Rh4DG136700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017148
Physical Location & Seq
Forward (+)
27219841 .. 27222226
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG136700.1

Sequence Viewer

Length: 1179 bp
ATGAACCTAGTACGTACCAAAAAACATGATCCTATATGCAAAAGCAGCAACAAGGAAAAGTCTGTTGCTGCCGTACTTTCCCATTCATCGGAGAAGGAACACCATATTATTAATCAGTTGGGTAAAGAGAGAAGTTTTAGTGATAAAGAGTCGATGTGGTTGTCTTCAGATTGTTCATCACCTTCTGCATTTTTCAGGGAGTATACATGGTCTGGTTCCTTTCATCTCTCACTTGATGAACACAAGAATGTACACACAGATTTGATGGATGAAAAAGTAGCGAGTCGACTACTTAGTTCAGCTATGAATGATGCAAAACCAAGAGAGTCGTTGTGTGATGACTCAACAAGTTGTGGAAACTCTTCAGCTCCTCTTAGCAGCACACCTAGTTCCTCTCAGATTTTAGATTTGGAGAGAATAGGTTACCAAGTATCTTTGTTGAATCTTGATGGGGATGATTACAAATGGATTCCAGATACAGAAGTTGAGTCATATTGTCTTCAATATGCTTTTCACTCTCCATCATACAAGACTAGGTCGGATCACAAAGCTGAGTTCACTAATTCTTGTATTTCTACAGCCACTGAAGCACAAAATGAAAAACCAGGATATTGTTTTCCATCAGATCAAGCTTCAAAAGCTGTAGAGGTTGAAGAGTTGAACACAAATGAACCTCTTTTTTGGCCATTTGCTCAAAAAATTGATTGGAGTGCCGAAGCAACTTGGAACTGTTTTTCCATGTCACCGCGGAAGAGCATAGCAACTATTGGAACTCCACTTGATTCAATTATATTAAGGCGTTATAGTGGCAGCATGGATCTCAAACAAGAGCGTAGGAGAAAACTAGTGTTTAGCTTAGGATCAACAACATCAAAGATACCAGATATGAAACAATGCTACACCAAGGGTATCAAGGATATCGGAGAGATCTGTAATGCCCCTTCGAGGTTGAGCAGGGAAACCAAAAGGACTGAAAAAGACAATATTTTAGAACATCAAAATAAAGAAGTAACTATAAAAAAACCAGTTGTTATGGCTGAAGATATCATGGAAGAAGATTTTGAATCAAAACAACATGGGATTGAGACGTTCTTGGGCCTTGATGAGTTTGATGGTCACGAGGGAATTGATTTAACATTTAACAAAGATGATTTCTCACTGAACATATCTTTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

44.35

Weight (kDa)

5.34

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05894 AT2G31480
prunus_persica Prupe.1G190600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407081
rosa_laevigata RLG00000008738
rosa_multiflora Rmu_sc0000600.1_g000001
rosa_roxburghii Rroxscaffold_5G00351810
rosa_rugosa Rorug04G0077900
rosa_samantha Rh4AG142600 Rh4BG140500 Rh4CG149100 Rh4DG136700
rosa_wichuraiana Rw4G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 203, 286
AccII CGCG 1 cut(s) 748
AciI CCGC 2 cut(s) 746, 748
AclWI GGATC 4 cut(s) 23, 549, 825, 868
AcoI YGGCCR 1 cut(s) 683
AcuI CTGAAG 4 cut(s) 150, 348, 606, 1059
AfaI GTAC 4 cut(s) 12, 16, 75, 252
AfiI CCNNNNNNNGG 2 cut(s) 88, 945
AgsI TTSAA 7 cut(s) 442, 503, 636, 653, 661, 786, 1064
AhlI ACTAGT 1 cut(s) 844
AjnI CCWGG 1 cut(s) 604
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
AluBI AGCT 6 cut(s) 302, 368, 551, 632, 641, 855
AluI AGCT 6 cut(s) 302, 368, 551, 632, 641, 855
Alw26I GTCTC 1 cut(s) 1079
AlwI GGATC 4 cut(s) 23, 549, 825, 868
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 2 cut(s) 683, 1096
ApeKI GCWGC 4 cut(s) 45, 68, 378, 810
AseI ATTAAT 1 cut(s) 111
Asp700I GAANNNNTTC 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 1096
