Rorug04G0077900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017148
Physical Location & Seq
Reverse (-)
12006310 .. 12008657
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0077900.1

Sequence Viewer

Length: 1185 bp
ATGGTCACTGGTAAGCAGGATGAGGAGCTCATTCTCACAACTGATGAAACACTTGATGAGTCTGGTGATCAGGAGAATAGACAAGTGATCATTGATGAGGAAACTGAGGATACTATCATTTACCAGCTCAATGGAATAGGCCAAAAGACCTCAACTGGTGGGTGGTTTATGTCTGAGGGAGAGTCAGTTCTTCTTGCTCATGATGATGGTTCGTGCACCTTCTATGATATTGTAAACTCCGAGGAAAAGGCTTTGTACAAGCCTCCTGCAGGAGTTTCACCTAATATGTGGAGAGATTGCTGGATCATCCGAGCTCCTAGTGCAGGTGGTTGCGCAGGAAGACCTTGTGGACCTCTTATTGTCTCATCTGCCACCTGTCAGAGAGTTGTGAGAATCTATGACATTCGTGATGGTGAGCAAGTTATGAAATGGGATATCCTGAAGCCTGTGTTAACAATGGATAACTCAAGCCCCTTGCAGTGGAGAAACAGAGGAAAAGTTGTTGTAGCTGACATTGAAACAATTTCCATCTGGGATGTGAACTCTCTCAACCCTCAACCTTTATTATCTGTTTCTTCTGCTGGTCGGAAAATCTCTGCTCTTCATGTGAATAACACTGACTCTGAACTTGGTGGCGGAGTTCGCCAAAGAGTAAGTTCAGCAGAAGCAGAAGGAAATGATGGAGTGTTCTGTACCCAGGATTCATTAAGTATTCTAGACTTTCGCAATCCGTCTGGTATAGGTCTGAAAATACCAAAGCTTGGTGTAACAGCACAGTCAGTTTTCTCTCGTGGAGATTCCATCTTCCTTGGCTGCCCCAATGGAAGGTCTGGATGGAAAAAACAGTCCTCTTCAGTCTTCACAAGTGCAACAGTTTTCAATTCGAAAACAAGGCTAATAAGCACTTGCACCTTGCCAGAATCAAATGCTCACAGCCATCACACAGCTATTACGCAAGTTTGGGGGAACTCAAACCTAGTCATGGGTGTTTGTGGATTGGGGCTGTTTGTGTTTGATGCCTTGAAGGATGACGGTGTGCCGTCTTTCACCAATGATAGTGGAAGCACTCAAGATCGTGAAGCCATTGGCCAAGATGACTTGTATGCACCTTCTTTTGATTACTTGGACTCTCTTGCTCTTATCATATCAAGAGATTGCCCGGCATTATGGAGGCGCTTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

42.84

Weight (kDa)

4.76

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_At4g14310 PF25465 114 - 389 3.6e-135 At4g14310 8-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05894 AT2G31480
prunus_persica Prupe.1G190600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407081
rosa_laevigata RLG00000008738
rosa_multiflora Rmu_sc0000600.1_g000001
rosa_roxburghii Rroxscaffold_5G00351810
rosa_rugosa Rorug04G0077900
rosa_samantha Rh4AG142600 Rh4BG140500 Rh4CG149100 Rh4DG136700
rosa_wichuraiana Rw4G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc16I TGCGCA 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 761
AciI CCGC 1 cut(s) 636
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 1087
AcuI CTGAAG 2 cut(s) 461, 837
AfaI GTAC 2 cut(s) 257, 694
AfiI CCNNNNNNNGG 6 cut(s) 269, 323, 480, 761, 825, 982
AgsI TTSAA 3 cut(s) 518, 880, 1024
AjnI CCWGG 1 cut(s) 696
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 6 cut(s) 28, 127, 314, 509, 760, 947
AluI AGCT 6 cut(s) 28, 127, 314, 509, 760, 947
Alw21I GWGCWC 3 cut(s) 30, 218, 316
Alw26I GTCTC 1 cut(s) 367
Alw44I GTGCAC 1 cut(s) 214
AlwI GGATC 1 cut(s) 311
AoxI GGCC 2 cut(s) 139, 1087
ApaLI GTGCAC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 813
AspLEI GCGC 2 cut(s) 335, 1176
AspS9I GGNCC 1 cut(s) 350
