Rorug04G0174300

CW-type Zinc Finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004853
Physical Location & Seq
Reverse (-)
30664346 .. 30665266
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0174300.1

Sequence Viewer

Length: 921 bp
ATGGGAAACATATTTTCTTACTGTTTCACTTACAAGGAAGCTTCAGATCGGTGGAGCGAGGAGGAAGAAGAAAACAAGCGCCATGAGCAACGAGACAAAGCTTCAGTTGACAGAGTCAGCGGCCTCCCAGATCGGTGCATTTATGAGGAGGAAGAAAACAAGCACCATGAGAAGAGAAACAGTACTAACTTAGCTGACAGAATCAGTGATCTCCCAGATGATATGTTGCATCTCATACTCTCGTTCCTACCTTTCAAATCCGTCGGGCAAACCAGTGTCTTATCTCGAAGATGGAGTCATGTATGGTCTTCCTATCCCATCTTTGACTTCTACGACATTTTTACTGGTAATGAAGTTCTTCATCACCAGACGAGAGCAAGGATCATAAACACAGTATTGGCTCGCCAGGACGAAAACTATAATATAATGTGTCCATGCCTCAAAATTCCCTTCCCAAGCGGCAGTTTAGGTCAATTTCGAAGAGTTGGGGAAGATCCAAACGATGCTTGTTTTGCTAGGGTTGAGCAACTAGTACTGGACATATCCTTGAGTACTGAAAACACATATGTTTTGCCTCAATGCCAACTTAAGTGCCAATCACTAAGGAGTAGTAGCTGTGGGAATTCAAAGGCATGGCTAGGGTTTCCCTCTTCTTATGTTGTTTGGTCTTACCTCCTTTCACTTCAATCTTTGTCTCTAAGTCGTATGGATTTCTTGGATAGTAGTCATAGTACTAGCGCTTTGGATGTGGATTTATTTTCTGGTTCTTCGTTCCCTTGTCTCGAAAAATTGGAGATCTCTATGTGCAAAGGAATGTATGATCTCAAAATTTGCTGTCCGAACCTAAAAGATATACGAGTTTTCGGAATGGATTTAAAGAGTATAGACATCTCCGGAATGAGACTAGAGGATGTAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CW

Protein Analysis

306

Amino Acids

35.16

Weight (kDa)

