Rorug03G0244000

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0016769
Physical Location & Seq
Reverse (-)
22786717 .. 22789169
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0244000.1

Sequence Viewer

Length: 2367 bp
ATGGATCAGCTTCAACATTTCAATCACGTCCATCCATTGATGTTTAAGGAAGGGAAGCAGAAAAATCAAGACGGTGCAGCAGTAGTTTGCTCTGCATGTGGAGACCCAGTGCTTGGTCCTAGCTACAGTTGCAGCCAGTGTTCCAAATTATTCATTCTCCACAAGTCATGCGCGGTGCTTCCCCGTGAGATGCACCACCCAATACACCGCAAACATCCACTTATTCTCCTCTCAGGTAGATATTATAGTTGTGATGTATGTCGCAAATCTCATTCCGGCTTATTCTCTTACAATTGTAAGCCATGTGACTTTGACATTGACCTCCAATGTGCTTCCAATTTGAGCAATATAAAATGCTTGGAACACTTCAGTCATGAGCATGAACTGATCTTTATGGAAGAGCCTAAAAGGAAAGGCAAGGATTCACTAGTTGTTTGTGATGGGTGCCGAGAGCAAGTGTTTGGTCCAAGCTACTGTTGCAGCCATTATCATTGCTACTACACTTTGCACAAGTCATGCACGGAGCTTCCCCAGGAGTTTCGTCACCCGATGCATACCCAACATCCACTTATTCTAAACAAAGATAGAGGACGAGAATACCAATGTGATGCGTGTAACCAAGATTGCAGCGGCAGATTCACTTATAGTTGCTTCCAATGTGACTTTAACCTAGACCAAAACTGTGCTTTCAATTGGACCGGTTTTAAGCATTTCAGCCATGATCATCCATTATTGTTCGAAAAAGAGCCAAAAAAGGAATACATTGGTCTTCTACTCTGTGATGGCTGCCAAGACCCAATACTCGGCCCTAGTTACACATGCATCAATACCTTCCGACGTCGCAAATGTGGCTTCAATCTCCATCAAAAATGTGCACAGCTGCCCCATGAAATTGAGCACCCGATGCACCGTCAACACCCGCTTTTTCTCCTCAGCATAATTCAAGATATCAGGGTACGCTGCCTTTGTAACGCCTGCAAGCAACTTTGCAAATTCCGATACAACTGTTCCATATGTGACTTCAACCTCCACCTCAAGTGTGCCTCTGATTGGCAAAATATTATTGCAAGTGACTCTCACGAGCATCAGTTCACAATTCTCCGGAAGGAAATGTCTGACCTCCAAGTTAACTGTGACGTTTGTGGTGAGTACTGGACTGGCACCGTCTACTACATGTGTAGCATTTGCCAGCTCTTGGTTCATAAGGAATGCACTTCATTACCACGTAACATCAAAATACGTGGCCACCAACACCACCTGAAGCTGACTTATTGGTTCCTTGAAGACATTTACCCCAACCGTCTGTCATGGAAAGTCTGCTCTATAAGCATTGATAAATGTCGCGCTGTTTACTGTTGCAGTGAATGTAGCAGTTATGTTGCCCATGTTGCATGTACGACAGGTGAGTACTCTCGAGAAGAAAGCTTTTTGGACATAGCCCTGAATGATGATGAGTCTGTTGATGCTCCAGAGATCAAACACTTCAGTCACCAACATTTATTAGCCGCTAATGATCCTTGTGAGGTAGTTAAATATGATCACAGAGAAATTACTTGTAAGGGATGCATCCGCCCCATAACTGCCATCAAAGATTCCTTTTACAGCTGCACCAAACAAGAAGACGAGTTATGCACTTTCTTTCTCCATAAGATATGTGCTCAATTACCAAAAAAGATGCTTCTCCCTCTACTTCACCAACACCAGTTCACACTCGTCGCAAGGGCACTTCTATTGGTGGCGTGTTCCAGTGTTATATGTGTGGCACTTTTAACCAAGGCTTTACATATACTTGTGAGGAATGTGCCTTACCAACAGGTGAAACTGTTTTCTACCTTGACCTTCAATGCAGTACTTACTGGGAAAACAAGGCTCTTAAACATGAGTCTCATGTTCACCGACTCCTCCTCAACACAGAATGGGAGGACATTACCTCTTACTGCTATGCACCGATACGACGATCATCCTCTCAAGCTCACATATGCTAGTGTTGAAGATGAGCTGGGTTACTATTGTGAAATATGCGAAGGAAAACGAGATCCAACACATTGGTTCTACCGCTGCAACGATTGTGACTTTGATTGCCATACTCATTGCATTTTGGGGAGGTATCAGCAAGTCAAGCTAGGGAGTACTTACAAGCACGATGCTCATGGTCAACACCTCGTCACCCTTGTTGATAAGTCAAAGAGCCCCATTCCTTTCGATAAGAGAGAAAACATTCTTCCCTGCCAGAAGTGTGGAAAACCTTGTGAAGGATTGGTGTTTGAATGTGAAGAGTGCAATATAAATATCCACCGAGAAGGATGTTGTCAGGTAACAGATTTGGAGAGTTCTGTCAAGTCTCCAAAGAAGAGCTCAGGCAAATGA

Protein Analysis

788

Amino Acids

90.8

Weight (kDa)

7.44

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 8 - 58 3.9e-08 C1 domain
