297, 3910
BstSFI	CTRYAG	1 cut(s)	783
BstSLI	GKGCMC	1 cut(s)	3173
BstV2I	GAAGAC	1 cut(s)	1230
BstX2I	RGATCY	1 cut(s)	1610
BstXI	CCANNNNNNTGG	1 cut(s)	2657
BstYI	RGATCY	1 cut(s)	1610
Bsu36I	CCTNAGG	1 cut(s)	3174
BsuI	GTATCC	1 cut(s)	715
BsuRI	GGCC	8 cut(s)	602, 2124, 2268, 3063, 3616, 3683, 3873, 3883
BtgI	CCRYGG	3 cut(s)	1171, 1480, 2764
BtsI	GCAGTG	4 cut(s)	955, 2022, 3101, 3495
BtsIMutI	CAGTG	7 cut(s)	955, 1292, 2022, 2365, 3101, 3484, 3495
Cac8I	GCNNGC	5 cut(s)	1498, 2126, 2202, 2206, 2600
CaiI	CAGNNNCTG	1 cut(s)	2797
CciI	TCATGA	1 cut(s)	655
CfoI	GCGC	2 cut(s)	1560, 2783
Cfr13I	GGNCC	4 cut(s)	1345, 3061, 3536, 3872
CseI	GACGC	2 cut(s)	923, 3483
Csp6I	GTAC	4 cut(s)	462, 1121, 2114, 3312
CspCI	CAANNNNNGTGG	6 cut(s)	697, 732, 951, 986, 1093, 1128
CviQI	GTAC	4 cut(s)	462, 1121, 2114, 3312
DraI	TTTAAA	2 cut(s)	1245, 1765
DrdI	GACNNNNNNGTC	1 cut(s)	3670
DseDI	GACNNNNNNGTC	1 cut(s)	3670
EaeI	YGGCCR	2 cut(s)	600, 2266
Eam1104I	CTCTTC	9 cut(s)	517, 807, 884, 1779, 3006, 3252, 3366, 3576, 3738
EarI	CTCTTC	9 cut(s)	517, 807, 884, 1779, 3006, 3252, 3366, 3576, 3738
Eco130I	CCWWGG	5 cut(s)	27, 438, 919, 1171, 2764
Eco147I	AGGCCT	2 cut(s)	2124, 3683
Eco31I	GGTCTC	2 cut(s)	968, 2930
Eco47I	GGWCC	2 cut(s)	1345, 3536
Eco57I	CTGAAG	3 cut(s)	1925, 3347, 3557
Eco81I	CCTNAGG	1 cut(s)	3174
EcoO109I	RGGNCCY	1 cut(s)	3061
EcoRI	GAATTC	1 cut(s)	1180
EcoRII	CCWGG	2 cut(s)	3297, 3910
EcoT14I	CCWWGG	5 cut(s)	27, 438, 919, 1171, 2764
EcoT22I	ATGCAT	1 cut(s)	3860
ErhI	CCWWGG	5 cut(s)	27, 438, 919, 1171, 2764
Esp3I	CGTCTC	4 cut(s)	519, 1751, 3361, 3521
FalI	AAGNNNNNCTT	6 cut(s)	1325, 1357, 1356, 1388, 3938, 3970
FaqI	GGGAC	7 cut(s)	1939, 2240, 2704, 2894, 3481, 3522, 3822
FauI	CCCGC	2 cut(s)	953, 3574
FauNDI	CATATG	1 cut(s)	457
FbaI	TGATCA	1 cut(s)	1812
FblI	GTMKAC	1 cut(s)	2080
FspI	TGCGCA	1 cut(s)	2782
GlaI	GCGC	2 cut(s)	1559, 2782
GsuI	CTGGAG	2 cut(s)	2009, 2774
HaeII	RGCGCY	1 cut(s)	1561
HaeIII	GGCC	8 cut(s)	602, 2124, 2268, 3063, 3616, 3683, 3873, 3883
HapII	CCGG	3 cut(s)	265, 1856, 2684
HgaI	GACGC	2 cut(s)	923, 3483
HhaI	GCGC	2 cut(s)	1560, 2783
Hin1I	GRCGYC	2 cut(s)	915, 3494
Hin6I	GCGC	2 cut(s)	1558, 2781
HinP1I	GCGC	2 cut(s)	1558, 2781