5.43

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 68 - 107 5.6e-10 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 2 cut(s) 120, 459
AclWI GGATC 2 cut(s) 389, 488
AcsI RAATTY 3 cut(s) 444, 622, 828
AcuI CTGAAG 2 cut(s) 27, 87
AfaI GTAC 4 cut(s) 184, 534, 553, 733
AfeI AGCGCT 1 cut(s) 739
AflII CTTAAG 1 cut(s) 587
AgsI TTSAA 3 cut(s) 256, 627, 686
AhlI ACTAGT 1 cut(s) 529
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 4 cut(s) 41, 101, 194, 615
AluI AGCT 4 cut(s) 41, 101, 194, 615
Alw26I GTCTC 4 cut(s) 87, 699, 785, 895
AlwI GGATC 2 cut(s) 389, 488
Aor13HI TCCGGA 1 cut(s) 893
Aor51HI AGCGCT 1 cut(s) 739
AoxI GGCC 1 cut(s) 121
ApoI RAATTY 3 cut(s) 444, 622, 828
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 2 cut(s) 81, 740
AsuHPI GGTGA 1 cut(s) 356
AsuII TTCGAA 1 cut(s) 478
BbsI GAAGAC 1 cut(s) 300
BccI CCATC 2 cut(s) 285, 326
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 4 cut(s) 87, 699, 785, 895
BcuI ACTAGT 1 cut(s) 529
BfaI CTAG 5 cut(s) 516, 530, 638, 735, 905
BfoI RGCGCY 2 cut(s) 82, 741
BfrI CTTAAG 1 cut(s) 587
BglII AGATCT 1 cut(s) 795
BisI GCNGC 2 cut(s) 121, 460
BlsI GCNGC 2 cut(s) 122, 461
BmcAI AGTACT 4 cut(s) 184, 534, 553, 733
Bme1390I CCNGG 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 2 cut(s) 238, 493
BpiI GAAGAC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 568
BsaWI WCCGGW 1 cut(s) 893
Bse1I ACTGG 3 cut(s) 273, 349, 540
BseAI TCCGGA 1 cut(s) 893
BseBI CCWGG 1 cut(s) 407
BseGI GGATG 2 cut(s) 751, 916
BseNI ACTGG 3 cut(s) 273, 349, 540
BseRI GAGGAG 2 cut(s) 74, 161
BshFI GGCC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 894
BsmAI GTCTC 4 cut(s) 87, 699, 785, 895
BsnI GGCC 1 cut(s) 123
Bsp119I TTCGAA 1 cut(s) 478
Bsp13I TCCGGA 1 cut(s) 893
Bsp143I GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
BspACI CCGC 2 cut(s) 120, 459
BspANI GGCC 1 cut(s) 123
BspEI TCCGGA 1 cut(s) 893
BspPI GGATC 2 cut(s) 389, 488
BspT104I TTCGAA 1 cut(s) 478
BspTI CTTAAG 1 cut(s) 587
BsrI ACTGG 3 cut(s) 273, 349, 540
BssMI GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 3 cut(s) 23, 182, 394
Bst6I CTCTTC 3 cut(s) 167, 475, 655
BstAFI CTTAAG 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 3 cut(s) 190, 602, 698
BstF5I GGATG 2 cut(s) 751, 916
BstH2I RGCGCY 2 cut(s) 82, 741
BstHHI GCGC 2 cut(s) 81, 740
BstKTI GATC 7 cut(s) 49, 133, 211, 384, 496, 798, 823
BstMAI GTCTC 4 cut(s) 87, 699, 785, 895
BstMBI GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
BstMWI GCNNNNNNNGC 2 cut(s) 85, 512
BstNI CCWGG 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 2 cut(s) 493, 795
BstYI RGATCY 2 cut(s) 493, 795
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 751, 916
BtsIMutI CAGTG 2 cut(s) 211, 280
Cac8I GCNNGC 1 cut(s) 403
CfoI GCGC 2 cut(s) 81, 740
Csp6I GTAC 4 cut(s) 183, 533, 552, 732
CviAII CATG 5 cut(s) 83, 167, 299, 435, 633
CviJI RGCY 7 cut(s) 41, 101, 123, 194, 401, 615, 637
CviKI_1 RGCY 7 cut(s) 41, 101, 123, 194, 401, 615, 637
CviQI GTAC 4 cut(s) 183, 533, 552, 732
DdeI CTNAG 3 cut(s) 190, 602, 698
DpnI GATC 7 cut(s) 48, 132, 210, 383, 495, 797, 822
DpnII GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
DraI TTTAAA 1 cut(s) 876
Eam1104I CTCTTC 3 cut(s) 167, 475, 655
EarI CTCTTC 3 cut(s) 167, 475, 655
Eco47III AGCGCT 1 cut(s) 739
Eco57I CTGAAG 2 cut(s) 27, 87
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 405
FaeI CATG 5 cut(s) 86, 170, 302, 438, 636