C1_2 PF03107 68 - 111 2.7e-08 C1 domain
C1_2 PF03107 184 - 229 1.4e-09 C1 domain
C1_2 PF03107 238 - 292 2.5e-06 C1 domain
C1_2 PF03107 302 - 348 1.9e-07 C1 domain
C1_2 PF03107 359 - 404 8.2e-07 C1 domain
C1_2 PF03107 650 - 699 2.5e-08 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 841
AccB1I GGYRCC 2 cut(s) 444, 1160
AccB7I CCANNNNNTGG 2 cut(s) 113, 1195
AccI GTMKAC 1 cut(s) 1167
AccII CGCG 2 cut(s) 173, 1344
AccIII TCCGGA 1 cut(s) 1101
AciI CCGC 7 cut(s) 173, 208, 630, 920, 1506, 1570, 2056
AclWI GGATC 3 cut(s) 12, 1508, 2030
AcoI YGGCCR 1 cut(s) 1243
AcsI RAATTY 1 cut(s) 992
AcuI CTGAAG 3 cut(s) 352, 1280, 1468
AcyI GRCGYC 1 cut(s) 838
AfaI GTAC 6 cut(s) 957, 1151, 1396, 1409, 1851, 2131
AfiI CCNNNNNNNGG 5 cut(s) 113, 803, 1050, 1195, 2252
AflIII ACRYGT 1 cut(s) 1173
AgeI ACCGGT 1 cut(s) 698
AhlI ACTAGT 1 cut(s) 427
AjiI CACGTC 1 cut(s) 28
AjnI CCWGG 1 cut(s) 531
Alw21I GWGCWC 4 cut(s) 877, 900, 1660, 2357
Alw26I GTCTC 3 cut(s) 96, 1889, 2346
Alw44I GTGCAC 1 cut(s) 873
AlwI GGATC 3 cut(s) 12, 1508, 2030
Ama87I CYCGRG 1 cut(s) 1413
Aor13HI TCCGGA 1 cut(s) 1101
AoxI GGCC 2 cut(s) 805, 1243
ApaLI GTGCAC 1 cut(s) 873
ApeKI GCWGC 9 cut(s) 77, 132, 480, 627, 786, 880, 960, 1605, 2058
ApoI RAATTY 1 cut(s) 992
AsiGI ACCGGT 1 cut(s) 698
Asp700I GAANNNNTTC 1 cut(s) 2217
AspLEI GCGC 2 cut(s) 173, 1346
AspS9I GGNCC 4 cut(s) 116, 464, 696, 806
AsuHPI GGTGA 8 cut(s) 536, 1157, 1415, 1481, 1685, 1828, 1885, 2158
AsuII TTCGAA 1 cut(s) 738
AvaI CYCGRG 1 cut(s) 1413
AvaII GGWCC 3 cut(s) 116, 464, 696
BaeGI GKGCMC 2 cut(s) 877, 1726
BaeI ACNNNNGTAYC 2 cut(s) 991, 1024
BalI TGGCCA 1 cut(s) 1245
BanI GGYRCC 2 cut(s) 444, 1160
BanII GRGCYC 2 cut(s) 2192, 2357
BauI CACGAG 1 cut(s) 1079
BbsI GAAGAC 3 cut(s) 761, 1290, 1626
Bbv12I GWGCWC 4 cut(s) 877, 900, 1660, 2357
BbvCI CCTCAGC 1 cut(s) 932
BbvI GCAGC 9 cut(s) 89, 144, 492, 639, 773, 867, 947, 1592, 2045
BccI CCATC 5 cut(s) 39, 434, 776, 870, 1592
BciT130I CCWGG 1 cut(s) 533
BclI TGATCA 2 cut(s) 721, 1537
BcoDI GTCTC 3 cut(s) 96, 1889, 2346
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 6 cut(s) 120, 428, 671, 810, 1983, 2123
BfmI CTRYAG 1 cut(s) 124
BmcAI AGTACT 4 cut(s) 1151, 1409, 1851, 2131
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 3 cut(s) 116, 464, 696
BmeT110I CYCGRG 1 cut(s) 1413
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 4 cut(s) 116, 464, 696, 806
BmiI GGNNCC 3 cut(s) 446, 1162, 1277
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 2 cut(s) 101, 1866
BmuI ACTGGG 2 cut(s) 101, 1866
BpiI GAAGAC 3 cut(s) 761, 1290, 1626
BpmI CTGGAG 1 cut(s) 1452
Bpu10I CCTNAGC 2 cut(s) 932, 2356
Bpu14I TTCGAA 1 cut(s) 738
BpuEI CTTGAG 2 cut(s) 1019, 1952
BsaAI YACGTR 2 cut(s) 1226, 1241