AsuHPI GGTGA 2 cut(s) 171, 735
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BalI TGGCCA 1 cut(s) 685
BarI GAAGNNNNNNTAC 2 cut(s) 925, 957
BauI CACGAG 1 cut(s) 1118
BbsI GAAGAC 2 cut(s) 156, 491
BbvI GCAGC 4 cut(s) 55, 57, 390, 822
BccI CCATC 5 cut(s) 259, 443, 529, 628, 1106
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 1079
BcuI ACTAGT 1 cut(s) 844
BfaI CTAG 4 cut(s) 8, 387, 534, 845
BfmI CTRYAG 2 cut(s) 576, 642
BglII AGATCT 1 cut(s) 927
BisI GCNGC 4 cut(s) 46, 69, 379, 811
BlsI GCNGC 4 cut(s) 47, 70, 380, 812
Bme1390I CCNGG 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 1096
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 2 cut(s) 156, 491
Bpu10I CCTNAGC 1 cut(s) 856
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 746, 903
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 945
Bse1I ACTGG 1 cut(s) 1025
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 746, 903
BseGI GGATG 2 cut(s) 274, 460
BseLI CCNNNNNNNGG 2 cut(s) 88, 945
BseMII CTCAG 2 cut(s) 410, 543
BseNI ACTGG 1 cut(s) 1025
BseRI GAGGAG 1 cut(s) 360
BseXI GCAGC 4 cut(s) 55, 57, 390, 822
Bsh1236I CGCG 1 cut(s) 748
BshFI GGCC 2 cut(s) 685, 1098
BslI CCNNNNNNNGG 2 cut(s) 88, 945
BsmAI GTCTC 1 cut(s) 1079
BsmBI CGTCTC 1 cut(s) 1079
BsnI GGCC 2 cut(s) 685, 1098
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 6 cut(s) 28, 541, 625, 817, 860, 927
BspACI CCGC 2 cut(s) 746, 748
BspANI GGCC 2 cut(s) 685, 1098
BspCNI CTCAG 2 cut(s) 409, 544
BspFNI CGCG 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 4 cut(s) 23, 549, 825, 868
BspQI GCTCTTC 1 cut(s) 746
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 1 cut(s) 1025
BssECI CCNNGG 2 cut(s) 746, 903
BssMI GATC 6 cut(s) 28, 541, 625, 817, 860, 927
BssNAI GTATAC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 1118
BssT1I CCWWGG 1 cut(s) 903
Bst1107I GTATAC 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 1118
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 1 cut(s) 731
Bst6I CTCTTC 3 cut(s) 367, 648, 746
BstAUI TGTACA 1 cut(s) 250
BstBAI YACGTR 1 cut(s) 14
BstDEI CTNAG 5 cut(s) 293, 374, 396, 552, 856
BstDSI CCRYGG 1 cut(s) 746
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 2 cut(s) 274, 460
BstFNI CGCG 1 cut(s) 748
BstKTI GATC 6 cut(s) 31, 544, 628, 820, 863, 930
BstMAI GTCTC 1 cut(s) 1079
BstMBI GATC 6 cut(s) 28, 541, 625, 817, 860, 927
BstMWI GCNNNNNNNGC 3 cut(s) 45, 587, 638
BstNI CCWGG 1 cut(s) 606
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 2 cut(s) 576, 642
BstSNI TACGTA 1 cut(s) 14
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 4 cut(s) 55, 57, 390, 822
BstV2I GAAGAC 2 cut(s) 156, 491
BstX2I RGATCY 2 cut(s) 817, 927