AsuC2I CCSGG 1 cut(s) 1160
AsuHPI GGTGA 4 cut(s) 77, 270, 425, 1039
AsuII TTCGAA 1 cut(s) 884
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 218
BalI TGGCCA 1 cut(s) 1089
BanII GRGCYC 2 cut(s) 30, 316
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 2 cut(s) 346, 850
Bbv12I GWGCWC 3 cut(s) 30, 218, 316
BbvI GCAGC 1 cut(s) 800
BccI CCATC 7 cut(s) 200, 404, 536, 674, 809, 828, 945
BceAI ACGGC 1 cut(s) 1024
BciT130I CCWGG 1 cut(s) 698
BciVI GTATCC 1 cut(s) 103
BclI TGATCA 2 cut(s) 67, 87
BcnI CCSGG 1 cut(s) 1160
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 3 cut(s) 318, 716, 977
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 1177
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 103
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 2 cut(s) 698, 1160
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 698, 1160
BmsI GCATC 1 cut(s) 1006
BpiI GAAGAC 2 cut(s) 346, 850
Bpu14I TTCGAA 1 cut(s) 884
BpuEI CTTGAG 2 cut(s) 451, 1053
BpuMI CCSGG 1 cut(s) 1160
BsaJI CCNNGG 3 cut(s) 240, 696, 808
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 6 cut(s) 269, 323, 480, 761, 825, 982
Bse1I ACTGG 2 cut(s) 13, 160
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 3 cut(s) 240, 696, 808
BseGI GGATG 5 cut(s) 25, 306, 541, 839, 1033
BseLI CCNNNNNNNGG 6 cut(s) 269, 323, 480, 761, 825, 982
BseMII CTCAG 2 cut(s) 96, 165
BseNI ACTGG 2 cut(s) 13, 160
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 1 cut(s) 800
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 2 cut(s) 141, 1089
BsiHKAI GWGCWC 3 cut(s) 30, 218, 316
BsiSI CCGG 1 cut(s) 1160
BslI CCNNNNNNNGG 6 cut(s) 269, 323, 480, 761, 825, 982
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 2 cut(s) 141, 1089
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 30, 218, 316
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 4 cut(s) 67, 87, 303, 1072
BspACI CCGC 1 cut(s) 636
BspANI GGCC 2 cut(s) 141, 1089
BspCNI CTCAG 2 cut(s) 97, 166
BspHI TCATGA 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 606
BspT104I TTCGAA 1 cut(s) 884
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 2 cut(s) 13, 160
BssECI CCNNGG 3 cut(s) 240, 696, 808
BssMI GATC 4 cut(s) 67, 87, 303, 1072
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 1 cut(s) 808
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 4 cut(s) 777, 846, 874, 1034
Bst6I CTCTTC 2 cut(s) 606, 856
BstAUI TGTACA 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 884
BstDEI CTNAG 2 cut(s) 105, 174
BstENI CCTNNNNNAGG 2 cut(s) 267, 321
BstF5I GGATG 5 cut(s) 25, 306, 541, 839, 1033
BstH2I RGCGCY 1 cut(s) 1177
BstHHI GCGC 2 cut(s) 335, 1176
BstKTI GATC 4 cut(s) 70, 90, 306, 1075
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 4 cut(s) 67, 87, 303, 1072
BstMWI GCNNNNNNNGC 2 cut(s) 320, 642