FatI CATG 5 cut(s) 82, 166, 298, 434, 632
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 121, 460
FokI GGATG 1 cut(s) 758
Fsp4HI GCNGC 2 cut(s) 121, 460
FspBI CTAG 5 cut(s) 516, 530, 638, 735, 905
GlaI GCGC 2 cut(s) 80, 739
GluI GCNGC 2 cut(s) 121, 460
HaeII RGCGCY 2 cut(s) 82, 741
HaeIII GGCC 1 cut(s) 123
HapII CCGG 1 cut(s) 894
HhaI GCGC 2 cut(s) 81, 740
Hin1II CATG 5 cut(s) 86, 170, 302, 438, 636
Hin6I GCGC 2 cut(s) 79, 738
HinP1I GCGC 2 cut(s) 79, 738
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 39, 99
HinfI GANTC 3 cut(s) 114, 201, 295
HpaII CCGG 1 cut(s) 894
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 46, 840, 866
Hpy188III TCNNGA 3 cut(s) 285, 782, 894
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 3 cut(s) 23, 182, 394
HpyCH4V TGCA 3 cut(s) 138, 229, 807
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 512
HpyF3I CTNAG 3 cut(s) 190, 602, 698
Hsp92II CATG 5 cut(s) 86, 170, 302, 438, 636
HspAI GCGC 2 cut(s) 79, 738
Kpn2I TCCGGA 1 cut(s) 893
Kzo9I GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
LmnI GCTCC 1 cut(s) 54
LweI GCATC 2 cut(s) 238, 493
MaeI CTAG 5 cut(s) 516, 530, 638, 735, 905
MalI GATC 7 cut(s) 48, 132, 210, 383, 495, 797, 822
MboI GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
MflI RGATCY 2 cut(s) 493, 795
MluCI AATT 5 cut(s) 444, 473, 622, 788, 828
MlyI GAGTC 2 cut(s) 123, 304
MroI TCCGGA 1 cut(s) 893
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 2 cut(s) 588, 875
MspA1I CMGCKG 1 cut(s) 120
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 1 cut(s) 407
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 85, 512
NdeI CATATG 1 cut(s) 565
NdeII GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
NlaIII CATG 5 cut(s) 86, 170, 302, 438, 636
NspV TTCGAA 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 201
PflFI GACNNNGTC 1 cut(s) 113
PkrI GCNGC 2 cut(s) 122, 461
PleI GAGTC 2 cut(s) 122, 303
PpsI GAGTC 2 cut(s) 122, 303
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PsuI RGATCY 2 cut(s) 493, 795
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 4 cut(s) 184, 534, 553, 733
RsaNI GTAC 4 cut(s) 183, 533, 552, 732
SaqAI TTAA 2 cut(s) 588, 875
SatI GCNGC 2 cut(s) 121, 460
Sau3AI GATC 7 cut(s) 46, 130, 208, 381, 493, 795, 820
ScaI AGTACT 4 cut(s) 184, 534, 553, 733
SchI GAGTC 2 cut(s) 123, 304
ScrFI CCNGG 1 cut(s) 407
SetI ASST 9 cut(s) 43, 103, 196, 253, 472, 617, 675, 846, 920
SfaNI GCATC 2 cut(s) 238, 493
SfuI TTCGAA 1 cut(s) 478
SmlI CTYRAG 2 cut(s) 547, 587
SmoI CTYRAG 2 cut(s) 547, 587
SpeI ACTAGT 1 cut(s) 529
Sse9I AATT 5 cut(s) 444, 473, 622, 788, 828
SsiI CCGC 2 cut(s) 120, 459
SspMI CTAG 5 cut(s) 516, 530, 638, 735, 905
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 23, 182, 394
TaqI TCGA 3 cut(s) 286, 478, 783
TasI AATT 5 cut(s) 444, 473, 622, 788, 828
TatI WGTACW 4 cut(s) 182, 532, 551, 731
TauI GCSGC 2 cut(s) 123, 462
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 2 cut(s) 588, 875
Tru9I TTAA 2 cut(s) 588, 875
TscAI CASTG 2 cut(s) 211, 280
TspDTI ATGAA 2 cut(s) 350, 366
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 2 cut(s) 211, 280
Tth111I GACNNNGTC 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 587
XapI RAATTY 3 cut(s) 444, 622, 828
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 5 cut(s) 516, 530, 638, 735, 905
ZrmI AGTACT 4 cut(s) 184, 534, 553, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.