BstYI RGATCY 2 cut(s) 817, 927
BstZ17I GTATAC 1 cut(s) 204
BsuRI GGCC 2 cut(s) 685, 1098
BtgI CCRYGG 1 cut(s) 746
BtsCI GGATG 2 cut(s) 274, 460
BtsIMutI CAGTG 2 cut(s) 582, 1157
CaiI CAGNNNCTG 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 1096
Cfr42I CCGCGG 1 cut(s) 749
Csp6I GTAC 4 cut(s) 11, 15, 74, 251
CviAII CATG 6 cut(s) 26, 207, 739, 814, 1048, 1076
CviQI GTAC 4 cut(s) 11, 15, 74, 251
DdeI CTNAG 5 cut(s) 293, 374, 396, 552, 856
DpnI GATC 6 cut(s) 30, 543, 627, 819, 862, 929
DpnII GATC 6 cut(s) 28, 541, 625, 817, 860, 927
EaeI YGGCCR 1 cut(s) 683
Eam1104I CTCTTC 3 cut(s) 367, 648, 746
EarI CTCTTC 3 cut(s) 367, 648, 746
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 903
Eco32I GATATC 2 cut(s) 919, 1045
Eco57I CTGAAG 4 cut(s) 150, 348, 606, 1059
Eco91I GGTNACC 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 2 cut(s) 919, 1045
EcoT14I CCWWGG 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 903
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 6 cut(s) 29, 210, 742, 817, 1051, 1079
FatI CATG 6 cut(s) 25, 206, 738, 813, 1047, 1075
FblI GTMKAC 2 cut(s) 203, 286
Fnu4HI GCNGC 4 cut(s) 46, 69, 379, 811
FokI GGATG 2 cut(s) 281, 467
Fsp4HI GCNGC 4 cut(s) 46, 69, 379, 811
FspBI CTAG 4 cut(s) 8, 387, 534, 845
GluI GCNGC 4 cut(s) 46, 69, 379, 811
HaeIII GGCC 2 cut(s) 685, 1098
Hin1II CATG 6 cut(s) 29, 210, 742, 817, 1051, 1079
HincII GTYRAC 1 cut(s) 287
HindII GTYRAC 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 9 cut(s) 149, 283, 326, 341, 442, 469, 488, 782, 1064
HphI GGTGA 2 cut(s) 171, 735
Hpy166II GTNNAC 4 cut(s) 204, 253, 287, 558
Hpy188I TCNGA 6 cut(s) 91, 169, 399, 541, 625, 923
Hpy188III TCNNGA 3 cut(s) 446, 473, 1118
Hpy8I GTNNAC 4 cut(s) 204, 253, 287, 558
HpyAV CCTTC 3 cut(s) 88, 192, 951
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4IV ACGT 2 cut(s) 13, 1088
HpyCH4V TGCA 3 cut(s) 39, 188, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 587, 638
HpyF3I CTNAG 5 cut(s) 293, 374, 396, 552, 856
HpySE526I ACGT 2 cut(s) 13, 1088
Hsp92II CATG 6 cut(s) 29, 210, 742, 817, 1051, 1079
KspI CCGCGG 1 cut(s) 749
Kzo9I GATC 6 cut(s) 28, 541, 625, 817, 860, 927
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 8 cut(s) 181, 198, 486, 591, 618, 894, 940, 1038
Lsp1109I GCAGC 4 cut(s) 55, 57, 390, 822
LweI GCATC 1 cut(s) 301
MaeI CTAG 4 cut(s) 8, 387, 534, 845
MaeII ACGT 2 cut(s) 13, 1088
MaeIII GTNAC 4 cut(s) 422, 741, 1009, 1115
MalI GATC 6 cut(s) 30, 543, 627, 819, 862, 929
MboI GATC 6 cut(s) 28, 541, 625, 817, 860, 927
MboII GAAGA 8 cut(s) 156, 354, 491, 665, 763, 1052, 1064, 1067
MflI RGATCY 2 cut(s) 817, 927
MlsI TGGCCA 1 cut(s) 685
MluCI AATT 4 cut(s) 562, 699, 786, 1125