BsaHI GRCGYC 1 cut(s) 838
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 531, 1773
BsaWI WCCGGW 2 cut(s) 698, 1101
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 210, 240
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 803, 1050, 1195, 2252
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 7 cut(s) 107, 136, 1157, 1162, 1702, 1746, 1861
Bse3DI GCAATG 2 cut(s) 490, 2089
BseAI TCCGGA 1 cut(s) 1101
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 531, 1773
BseGI GGATG 8 cut(s) 31, 214, 562, 724, 1566, 1568, 1960, 2309
BseLI CCNNNNNNNGG 5 cut(s) 113, 803, 1050, 1195, 2252
BseMI GCAATG 2 cut(s) 490, 2089
BseMII CTCAG 2 cut(s) 246, 946
BseNI ACTGG 7 cut(s) 107, 136, 1157, 1162, 1702, 1746, 1861
BseRI GAGGAG 4 cut(s) 218, 920, 1891, 1894
BseSI GKGCMC 2 cut(s) 877, 1726
BseXI GCAGC 9 cut(s) 89, 144, 492, 639, 773, 867, 947, 1592, 2045
BseYI CCCAGC 1 cut(s) 1999
BsgI GTGCAG 2 cut(s) 96, 1591
Bsh1236I CGCG 2 cut(s) 173, 1344
BshFI GGCC 2 cut(s) 807, 1245
BshNI GGYRCC 2 cut(s) 444, 1160
BshTI ACCGGT 1 cut(s) 698
BsiHKAI GWGCWC 4 cut(s) 877, 900, 1660, 2357
BsiHKCI CYCGRG 1 cut(s) 1413
BsiSI CCGG 3 cut(s) 276, 699, 1102
BslI CCNNNNNNNGG 5 cut(s) 113, 803, 1050, 1195, 2252
BsmAI GTCTC 3 cut(s) 96, 1889, 2346
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 2 cut(s) 807, 1245
Bso31I GGTCTC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 1413
Bsp119I TTCGAA 1 cut(s) 738
Bsp1286I GDGCHC 6 cut(s) 877, 900, 1660, 1726, 2192, 2357
Bsp13I TCCGGA 1 cut(s) 1101
Bsp143I GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
BspACI CCGC 7 cut(s) 173, 208, 630, 920, 1506, 1570, 2056
BspANI GGCC 2 cut(s) 807, 1245
BspCNI CTCAG 2 cut(s) 245, 945
BspEI TCCGGA 1 cut(s) 1101
BspFNI CGCG 2 cut(s) 173, 1344
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 446, 1162, 1277
BspPI GGATC 3 cut(s) 12, 1508, 2030
BspQI GCTCTTC 2 cut(s) 393, 2345
BspT104I TTCGAA 1 cut(s) 738
BspT107I GGYRCC 2 cut(s) 444, 1160
BspTNI GGTCTC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 490, 2089
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 7 cut(s) 107, 136, 1157, 1162, 1702, 1746, 1861
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 2 cut(s) 531, 1773
BssMI GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
BssNI GRCGYC 1 cut(s) 838
BssSI CACGAG 1 cut(s) 1079
BssT1I CCWWGG 1 cut(s) 1773
Bst2BI CACGAG 1 cut(s) 1079
Bst2UI CCWGG 1 cut(s) 533
Bst6I CTCTTC 3 cut(s) 393, 2268, 2345
BstACI GRCGYC 1 cut(s) 838
BstAPI GCANNNNNTGC 1 cut(s) 904
BstBAI YACGTR 2 cut(s) 1226, 1241
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 976, 980, 1190
BstDEI CTNAG 3 cut(s) 232, 932, 2356
BstENI CCTNNNNNAGG 1 cut(s) 2250
BstF5I GGATG 8 cut(s) 31, 214, 562, 724, 1566, 1568, 1960, 2309