HincII	GTYRAC	7 cut(s)	39, 724, 1677, 1742, 2058, 2098, 2302
HindII	GTYRAC	7 cut(s)	39, 724, 1677, 1742, 2058, 2098, 2302
HindIII	AAGCTT	1 cut(s)	203
HpaI	GTTAAC	1 cut(s)	2058
HpaII	CCGG	3 cut(s)	265, 1856, 2684
HphI	GGTGA	6 cut(s)	208, 787, 1496, 2587, 3502, 3796
Hpy99I	CGWCG	1 cut(s)	3288
HpyAV	CCTTC	8 cut(s)	397, 845, 1027, 1086, 1900, 2114, 2243, 2681
HpyCH4III	ACNGT	8 cut(s)	1287, 1484, 2738, 2888, 2921, 3200, 3755, 3780
HpyCH4IV	ACGT	6 cut(s)	1422, 1618, 1760, 2864, 3038, 3504
HpySE526I	ACGT	6 cut(s)	1422, 1618, 1760, 2864, 3038, 3504
Hsp92I	GRCGYC	2 cut(s)	915, 3494
HspAI	GCGC	2 cut(s)	1558, 2781
Ksp22I	TGATCA	1 cut(s)	1812
KspAI	GTTAAC	1 cut(s)	2058
LguI	GCTCTTC	1 cut(s)	3576
LmnI	GCTCC	5 cut(s)	1531, 1590, 2548, 2764, 2808
LweI	GCATC	8 cut(s)	139, 1494, 2002, 2726, 2792, 3737, 3845, 3867
MaeII	ACGT	6 cut(s)	1422, 1618, 1760, 2864, 3038, 3504
MaeIII	GTNAC	9 cut(s)	797, 937, 2342, 2372, 2671, 2793, 3178, 3914, 3981
MbiI	CCGCTC	2 cut(s)	3569, 3870
MfeI	CAATTG	6 cut(s)	1050, 1185, 1665, 2531, 3832, 3976
MflI	RGATCY	1 cut(s)	1610
MhlI	GDGCHC	3 cut(s)	2545, 2919, 3173
MlsI	TGGCCA	2 cut(s)	602, 2268
MluNI	TGGCCA	2 cut(s)	602, 2268
MlyI	GAGTC	7 cut(s)	125, 1921, 2262, 2903, 3251, 3303, 3394
MmeI	TCCRAC	3 cut(s)	219, 726, 1372
Mox20I	TGGCCA	2 cut(s)	602, 2268
Mph1103I	ATGCAT	1 cut(s)	3860
MroXI	GAANNNNTTC	1 cut(s)	3642
MscI	TGGCCA	2 cut(s)	602, 2268
MslI	CAYNNNNRTG	1 cut(s)	228
Msp20I	TGGCCA	2 cut(s)	602, 2268
MspA1I	CMGCKG	1 cut(s)	2595
MspI	CCGG	3 cut(s)	265, 1856, 2684
MspR9I	CCNGG	2 cut(s)	3299, 3912
MunI	CAATTG	6 cut(s)	1050, 1185, 1665, 2531, 3832, 3976
Mva1269I	GAATGC	1 cut(s)	2240
MvaI	CCWGG	2 cut(s)	3299, 3912
NcoI	CCATGG	2 cut(s)	1171, 2764
NdeI	CATATG	1 cut(s)	457
NheI	GCTAGC	1 cut(s)	2200
NlaIV	GGNNCC	1 cut(s)	3538
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	2 cut(s)	2372, 3981
NsbI	TGCGCA	1 cut(s)	2782
NsiI	ATGCAT	1 cut(s)	3860
NspI	RCATGY	5 cut(s)	227, 1500, 1829, 2308, 2602
PaeI	GCATGC	2 cut(s)	1500, 2602
PagI	TCATGA	1 cut(s)	655
PceI	AGGCCT	2 cut(s)	2124, 3683
PciSI	GCTCTTC	1 cut(s)	3576
PcsI	WCGNNNNNNNCGW	1 cut(s)	2424
PctI	GAATGC	1 cut(s)	2240
PdmI	GAANNNNTTC	1 cut(s)	3642
PflFI	GACNNNGTC	2 cut(s)	1343, 3534
PflMI	CCANNNNNTGG	2 cut(s)	2264, 3439