MluNI TGGCCA 1 cut(s) 685
MlyI GAGTC 5 cut(s) 158, 292, 335, 335, 497
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 6 cut(s) 381, 403, 640, 684, 939, 1114
Mox20I TGGCCA 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 361
MscI TGGCCA 1 cut(s) 685
MseI TTAA 4 cut(s) 111, 794, 1133, 1140
MslI CAYNNNNRTG 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 685
MspA1I CMGCKG 1 cut(s) 748
MspR9I CCNGG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 748
MwoI GCNNNNNNNGC 3 cut(s) 45, 587, 638
NdeII GATC 6 cut(s) 28, 541, 625, 817, 860, 927
NlaIII CATG 6 cut(s) 29, 210, 742, 817, 1051, 1079
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 741, 1115
PciSI GCTCTTC 1 cut(s) 746
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 4 cut(s) 442, 469, 782, 1064
PflFI GACNNNGTC 1 cut(s) 535
PkrI GCNGC 4 cut(s) 47, 70, 380, 812
PleI GAGTC 5 cut(s) 157, 291, 334, 335, 496
PpsI GAGTC 5 cut(s) 157, 291, 334, 335, 496
Ppu21I YACGTR 1 cut(s) 14
PshBI ATTAAT 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 604
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 1096
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 2 cut(s) 817, 927
PsyI GACNNNGTC 1 cut(s) 535
RsaI GTAC 4 cut(s) 12, 16, 75, 252
RsaNI GTAC 4 cut(s) 11, 15, 74, 251
RseI CAYNNNNRTG 1 cut(s) 246
SacII CCGCGG 1 cut(s) 749
SalI GTCGAC 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 4 cut(s) 111, 794, 1133, 1140
SatI GCNGC 4 cut(s) 46, 69, 379, 811
Sau3AI GATC 6 cut(s) 28, 541, 625, 817, 860, 927
Sau96I GGNCC 1 cut(s) 1096
SchI GAGTC 5 cut(s) 158, 292, 335, 335, 497
ScrFI CCNGG 1 cut(s) 606
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 2 cut(s) 576, 642
Sfr303I CCGCGG 1 cut(s) 749
SgrBI CCGCGG 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 246
SnaBI TACGTA 1 cut(s) 14
SpeI ACTAGT 1 cut(s) 844
Sse9I AATT 4 cut(s) 562, 699, 786, 1125
SsiI CCGC 2 cut(s) 746, 748
SspI AATATT 1 cut(s) 985
SspMI CTAG 4 cut(s) 8, 387, 534, 845
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 903
TaaI ACNGT 1 cut(s) 731
TaiI ACGT 2 cut(s) 16, 1091
TaqI TCGA 3 cut(s) 152, 286, 944
TasI AATT 4 cut(s) 562, 699, 786, 1125
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 4 cut(s) 442, 469, 782, 1064
Tru1I TTAA 4 cut(s) 111, 794, 1133, 1140
Tru9I TTAA 4 cut(s) 111, 794, 1133, 1140
TscAI CASTG 2 cut(s) 589, 1164
TseFI GTSAC 2 cut(s) 741, 1115
TseI GCWGC 4 cut(s) 45, 68, 378, 810
Tsp45I GTSAC 2 cut(s) 741, 1115
TspRI CASTG 2 cut(s) 589, 1164
Tth111I GACNNNGTC 1 cut(s) 535
VspI ATTAAT 1 cut(s) 111
XmiI GTMKAC 2 cut(s) 203, 286
XmnI GAANNNNTTC 1 cut(s) 361
XspI CTAG 4 cut(s) 8, 387, 534, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.