BstFNI CGCG 2 cut(s) 173, 1344
BstHHI GCGC 2 cut(s) 173, 1346
BstKTI GATC 8 cut(s) 7, 390, 724, 1476, 1516, 1540, 1960, 2038
BstMAI GTCTC 3 cut(s) 96, 1889, 2346
BstMBI GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
BstMWI GCNNNNNNNGC 7 cut(s) 129, 477, 849, 904, 1326, 1388, 2119
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 4 cut(s) 99, 822, 1177, 1395
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 124
BstSLI GKGCMC 2 cut(s) 877, 1726
BstUI CGCG 2 cut(s) 173, 1344
BstV1I GCAGC 9 cut(s) 89, 144, 492, 639, 773, 867, 947, 1592, 2045
BstV2I GAAGAC 3 cut(s) 761, 1290, 1626
BstX2I RGATCY 1 cut(s) 2035
BstXI CCANNNNNNTGG 2 cut(s) 2046, 2237
BstYI RGATCY 1 cut(s) 2035
BsuRI GGCC 2 cut(s) 807, 1245
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 8 cut(s) 31, 214, 562, 724, 1566, 1568, 1960, 2309
BtsI GCAGTG 1 cut(s) 1366
BtsIMutI CAGTG 4 cut(s) 114, 143, 1366, 1753
Cac8I GCNNGC 3 cut(s) 976, 980, 1190
CciI TCATGA 1 cut(s) 373
CfoI GCGC 2 cut(s) 173, 1346
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 4 cut(s) 116, 464, 696, 806
Csp6I GTAC 6 cut(s) 956, 1150, 1395, 1408, 1850, 2130
CspAI ACCGGT 1 cut(s) 698
CspCI CAANNNNNGTGG 2 cut(s) 1222, 1257
CviQI GTAC 6 cut(s) 956, 1150, 1395, 1408, 1850, 2130
DdeI CTNAG 3 cut(s) 232, 932, 2356
DpnI GATC 8 cut(s) 6, 389, 723, 1475, 1515, 1539, 1959, 2037
DpnII GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
EaeI YGGCCR 1 cut(s) 1243
Eam1104I CTCTTC 3 cut(s) 393, 2268, 2345
EarI CTCTTC 3 cut(s) 393, 2268, 2345
EciI GGCGGA 1 cut(s) 1559
Ecl136II GAGCTC 1 cut(s) 2355
Eco130I CCWWGG 1 cut(s) 1773
Eco24I GRGCYC 2 cut(s) 2192, 2357
Eco31I GGTCTC 1 cut(s) 96
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 3 cut(s) 116, 464, 696
Eco53kI GAGCTC 1 cut(s) 2355
Eco57I CTGAAG 3 cut(s) 352, 1280, 1468
Eco88I CYCGRG 1 cut(s) 1413
EcoICRI GAGCTC 1 cut(s) 2355
EcoNI CCTNNNNNAGG 1 cut(s) 2250
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 1773
EcoT22I ATGCAT 3 cut(s) 555, 824, 1568
EcoT38I GRGCYC 2 cut(s) 2192, 2357
ErhI CCWWGG 1 cut(s) 1773
FauI CCCGC 1 cut(s) 927
FauNDI CATATG 2 cut(s) 1013, 1978
FbaI TGATCA 2 cut(s) 721, 1537
FblI GTMKAC 1 cut(s) 1167
FokI GGATG 8 cut(s) 18, 201, 549, 711, 1553, 1575, 1947, 2316
FriOI GRGCYC 2 cut(s) 2192, 2357
FspBI CTAG 6 cut(s) 120, 428, 671, 810, 1983, 2123
GlaI GCGC 2 cut(s) 172, 1345
GsaI CCCAGC 1 cut(s) 2003
GsuI CTGGAG 1 cut(s) 1452
HaeIII GGCC 2 cut(s) 807, 1245
HapII CCGG 3 cut(s) 276, 699, 1102
HhaI GCGC 2 cut(s) 173, 1346
Hin1I GRCGYC 1 cut(s) 838
Hin6I GCGC 2 cut(s) 171, 1344
HinP1I GCGC 2 cut(s) 171, 1344
HincII GTYRAC 3 cut(s) 914, 1129, 2156
HindII GTYRAC 3 cut(s) 914, 1129, 2156
HindIII AAGCTT 1 cut(s) 1423