PleI	GAGTC	7 cut(s)	124, 1921, 2261, 2902, 3250, 3302, 3394
PpsI	GAGTC	7 cut(s)	124, 1921, 2261, 2902, 3250, 3302, 3394
Ppu21I	YACGTR	2 cut(s)	1619, 3505
PshBI	ATTAAT	1 cut(s)	647
Psp6I	CCWGG	2 cut(s)	3297, 3910
PspGI	CCWGG	2 cut(s)	3297, 3910
PspN4I	GGNNCC	1 cut(s)	3538
PspPI	GGNCC	4 cut(s)	1345, 3061, 3536, 3872
PsrI	GAACNNNNNNTAC	2 cut(s)	2303, 2335
PstNI	CAGNNNCTG	1 cut(s)	2797
PsuI	RGATCY	1 cut(s)	1610
PsyI	GACNNNGTC	2 cut(s)	1343, 3534
RsaI	GTAC	4 cut(s)	463, 1122, 2115, 3313
RsaNI	GTAC	4 cut(s)	462, 1121, 2114, 3312
RseI	CAYNNNNRTG	1 cut(s)	228
SapI	GCTCTTC	1 cut(s)	3576
Sau96I	GGNCC	4 cut(s)	1345, 3061, 3536, 3872
SchI	GAGTC	7 cut(s)	125, 1921, 2262, 2903, 3251, 3303, 3394
ScrFI	CCNGG	2 cut(s)	3299, 3912
SduI	GDGCHC	3 cut(s)	2545, 2919, 3173
SfaNI	GCATC	8 cut(s)	139, 1494, 2002, 2726, 2792, 3737, 3845, 3867
SfcI	CTRYAG	1 cut(s)	783
SinI	GGWCC	2 cut(s)	1345, 3536
SmiMI	CAYNNNNRTG	1 cut(s)	228
SpeI	ACTAGT	2 cut(s)	2633, 2675
SphI	GCATGC	2 cut(s)	1500, 2602
SseBI	AGGCCT	2 cut(s)	2124, 3683
SsiI	CCGC	8 cut(s)	773, 946, 1529, 2595, 3264, 3267, 3567, 3870
StuI	AGGCCT	2 cut(s)	2124, 3683
StyD4I	CCNGG	2 cut(s)	3297, 3910
StyI	CCWWGG	5 cut(s)	27, 438, 919, 1171, 2764
TaaI	ACNGT	8 cut(s)	1287, 1484, 2738, 2888, 2921, 3200, 3755, 3780
TaiI	ACGT	6 cut(s)	1425, 1621, 1763, 2867, 3041, 3507
TaqI	TCGA	5 cut(s)	1238, 2664, 3286, 3674, 3861
TaqII	GACCGA	1 cut(s)	3680
TatI	WGTACW	2 cut(s)	2113, 3311
TauI	GCSGC	4 cut(s)	2598, 3267, 3270, 3873
TscAI	CASTG	7 cut(s)	955, 1292, 2022, 2365, 3101, 3484, 3495
TseFI	GTSAC	2 cut(s)	2372, 3981
Tsp45I	GTSAC	2 cut(s)	2372, 3981
TspGWI	ACGGA	4 cut(s)	2065, 3031, 3272, 3772
TspRI	CASTG	7 cut(s)	955, 1292, 2022, 2365, 3101, 3484, 3495
Tth111I	GACNNNGTC	2 cut(s)	1343, 3534
Van91I	CCANNNNNTGG	2 cut(s)	2264, 3439
VpaK11BI	GGWCC	2 cut(s)	1345, 3536
VspI	ATTAAT	1 cut(s)	647
XapI	RAATTY	5 cut(s)	451, 1180, 1627, 2148, 3642
XbaI	TCTAGA	4 cut(s)	1399, 2890, 3238, 3394
XceI	RCATGY	5 cut(s)	227, 1500, 1829, 2308, 2602
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XmiI	GTMKAC	1 cut(s)	2080
XmnI	GAANNNNTTC	1 cut(s)	3642
Zsp2I	ATGCAT	1 cut(s)	3860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.