BstNI CCWGG 1 cut(s) 698
BstSCI CCNGG 2 cut(s) 696, 1158
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 800
BstV2I GAAGAC 2 cut(s) 346, 850
BstXI CCANNNNNNTGG 1 cut(s) 131
BsuI GTATCC 1 cut(s) 103
BsuRI GGCC 2 cut(s) 141, 1089
BtsCI GGATG 5 cut(s) 25, 306, 541, 839, 1033
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 3 cut(s) 6, 485, 615
BveI ACCTGC 1 cut(s) 314
CciI TCATGA 1 cut(s) 199
CfoI GCGC 2 cut(s) 335, 1176
Cfr13I GGNCC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 256, 693
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 3 cut(s) 200, 605, 982
CviQI GTAC 2 cut(s) 256, 693
DdeI CTNAG 2 cut(s) 105, 174
DpnI GATC 4 cut(s) 69, 89, 305, 1074
DpnII GATC 4 cut(s) 67, 87, 303, 1072
EaeI YGGCCR 1 cut(s) 1087
Eam1104I CTCTTC 2 cut(s) 606, 856
EarI CTCTTC 2 cut(s) 606, 856
EciI GGCGGA 1 cut(s) 651
Ecl136II GAGCTC 2 cut(s) 28, 314
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 2 cut(s) 30, 316
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 350
Eco53kI GAGCTC 2 cut(s) 28, 314
Eco57I CTGAAG 2 cut(s) 461, 837
EcoICRI GAGCTC 2 cut(s) 28, 314
EcoNI CCTNNNNNAGG 2 cut(s) 267, 321
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 2 cut(s) 30, 316
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 3 cut(s) 203, 608, 985
FatI CATG 3 cut(s) 199, 604, 981
FbaI TGATCA 2 cut(s) 67, 87
Fnu4HI GCNGC 1 cut(s) 814
FokI GGATG 5 cut(s) 32, 293, 548, 846, 1040
FriOI GRGCYC 2 cut(s) 30, 316
Fsp4HI GCNGC 1 cut(s) 814
FspBI CTAG 3 cut(s) 318, 716, 977
FspI TGCGCA 1 cut(s) 334
GlaI GCGC 2 cut(s) 334, 1175
GluI GCNGC 1 cut(s) 814
HaeII RGCGCY 1 cut(s) 1177
HaeIII GGCC 2 cut(s) 141, 1089
HapII CCGG 1 cut(s) 1160
HhaI GCGC 2 cut(s) 335, 1176
Hin1II CATG 3 cut(s) 203, 608, 985
Hin6I GCGC 2 cut(s) 333, 1174
HinP1I GCGC 2 cut(s) 333, 1174
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 8 cut(s) 59, 182, 393, 620, 701, 797, 920, 1127
HpaI GTTAAC 1 cut(s) 453
HpaII CCGG 1 cut(s) 1160
HphI GGTGA 4 cut(s) 77, 270, 425, 1039
Hpy166II GTNNAC 5 cut(s) 216, 235, 350, 453, 541
Hpy188I TCNGA 7 cut(s) 175, 241, 311, 381, 588, 625, 747
Hpy188III TCNNGA 9 cut(s) 71, 200, 407, 439, 716, 831, 1070, 1076, 1149
Hpy8I GTNNAC 5 cut(s) 216, 235, 350, 453, 541
HpyAV CCTTC 5 cut(s) 229, 665, 819, 1018, 1119
HpyCH4III ACNGT 4 cut(s) 777, 846, 874, 1034
HpyCH4V TGCA 7 cut(s) 216, 269, 323, 478, 869, 909, 1106
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 642
HpyF3I CTNAG 2 cut(s) 105, 174
Hsp92II CATG 3 cut(s) 203, 608, 985
HspAI GCGC 2 cut(s) 333, 1174
Ksp22I TGATCA 2 cut(s) 67, 87
KspAI GTTAAC 1 cut(s) 453
Kzo9I GATC 4 cut(s) 67, 87, 303, 1072
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 2 cut(s) 25, 319
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 1 cut(s) 1006