HinfI GANTC 7 cut(s) 422, 636, 1073, 1454, 1592, 1882, 1898
HpaI GTTAAC 1 cut(s) 1129
HpaII CCGG 3 cut(s) 276, 699, 1102
HphI GGTGA 8 cut(s) 536, 1157, 1415, 1481, 1685, 1828, 1885, 2158
Hpy166II GTNNAC 9 cut(s) 875, 914, 1092, 1129, 1168, 1351, 1707, 1893, 2156
Hpy188I TCNGA 4 cut(s) 836, 998, 1048, 1117
Hpy188III TCNNGA 8 cut(s) 68, 374, 944, 1079, 1102, 1413, 1415, 1469
Hpy8I GTNNAC 9 cut(s) 875, 914, 1092, 1129, 1168, 1351, 1707, 1893, 2156
Hpy99I CGWCG 4 cut(s) 840, 843, 1718, 1958
HpyAV CCTTC 7 cut(s) 44, 841, 1099, 1849, 2018, 2246, 2294
HpyCH4IV ACGT 5 cut(s) 27, 838, 1137, 1225, 1240
HpyF10VI GCNNNNNNNGC 7 cut(s) 129, 477, 849, 904, 1326, 1388, 2119
HpyF3I CTNAG 3 cut(s) 232, 932, 2356
HpySE526I ACGT 5 cut(s) 27, 838, 1137, 1225, 1240
Hsp92I GRCGYC 1 cut(s) 838
HspAI GCGC 2 cut(s) 171, 1344
Kpn2I TCCGGA 1 cut(s) 1101
Ksp22I TGATCA 2 cut(s) 721, 1537
KspAI GTTAAC 1 cut(s) 1129
Kzo9I GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
LguI GCTCTTC 2 cut(s) 393, 2345
LmnI GCTCC 2 cut(s) 523, 1471
Lsp1109I GCAGC 9 cut(s) 89, 144, 492, 639, 773, 867, 947, 1592, 2045
MaeI CTAG 6 cut(s) 120, 428, 671, 810, 1983, 2123
MaeII ACGT 5 cut(s) 27, 838, 1137, 1225, 1240
MalI GATC 8 cut(s) 6, 389, 723, 1475, 1515, 1539, 1959, 2037
MboI GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
MboII GAAGA 9 cut(s) 410, 761, 1295, 1430, 1631, 2003, 2213, 2285, 2362
MfeI CAATTG 2 cut(s) 292, 691
MflI RGATCY 1 cut(s) 2035
MhlI GDGCHC 6 cut(s) 877, 900, 1660, 1726, 2192, 2357
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 4 cut(s) 1067, 1463, 1891, 1892
MmeI TCCRAC 2 cut(s) 859, 2063
Mox20I TGGCCA 1 cut(s) 1245
Mph1103I ATGCAT 3 cut(s) 555, 824, 1568
MroI TCCGGA 1 cut(s) 1101
MroXI GAANNNNTTC 1 cut(s) 2217
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 7 cut(s) 45, 666, 705, 1128, 1530, 1769, 1874
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 4 cut(s) 630, 880, 1605, 2058
MspI CCGG 3 cut(s) 276, 699, 1102
MspR9I CCNGG 1 cut(s) 533
MunI CAATTG 2 cut(s) 292, 691
Mva1269I GAATGC 1 cut(s) 1214
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 2 cut(s) 173, 1344
MwoI GCNNNNNNNGC 7 cut(s) 129, 477, 849, 904, 1326, 1388, 2119
NdeI CATATG 2 cut(s) 1013, 1978
NdeII GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
NlaIV GGNNCC 3 cut(s) 446, 1162, 1277
NmeAIII GCCGAG 2 cut(s) 473, 783
NmuCI GTSAC 9 cut(s) 305, 542, 659, 1016, 1070, 1133, 1487, 2069, 2164
NsiI ATGCAT 3 cut(s) 555, 824, 1568
NspI RCATGY 4 cut(s) 99, 822, 1177, 1395
NspV TTCGAA 1 cut(s) 738
PaeR7I CTCGAG 1 cut(s) 1413
PagI TCATGA 1 cut(s) 373
PciI ACATGT 1 cut(s) 1173
PciSI GCTCTTC 2 cut(s) 393, 2345
PctI GAATGC 1 cut(s) 1214
PdmI GAANNNNTTC 1 cut(s) 2217
PfeI GAWTC 3 cut(s) 422, 636, 1592
PflMI CCANNNNNTGG 2 cut(s) 113, 1195