MaeI CTAG 3 cut(s) 318, 716, 977
MaeIII GTNAC 2 cut(s) 4, 766
MalI GATC 4 cut(s) 69, 89, 305, 1074
MboI GATC 4 cut(s) 67, 87, 303, 1072
MboII GAAGA 7 cut(s) 182, 351, 567, 593, 796, 843, 850
MhlI GDGCHC 3 cut(s) 30, 218, 316
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 2 cut(s) 522, 880
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 4 cut(s) 68, 191, 614, 1121
MmeI TCCRAC 1 cut(s) 566
Mox20I TGGCCA 1 cut(s) 1089
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 2 cut(s) 452, 707
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 1089
MspI CCGG 1 cut(s) 1160
MspR9I CCNGG 2 cut(s) 698, 1160
MvaI CCWGG 1 cut(s) 698
MwoI GCNNNNNNNGC 2 cut(s) 320, 642
NciI CCSGG 1 cut(s) 1160
NdeII GATC 4 cut(s) 67, 87, 303, 1072
NlaIII CATG 3 cut(s) 203, 608, 985
NmuCI GTSAC 1 cut(s) 4
NsbI TGCGCA 1 cut(s) 334
NspV TTCGAA 1 cut(s) 884
PagI TCATGA 1 cut(s) 199
PaqCI CACCTGC 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 606
PfeI GAWTC 4 cut(s) 393, 701, 797, 920
PflMI CCANNNNNTGG 1 cut(s) 761
PkrI GCNGC 1 cut(s) 815
PleI GAGTC 4 cut(s) 67, 190, 614, 1121
PpsI GAGTC 4 cut(s) 67, 190, 614, 1121
Psp124BI GAGCTC 2 cut(s) 30, 316
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspPI GGNCC 1 cut(s) 350
PstI CTGCAG 1 cut(s) 271
RsaI GTAC 2 cut(s) 257, 694
RsaNI GTAC 2 cut(s) 256, 693
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 2 cut(s) 30, 316
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 2 cut(s) 452, 707
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 4 cut(s) 67, 87, 303, 1072
Sau96I GGNCC 1 cut(s) 350
SbfI CCTGCAGG 1 cut(s) 271
SchI GAGTC 4 cut(s) 68, 191, 614, 1121
ScrFI CCNGG 2 cut(s) 698, 1160
SdaI CCTGCAGG 1 cut(s) 271
SduI GDGCHC 3 cut(s) 30, 218, 316
SfaNI GCATC 1 cut(s) 1006
SfcI CTRYAG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 2 cut(s) 466, 1068
SmoI CTYRAG 2 cut(s) 466, 1068
Sse8387I CCTGCAGG 1 cut(s) 271
Sse9I AATT 2 cut(s) 522, 880
SsiI CCGC 1 cut(s) 636
SspMI CTAG 3 cut(s) 318, 716, 977
SstI GAGCTC 2 cut(s) 30, 316
StyD4I CCNGG 2 cut(s) 696, 1158
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 4 cut(s) 777, 846, 874, 1034
TaqI TCGA 1 cut(s) 884
TasI AATT 2 cut(s) 522, 880
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 4 cut(s) 393, 701, 797, 920
Tru1I TTAA 2 cut(s) 452, 707
Tru9I TTAA 2 cut(s) 452, 707
TscAI CASTG 3 cut(s) 13, 485, 622
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 813
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 4 cut(s) 60, 440, 593, 693
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 3 cut(s) 13, 485, 622
Van91I CCANNNNNTGG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 350
XagI CCTNNNNNAGG 2 cut(s) 267, 321
XbaI TCTAGA 1 cut(s) 715
XspI CTAG 3 cut(s) 318, 716, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.