PinAI ACCGGT 1 cut(s) 698
PleI GAGTC 4 cut(s) 1067, 1462, 1890, 1892
PpsI GAGTC 4 cut(s) 1067, 1462, 1890, 1892
Ppu21I YACGTR 2 cut(s) 1226, 1241
PscI ACATGT 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 2357
Psp6I CCWGG 1 cut(s) 531
PspFI CCCAGC 1 cut(s) 1999
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 446, 1162, 1277
PspPI GGNCC 4 cut(s) 116, 464, 696, 806
PsuI RGATCY 1 cut(s) 2035
PvuII CAGCTG 2 cut(s) 880, 1605
RsaI GTAC 6 cut(s) 957, 1151, 1396, 1409, 1851, 2131
RsaNI GTAC 6 cut(s) 956, 1150, 1395, 1408, 1850, 2130
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 2357
SapI GCTCTTC 2 cut(s) 393, 2345
SaqAI TTAA 7 cut(s) 45, 666, 705, 1128, 1530, 1769, 1874
Sau3AI GATC 8 cut(s) 4, 387, 721, 1473, 1513, 1537, 1957, 2035
Sau96I GGNCC 4 cut(s) 116, 464, 696, 806
ScaI AGTACT 4 cut(s) 1151, 1409, 1851, 2131
SchI GAGTC 4 cut(s) 1067, 1463, 1891, 1892
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 6 cut(s) 877, 900, 1660, 1726, 2192, 2357
SfcI CTRYAG 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 1413
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 3 cut(s) 116, 464, 696
SlaI CTCGAG 1 cut(s) 1413
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 3 cut(s) 1034, 1413, 1967
SmoI CTYRAG 3 cut(s) 1034, 1413, 1967
SpeI ACTAGT 1 cut(s) 427
SsiI CCGC 7 cut(s) 173, 208, 630, 920, 1506, 1570, 2056
SspI AATATT 1 cut(s) 1060
SspMI CTAG 6 cut(s) 120, 428, 671, 810, 1983, 2123
SstI GAGCTC 1 cut(s) 2357
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 1 cut(s) 1773
TaiI ACGT 5 cut(s) 30, 841, 1140, 1228, 1243
TaqI TCGA 3 cut(s) 738, 1414, 2202
TatI WGTACW 4 cut(s) 1149, 1407, 1849, 2129
TauI GCSGC 2 cut(s) 633, 1508
TfiI GAWTC 3 cut(s) 422, 636, 1592
Tru1I TTAA 7 cut(s) 45, 666, 705, 1128, 1530, 1769, 1874
Tru9I TTAA 7 cut(s) 45, 666, 705, 1128, 1530, 1769, 1874
TscAI CASTG 4 cut(s) 114, 143, 1366, 1753
TseFI GTSAC 9 cut(s) 305, 542, 659, 1016, 1070, 1133, 1487, 2069, 2164
TseI GCWGC 9 cut(s) 77, 132, 480, 627, 786, 880, 960, 1605, 2058
Tsp45I GTSAC 9 cut(s) 305, 542, 659, 1016, 1070, 1133, 1487, 2069, 2164
TspDTI ATGAA 5 cut(s) 142, 396, 903, 1190, 1206
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 4 cut(s) 114, 143, 1366, 1753
Van91I CCANNNNNTGG 2 cut(s) 113, 1195
VneI GTGCAC 1 cut(s) 873
VpaK11BI GGWCC 3 cut(s) 116, 464, 696
XagI CCTNNNNNAGG 1 cut(s) 2250
XapI RAATTY 1 cut(s) 992
XceI RCATGY 4 cut(s) 99, 822, 1177, 1395
XhoI CTCGAG 1 cut(s) 1413
XmiI GTMKAC 1 cut(s) 1167
XmnI GAANNNNTTC 1 cut(s) 2217
XspI CTAG 6 cut(s) 120, 428, 671, 810, 1983, 2123
ZraI GACGTC 1 cut(s) 839
ZrmI AGTACT 4 cut(s) 1151, 1409, 1851, 2131
Zsp2I ATGCAT 3 cut(s) 555, 824, 1568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.